ATE510005T1 - Verfahren zur isolierung von zellen aus der nabelschnur - Google Patents

Verfahren zur isolierung von zellen aus der nabelschnur

Info

Publication number
ATE510005T1
ATE510005T1 AT03739908T AT03739908T ATE510005T1 AT E510005 T1 ATE510005 T1 AT E510005T1 AT 03739908 T AT03739908 T AT 03739908T AT 03739908 T AT03739908 T AT 03739908T AT E510005 T1 ATE510005 T1 AT E510005T1
Authority
AT
Austria
Prior art keywords
cells
cord
umbilical
umbile
isolation
Prior art date
Application number
AT03739908T
Other languages
English (en)
Inventor
Francois Auger
Lucie Germain
Murielle Remy-Zolghadri
Cindy Hayward
Original Assignee
Univ Laval
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Laval filed Critical Univ Laval
Application granted granted Critical
Publication of ATE510005T1 publication Critical patent/ATE510005T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0691—Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Vascular Medicine (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Dermatology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT03739908T 2003-06-27 2003-06-27 Verfahren zur isolierung von zellen aus der nabelschnur ATE510005T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CA2003/000979 WO2005001081A1 (en) 2003-06-27 2003-06-27 Method of isolating cells from umbilical cord

Publications (1)

Publication Number Publication Date
ATE510005T1 true ATE510005T1 (de) 2011-06-15

Family

ID=33546112

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03739908T ATE510005T1 (de) 2003-06-27 2003-06-27 Verfahren zur isolierung von zellen aus der nabelschnur

Country Status (6)

Country Link
US (2) US20060199263A1 (de)
EP (1) EP1639099B1 (de)
AT (1) ATE510005T1 (de)
AU (1) AU2003304250A1 (de)
CA (1) CA2529718C (de)
WO (1) WO2005001081A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0407221A (pt) * 2003-02-11 2006-01-31 John E Davies Extrato de geléia de wharton, método para a obtenção de uma célula progenitora humana, métodos para a produção de uma população de células, populações de células e método para a produção de tecido ósseo
PL1789534T3 (pl) 2004-08-16 2015-03-31 Cellresearch Corp Pte Ltd Wyodrębnienie komórek macierzystych/progenitorowych z błony owodniowej pępowiny
KR20140146220A (ko) * 2005-12-22 2014-12-24 제인 에니스 동결된 제대조직으로부터 유래하는 생존가능한 세포
US9944900B2 (en) 2006-01-18 2018-04-17 Hemacell Perfusion Pulsatile perfusion extraction method for non-embryonic pluripotent stem cells
AU2007247725B2 (en) * 2006-05-05 2013-02-14 Dolores Baksh Immune privileged and modulatory progenitor cells
US20080050814A1 (en) * 2006-06-05 2008-02-28 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of fetal placental cells
US20080064098A1 (en) * 2006-06-05 2008-03-13 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of maternal placental cells
US20090124007A1 (en) * 2006-11-15 2009-05-14 Seoul National University Industry Foundation Method for the Simultaneous Primary-Isolation and Expansion of Endothelial Stem/Progenitor Cell and Mesenchymal Stem Cell Derived From Mammal Including Human Umbilical Cord
US8673547B2 (en) * 2007-03-08 2014-03-18 Hemacell Perfusion, Inc. Method for isolation of afterbirth derived cells
WO2008153231A1 (en) * 2007-06-13 2008-12-18 Chabiotech Co., Ltd. Process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells
WO2009129616A1 (en) * 2008-04-21 2009-10-29 Tissue Regeneration Therapeutics, Inc. Genetically modified human umbilical cord perivascular cells for prophylaxis against or treatment of biological or chemical agents
KR101041914B1 (ko) * 2008-06-26 2011-06-15 부산대학교 산학협력단 셀레늄에 의해 탈분화된 세포, 이의 제조방법 및 이의 용도
EP2199383B1 (de) * 2008-12-22 2017-02-15 SpheroTec GmbH Verfahren zur Herstellung von Fibroblasten
ES2550975T3 (es) * 2010-03-30 2015-11-13 Histocell, S.L. Nuevo biomaterial procedente de gelatina de Wharton de cordón umbilical
US9677042B2 (en) 2010-10-08 2017-06-13 Terumo Bct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
WO2013079701A2 (en) 2011-11-30 2013-06-06 University Of Bremen Expression of mirnas in placental tissue
US8940294B2 (en) 2012-03-02 2015-01-27 Tissuetech, Inc. Methods of isolating and culturing stem cells
BR102013021202B1 (pt) * 2013-08-20 2022-09-06 Ccb - Centro De Criogenia Brasil Ltda Processo de produção de células tronco multipotentes e progenitores
US9617506B2 (en) 2013-11-16 2017-04-11 Terumo Bct, Inc. Expanding cells in a bioreactor
EP3613841B1 (de) 2014-03-25 2022-04-20 Terumo BCT, Inc. Passives ersetzen von medien
EP3198006B1 (de) 2014-09-26 2021-03-24 Terumo BCT, Inc. Geplante fütterung
CN104894088A (zh) * 2015-05-29 2015-09-09 广州赛莱拉干细胞科技股份有限公司 消化酶组合物及其应用、脐带上皮细胞的分离培养方法
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
EP3656842A1 (de) 2017-03-31 2020-05-27 Terumo BCT, Inc. Zellexpansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
CN115322949B (zh) * 2022-07-26 2024-03-15 唐颐控股(深圳)有限公司 一种人脐静脉平滑肌细胞的分离培养方法及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5134065A (en) 1986-05-16 1992-07-28 Monsanto Company Tissue plasminogen activator inhibitor and method of purification
US5919702A (en) 1996-10-23 1999-07-06 Advanced Tissue Science, Inc. Production of cartilage tissue using cells isolated from Wharton's jelly
EP2075015B1 (de) 1997-07-03 2015-03-11 Massachusetts Institute of Technology Gewebegezüchtete Konstrukte
WO1999054439A1 (en) 1998-04-17 1999-10-28 Science Research Laboratory, Inc. Isolation and purging of cells by means of osmotic pressure
JP2000139454A (ja) 1998-11-04 2000-05-23 Asahi Medical Co Ltd 細胞分離回収方法及び回収必要細胞含有液
AU4860900A (en) * 1999-06-02 2000-12-18 Lifebank Services, L.L.C. Methods of isolation, cryopreservation, and therapeutic use of human amniotic epithelial cells
US20020045260A1 (en) 2000-10-17 2002-04-18 Shih-Chieh Hung Method of isolating mesenchymal stem cells
US7560280B2 (en) 2000-11-03 2009-07-14 Kourion Therapeutics Gmbh Human cord blood derived unrestricted somatic stem cells (USSC)
KR100915482B1 (ko) 2000-12-06 2009-09-03 하리리 로버트 제이 태반 줄기 세포의 회수 방법
US6995162B2 (en) 2001-01-12 2006-02-07 Amgen Inc. Substituted alkylamine derivatives and methods of use
JP4212898B2 (ja) 2001-04-10 2009-01-21 バイオイー， インコーポレイテッド 細胞分離組成物および方法

Also Published As

Publication number Publication date
CA2529718A1 (en) 2005-01-06
EP1639099B1 (de) 2011-05-18
CA2529718C (en) 2012-10-23
US7939323B2 (en) 2011-05-10
WO2005001081A1 (en) 2005-01-06
EP1639099A1 (de) 2006-03-29
US20060199263A1 (en) 2006-09-07
US20080102522A1 (en) 2008-05-01
AU2003304250A1 (en) 2005-01-13

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