ATE471980T1 - Isolation von dna molekülen unter verwendung von arylmercapto-liganden - Google Patents
Isolation von dna molekülen unter verwendung von arylmercapto-ligandenInfo
- Publication number
- ATE471980T1 ATE471980T1 AT02730158T AT02730158T ATE471980T1 AT E471980 T1 ATE471980 T1 AT E471980T1 AT 02730158 T AT02730158 T AT 02730158T AT 02730158 T AT02730158 T AT 02730158T AT E471980 T1 ATE471980 T1 AT E471980T1
- Authority
- AT
- Austria
- Prior art keywords
- molecules
- isolation
- arylmercapto
- ligands
- dna molecules
- Prior art date
Links
- 238000002955 isolation Methods 0.000 title abstract 2
- 239000003446 ligand Substances 0.000 title 1
- 238000000034 method Methods 0.000 abstract 2
- 108091036055 CccDNA Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 238000001415 gene therapy Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Saccharide Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0101380A SE0101380D0 (sv) | 2001-04-18 | 2001-04-18 | Isolation of plasmid DNA molecules |
| SE0103436A SE0103436D0 (sv) | 2001-10-12 | 2001-10-12 | Isolation of plasmid DNA molecules |
| PCT/EP2002/004152 WO2002083893A2 (en) | 2001-04-18 | 2002-04-15 | Isolation of dna molecules using mercapto-aryl ligands |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE471980T1 true ATE471980T1 (de) | 2010-07-15 |
Family
ID=26655447
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02730158T ATE471980T1 (de) | 2001-04-18 | 2002-04-15 | Isolation von dna molekülen unter verwendung von arylmercapto-liganden |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6916919B2 (de) |
| EP (1) | EP1379645B1 (de) |
| JP (1) | JP4081376B2 (de) |
| AT (1) | ATE471980T1 (de) |
| AU (1) | AU2002302534B2 (de) |
| CA (1) | CA2444259C (de) |
| DE (2) | DE60236789D1 (de) |
| ES (1) | ES2347533T3 (de) |
| GB (1) | GB2377447B (de) |
| WO (1) | WO2002083893A2 (de) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0323814D0 (en) * | 2003-10-10 | 2003-11-12 | Cobra Biolog Ltd | Purification of biological macromolecules |
| KR100668338B1 (ko) | 2005-05-21 | 2007-01-12 | 삼성전자주식회사 | 신규한 ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는고체 기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법 |
| US20080300397A1 (en) * | 2007-05-31 | 2008-12-04 | Ge Healthcare Uk Limited | Modified spin column for simple and rapid plasmid dna extraction |
| WO2010046918A1 (en) * | 2008-10-08 | 2010-04-29 | V. B. Medicare Pvt. Ltd. | Purification of supercoiled plasmid dna |
| JP5463492B2 (ja) * | 2008-10-08 | 2014-04-09 | 島根県 | 微生物細胞からのプラスミドｄｎａ抽出法 |
| EP2598223B1 (de) | 2010-07-30 | 2022-04-06 | EMD Millipore Corporation | Chromatographiemedien und verfahren |
| CN103797053B (zh) | 2011-05-04 | 2017-06-20 | 康奈尔大学 | 多嵌段共聚物膜及其制备方法和用途 |
| CN106794389A (zh) | 2014-09-02 | 2017-05-31 | Emd密理博公司 | 具有纳米原纤化表面特征的高表面积纤维介质 |
| CN107001410A (zh) | 2014-12-08 | 2017-08-01 | Emd密理博公司 | 混合床离子交换吸附剂 |
| KR102308085B1 (ko) | 2016-04-28 | 2021-10-06 | 테라포어 테크놀로지스, 인코포레이티드 | 정전기적 분리를 위한 대전된 이소포러스 재료 |
| JP7104040B2 (ja) | 2016-11-17 | 2022-07-20 | ケー． シースジ，ジェイラジ | 高分子量の親水性添加剤を含有するイソポーラス自己集合ブロックコポリマーフィルム及びその製造方法 |
| US11567072B2 (en) | 2017-02-22 | 2023-01-31 | Terapore Technologies, Inc. | Ligand bound MBP membranes, uses and method of manufacturing |
| CA3062637A1 (en) | 2017-05-12 | 2018-11-15 | Terapore Technologies, Inc. | Chemically resistant fluorinated multiblock polymer structures, methods of manufacturing and use |
| CN111107924B (zh) | 2017-09-19 | 2022-12-27 | 特拉波雷技术有限公司 | 耐化学品的均孔交联嵌段共聚物结构 |
| WO2019178045A1 (en) | 2018-03-12 | 2019-09-19 | Terapore Technologies, Inc. | Isoporous mesoporous asymmetric block copolymer materials with macrovoids and method of making the same |
| CN109092263B (zh) * | 2018-08-28 | 2021-04-27 | 武汉瑞法医疗器械有限公司 | 一种低密度脂蛋白吸附剂及其制备方法 |
| CN109370963A (zh) * | 2018-09-21 | 2019-02-22 | 安徽欣伯玉生物科技有限公司 | 一种大肠杆菌工程菌高效表达超螺旋质粒dna的培养方法 |
| CN113969276B (zh) * | 2020-07-23 | 2024-12-13 | 苏州桑尼赛尔生物医药有限公司 | 一种高效纯化大量pcr产物的凝胶层析方法及其应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE470099B (sv) * | 1984-05-17 | 1993-11-08 | Jerker Porath | Sulfonaktiverade tioeteradsorbenter för separation av t ex protein |
| US5217593A (en) * | 1991-03-13 | 1993-06-08 | Macconnell William P | Nucleic acid purification system and method |
| US5502022A (en) * | 1994-05-16 | 1996-03-26 | Biosepra, Inc. | Chromatography adsorbents utilizing mercapto heterocyclic ligands |
| US5652348A (en) * | 1994-09-23 | 1997-07-29 | Massey University | Chromatographic resins and methods for using same |
| US5660984A (en) * | 1994-12-09 | 1997-08-26 | Davis; Thomas E. | DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof |
| US5789578A (en) * | 1996-01-11 | 1998-08-04 | Massey University | Methods for the preparation of resins with ligands attached thereto through a linking group comprising sulfide, sulfoxide or sulfone functionality |
| GB9927904D0 (en) * | 1999-11-25 | 2000-01-26 | Amersham Pharm Biotech Ab | A method fro obtaining a nucleic acid variant |
| EP1683631A1 (de) * | 2005-01-25 | 2006-07-26 | Borealis Technology OY | Mehrschichtstrukturen |
-
2002
- 2002-04-12 GB GB0208591A patent/GB2377447B/en not_active Expired - Lifetime
- 2002-04-15 DE DE60236789T patent/DE60236789D1/de not_active Expired - Lifetime
- 2002-04-15 JP JP2002582231A patent/JP4081376B2/ja not_active Expired - Lifetime
- 2002-04-15 EP EP02730158A patent/EP1379645B1/de not_active Expired - Lifetime
- 2002-04-15 US US10/473,239 patent/US6916919B2/en not_active Expired - Lifetime
- 2002-04-15 CA CA2444259A patent/CA2444259C/en not_active Expired - Fee Related
- 2002-04-15 AT AT02730158T patent/ATE471980T1/de not_active IP Right Cessation
- 2002-04-15 AU AU2002302534A patent/AU2002302534B2/en not_active Ceased
- 2002-04-15 WO PCT/EP2002/004152 patent/WO2002083893A2/en not_active Ceased
- 2002-04-15 ES ES02730158T patent/ES2347533T3/es not_active Expired - Lifetime
- 2002-04-17 DE DE10217084A patent/DE10217084A1/de not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JP2004533237A (ja) | 2004-11-04 |
| US6916919B2 (en) | 2005-07-12 |
| JP4081376B2 (ja) | 2008-04-23 |
| CA2444259C (en) | 2012-10-02 |
| US20040126778A1 (en) | 2004-07-01 |
| GB2377447B (en) | 2003-06-25 |
| CA2444259A1 (en) | 2002-10-24 |
| DE60236789D1 (de) | 2010-08-05 |
| EP1379645A2 (de) | 2004-01-14 |
| GB0208591D0 (en) | 2002-05-22 |
| ES2347533T3 (es) | 2010-11-02 |
| DE10217084A1 (de) | 2002-11-21 |
| WO2002083893A3 (en) | 2003-07-31 |
| WO2002083893A2 (en) | 2002-10-24 |
| GB2377447A (en) | 2003-01-15 |
| EP1379645B1 (de) | 2010-06-23 |
| AU2002302534B2 (en) | 2007-09-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |