ATE456671T1 - Nachweis von zellulärem stress mittels hmox-1 promoter - Google Patents

Nachweis von zellulärem stress mittels hmox-1 promoter

Info

Publication number
ATE456671T1
ATE456671T1 AT05759714T AT05759714T ATE456671T1 AT E456671 T1 ATE456671 T1 AT E456671T1 AT 05759714 T AT05759714 T AT 05759714T AT 05759714 T AT05759714 T AT 05759714T AT E456671 T1 ATE456671 T1 AT E456671T1
Authority
AT
Austria
Prior art keywords
constructs
nucleic acid
acid sequences
reporter gene
promoter
Prior art date
Application number
AT05759714T
Other languages
English (en)
Inventor
Charles Roland Wolf
Christopher B A Whitelaw
Clifford Roy Elcombe
Kenneth Brown
Anthony Clark
Original Assignee
Cxr Biosciences Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cxr Biosciences Ltd filed Critical Cxr Biosciences Ltd
Application granted granted Critical
Publication of ATE456671T1 publication Critical patent/ATE456671T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Eye Examination Apparatus (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT05759714T 2004-07-16 2005-07-15 Nachweis von zellulärem stress mittels hmox-1 promoter ATE456671T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0415963.8A GB0415963D0 (en) 2004-07-16 2004-07-16 Detection of cellular stress
PCT/GB2005/002779 WO2006008484A2 (en) 2004-07-16 2005-07-15 Detection of cellular stress

Publications (1)

Publication Number Publication Date
ATE456671T1 true ATE456671T1 (de) 2010-02-15

Family

ID=32893703

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05759714T ATE456671T1 (de) 2004-07-16 2005-07-15 Nachweis von zellulärem stress mittels hmox-1 promoter

Country Status (9)

Country Link
US (1) US20100138935A1 (de)
EP (1) EP1769085B1 (de)
JP (1) JP2008506380A (de)
AT (1) ATE456671T1 (de)
AU (1) AU2005263930B2 (de)
CA (1) CA2574179A1 (de)
DE (1) DE602005019165D1 (de)
GB (2) GB0415963D0 (de)
WO (1) WO2006008484A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007002568A1 (en) * 2005-06-22 2007-01-04 Geron Corporation Reporter hepatocytes and other cells for drug screening and toxicity testing
CA2643414A1 (en) * 2006-03-10 2007-09-20 Ziad Anwar Ali A method for stenting a vessel of a rodent and for facilitating monitoring of the effect of stenting a vessel of a rodent
CN101319215B (zh) * 2008-07-01 2010-12-22 郑骏年 人肿瘤特异性Ki67基因启动子
WO2012022723A1 (en) * 2010-08-16 2012-02-23 F. Hoffmann-La Roche Ag Reporter mouse for delivery of rnai
WO2012035120A2 (en) * 2010-09-15 2012-03-22 Universiteit Leiden Screening method
JP5804629B2 (ja) * 2011-07-29 2015-11-04 株式会社メディクローム 遺伝子発現変動解析による化学物質の毒性評価方法
JPWO2014115571A1 (ja) * 2013-01-28 2017-01-26 桂子 池本 Ｄ−細胞の可視化方法
WO2020174539A1 (ja) * 2019-02-25 2020-09-03 国立大学法人東北大学 細胞増殖モニター用非ヒト哺乳動物
EP3991573A1 (de) 2020-11-03 2022-05-04 Patrick Faber Zusammensetzungen zur nasalen applikation

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69316368T2 (de) * 1992-07-06 1998-06-25 Harvard College Verfahren und diagnostische kits zur bestimmung der toxizität einer verbindung unter verwendung von an bakterielle stresspromotoren fusionierten reportergenen
AU722375B2 (en) * 1996-09-06 2000-08-03 Trustees Of The University Of Pennsylvania, The Methods using cre-lox for production of recombinant adeno-associated viruses
US6632980B1 (en) * 1997-10-24 2003-10-14 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
EP0950717A1 (de) * 1998-04-14 1999-10-20 "VLAAMSE INSTELLING VOOR TECHNOLOGISCH ONDERZOEK", afgekort "V.I.T.O." Diagnostisches System und Verfahren zur Bestimmung genotoxischer Substanzen in einer Probe
US6723896B1 (en) * 1999-11-12 2004-04-20 The Rockefeller University Inducible site-specific recombination for the activation and removal of transgenes in transgenic plants
US20020015940A1 (en) * 2000-03-29 2002-02-07 Govind Rao Method of gene expression screening
US20020150556A1 (en) * 2000-03-31 2002-10-17 Vile Richard G. Compositions and methods for tissue specific gene regulation therapy
WO2002006451A1 (en) * 2000-07-18 2002-01-24 Uab Research Foundation Tissue-specific self-inactivating gene therapy vector
JP2003079365A (ja) * 2001-09-07 2003-03-18 Kanegafuchi Chem Ind Co Ltd 環境有害化学物質の高感度検出法
GB0217402D0 (en) * 2002-07-26 2002-09-04 Roslin Inst Edinburgh Multi-reporter gene model for toxicological screening

Also Published As

Publication number Publication date
AU2005263930A1 (en) 2006-01-26
GB0514518D0 (en) 2005-08-24
EP1769085B1 (de) 2010-01-27
GB2416771A (en) 2006-02-08
CA2574179A1 (en) 2006-01-26
JP2008506380A (ja) 2008-03-06
AU2005263930A2 (en) 2006-01-26
US20100138935A1 (en) 2010-06-03
WO2006008484A2 (en) 2006-01-26
GB0415963D0 (en) 2004-08-18
WO2006008484A3 (en) 2006-09-14
EP1769085A2 (de) 2007-04-04
AU2005263930B2 (en) 2011-04-07
GB2416771B (en) 2009-05-27
DE602005019165D1 (de) 2010-03-18

Similar Documents

Publication Publication Date Title
ATE456671T1 (de) Nachweis von zellulärem stress mittels hmox-1 promoter
Mayor et al. End of century ocean warming and acidification effects on reproductive success in a temperate marine copepod
Bianco et al. Expanding the genetic code of Drosophila melanogaster
Verhaegen et al. Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization
Angilletta Jr Looking for answers to questions about heat stress: researchers are getting warmer
MX354857B (es) Metodo para la expresion transitoria de acidos nucleicos en las plantas.
CY1119031T1 (el) Βακτηριακως λαμβανομενα, ανεπαφα μινικυτταρα που συμπεριλαμβανουν ρυθμιστικο rna
ATE490321T1 (de) Induzierbare sirna expressionskonstrukte zur gezielten genabschaltung
DE602005021493D1 (de) Zellcyclusphasenmarker
CN104232588A (zh) 一种抗肝纤维化药物高通量筛选细胞模型的构建及应用
Konishi et al. Characterization of vacuolar membrane proteins changed in rice root treated with gibberellin
Hellweger 100 Years since Streeter and Phelps: it is time to update the biology in our water quality models
Majerová et al. A BI-1 mediated cascade improves redox homeostasis during thermal stress and prevents oxidative damage in a preconditioned reef-building coral
ATE540313T1 (de) Identifizierung von keimbahnfähigen embryonischen stammzellen
Hosseinkhani et al. Luciferin‐regenerating enzyme crystal structure is solved but its function is still unclear
Tabunoki et al. BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori
Luhur et al. Starving for more: Nutrient sensing by LIN-28 in adult intestinal progenitor cells
Tian et al. Genomewide analysis of sperm whale E2 ubiquitin conjugating enzyme genes
Wan et al. Role of the repressor Oaf3p in the recruitment of transcription factors and chromatin dynamics during the oleate response
Dai Chromatin regulators and transcriptional control of Drosophila development
Loots et al. Interrogating transcriptional regulatory sequences in Tol2-mediated Xenopus transgenics
Caiyun et al. A Nano Luciferase toolkit for studying plant disease signaling pathways
Larashati et al. Inhibition of Reporter Genes by Small Interfering RNAs in Cell Culture and Living Fish
Chetverina et al. SV40 transcription terminators stabilize the activity of regulatory elements in Drosophila melanogaster
Masson et al. Identification and characterization of a Metazoan Proteasome Regulator 11S REG (PA28) in Dictyostelium Discoideum

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties