ATE448325T1 - Asynchrones pcr priming - Google Patents

Asynchrones pcr priming

Info

Publication number
ATE448325T1
ATE448325T1 AT01946152T AT01946152T ATE448325T1 AT E448325 T1 ATE448325 T1 AT E448325T1 AT 01946152 T AT01946152 T AT 01946152T AT 01946152 T AT01946152 T AT 01946152T AT E448325 T1 ATE448325 T1 AT E448325T1
Authority
AT
Austria
Prior art keywords
probe
reporter dye
asynchronous
stranded
annealed
Prior art date
Application number
AT01946152T
Other languages
English (en)
Inventor
Caifu Chen
Michael Egholm
Lawrence Haff
Original Assignee
Applied Biosystems Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Biosystems Llc filed Critical Applied Biosystems Llc
Application granted granted Critical
Publication of ATE448325T1 publication Critical patent/ATE448325T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT01946152T 2000-06-06 2001-06-06 Asynchrones pcr priming ATE448325T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20988300P 2000-06-06 2000-06-06
US09/875,211 US6887664B2 (en) 2000-06-06 2001-06-05 Asynchronous primed PCR
PCT/US2001/018464 WO2001094638A2 (en) 2000-06-06 2001-06-06 Asynchronous primed pcr

Publications (1)

Publication Number Publication Date
ATE448325T1 true ATE448325T1 (de) 2009-11-15

Family

ID=26904613

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01946152T ATE448325T1 (de) 2000-06-06 2001-06-06 Asynchrones pcr priming

Country Status (8)

Country Link
US (2) US6887664B2 (de)
EP (3) EP2256213A1 (de)
JP (1) JP2004509613A (de)
AT (1) ATE448325T1 (de)
AU (1) AU6823501A (de)
CA (1) CA2412413A1 (de)
DE (1) DE60140487D1 (de)
WO (1) WO2001094638A2 (de)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6887664B2 (en) * 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
CN100523216C (zh) * 2001-03-02 2009-08-05 匹兹堡大学 Pcr方法
US20030175749A1 (en) 2001-12-08 2003-09-18 Jong-Yoon Chun Annealing control primer and its uses
EP1942196B1 (de) 2001-12-19 2012-05-30 Brandeis University Late-PCR
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP2003199568A (ja) * 2002-01-10 2003-07-15 Nichirei Corp Ｄｎａ増幅反応の効率向上方法
US7582429B2 (en) 2002-10-23 2009-09-01 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
JP4280519B2 (ja) * 2003-03-07 2009-06-17 アークレイ株式会社 Ｄｎａ増幅法およびそのためのキット
JP4322554B2 (ja) * 2003-05-19 2009-09-02 株式会社ニチレイフーズ Ｄｎａ増幅反応の効率向上方法
US20040235032A1 (en) * 2003-05-19 2004-11-25 Canon Kabushiki Kaisha PCR amplification method, PCR primer set, PCR amplification product, and method for detection of nucleic acid using the amplification method
JP2005000162A (ja) * 2003-05-19 2005-01-06 Canon Inc 核酸のｐｃｒ増幅方法、ｐｃｒプライマー・セット、ｐｃｒ増幅産物、ならびに、該増幅方法を利用する核酸の検出方法
US8058032B2 (en) 2003-11-10 2011-11-15 Seegene, Inc. Method for amplifying unknown DNA sequence adjacent to known sequence
ES2432040T3 (es) * 2004-01-28 2013-11-29 454 Life Sciences Corporation Amplificación de ácido nucleico con emulsión de flujo continuo
US7481997B1 (en) 2004-02-12 2009-01-27 Montana State University Snow mountain virus genome sequence, virus-like particles and methods of use
US7387887B2 (en) * 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
EP1836319B1 (de) 2005-01-06 2012-09-19 Life Technologies Corporation Polypeptide mit nukleinsäurebindender aktivität sowie verfahren zur nukleinsäureamplifikation
CN101171343A (zh) 2005-04-14 2008-04-30 阿普里拉股份有限公司 含有假异胞嘧啶核碱基衍生物的3’修饰寡核苷酸及其作为引物或探针的应用
EP1880021A2 (de) * 2005-05-02 2008-01-23 Stratagene California Oligonukleotidsonde/primerzusammensetzungen und verfahren zum nachweis von oligonukleotiden
AU2006341607B2 (en) * 2005-05-31 2011-03-17 Applied Biosystems, Llc. Multiplexed amplification of short nucleic acids
EP1904079A4 (de) * 2005-06-02 2009-10-21 Advandx Inc Peptid-nukleinsäure-sonden zur untersuchung von mikroorganismen
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US20070212704A1 (en) 2005-10-03 2007-09-13 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
CA2647566C (en) 2006-03-29 2017-01-03 Merial Limited Vaccine against streptococci
US10829803B2 (en) 2006-05-10 2020-11-10 Dxterity Diagnostics Incorporated Detection of nucleic acid targets using chemically reactive oligonucleotide probes
AU2007275762B2 (en) 2006-07-17 2012-02-23 Brandeis University Specialized oligonucleotides and their use in nucleic acid amplification and detection
WO2008072933A1 (en) * 2006-12-15 2008-06-19 Panagene Inc. Peptide nucleic acids conjugated with multi -amine linkers and nucleic acid detecting device using the same
KR101072900B1 (ko) * 2007-12-04 2011-10-17 주식회사 파나진 고정화된 펩티드핵산 프로브를 사용한 표적핵산의 선택적 표지 및 검출 방법
FI121379B (fi) * 2008-01-17 2010-10-29 Mobidiag Oy Menetelmä yksijuosteisen DNA:n tuottamiseksi kaksivaiheisella symmetrisellä PCR:llä sekä menetelmään tarkoitettu reagenssipakkaus
GB0806041D0 (en) 2008-04-03 2008-05-14 Genomica S A Method for detection of herpesvirus in test sample
US8993230B2 (en) * 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
US9976177B2 (en) 2009-04-01 2018-05-22 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (CLPA)
US9334531B2 (en) * 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US20110039304A1 (en) * 2009-08-12 2011-02-17 President And Fellows Of Harvard College Methods to Generate Oligonucleotide Pools and Enrich Target Nucleic Acid Sequences
US9127309B2 (en) * 2011-01-31 2015-09-08 Qiagen Mansfield, Inc. Methods of nucleic acid quantification and detection using anomalous migration
WO2012158967A1 (en) 2011-05-17 2012-11-22 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
EP2722399A1 (de) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Verfahren zur Vermeidung von Produkten mit hohem Molekulargewicht während einer Amplifikation
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
WO2015191777A2 (en) 2014-06-10 2015-12-17 Dxterity Diagnostics Incorporated Devices and methods for collecting and stabilizing biological samples
US20170233818A1 (en) * 2014-07-25 2017-08-17 Panagene Inc. Multiple target nucleic acid detection method using clamping probe and detection probe
PL3298162T3 (pl) * 2015-05-18 2020-07-27 Saga Diagnostics Ab Wykrywanie docelowego kwasu nukleinowego i wariantów
CN109642253A (zh) 2016-08-02 2019-04-16 豪夫迈·罗氏有限公司 用于提高核酸的扩增和检测/定量的效率的辅助寡核苷酸
CN108342489A (zh) * 2017-01-23 2018-07-31 公安部物证鉴定中心 一种对男性个体进行y-snp分型的方法和系统
US10513730B2 (en) * 2017-11-22 2019-12-24 Safeguard Biosystems Holdings Ltd. Asymmetric PCR methods, primers and kits
KR101919896B1 (ko) * 2018-05-03 2018-11-19 주식회사 유디피아 단일 형광 채널 유전자증폭기기를 이용한 다중 핵산 검출 방법
EP3844303A4 (de) * 2018-08-27 2022-06-01 The Regents of The University of California Reporternukleinsäuren für typ-v-crispr-vermittelte detektion
KR20210026867A (ko) * 2019-09-02 2021-03-10 주식회사 시선바이오머티리얼스 신규한 pna 올리고머, 이의 dna 메틸화 검출용도 및 이를 이용한 dna 메틸화 검출방법
EP4087941A4 (de) * 2020-01-10 2023-06-28 Siemens Healthcare Diagnostics, Inc. Zusammensetzungen, kits und verfahren zum durchführen von schnellen polymerase-kettenreaktionen

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4973679A (en) 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US4931223A (en) 1986-07-24 1990-06-05 Tropix, Inc. Methods of using chemiluminescent 1,2-dioxetanes
JPH02504672A (ja) 1986-12-15 1990-12-27 ブリティッシュ　テクノロジー　グループ　ユーエスエイ，インコーポレイテッド 単量体性フタロシアニン試薬
US5231191A (en) 1987-12-24 1993-07-27 Applied Biosystems, Inc. Rhodamine phosphoramidite compounds
US5262530A (en) 1988-12-21 1993-11-16 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US5047524A (en) 1988-12-21 1991-09-10 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US5141813A (en) 1989-08-28 1992-08-25 Clontech Laboratories, Inc. Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5654442A (en) 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
DK0519338T3 (da) * 1991-06-20 1996-10-28 Hoffmann La Roche Forbedrede fremgangsmåder til nukleinsyreamplifikation
US5470705A (en) 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
DE69322266T2 (de) 1992-04-03 1999-06-02 Perkin-Elmer Corp., Foster City, Calif. Proben zusammensetzung und verfahren
CA2129787A1 (en) 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
EP0760008A1 (de) 1994-05-19 1997-03-05 Dako A/S Pna-sonden zum nachweis von neisseria gonorrhoeae und chlamydia trachomatis
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US5972693A (en) 1995-10-24 1999-10-26 Curagen Corporation Apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
US5736626A (en) 1996-01-29 1998-04-07 The Perkin-Elmer Corporation Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
US6020481A (en) 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US5847162A (en) 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
SE506700C2 (sv) 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
US5821356A (en) 1996-08-12 1998-10-13 The Perkin Elmer Corporation Propargylethoxyamino nucleotides
DE19637339A1 (de) 1996-09-13 1998-03-19 Hoechst Ag Verfahren zur Amplifikation von Nukleinsäuren
ATE232880T1 (de) 1996-11-18 2003-03-15 Takeshi Imanishi Neue nucleotidanaloga
US6110676A (en) 1996-12-04 2000-08-29 Boston Probes, Inc. Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
US5770716A (en) 1997-04-10 1998-06-23 The Perkin-Elmer Corporation Substituted propargylethoxyamido nucleosides, oligonucleotides and methods for using same
EP2341058A3 (de) 1997-09-12 2011-11-23 Exiqon A/S Oligonukleotidanaloga
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
AU1366299A (en) 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US5936087A (en) 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
ATE271422T1 (de) 1998-05-01 2004-08-15 Hoffmann La Roche Vorrichtung zur gleichzeitigen überwachung von reaktionen, die in einer vielzahl von reaktionsgefässen stattfinden
US6117986A (en) 1998-06-10 2000-09-12 Intergen Company, L.P. Pyrimidines linked to a quencher
US6037130A (en) 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US6887664B2 (en) * 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR

Also Published As

Publication number Publication date
CA2412413A1 (en) 2001-12-13
EP2256213A1 (de) 2010-12-01
US20030207266A1 (en) 2003-11-06
WO2001094638A3 (en) 2003-07-10
US20050130178A1 (en) 2005-06-16
AU6823501A (en) 2001-12-17
DE60140487D1 (en) 2009-12-24
EP1368489B1 (de) 2009-11-11
US6887664B2 (en) 2005-05-03
WO2001094638A8 (en) 2002-04-11
EP2316975A1 (de) 2011-05-04
JP2004509613A (ja) 2004-04-02
EP1368489A2 (de) 2003-12-10
WO2001094638A2 (en) 2001-12-13

Similar Documents

Publication Publication Date Title
DE60140487D1 (en) Asynchrones pcr priming
CA2532160A1 (en) Methods of genotyping using differences in melting temperature
CA2511128A1 (en) Late-pcr
JP6166342B2 (ja) ホスホロチオエート基を含むオリゴヌクレオチドを用いる、ポリメラーゼ連鎖反応による検出システム
EP1716257B8 (de) Neue primer und sonden zum nachweis von parvovirus b19
DE60031489D1 (de) Universalproben und Methoden zum Nachweis von Nukleinsäuren
CA2255531A1 (en) Labeled primer for use in and detection of target nucleic acids
CN104487596B (zh) 用于聚合酶链式反应(pcr)的新型组合物、方法和试剂盒
JP2009511056A5 (de)
CA2516299A1 (en) Compositions and methods for multiplex analysis of polynucleotides
DE60108897D1 (de) Methoden für externe kontrollen zur nukleinsäure amplifizierung
WO2007127992A3 (en) Use of base-modified deoxynucleoside triphosphates
ES2776427T3 (es) Método de amplificación de ADN basado en invasión de cadena
KR20120018734A (ko) 헤파티티스 ｂ 바이러스 검출용 키트 및 이를 이용한 헤파티티스 ｂ 바이러스의 검출 방법
DE60309817D1 (de) Dopplezweck -Primer und -Proben für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen
US8198026B2 (en) Method of detecting specific fragments of DNA or RNA with the aid of a real-time polymerase chain reaction
JP2013515458A5 (de)
EP2373808B1 (de) Verbesserte amplifikation auf taqman-sondenbasis
ATE480639T1 (de) Neues format zur detektion von heissstart- und echtzeit-polymerasekettenreaktion
KR20140123858A (ko) 폴리뉴클레오티드 및 그의 용도
DE60325679D1 (de) Verbesserte Koamplifikation von Nukleinsäuren
CN106868111A (zh) 利用通用TaqMan探针检测SNP的方法和试剂盒
JP2003506068A5 (de)
CN108026569B (zh) 用于催化性测定的方法和组合物
Jothikumar et al. A novel photoinduced electron transfer (PET) primer technique for rapid real-time PCR detection of Cryptosporidium spp.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties