DE60325679D1 - Verbesserte Koamplifikation von Nukleinsäuren - Google Patents

Verbesserte Koamplifikation von Nukleinsäuren

Info

Publication number
DE60325679D1
DE60325679D1 DE60325679T DE60325679T DE60325679D1 DE 60325679 D1 DE60325679 D1 DE 60325679D1 DE 60325679 T DE60325679 T DE 60325679T DE 60325679 T DE60325679 T DE 60325679T DE 60325679 D1 DE60325679 D1 DE 60325679D1
Authority
DE
Germany
Prior art keywords
target nucleic
primer extension
nucleic acids
reaction mixture
temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60325679T
Other languages
English (en)
Inventor
Holger Engel
Dirk Loeffert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Application granted granted Critical
Publication of DE60325679D1 publication Critical patent/DE60325679D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE60325679T 2002-09-24 2003-09-23 Verbesserte Koamplifikation von Nukleinsäuren Expired - Lifetime DE60325679D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP20020021174 EP1403379A1 (de) 2002-09-24 2002-09-24 Verbesserte Koamplifikation von Nukleinsäuren

Publications (1)

Publication Number Publication Date
DE60325679D1 true DE60325679D1 (de) 2009-02-26

Family

ID=31970287

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60325679T Expired - Lifetime DE60325679D1 (de) 2002-09-24 2003-09-23 Verbesserte Koamplifikation von Nukleinsäuren

Country Status (5)

Country Link
US (1) US8067206B2 (de)
EP (1) EP1403379A1 (de)
JP (1) JP4675558B2 (de)
AT (1) ATE420208T1 (de)
DE (1) DE60325679D1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
AU2005250233B2 (en) * 2004-06-01 2011-03-17 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
GB2424886A (en) * 2005-04-04 2006-10-11 Dxs Ltd Polynucleotide primers against epidermal growth factor receptor and method of detecting gene mutations
EP2829615B1 (de) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit zur Multiplexierung einer Rekombinase-Polymerase-Verstärkung
MY143596A (en) 2005-08-11 2011-06-15 Synthetic Genomics Inc In vitro recombination method
CA2618665C (en) * 2005-08-11 2012-11-13 J. Craig Venter Institute Method for in vitro recombination
AU2007298650B2 (en) 2006-05-04 2013-10-17 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
EP2077337A1 (de) * 2007-12-26 2009-07-08 Eppendorf Array Technologies SA Zusammensetzung, Verfahren und Kit zum Nachweis und zur Amplifikation
US9469867B2 (en) 2009-05-20 2016-10-18 Alere San Diego, Inc. DNA glycosylase/lyase and AP endonuclease substrates
EP2369325A1 (de) * 2010-03-12 2011-09-28 Eppendorf Ag Arrayanalyse für Online-Detektion
US9587268B2 (en) * 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2555589B1 (fr) * 1983-11-30 1986-05-16 Choay Sa Nouveaux derives du dextrane a activites anticoagulante en anti-inflammatoire, leur procede de preparation et utilisation de ces derives en tant qu'anticoagulants et en tant que substituts du plasma sanguin
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4902624A (en) * 1987-11-23 1990-02-20 Eastman Kodak Company Temperature cycling cuvette
US5459038A (en) * 1988-01-29 1995-10-17 Advanced Riverina Holdings, Ltd. Determination of genetic sex in ruminants using Y-chromosome specific polynucleotides
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5229297A (en) * 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
US5089233A (en) 1989-06-12 1992-02-18 Eastman Kodak Company Processing apparatus for a chemical reaction pack
US5106730A (en) * 1989-07-24 1992-04-21 Microprobe Corporation Lactam-containing compositions and methods useful for the hybridization of nucleic acids
ES2141088T3 (es) * 1990-02-16 2000-03-16 Hoffmann La Roche Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa.
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US5173260A (en) * 1990-09-17 1992-12-22 Eastman Kodak Company Beads fused to a test device support
US5705366A (en) * 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6183998B1 (en) * 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
EP1111044A4 (de) * 1998-09-08 2003-06-04 Takara Shuzo Co Methode zur dna-synthese
JP4186269B2 (ja) * 1998-09-21 2008-11-26 株式会社島津製作所 核酸合成法
EP0989192A3 (de) * 1998-09-21 2004-02-25 Shimadzu Corporation Verfahren zur Nucleinsäuresynthese

Also Published As

Publication number Publication date
JP4675558B2 (ja) 2011-04-27
JP2004113242A (ja) 2004-04-15
US20040115712A1 (en) 2004-06-17
ATE420208T1 (de) 2009-01-15
EP1403379A1 (de) 2004-03-31
US8067206B2 (en) 2011-11-29

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