ATE430805T1 - Modulation der polypeptidexpression auf modifizierten filamentösen phagen - Google Patents

Modulation der polypeptidexpression auf modifizierten filamentösen phagen

Info

Publication number
ATE430805T1
ATE430805T1 AT99942093T AT99942093T ATE430805T1 AT E430805 T1 ATE430805 T1 AT E430805T1 AT 99942093 T AT99942093 T AT 99942093T AT 99942093 T AT99942093 T AT 99942093T AT E430805 T1 ATE430805 T1 AT E430805T1
Authority
AT
Austria
Prior art keywords
modulation
polypeptide expression
filamentous phages
modified filamentous
phage
Prior art date
Application number
AT99942093T
Other languages
English (en)
Inventor
Angray Kang
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Application granted granted Critical
Publication of ATE430805T1 publication Critical patent/ATE430805T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Endocrinology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT99942093T 1998-08-12 1999-08-11 Modulation der polypeptidexpression auf modifizierten filamentösen phagen ATE430805T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9632698P 1998-08-12 1998-08-12
US09/198,839 US6190908B1 (en) 1998-08-12 1998-12-24 Modulation of polypeptide display on modified filamentous phage
PCT/US1999/018207 WO2000009715A1 (en) 1998-08-12 1999-08-11 Modulation of polypeptide display on modified filamentous phage

Publications (1)

Publication Number Publication Date
ATE430805T1 true ATE430805T1 (de) 2009-05-15

Family

ID=26791575

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99942093T ATE430805T1 (de) 1998-08-12 1999-08-11 Modulation der polypeptidexpression auf modifizierten filamentösen phagen

Country Status (8)

Country Link
US (3) US6190908B1 (de)
EP (1) EP1127142B1 (de)
JP (1) JP2002522084A (de)
AT (1) ATE430805T1 (de)
AU (1) AU766253B2 (de)
CA (1) CA2336541C (de)
DE (1) DE69940853D1 (de)
WO (1) WO2000009715A1 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6589730B1 (en) 1997-08-29 2003-07-08 Selective Genetics, Inc. Methods for identifying protein-protein interactions by selective transduction
US6054312A (en) * 1997-08-29 2000-04-25 Selective Genetics, Inc. Receptor-mediated gene delivery using bacteriophage vectors
US20030148263A1 (en) * 1997-08-29 2003-08-07 Selective Genetics, Inc. Methods and compositions using genetic package display
US6723512B2 (en) 1997-08-29 2004-04-20 Selective Genetics Inc. Methods using genetic package display for detecting and identifying protein-protein interactions that facilitate internalization and transgene expression and cells or tissues competent for the same and methods for evolving gene delivery vectors
NZ520579A (en) * 1997-10-24 2004-08-27 Invitrogen Corp Recombinational cloning using nucleic acids having recombination sites and methods for synthesizing double stranded nucleic acids
EP1060395B1 (de) 1998-02-04 2008-04-30 Invitrogen Corporation Microarrays und ihre verwendungen
AU770540B2 (en) * 1998-04-30 2004-02-26 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Novel method for the identification of clones conferring a desired biological property from an expression library
US6406921B1 (en) * 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6780582B1 (en) * 1998-07-14 2004-08-24 Zyomyx, Inc. Arrays of protein-capture agents and methods of use thereof
US20030138973A1 (en) * 1998-07-14 2003-07-24 Peter Wagner Microdevices for screening biomolecules
US6576478B1 (en) * 1998-07-14 2003-06-10 Zyomyx, Inc. Microdevices for high-throughput screening of biomolecules
US20020119579A1 (en) * 1998-07-14 2002-08-29 Peter Wagner Arrays devices and methods of use thereof
AU5122499A (en) 1998-07-27 2000-02-21 Genentech Inc. Improved transformation efficiency in phage display through modification of a coat protein
US6190908B1 (en) * 1998-08-12 2001-02-20 The Scripps Research Institute Modulation of polypeptide display on modified filamentous phage
ES2260009T3 (es) 1999-05-05 2006-11-01 Phylogica Limited Aislamiento de moduladores biologicos a partir de bancos de fragmentos genicos biodiversos.
US7270969B2 (en) * 1999-05-05 2007-09-18 Phylogica Limited Methods of constructing and screening diverse expression libraries
US7803765B2 (en) * 1999-05-05 2010-09-28 Phylogica Limited Methods of constructing biodiverse gene fragment libraries and biological modulators isolated therefrom
EP1283909A4 (de) * 2000-05-04 2006-06-07 Univ Yale Proteinarrays mit hoher dichte zum screenen von proteinaktivität
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
AU2002225914A1 (en) * 2000-12-08 2002-06-18 Alexion Pharmaceuticals, Inc. Plasmid vectors
EP1493028A4 (de) * 2001-07-06 2006-06-14 Genentech Inc Im phagen-display präsentierte liganden der pdz-domäne
WO2003008437A2 (en) 2001-07-20 2003-01-30 California Institute Of Technology Protein and nucleic acid expression systems
WO2003097796A2 (en) * 2002-05-14 2003-11-27 Alexion Pharmaceuticals, Inc. T7 bacteriophage display of fabs
WO2004013290A2 (en) * 2002-08-05 2004-02-12 Invitrogen Corporation Compositions and methods for molecular biology
US20050233473A1 (en) * 2002-08-16 2005-10-20 Zyomyx, Inc. Methods and reagents for surface functionalization
AU2003302470A1 (en) * 2002-11-26 2004-06-18 Dyax Corporation Methods and compositions for controlling valency of phage display
DE602004031589D1 (de) * 2003-01-07 2011-04-14 Dyax Corp Kunitz-domäne-bibliothek
US20040248323A1 (en) * 2003-06-09 2004-12-09 Protometrix, Inc. Methods for conducting assays for enzyme activity on protein microarrays
US20050106667A1 (en) * 2003-08-01 2005-05-19 Genentech, Inc Binding polypeptides with restricted diversity sequences
JP2007501011A (ja) * 2003-08-01 2007-01-25 ジェネンテック・インコーポレーテッド 制限多様性配列を有する結合型ポリペプチド
US20050048506A1 (en) * 2003-09-03 2005-03-03 Fredrick Joseph P. Methods for encoding non-biological information on microarrays
US7108979B2 (en) * 2003-09-03 2006-09-19 Agilent Technologies, Inc. Methods to detect cross-contamination between samples contacted with a multi-array substrate
US20050049796A1 (en) * 2003-09-03 2005-03-03 Webb Peter G. Methods for encoding non-biological information on microarrays
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2005118647A2 (en) * 2004-05-18 2005-12-15 Genentech, Inc. M13 virus major coat protein variants for c-terminal and bi-terminal display of a heterologous protein
CA2568644C (en) * 2004-06-03 2015-11-24 Phylogica Limited Peptide modulators of cellular phenotype and bi-nucleic acid fragment library
WO2007001302A1 (en) * 2005-06-28 2007-01-04 The Scripps Research Institute Delivery of active proteins to the central nervous system using phage vectors
WO2007056441A2 (en) 2005-11-07 2007-05-18 Genentech, Inc. Binding polypeptides with diversified and consensus vh/vl hypervariable sequences
EP1973951A2 (de) * 2005-12-02 2008-10-01 Genentech, Inc. Bindende polypeptide mit eingeschränkten diversitätssequenzen
DK1987178T3 (en) 2006-02-20 2015-04-20 Phylogica Ltd Process for the construction and screening of peptide structure libraries
CA2663545A1 (en) * 2006-09-19 2008-03-27 Phylogica Limited Neuroprotective peptide inhibitors of ap-1 signaling and uses thereof
WO2008154700A1 (en) * 2007-06-20 2008-12-24 Phylogica Limited Compositions and uses thereof for the treatment of acute respiratory distress syndrome (ards) and clinical disorders associated with therewith
MX2010004807A (es) * 2007-11-02 2010-08-02 Scripss Res Inst Evolucion dirigida utilizando proteinas que comprenden aminoacidos no naturales.
EP3103810A3 (de) 2011-04-21 2017-03-08 Garvan Institute of Medical Research Modifizierte moleküle mit variablen domänen sowie herstellungs- und verwendungsverfahren dafür
EP3209697A4 (de) 2014-10-23 2018-05-30 La Trobe University Fn14-bindende proteine und verwendungen davon
DK3242685T3 (da) 2015-01-09 2022-07-25 Adalta Ltd Cxcr4-bindende molekyler

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
IL99552A0 (en) * 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
US5698426A (en) * 1990-09-28 1997-12-16 Ixsys, Incorporated Surface expression libraries of heteromeric receptors
DK1471142T3 (da) * 1991-04-10 2009-03-09 Scripps Research Inst Heterodimere receptor-biblioteker under anvendelse af fagemider
US6190908B1 (en) * 1998-08-12 2001-02-20 The Scripps Research Institute Modulation of polypeptide display on modified filamentous phage

Also Published As

Publication number Publication date
CA2336541A1 (en) 2000-02-24
JP2002522084A (ja) 2002-07-23
EP1127142A1 (de) 2001-08-29
WO2000009715A1 (en) 2000-02-24
US20020146827A1 (en) 2002-10-10
US6323004B1 (en) 2001-11-27
EP1127142B1 (de) 2009-05-06
WO2000009715A9 (en) 2000-06-02
DE69940853D1 (de) 2009-06-18
US6586236B2 (en) 2003-07-01
AU5554399A (en) 2000-03-06
EP1127142A4 (de) 2004-05-26
AU766253B2 (en) 2003-10-09
CA2336541C (en) 2009-12-22
US6190908B1 (en) 2001-02-20

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