ATE415409T1 - Ein verfahren zum entschützen markierter oligonukleotide - Google Patents

Ein verfahren zum entschützen markierter oligonukleotide

Info

Publication number
ATE415409T1
ATE415409T1 AT00937721T AT00937721T ATE415409T1 AT E415409 T1 ATE415409 T1 AT E415409T1 AT 00937721 T AT00937721 T AT 00937721T AT 00937721 T AT00937721 T AT 00937721T AT E415409 T1 ATE415409 T1 AT E415409T1
Authority
AT
Austria
Prior art keywords
deprotecting
oligonucleotide
labeled oligonucleotides
deblocking
blocked
Prior art date
Application number
AT00937721T
Other languages
English (en)
Inventor
Gulilat Gebeyehu
Richard Pires
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE415409T1 publication Critical patent/ATE415409T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT00937721T 1999-05-24 2000-05-24 Ein verfahren zum entschützen markierter oligonukleotide ATE415409T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13584899P 1999-05-24 1999-05-24

Publications (1)

Publication Number Publication Date
ATE415409T1 true ATE415409T1 (de) 2008-12-15

Family

ID=22469987

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00937721T ATE415409T1 (de) 1999-05-24 2000-05-24 Ein verfahren zum entschützen markierter oligonukleotide

Country Status (8)

Country Link
US (5) US6593464B1 (de)
EP (1) EP1185544B1 (de)
JP (1) JP2003522119A (de)
AT (1) ATE415409T1 (de)
AU (1) AU5285800A (de)
CA (1) CA2375060A1 (de)
DE (1) DE60040912D1 (de)
WO (1) WO2000071559A1 (de)

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EP3034510A1 (de) * 2004-04-30 2016-06-22 Alnylam Pharmaceuticals Inc. Oligonukleotide mit c5-modifiziertem pyrimidin
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WO2006093526A2 (en) 2004-07-21 2006-09-08 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a modified or non-natural nucleobase
EP1913011B1 (de) 2004-08-04 2016-11-02 Alnylam Pharmaceuticals Inc. Oligonukleotide mit einem an einer modifizierten oder nicht-natürlichen nukleobase angebundenen liganden
DK3030682T3 (da) 2013-08-05 2020-09-14 Twist Bioscience Corp De novo synthesized gene libraries
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
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IL258164B (en) 2015-09-18 2022-09-01 Twist Bioscience Corp Methods to regulate the activity of proteins and cells and a method for the production of nucleic acids
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
EP3384077A4 (de) 2015-12-01 2019-05-08 Twist Bioscience Corporation Funktionalisierte oberflächen und herstellung davon
JP6854340B2 (ja) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション デノボ合成された核酸ライブラリ
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US10907274B2 (en) 2016-12-16 2021-02-02 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN118116478A (zh) 2017-02-22 2024-05-31 特韦斯特生物科学公司 基于核酸的数据存储
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
CA3079613A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
WO2019136175A1 (en) 2018-01-04 2019-07-11 Twist Bioscience Corporation Dna-based digital information storage
AU2019270243A1 (en) 2018-05-18 2021-01-07 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CN113766930A (zh) 2019-02-26 2021-12-07 特韦斯特生物科学公司 Glp1受体的变异核酸文库
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2

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Also Published As

Publication number Publication date
US8524882B2 (en) 2013-09-03
US20120253028A1 (en) 2012-10-04
EP1185544A1 (de) 2002-03-13
US9085797B2 (en) 2015-07-21
EP1185544B1 (de) 2008-11-26
DE60040912D1 (de) 2009-01-08
WO2000071559A1 (en) 2000-11-30
US20160024138A1 (en) 2016-01-28
JP2003522119A (ja) 2003-07-22
US8093372B2 (en) 2012-01-10
AU5285800A (en) 2000-12-12
US6593464B1 (en) 2003-07-15
US20030120063A1 (en) 2003-06-26
CA2375060A1 (en) 2000-11-30
WO2000071559A9 (en) 2001-06-21
EP1185544A4 (de) 2002-07-17
US20140066612A1 (en) 2014-03-06

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