ATE415409T1 - Ein verfahren zum entschützen markierter oligonukleotide - Google Patents

Ein verfahren zum entschützen markierter oligonukleotide

Info

Publication number
ATE415409T1
ATE415409T1 AT00937721T AT00937721T ATE415409T1 AT E415409 T1 ATE415409 T1 AT E415409T1 AT 00937721 T AT00937721 T AT 00937721T AT 00937721 T AT00937721 T AT 00937721T AT E415409 T1 ATE415409 T1 AT E415409T1
Authority
AT
Austria
Prior art keywords
deprotecting
oligonucleotide
labeled oligonucleotides
deblocking
blocked
Prior art date
Application number
AT00937721T
Other languages
English (en)
Inventor
Gulilat Gebeyehu
Richard Pires
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE415409T1 publication Critical patent/ATE415409T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07BGENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11Compounds covalently bound to a solid support

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT00937721T 1999-05-24 2000-05-24 Ein verfahren zum entschützen markierter oligonukleotide ATE415409T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13584899P 1999-05-24 1999-05-24

Publications (1)

Publication Number Publication Date
ATE415409T1 true ATE415409T1 (de) 2008-12-15

Family

ID=22469987

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00937721T ATE415409T1 (de) 1999-05-24 2000-05-24 Ein verfahren zum entschützen markierter oligonukleotide

Country Status (8)

Country Link
US (5) US6593464B1 (de)
EP (1) EP1185544B1 (de)
JP (1) JP2003522119A (de)
AT (1) ATE415409T1 (de)
AU (1) AU5285800A (de)
CA (1) CA2375060A1 (de)
DE (1) DE60040912D1 (de)
WO (1) WO2000071559A1 (de)

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HK1258869A1 (zh) 2015-09-18 2019-11-22 Twist Bioscience Corporation 寡核酸变体文库及其合成
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
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US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
EP3595674A4 (de) 2017-03-15 2020-12-16 Twist Bioscience Corporation Variante bibliotheken der immunologischen synapse und synthese davon
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
GB2581620A (en) 2017-09-11 2020-08-26 Twist Bioscience Corp GPCR binding proteins and synthesis thereof
CA3079613A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
GB2585506A (en) 2018-01-04 2021-01-13 Twist Bioscience Corp DNA-based digital information storage
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
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AU2020227802A1 (en) 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
JP2022521551A (ja) 2019-02-26 2022-04-08 ツイスト バイオサイエンス コーポレーション Glp1受容体の変異体核酸ライブラリ
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
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Also Published As

Publication number Publication date
US20030120063A1 (en) 2003-06-26
EP1185544A4 (de) 2002-07-17
US9085797B2 (en) 2015-07-21
EP1185544B1 (de) 2008-11-26
EP1185544A1 (de) 2002-03-13
US20140066612A1 (en) 2014-03-06
AU5285800A (en) 2000-12-12
US8524882B2 (en) 2013-09-03
JP2003522119A (ja) 2003-07-22
DE60040912D1 (de) 2009-01-08
WO2000071559A1 (en) 2000-11-30
US6593464B1 (en) 2003-07-15
WO2000071559A9 (en) 2001-06-21
US8093372B2 (en) 2012-01-10
US20160024138A1 (en) 2016-01-28
CA2375060A1 (en) 2000-11-30
US20120253028A1 (en) 2012-10-04

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