ATE415409T1 - Ein verfahren zum entschützen markierter oligonukleotide - Google Patents
Ein verfahren zum entschützen markierter oligonukleotideInfo
- Publication number
- ATE415409T1 ATE415409T1 AT00937721T AT00937721T ATE415409T1 AT E415409 T1 ATE415409 T1 AT E415409T1 AT 00937721 T AT00937721 T AT 00937721T AT 00937721 T AT00937721 T AT 00937721T AT E415409 T1 ATE415409 T1 AT E415409T1
- Authority
- AT
- Austria
- Prior art keywords
- deprotecting
- oligonucleotide
- labeled oligonucleotides
- deblocking
- blocked
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 5
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- -1 amino compound Chemical class 0.000 abstract 1
- 230000000269 nucleophilic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/11—Compounds covalently bound to a solid support
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13584899P | 1999-05-24 | 1999-05-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE415409T1 true ATE415409T1 (de) | 2008-12-15 |
Family
ID=22469987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00937721T ATE415409T1 (de) | 1999-05-24 | 2000-05-24 | Ein verfahren zum entschützen markierter oligonukleotide |
Country Status (8)
| Country | Link |
|---|---|
| US (5) | US6593464B1 (de) |
| EP (1) | EP1185544B1 (de) |
| JP (1) | JP2003522119A (de) |
| AT (1) | ATE415409T1 (de) |
| AU (1) | AU5285800A (de) |
| CA (1) | CA2375060A1 (de) |
| DE (1) | DE60040912D1 (de) |
| WO (1) | WO2000071559A1 (de) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003500070A (ja) * | 1999-05-21 | 2003-01-07 | インビトロゲン・コーポレーション | 核酸分子を標識するための組成物および方法 |
| DE60040912D1 (de) * | 1999-05-24 | 2009-01-08 | Invitrogen Corp | Ein verfahren zum entschützen markierter oligonukleotide |
| CA2561741C (en) | 2004-04-05 | 2016-09-27 | Alnylam Pharmaceuticals, Inc. | Processes and reagents for oligonucleotide synthesis and purification |
| US7626014B2 (en) | 2004-04-27 | 2009-12-01 | Alnylam Pharmaceuticals | Single-stranded and double-stranded oligonucleotides comprising a 2-arylpropyl moiety |
| AU2005323437B2 (en) * | 2004-04-30 | 2011-10-06 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a C5-modified pyrimidine |
| CA2572151A1 (en) * | 2004-06-30 | 2006-08-24 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a non-phosphate backbone linkage |
| WO2006093526A2 (en) | 2004-07-21 | 2006-09-08 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a modified or non-natural nucleobase |
| US7632932B2 (en) | 2004-08-04 | 2009-12-15 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase |
| TWI707038B (zh) | 2013-08-05 | 2020-10-11 | 美商扭轉生物科技有限公司 | 重新合成之基因庫 |
| WO2016126882A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| CA2975855C (en) | 2015-02-04 | 2025-09-23 | Twist Bioscience Corporation | SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS |
| US9981239B2 (en) | 2015-04-21 | 2018-05-29 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| HK1258869A1 (zh) | 2015-09-18 | 2019-11-22 | Twist Bioscience Corporation | 寡核酸变体文库及其合成 |
| CN108698012A (zh) | 2015-09-22 | 2018-10-23 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
| US9895673B2 (en) | 2015-12-01 | 2018-02-20 | Twist Bioscience Corporation | Functionalized surfaces and preparation thereof |
| SG11201901563UA (en) | 2016-08-22 | 2019-03-28 | Twist Bioscience Corp | De novo synthesized nucleic acid libraries |
| US10417457B2 (en) | 2016-09-21 | 2019-09-17 | Twist Bioscience Corporation | Nucleic acid based data storage |
| AU2017378492B2 (en) | 2016-12-16 | 2022-06-16 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| US11550939B2 (en) | 2017-02-22 | 2023-01-10 | Twist Bioscience Corporation | Nucleic acid based data storage using enzymatic bioencryption |
| EP3595674A4 (de) | 2017-03-15 | 2020-12-16 | Twist Bioscience Corporation | Variante bibliotheken der immunologischen synapse und synthese davon |
| IL271205B2 (en) | 2017-06-12 | 2025-02-01 | Twist Bioscience Corp | Methods for assembling continuous nucleic acids |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| GB2581620A (en) | 2017-09-11 | 2020-08-26 | Twist Bioscience Corp | GPCR binding proteins and synthesis thereof |
| CA3079613A1 (en) | 2017-10-20 | 2019-04-25 | Twist Bioscience Corporation | Heated nanowells for polynucleotide synthesis |
| GB2585506A (en) | 2018-01-04 | 2021-01-13 | Twist Bioscience Corp | DNA-based digital information storage |
| CA3100739A1 (en) | 2018-05-18 | 2019-11-21 | Twist Bioscience Corporation | Polynucleotides, reagents, and methods for nucleic acid hybridization |
| EP3902816A4 (de) | 2018-12-26 | 2022-09-14 | Twist Bioscience Corporation | Hochgenaue de-novo-polynukleotidsynthese |
| AU2020227802A1 (en) | 2019-02-26 | 2021-10-14 | Twist Bioscience Corporation | Variant nucleic acid libraries for antibody optimization |
| JP2022521551A (ja) | 2019-02-26 | 2022-04-08 | ツイスト バイオサイエンス コーポレーション | Glp1受容体の変異体核酸ライブラリ |
| AU2020298294A1 (en) | 2019-06-21 | 2022-02-17 | Twist Bioscience Corporation | Barcode-based nucleic acid sequence assembly |
| US12091777B2 (en) | 2019-09-23 | 2024-09-17 | Twist Bioscience Corporation | Variant nucleic acid libraries for CRTH2 |
| EP4034564A4 (de) | 2019-09-23 | 2023-12-13 | Twist Bioscience Corporation | Variante nukleinsäurebibliotheken für einzeldomänenantikörper |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4055469A (en) | 1976-12-10 | 1977-10-25 | Eastman Kodak Company | Purification of microbial enzyme extracts using synthetic polyelectrolytes |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
| CA1219824A (en) | 1981-04-17 | 1987-03-31 | David C. Ward | Modified nucleotides and methods of preparing and using same |
| US4419509A (en) | 1981-08-24 | 1983-12-06 | Eli Lilly And Company | Process for de-cyanoethylating blocked nucleotides |
| ZA831626B (en) | 1982-08-25 | 1983-11-30 | Velsicol Chemical Corp | New triazine compositions of matter |
| US5539097A (en) * | 1983-09-02 | 1996-07-23 | Molecular Biosystems, Inc. | Oligonucleotide polymeric support system |
| IT1202321B (it) | 1985-06-06 | 1989-02-02 | Uni Degli Studi Di Parmo | Nucleotidi ciclici fluorescenti biologicamente attivi |
| US4739044A (en) | 1985-06-13 | 1988-04-19 | Amgen | Method for derivitization of polynucleotides |
| US4757141A (en) | 1985-08-26 | 1988-07-12 | Applied Biosystems, Incorporated | Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof |
| US4965349A (en) * | 1987-12-24 | 1990-10-23 | Applied Biosystems, Inc. | Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports |
| US4997928A (en) | 1988-09-15 | 1991-03-05 | E. I. Du Pont De Nemours And Company | Fluorescent reagents for the preparation of 5'-tagged oligonucleotides |
| US5262536A (en) | 1988-09-15 | 1993-11-16 | E. I. Du Pont De Nemours And Company | Reagents for the preparation of 5'-tagged oligonucleotides |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5428148A (en) | 1992-04-24 | 1995-06-27 | Beckman Instruments, Inc. | N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis |
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| US5472672A (en) | 1993-10-22 | 1995-12-05 | The Board Of Trustees Of The Leland Stanford Junior University | Apparatus and method for polymer synthesis using arrays |
| PT728218E (pt) | 1993-11-12 | 2008-04-08 | Phri Properties Inc | Sondas de hibridação para detecção de ácidos nucleicos, troncos universais, métodos e estojos |
| US5736480A (en) | 1994-02-22 | 1998-04-07 | California Institute Of Technology | Supported phase chiral sulfonated BINAP catalyst solubilized in alcohol and method of asymmetric hydrogenation |
| US5623068A (en) * | 1994-03-07 | 1997-04-22 | Beckman Instruments, Inc. | Synthesis of DNA using substituted phenylacetyl-protected nucleotides |
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| US5514789A (en) | 1994-04-21 | 1996-05-07 | Barrskogen, Inc. | Recovery of oligonucleotides by gas phase cleavage |
| US5888397A (en) | 1995-06-07 | 1999-03-30 | Arch Develop. Corp. | Process for recovering chaotropic anions from an aqueous solution also containing other ions |
| WO1997026245A1 (en) * | 1996-01-16 | 1997-07-24 | Lumigen, Inc. | Compounds, compositions and methods for generating chemiluminescence with phosphatase enzymes |
| US5736626A (en) * | 1996-01-29 | 1998-04-07 | The Perkin-Elmer Corporation | Solid support reagents for the direct synthesis of 3'-labeled polynucleotides |
| US5942608A (en) | 1996-10-29 | 1999-08-24 | Council of Sciemtific & Industrial Research and Department of Biotechnology | Process for preparing a universal support for the synthesis of oligonucleotides |
| ATE414167T1 (de) * | 1996-11-29 | 2008-11-15 | Third Wave Tech Inc | Fen-1 endonukleasen, gemische und spaltmethoden |
| US5756705A (en) * | 1997-03-05 | 1998-05-26 | Wang; Edge Renfeng | Method for labeling oligonucleotide with ammonia-sensitive ligands |
| US5773590A (en) | 1997-04-08 | 1998-06-30 | Betzdearborn Inc. | Methods of separating lignin solids and black liquor from tall oil fatty acids |
| US6583168B1 (en) * | 1997-11-25 | 2003-06-24 | Applera Corporation | Sulfonated diarylrhodamine dyes |
| WO1999038962A2 (en) | 1998-02-02 | 1999-08-05 | Gentra Systems, Inc. | Compositions and methods for using a lysing matrix for isolating dna |
| US6468743B1 (en) * | 1998-05-18 | 2002-10-22 | Conagra Grocery Products Company | PCR techniques for detecting microbial contaminants in foodstuffs |
| US5942609A (en) * | 1998-11-12 | 1999-08-24 | The Porkin-Elmer Corporation | Ligation assembly and detection of polynucleotides on solid-support |
| US6255476B1 (en) * | 1999-02-22 | 2001-07-03 | Pe Corporation (Ny) | Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports |
| DE60040912D1 (de) * | 1999-05-24 | 2009-01-08 | Invitrogen Corp | Ein verfahren zum entschützen markierter oligonukleotide |
| JP2004513629A (ja) | 2000-11-09 | 2004-05-13 | インヴィトロジェン コーポレーション | オリゴヌクレオチドからユニバーサルリンカーを除去する方法 |
| AU2002226053A1 (en) | 2000-12-12 | 2002-06-24 | Invitrogen Corporation | Compositions and methods for the release of nucleic acid molecules from solid matrices |
| JP2004521628A (ja) * | 2001-03-08 | 2004-07-22 | アプレラ コーポレイション | オリゴヌクレオチドの切断および脱保護のための試薬 |
-
2000
- 2000-05-24 DE DE60040912T patent/DE60040912D1/de not_active Expired - Fee Related
- 2000-05-24 EP EP00937721A patent/EP1185544B1/de not_active Expired - Lifetime
- 2000-05-24 AU AU52858/00A patent/AU5285800A/en not_active Abandoned
- 2000-05-24 CA CA002375060A patent/CA2375060A1/en not_active Abandoned
- 2000-05-24 JP JP2000619814A patent/JP2003522119A/ja not_active Ceased
- 2000-05-24 WO PCT/US2000/014274 patent/WO2000071559A1/en not_active Ceased
- 2000-05-24 AT AT00937721T patent/ATE415409T1/de not_active IP Right Cessation
- 2000-05-24 US US09/576,555 patent/US6593464B1/en not_active Expired - Lifetime
-
2003
- 2003-02-13 US US10/365,644 patent/US8093372B2/en not_active Expired - Fee Related
-
2011
- 2011-06-03 US US13/152,907 patent/US8524882B2/en not_active Expired - Fee Related
-
2013
- 2013-08-09 US US13/964,036 patent/US9085797B2/en not_active Expired - Fee Related
-
2015
- 2015-06-24 US US14/748,930 patent/US20160024138A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20030120063A1 (en) | 2003-06-26 |
| EP1185544A4 (de) | 2002-07-17 |
| US9085797B2 (en) | 2015-07-21 |
| EP1185544B1 (de) | 2008-11-26 |
| EP1185544A1 (de) | 2002-03-13 |
| US20140066612A1 (en) | 2014-03-06 |
| AU5285800A (en) | 2000-12-12 |
| US8524882B2 (en) | 2013-09-03 |
| JP2003522119A (ja) | 2003-07-22 |
| DE60040912D1 (de) | 2009-01-08 |
| WO2000071559A1 (en) | 2000-11-30 |
| US6593464B1 (en) | 2003-07-15 |
| WO2000071559A9 (en) | 2001-06-21 |
| US8093372B2 (en) | 2012-01-10 |
| US20160024138A1 (en) | 2016-01-28 |
| CA2375060A1 (en) | 2000-11-30 |
| US20120253028A1 (en) | 2012-10-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |