ATE412776T1 - Verfahren zur suche eines genes, welches ein protein des nukleären transportes kodiert - Google Patents
Verfahren zur suche eines genes, welches ein protein des nukleären transportes kodiertInfo
- Publication number
- ATE412776T1 ATE412776T1 AT01979034T AT01979034T ATE412776T1 AT E412776 T1 ATE412776 T1 AT E412776T1 AT 01979034 T AT01979034 T AT 01979034T AT 01979034 T AT01979034 T AT 01979034T AT E412776 T1 ATE412776 T1 AT E412776T1
- Authority
- AT
- Austria
- Prior art keywords
- cells
- gene
- nuclei
- searching
- codes
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 7
- 102000014914 Carrier Proteins Human genes 0.000 title 1
- 108010078791 Carrier Proteins Proteins 0.000 title 1
- 230000025308 nuclear transport Effects 0.000 title 1
- 102000037865 fusion proteins Human genes 0.000 abstract 3
- 108020001507 fusion proteins Proteins 0.000 abstract 3
- 230000014509 gene expression Effects 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000337906 | 2000-11-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE412776T1 true ATE412776T1 (de) | 2008-11-15 |
Family
ID=18813213
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01979034T ATE412776T1 (de) | 2000-11-06 | 2001-11-06 | Verfahren zur suche eines genes, welches ein protein des nukleären transportes kodiert |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7195869B2 (de) |
| EP (1) | EP1333099B1 (de) |
| JP (1) | JP3678233B2 (de) |
| AT (1) | ATE412776T1 (de) |
| AU (1) | AU2002211021A1 (de) |
| DE (1) | DE60136380D1 (de) |
| WO (1) | WO2002036823A1 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0203924D0 (en) * | 2002-02-19 | 2002-04-03 | Univ Bristol | Screening method |
| WO2006046504A1 (ja) * | 2004-10-25 | 2006-05-04 | Tohoku University | シグナル依存性核内移行蛋白質遺伝子の取得方法 |
| GB0526449D0 (en) * | 2005-12-23 | 2006-02-08 | Medical Res Council | Polypeptide targeting |
| US20100285446A1 (en) * | 2007-07-20 | 2010-11-11 | Akos Vertes | Methods for Detecting Metabolic States by Laser Ablation Electrospray Ionization Mass Spectrometry |
| US8067730B2 (en) | 2007-07-20 | 2011-11-29 | The George Washington University | Laser ablation electrospray ionization (LAESI) for atmospheric pressure, In vivo, and imaging mass spectrometry |
| US8901487B2 (en) * | 2007-07-20 | 2014-12-02 | George Washington University | Subcellular analysis by laser ablation electrospray ionization mass spectrometry |
| EP2732457A4 (de) | 2011-07-14 | 2015-09-16 | Univ George Washington | Abgaskollimation für eine massenspektrometrie mit laserablations-elektrospray-ionisation |
| CN102533738B (zh) | 2012-03-15 | 2013-07-31 | 田敬东 | 一种基因合成方法、基因芯片及试剂盒 |
| CN118256535B (zh) * | 2024-03-06 | 2025-01-14 | 沃臻生物科技(佛山)有限公司 | 一种表达Mouse SPO11蛋白特定结构域的方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5891924A (en) | 1996-09-26 | 1999-04-06 | Research Development Foundation | Curcumin (diferuloylmethane) inhibition of NFκB activation |
| AU6048599A (en) | 1998-09-18 | 2000-04-10 | Cellomics, Inc. | A system for cell-based screening |
| WO2000023615A2 (en) * | 1998-10-15 | 2000-04-27 | Bioimage A/S | Method for extracting quantitative information relating to an influence on a cellular response |
| AU3431700A (en) * | 1999-03-23 | 2000-10-09 | Europaisches Laboratorium Fur Molekularbiologie (Embl) | Detection, cloning and sequencing of polypeptides which drive the subcellular localization of proteins |
| FR2811681A1 (fr) * | 2000-07-17 | 2002-01-18 | Commissariat Energie Atomique | Procede de criblage de la translocation nucleo-cytoplasmique de proteines sous l'effet d'un stimulus, d'elements y etant impliques et kits de mise en oeuvre |
-
2001
- 2001-11-06 EP EP01979034A patent/EP1333099B1/de not_active Expired - Lifetime
- 2001-11-06 US US10/204,310 patent/US7195869B2/en not_active Expired - Fee Related
- 2001-11-06 WO PCT/JP2001/009700 patent/WO2002036823A1/ja not_active Ceased
- 2001-11-06 AU AU2002211021A patent/AU2002211021A1/en not_active Abandoned
- 2001-11-06 JP JP2002539566A patent/JP3678233B2/ja not_active Expired - Fee Related
- 2001-11-06 DE DE60136380T patent/DE60136380D1/de not_active Expired - Lifetime
- 2001-11-06 AT AT01979034T patent/ATE412776T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002211021A1 (en) | 2002-05-15 |
| WO2002036823A1 (fr) | 2002-05-10 |
| JPWO2002036823A1 (ja) | 2004-03-11 |
| JP3678233B2 (ja) | 2005-08-03 |
| DE60136380D1 (de) | 2008-12-11 |
| US20030180801A1 (en) | 2003-09-25 |
| EP1333099A4 (de) | 2005-11-16 |
| EP1333099A1 (de) | 2003-08-06 |
| US7195869B2 (en) | 2007-03-27 |
| EP1333099B1 (de) | 2008-10-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |