AU2002211021A1 - Method of searching for gene encoding nuclear transport protein - Google Patents

Method of searching for gene encoding nuclear transport protein

Info

Publication number
AU2002211021A1
AU2002211021A1 AU2002211021A AU1102102A AU2002211021A1 AU 2002211021 A1 AU2002211021 A1 AU 2002211021A1 AU 2002211021 A AU2002211021 A AU 2002211021A AU 1102102 A AU1102102 A AU 1102102A AU 2002211021 A1 AU2002211021 A1 AU 2002211021A1
Authority
AU
Australia
Prior art keywords
cells
nuclei
searching
gene encoding
transport protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002211021A
Inventor
Takami Maekawa
Maiko Mori
Yoshiyuki Takahara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Publication of AU2002211021A1 publication Critical patent/AU2002211021A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Abstract

Each gene in a group comprising many genes is cloned into an expression vector so that its gene product should be expressed as a fusion protein with a protein emitting self-fluorescence to construct a gene expression library. The gene expression library is introduced into cells of two groups to express the fusion proteins in the cells, and only one cell group is stimulated by a drug or the like. Then, cells in which the fusion proteins are localized in the nuclei or their nuclei are isolated from cells of the two groups, respectively. By comparing these cells, genes coding for proteins that are transported into the nuclei specifically in response to a certain stimulus when the stimulus is applied to the cells are retrieved. <IMAGE>
AU2002211021A 2000-11-06 2001-11-06 Method of searching for gene encoding nuclear transport protein Abandoned AU2002211021A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2000337906 2000-11-06
JP2000-337906 2000-11-06
PCT/JP2001/009700 WO2002036823A1 (en) 2000-11-06 2001-11-06 Method of searching for gene encoding nuclear transport protein

Publications (1)

Publication Number Publication Date
AU2002211021A1 true AU2002211021A1 (en) 2002-05-15

Family

ID=18813213

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002211021A Abandoned AU2002211021A1 (en) 2000-11-06 2001-11-06 Method of searching for gene encoding nuclear transport protein

Country Status (7)

Country Link
US (1) US7195869B2 (en)
EP (1) EP1333099B1 (en)
JP (1) JP3678233B2 (en)
AT (1) ATE412776T1 (en)
AU (1) AU2002211021A1 (en)
DE (1) DE60136380D1 (en)
WO (1) WO2002036823A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0203924D0 (en) * 2002-02-19 2002-04-03 Univ Bristol Screening method
WO2006046504A1 (en) * 2004-10-25 2006-05-04 Tohoku University Method of acquiring signal-dependent nuclear transport protein gene
GB0526449D0 (en) * 2005-12-23 2006-02-08 Medical Res Council Polypeptide targeting
US20100285446A1 (en) * 2007-07-20 2010-11-11 Akos Vertes Methods for Detecting Metabolic States by Laser Ablation Electrospray Ionization Mass Spectrometry
US8067730B2 (en) 2007-07-20 2011-11-29 The George Washington University Laser ablation electrospray ionization (LAESI) for atmospheric pressure, In vivo, and imaging mass spectrometry
US8901487B2 (en) * 2007-07-20 2014-12-02 George Washington University Subcellular analysis by laser ablation electrospray ionization mass spectrometry
WO2013085572A2 (en) 2011-07-14 2013-06-13 The George Washington University Plume collimation for laser ablation electrospray ionization mass spectrometry
CN102533738B (en) 2012-03-15 2013-07-31 田敬东 Gene synthesizing method, gene chip and kit

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891924A (en) * 1996-09-26 1999-04-06 Research Development Foundation Curcumin (diferuloylmethane) inhibition of NFκB activation
WO2000017643A2 (en) * 1998-09-18 2000-03-30 Cellomics, Inc. A system for cell-based screening
EP1121593A2 (en) 1998-10-15 2001-08-08 BioImage A/S Method for extracting quantitative information relating to an influence on a cellular response
AU3431700A (en) * 1999-03-23 2000-10-09 Europaisches Laboratorium Fur Molekularbiologie (Embl) Detection, cloning and sequencing of polypeptides which drive the subcellular localization of proteins
FR2811681A1 (en) * 2000-07-17 2002-01-18 Commissariat Energie Atomique Vector for identifying modulators of nuclear-cytoplasmic protein translocation, useful e.g. for targeted delivery of therapeutic proteins

Also Published As

Publication number Publication date
EP1333099A4 (en) 2005-11-16
US20030180801A1 (en) 2003-09-25
WO2002036823A1 (en) 2002-05-10
EP1333099A1 (en) 2003-08-06
US7195869B2 (en) 2007-03-27
JPWO2002036823A1 (en) 2004-03-11
JP3678233B2 (en) 2005-08-03
ATE412776T1 (en) 2008-11-15
DE60136380D1 (en) 2008-12-11
EP1333099B1 (en) 2008-10-29

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