ATE404665T1 - Methode zur herstellung hypermutierender bakterien - Google Patents
Methode zur herstellung hypermutierender bakterienInfo
- Publication number
- ATE404665T1 ATE404665T1 AT01907187T AT01907187T ATE404665T1 AT E404665 T1 ATE404665 T1 AT E404665T1 AT 01907187 T AT01907187 T AT 01907187T AT 01907187 T AT01907187 T AT 01907187T AT E404665 T1 ATE404665 T1 AT E404665T1
- Authority
- AT
- Austria
- Prior art keywords
- bacteria
- mismatch repair
- hypermutating
- producing
- desirable output
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title 1
- 230000033607 mismatch repair Effects 0.000 abstract 3
- 108700028369 Alleles Proteins 0.000 abstract 1
- 230000007812 deficiency Effects 0.000 abstract 1
- 239000003471 mutagenic agent Substances 0.000 abstract 1
- 231100000707 mutagenic chemical Toxicity 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18192900P | 2000-02-11 | 2000-02-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE404665T1 true ATE404665T1 (de) | 2008-08-15 |
Family
ID=22666395
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01907187T ATE404665T1 (de) | 2000-02-11 | 2001-02-12 | Methode zur herstellung hypermutierender bakterien |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US7026119B2 (de) |
| EP (1) | EP1268765B1 (de) |
| AT (1) | ATE404665T1 (de) |
| AU (1) | AU2001234992A1 (de) |
| CA (1) | CA2398094C (de) |
| DE (1) | DE60135312D1 (de) |
| WO (1) | WO2001059092A2 (de) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6365410B1 (en) * | 1999-05-19 | 2002-04-02 | Genencor International, Inc. | Directed evolution of microorganisms |
| DE60135312D1 (de) * | 2000-02-11 | 2008-09-25 | Morphotek Inc | Methode zur herstellung hypermutierender bakterien |
| US6825038B2 (en) | 2000-05-12 | 2004-11-30 | The Johns Hopkins University | Method for generating hypermutable organisms |
| US6737268B1 (en) * | 2000-11-14 | 2004-05-18 | Morphotek, Inc. | Method for generating genetically altered antigens |
| ATE548461T1 (de) | 2002-01-18 | 2012-03-15 | Morphotek Inc | Verfahren zur erzeugung manipulierter zellen zur locus-spezifischen genregulation und analyse |
| JP2005531291A (ja) * | 2002-02-21 | 2005-10-20 | モーフオテク・インコーポレーテツド | Ｐｍｓｒ相同体を使用する超変異性細胞の作成方法 |
| US20030165468A1 (en) * | 2002-02-21 | 2003-09-04 | Luigi Grasso | Regulated vectors for controlling DNA hypermutability in eukaryotic cells |
| CA2506127C (en) | 2002-11-15 | 2013-07-09 | Morphotek, Inc. | Methods of generating high-production of antibodies from hybridomas created by in vitro immunization |
| WO2006032504A2 (en) * | 2004-09-22 | 2006-03-30 | Vrije Universiteit Brussel | Method for introducing genetic mutations into plant cells |
| US20090068741A1 (en) * | 2007-01-17 | 2009-03-12 | Invitrogen Corporation | Methods and Compositions for Improving the Health of Cells in Culture |
| ES2628973T3 (es) | 2007-05-31 | 2017-08-04 | University Of Washington | Mutagénesis inducible de genes diana |
| CN102911962B (zh) * | 2011-08-02 | 2017-03-22 | 中国科学院微生物研究所 | 改造微生物获得改进表型的方法 |
| WO2013138518A1 (en) | 2012-03-15 | 2013-09-19 | Omeros Corporation | Composition and method for diversification of target sequences |
| US20190119667A1 (en) * | 2017-09-16 | 2019-04-25 | Massachusetts Institute Of Technology | Methods for Directed Exaptation |
| CN108753814B (zh) * | 2018-06-11 | 2022-10-18 | 北京师范大学 | 一种加速物种突变的新育种方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6191268B1 (en) | 1993-12-17 | 2001-02-20 | Dana-Farber Cancer Institute | Compositions and methods relating to DNA mismatch repair genes |
| US5843757A (en) | 1995-08-24 | 1998-12-01 | The Johns Hopkins University | Human JTV1 gene overlaps PMS2 gene |
| US5907079A (en) | 1996-01-18 | 1999-05-25 | Amgen Canada Inc. | MSH2 disrupted mice develop lymphomas |
| US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| AUPO974597A0 (en) | 1997-10-10 | 1997-11-06 | Rhone-Poulenc Agro | Methods for obtaining plant varieties |
| US6146894A (en) | 1998-04-14 | 2000-11-14 | The Johns Hopkins University | Method for generating hypermutable organisms |
| DE60135312D1 (de) * | 2000-02-11 | 2008-09-25 | Morphotek Inc | Methode zur herstellung hypermutierender bakterien |
| ATE351914T1 (de) * | 2000-02-23 | 2007-02-15 | Univ Johns Hopkins | Verfahren zur erzeugung von schnell mutierender hefe |
| US6737268B1 (en) * | 2000-11-14 | 2004-05-18 | Morphotek, Inc. | Method for generating genetically altered antigens |
-
2001
- 2001-02-12 DE DE60135312T patent/DE60135312D1/de not_active Expired - Fee Related
- 2001-02-12 AU AU2001234992A patent/AU2001234992A1/en not_active Abandoned
- 2001-02-12 WO PCT/US2001/004339 patent/WO2001059092A2/en not_active Ceased
- 2001-02-12 US US09/780,675 patent/US7026119B2/en not_active Expired - Lifetime
- 2001-02-12 EP EP01907187A patent/EP1268765B1/de not_active Expired - Lifetime
- 2001-02-12 AT AT01907187T patent/ATE404665T1/de not_active IP Right Cessation
- 2001-02-12 CA CA2398094A patent/CA2398094C/en not_active Expired - Lifetime
-
2006
- 2006-02-24 US US11/360,995 patent/US7695969B2/en not_active Expired - Lifetime
-
2007
- 2007-10-31 US US11/930,391 patent/US7892832B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1268765B1 (de) | 2008-08-13 |
| US7026119B2 (en) | 2006-04-11 |
| CA2398094C (en) | 2013-01-08 |
| US20060194236A1 (en) | 2006-08-31 |
| WO2001059092A9 (en) | 2002-10-24 |
| US7695969B2 (en) | 2010-04-13 |
| CA2398094A1 (en) | 2001-08-16 |
| WO2001059092A3 (en) | 2002-07-25 |
| EP1268765A2 (de) | 2003-01-02 |
| US7892832B2 (en) | 2011-02-22 |
| AU2001234992A1 (en) | 2001-08-20 |
| US20080318321A1 (en) | 2008-12-25 |
| WO2001059092A2 (en) | 2001-08-16 |
| DE60135312D1 (de) | 2008-09-25 |
| US20020068284A1 (en) | 2002-06-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |