ATE548461T1 - Verfahren zur erzeugung manipulierter zellen zur locus-spezifischen genregulation und analyse - Google Patents
Verfahren zur erzeugung manipulierter zellen zur locus-spezifischen genregulation und analyseInfo
- Publication number
- ATE548461T1 ATE548461T1 AT03703842T AT03703842T ATE548461T1 AT E548461 T1 ATE548461 T1 AT E548461T1 AT 03703842 T AT03703842 T AT 03703842T AT 03703842 T AT03703842 T AT 03703842T AT E548461 T1 ATE548461 T1 AT E548461T1
- Authority
- AT
- Austria
- Prior art keywords
- locus
- analysis
- cells
- specific gene
- gene regulation
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 108700024394 Exon Proteins 0.000 abstract 1
- 150000005829 chemical entities Chemical class 0.000 abstract 1
- 230000006801 homologous recombination Effects 0.000 abstract 1
- 238000002744 homologous recombination Methods 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 230000033607 mismatch repair Effects 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34956502P | 2002-01-18 | 2002-01-18 | |
| PCT/US2003/001361 WO2003062435A1 (en) | 2002-01-18 | 2003-01-17 | A method for generating engineered cells for locus specific gene regulation and analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE548461T1 true ATE548461T1 (de) | 2012-03-15 |
Family
ID=27613291
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03703842T ATE548461T1 (de) | 2002-01-18 | 2003-01-17 | Verfahren zur erzeugung manipulierter zellen zur locus-spezifischen genregulation und analyse |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7638334B2 (de) |
| EP (1) | EP1474522B1 (de) |
| AT (1) | ATE548461T1 (de) |
| AU (1) | AU2003205173C1 (de) |
| CA (1) | CA2473741C (de) |
| WO (1) | WO2003062435A1 (de) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6982169B2 (en) * | 2001-01-15 | 2006-01-03 | Morphotek, Inc. | Chemical inhibitors of mismatch repair |
| US7754450B2 (en) | 2002-11-15 | 2010-07-13 | Morphotek, Inc. | Methods of generating high-production of antibodies from hybridomas created by in vitro immunization |
| WO2011058555A1 (en) * | 2009-11-12 | 2011-05-19 | Yeda Research And Development Co. Ltd. | A method of editing dna in a cell and constructs capable of same |
| EP2814969A4 (de) * | 2012-02-10 | 2016-02-17 | Univ Minnesota | Dna-assimilation |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2641793B1 (fr) * | 1988-12-26 | 1993-10-01 | Setratech | Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases |
| US5272071A (en) * | 1989-12-22 | 1993-12-21 | Applied Research Systems Ars Holding N.V. | Method for the modification of the expression characteristics of an endogenous gene of a given cell line |
| US5922601A (en) * | 1995-01-19 | 1999-07-13 | Biotransplant, Inc. | High efficiency gene trap selection of regulated genetic loci |
| US5681744A (en) * | 1995-03-17 | 1997-10-28 | Greenstein; Robert J. | Delivery and expression of heterologus genes using upstream enhancer regions of mammalian gene promoters |
| WO1997005268A1 (en) | 1995-07-26 | 1997-02-13 | Setratech | Homologous recombination in mismatch repair inactivated eukaryotic cells |
| CN1254376A (zh) * | 1996-10-01 | 2000-05-24 | 杰龙公司 | 端粒酶逆转录酶 |
| DE69841340D1 (de) * | 1997-12-05 | 2010-01-07 | Europ Lab Molekularbiolog | Neue Methode zur Klonierung DNS unter Anwendung des E. coli RecE/RecT Rekombinationssystems |
| US6146894A (en) * | 1998-04-14 | 2000-11-14 | The Johns Hopkins University | Method for generating hypermutable organisms |
| US6355412B1 (en) * | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
| EP1268765B1 (de) | 2000-02-11 | 2008-08-13 | The Johns Hopkins University | Methode zur herstellung hypermutierender bakterien |
| EP1282708B1 (de) | 2000-02-18 | 2008-07-02 | Morphotek, Inc. | Methode zur herstellung hypermutabler pflanzen |
| DK1259628T5 (da) * | 2000-02-23 | 2018-06-14 | Univ Johns Hopkins | Fremgangsmåde til dannelse af hypermutabel gær |
| US6569681B1 (en) * | 2000-03-14 | 2003-05-27 | Transkaryotic Therapies, Inc. | Methods of improving homologous recombination |
| US6596541B2 (en) * | 2000-10-31 | 2003-07-22 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| DE60144247D1 (de) | 2001-01-15 | 2011-04-28 | Morphotek Inc | Chemische hemmstoffe des mismatch-repair |
| EP1572935A4 (de) | 2002-02-21 | 2006-09-13 | Morphotek Inc | Verfahren zur herstellung hypermutierbarer zellen unter verwendung von pmsr-homologen |
-
2003
- 2003-01-17 AT AT03703842T patent/ATE548461T1/de active
- 2003-01-17 AU AU2003205173A patent/AU2003205173C1/en not_active Expired
- 2003-01-17 US US10/348,074 patent/US7638334B2/en not_active Expired - Lifetime
- 2003-01-17 CA CA2473741A patent/CA2473741C/en not_active Expired - Lifetime
- 2003-01-17 WO PCT/US2003/001361 patent/WO2003062435A1/en not_active Ceased
- 2003-01-17 EP EP03703842A patent/EP1474522B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003205173B2 (en) | 2008-05-22 |
| EP1474522A4 (de) | 2005-09-07 |
| US20030176386A1 (en) | 2003-09-18 |
| CA2473741A1 (en) | 2003-07-31 |
| US7638334B2 (en) | 2009-12-29 |
| EP1474522B1 (de) | 2012-03-07 |
| CA2473741C (en) | 2015-12-22 |
| AU2003205173C1 (en) | 2009-01-29 |
| WO2003062435A1 (en) | 2003-07-31 |
| EP1474522A1 (de) | 2004-11-10 |
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