AU2001226023A1 - A method for generating hypermutable plants - Google Patents

A method for generating hypermutable plants

Info

Publication number
AU2001226023A1
AU2001226023A1 AU2001226023A AU2602301A AU2001226023A1 AU 2001226023 A1 AU2001226023 A1 AU 2001226023A1 AU 2001226023 A AU2001226023 A AU 2001226023A AU 2602301 A AU2602301 A AU 2602301A AU 2001226023 A1 AU2001226023 A1 AU 2001226023A1
Authority
AU
Australia
Prior art keywords
plants
genes
plant
expression
mismatch repair
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001226023A
Inventor
Luigi Grasso
Kenneth Kinzler
Nicholas C. Nicolaides
Philip M. Sass
Bert Vogelstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of AU2001226023A1 publication Critical patent/AU2001226023A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology

Abstract

Blockade of mismatch repair in a plant can lead to hypermutation and a new genotype and/or phenotype. One approach used to generate hypermutable plants is through the expression of dominant negative alleles of mismatch repair genes in transgenic plants or derived cells. By introducing these genes into cells and transgenic plants, new cell lines and plant varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. Moreover, methods to inhibit the expression and activity of endogenous plant MMR genes and their encoded products are also useful to generate hypermutable plants.
AU2001226023A 2000-02-18 2000-12-28 A method for generating hypermutable plants Abandoned AU2001226023A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18333300P 2000-02-18 2000-02-18
US60183333 2000-02-18
PCT/US2000/035397 WO2001061012A1 (en) 2000-02-18 2000-12-28 A method for generating hypermutable plants

Publications (1)

Publication Number Publication Date
AU2001226023A1 true AU2001226023A1 (en) 2001-08-27

Family

ID=22672379

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001226023A Abandoned AU2001226023A1 (en) 2000-02-18 2000-12-28 A method for generating hypermutable plants

Country Status (7)

Country Link
US (3) US6900370B2 (en)
EP (1) EP1282708B1 (en)
AT (1) ATE399871T1 (en)
AU (1) AU2001226023A1 (en)
CA (1) CA2400664C (en)
DE (1) DE60039371D1 (en)
WO (1) WO2001061012A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2422487A1 (en) * 2000-09-18 2002-03-28 Pioneer Hi-Bred International, Inc. Rice mlh1 ortholog and uses thereof
US6737268B1 (en) * 2000-11-14 2004-05-18 Morphotek, Inc. Method for generating genetically altered antigens
WO2003031937A2 (en) * 2001-10-12 2003-04-17 Morphotek, Inc. Genetic hypermutability of plants for gene discovery and diagnosis
CA2473741C (en) 2002-01-18 2015-12-22 Morphotek, Inc. A method for generating engineered cells for locus specific gene regulation and analysis
JP2005531291A (en) * 2002-02-21 2005-10-20 モーフオテク・インコーポレーテツド Method for producing hypermutable cells using PMSR homologues
DE60335502D1 (en) * 2002-02-21 2011-02-03 Morphotek Inc Regulated vectors for controlling DNA hypermutability in eukaryotic cells
WO2003104451A2 (en) * 2002-06-06 2003-12-18 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Modifying dna recombination and repair
IL155137A0 (en) * 2003-03-27 2003-10-31 Yissum Res Dev Co A method for generating plant diversity
WO2006032504A2 (en) * 2004-09-22 2006-03-30 Vrije Universiteit Brussel Method for introducing genetic mutations into plant cells
EP2115127A4 (en) * 2007-01-17 2010-04-07 Life Technologies Corp Methods and compositions for improving the health of cells in culture
US20090075378A1 (en) * 2007-02-20 2009-03-19 Anaptysbio, Inc. Somatic hypermutation systems
EP2371951A4 (en) * 2008-11-27 2012-07-18 Riken NOVEL MutS PROTEIN AND METHOD OF USING SAME TO DETERMINE MUTATIONS
EP2376637A1 (en) * 2008-12-22 2011-10-19 Keygene N.V. Use of double stranded rna to increase the efficiency of targeted gene alteration in plant protoplasts
US20130023051A1 (en) * 2009-12-21 2013-01-24 Keygene N.V. Techniques for transfecting protoplasts
US20150307876A1 (en) * 2012-02-10 2015-10-29 Regents Of The University Of Minnesota Dna assimilation

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6191268B1 (en) * 1993-12-17 2001-02-20 Dana-Farber Cancer Institute Compositions and methods relating to DNA mismatch repair genes
CA2263958C (en) 1995-07-26 2012-06-19 Mixis France Homologous recombination in mismatch repair inactivated eukaryotic cells
US5843757A (en) 1995-08-24 1998-12-01 The Johns Hopkins University Human JTV1 gene overlaps PMS2 gene
US5907079A (en) * 1996-01-18 1999-05-25 Amgen Canada Inc. MSH2 disrupted mice develop lymphomas
US6326204B1 (en) * 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
AUPO974597A0 (en) * 1997-10-10 1997-11-06 Rhone-Poulenc Agro Methods for obtaining plant varieties
US6146894A (en) * 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms

Also Published As

Publication number Publication date
CA2400664A1 (en) 2001-08-23
US20020128460A1 (en) 2002-09-12
US6900370B2 (en) 2005-05-31
US20110055964A1 (en) 2011-03-03
EP1282708A1 (en) 2003-02-12
US7704689B2 (en) 2010-04-27
CA2400664C (en) 2011-03-01
ATE399871T1 (en) 2008-07-15
US8110356B2 (en) 2012-02-07
DE60039371D1 (en) 2008-08-14
EP1282708B1 (en) 2008-07-02
WO2001061012A1 (en) 2001-08-23
US20050266463A1 (en) 2005-12-01

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