ATE355367T1 - Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben - Google Patents

Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben

Info

Publication number
ATE355367T1
ATE355367T1 AT98948499T AT98948499T ATE355367T1 AT E355367 T1 ATE355367 T1 AT E355367T1 AT 98948499 T AT98948499 T AT 98948499T AT 98948499 T AT98948499 T AT 98948499T AT E355367 T1 ATE355367 T1 AT E355367T1
Authority
AT
Austria
Prior art keywords
nucleic acid
library
libraries
normalized
acid molecules
Prior art date
Application number
AT98948499T
Other languages
English (en)
Inventor
Wu-Bo Li
Joel Jessee
Paul E Nisson
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE355367T1 publication Critical patent/ATE355367T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/08—Methods of screening libraries by measuring catalytic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT98948499T 1997-09-24 1998-09-24 Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben ATE355367T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5981797P 1997-09-24 1997-09-24
US09/159,496 US6399334B1 (en) 1997-09-24 1998-09-23 Normalized nucleic acid libraries and methods of production thereof

Publications (1)

Publication Number Publication Date
ATE355367T1 true ATE355367T1 (de) 2006-03-15

Family

ID=26739222

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98948499T ATE355367T1 (de) 1997-09-24 1998-09-24 Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben

Country Status (9)

Country Link
US (4) US6399334B1 (de)
EP (1) EP1017853B1 (de)
JP (1) JP2001517460A (de)
AT (1) ATE355367T1 (de)
AU (1) AU9505998A (de)
CA (1) CA2304895A1 (de)
DE (1) DE69837208T2 (de)
ES (1) ES2281138T3 (de)
WO (1) WO1999015702A1 (de)

Families Citing this family (106)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5244797B1 (en) * 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
US8476011B1 (en) * 1996-06-18 2013-07-02 Bp Corporation North America Inc. Construction and use of catalogued nucleic acid libraries that contain advantageously adjusted representations of defined components
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
AU762888B2 (en) * 1997-02-12 2003-07-10 Us Genomics Methods and products for analyzing polymers
US6399334B1 (en) * 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6638717B2 (en) 1999-05-19 2003-10-28 Aventis Pharmaceuticals, Inc. Microarray-based subtractive hybridzation
US6589737B1 (en) * 1999-05-21 2003-07-08 Invitrogen Corporation Compositions and methods for labeling of nucleic acid molecules
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
EP1290149A4 (de) 2000-05-26 2004-03-17 Invitrogen Corp Thermostabile reverse transkriptase und deren anwendungen
JP2004512027A (ja) * 2000-08-07 2004-04-22 ジーンメッド・バイオテクノロジーズ・インコーポレーテッド 低存在量ポリヌクレオチド及び関連する組成物の同定方法
DE60124363T2 (de) * 2000-08-25 2007-09-06 Riken, Wako Methode zur Herstellung von genormten und/oder subtrahierten cDNA
JP2004229501A (ja) * 2000-10-30 2004-08-19 Nippon Shinyaku Co Ltd 臓器別又は組織別の平均化ｃＤＮＡライブラリーを用いて作製したＤＮＡアレイ
EP1354064A2 (de) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen
AU2002249116A1 (en) * 2001-01-11 2002-09-19 Lion Bioscience Ag Gene library for screening methods
US8353932B2 (en) 2005-09-30 2013-01-15 Jackson Roger P Polyaxial bone anchor assembly with one-piece closure, pressure insert and plastic elongate member
US10258382B2 (en) 2007-01-18 2019-04-16 Roger P. Jackson Rod-cord dynamic connection assemblies with slidable bone anchor attachment members along the cord
US7862587B2 (en) 2004-02-27 2011-01-04 Jackson Roger P Dynamic stabilization assemblies, tool set and method
US10729469B2 (en) 2006-01-09 2020-08-04 Roger P. Jackson Flexible spinal stabilization assembly with spacer having off-axis core member
US8292926B2 (en) 2005-09-30 2012-10-23 Jackson Roger P Dynamic stabilization connecting member with elastic core and outer sleeve
JP4634799B2 (ja) 2002-09-13 2011-02-16 ライフ テクノロジーズ コーポレーション 耐熱性逆転写酵素およびその使用法
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
JP4480715B2 (ja) 2003-01-29 2010-06-16 ４５４ コーポレーション 二重末端シーケンシング
US7621918B2 (en) 2004-11-23 2009-11-24 Jackson Roger P Spinal fixation tool set and method
US7766915B2 (en) 2004-02-27 2010-08-03 Jackson Roger P Dynamic fixation assemblies with inner core and outer coil-like member
US11419642B2 (en) 2003-12-16 2022-08-23 Medos International Sarl Percutaneous access devices and bone anchor assemblies
US7179261B2 (en) 2003-12-16 2007-02-20 Depuy Spine, Inc. Percutaneous access devices and bone anchor assemblies
US7527638B2 (en) 2003-12-16 2009-05-05 Depuy Spine, Inc. Methods and devices for minimally invasive spinal fixation element placement
US9050148B2 (en) * 2004-02-27 2015-06-09 Roger P. Jackson Spinal fixation tool attachment structure
US7789896B2 (en) * 2005-02-22 2010-09-07 Jackson Roger P Polyaxial bone screw assembly
US8152810B2 (en) 2004-11-23 2012-04-10 Jackson Roger P Spinal fixation tool set and method
US7160300B2 (en) 2004-02-27 2007-01-09 Jackson Roger P Orthopedic implant rod reduction tool set and method
AU2004317551B2 (en) 2004-02-27 2008-12-04 Roger P. Jackson Orthopedic implant rod reduction tool set and method
AU2005230684B2 (en) * 2004-04-05 2011-10-06 Alnylam Pharmaceuticals, Inc. Process and reagents for oligonucleotide synthesis and purification
ES2292270B1 (es) * 2004-04-14 2009-02-16 Oryzon Genomics, S.A. Procedimiento para detectar selectivamente acidos nucleicos con estructuras atipicas convertibles en muescas.
JP4584986B2 (ja) 2004-04-27 2010-11-24 アルニラム ファーマスーティカルズ インコーポレイテッド ２−アリールプロピル部分を含む１本鎖及び２本鎖オリゴヌクレオチド
WO2006073458A2 (en) * 2004-04-30 2006-07-13 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a c5-modified pyrimidine
AU2005327517B2 (en) 2004-06-30 2011-05-26 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a non-phosphate backbone linkage
AU2005328382C1 (en) 2004-07-21 2013-01-24 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a modified or non-natural nucleobase
CA2574603C (en) 2004-08-04 2014-11-04 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase
WO2006058221A2 (en) 2004-11-24 2006-06-01 Abdou Samy M Devices and methods for inter-vertebral orthopedic device placement
US8105368B2 (en) 2005-09-30 2012-01-31 Jackson Roger P Dynamic stabilization connecting member with slitted core and outer sleeve
US7704271B2 (en) 2005-12-19 2010-04-27 Abdou M Samy Devices and methods for inter-vertebral orthopedic device placement
US20080038237A1 (en) * 2006-02-28 2008-02-14 Sapolsky Robert M Trojan horse immunotherapy
EP2088945A4 (de) 2006-12-08 2010-02-17 Roger P Jackson Werkzeugsystem für dynamische wirbelsäulenimplantate
US8366745B2 (en) 2007-05-01 2013-02-05 Jackson Roger P Dynamic stabilization assembly having pre-compressed spacers with differential displacements
US10383660B2 (en) 2007-05-01 2019-08-20 Roger P. Jackson Soft stabilization assemblies with pretensioned cords
BR122021005861B1 (pt) 2008-12-03 2023-10-24 The Scripps Research Institute Composto, métodos para estabilizar uma célula animal isolada in vitro e para manter a sobrevivência da célula, e, composição
US8764806B2 (en) 2009-12-07 2014-07-01 Samy Abdou Devices and methods for minimally invasive spinal stabilization and instrumentation
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2012033532A1 (en) 2010-09-08 2012-03-15 Roger Jackson P Dynamic stabilization members with elastic and inelastic sections
USRE49975E1 (en) 2011-03-10 2024-05-21 Gen-Probe Incorporated Methods and compositions for the selection and optimization of oligonucleotide tag sequences
GB2497317A (en) * 2011-12-06 2013-06-12 Univ Dublin System and method for the detection of analytes using rod-shaped nanoparticles
US8845728B1 (en) 2011-09-23 2014-09-30 Samy Abdou Spinal fixation devices and methods of use
US20130226240A1 (en) 2012-02-22 2013-08-29 Samy Abdou Spinous process fixation devices and methods of use
EP3321378B1 (de) 2012-02-27 2021-11-10 Becton, Dickinson and Company Zusammensetzungen für molekularen zählung
US9198767B2 (en) 2012-08-28 2015-12-01 Samy Abdou Devices and methods for spinal stabilization and instrumentation
AU2013325107C1 (en) * 2012-09-25 2019-02-28 Agriculture Victoria Services Pty Ltd Method of producing a normalised nucleic acid library using solid state capture material
US9320617B2 (en) 2012-10-22 2016-04-26 Cogent Spine, LLC Devices and methods for spinal stabilization and instrumentation
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
JP2017504307A (ja) 2013-10-07 2017-02-09 セルラー リサーチ， インコーポレイテッド アレイ上のフィーチャーをデジタルカウントするための方法およびシステム
WO2016134078A1 (en) 2015-02-19 2016-08-25 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
ES2934982T3 (es) 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
EP3347465B1 (de) * 2015-09-11 2019-06-26 Cellular Research, Inc. Verfahren und zusammensetzung zur standardisierung von nukleinsäure bibliothek
US10857003B1 (en) 2015-10-14 2020-12-08 Samy Abdou Devices and methods for vertebral stabilization
JP7129343B2 (ja) 2016-05-02 2022-09-01 ベクトン・ディキンソン・アンド・カンパニー 正確な分子バーコーディング
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (de) 2016-05-26 2024-04-10 Becton, Dickinson and Company Verfahren zur anpassung des zählens von molekularen kennzeichen
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP3516400B1 (de) 2016-09-26 2023-08-16 Becton, Dickinson and Company Messung der proteinexpression mit reagenzien mit strichcodierten oligonukleotidsequenzen
US10973648B1 (en) 2016-10-25 2021-04-13 Samy Abdou Devices and methods for vertebral bone realignment
US10744000B1 (en) 2016-10-25 2020-08-18 Samy Abdou Devices and methods for vertebral bone realignment
EP3539035B1 (de) 2016-11-08 2024-04-17 Becton, Dickinson and Company Verfahren zur klassifizierung von expressionsprofilen
KR102790039B1 (ko) 2016-11-08 2025-04-04 벡톤 디킨슨 앤드 컴퍼니 세포 표지 분류 방법
CN110573253B (zh) 2017-01-13 2021-11-02 赛卢拉研究公司 流体通道的亲水涂层
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
WO2019126209A1 (en) 2017-12-19 2019-06-27 Cellular Research, Inc. Particles associated with oligonucleotides
JP7358388B2 (ja) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
ES2945191T3 (es) 2018-05-03 2023-06-29 Becton Dickinson Co Análisis de muestras multiómicas de alto rendimiento
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
US11179248B2 (en) 2018-10-02 2021-11-23 Samy Abdou Devices and methods for spinal implantation
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
FI3887540T3 (fi) * 2018-11-30 2023-09-14 Illumina Inc Useiden analyyttien analyysi käyttäen yhtä määritystä
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322B1 (de) 2019-01-23 2024-08-21 Becton, Dickinson and Company Mit antikörpern assoziierte oligonukleotide
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
CN115335520A (zh) 2020-01-29 2022-11-11 贝克顿迪金森公司 用于通过测序对单细胞进行空间映射的条形码化的孔
EP4111168A1 (de) 2020-02-25 2023-01-04 Becton Dickinson and Company Bispezifische sonden zur ermöglichung der verwendung von einzelzellenproben als einzelfarbkompensationssteuerung
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） 用于免疫组库谱分析的引物
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN116635533A (zh) 2020-11-20 2023-08-22 贝克顿迪金森公司 高表达的蛋白和低表达的蛋白的谱分析
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4888274A (en) * 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5618702A (en) 1988-08-19 1997-04-08 City Of Hope PCR amplification of mRNA
US5817797A (en) 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
US5681726A (en) 1988-09-19 1997-10-28 Stratagene Method of double stranded DNA synthesis
JP2960776B2 (ja) 1988-11-21 1999-10-12 ダイナル・エイ・エス ｃＤＮＡを製造する方法
US5759820A (en) 1988-11-21 1998-06-02 Dynal As Process for producing cDNA
CA2020729A1 (en) * 1989-07-19 1991-01-20 Michael C. Kiefer Bone morphogenetic protein
WO1991011533A1 (en) 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
GB9011454D0 (en) 1990-05-22 1990-07-11 Medical Res Council Polynucleotide amplification
US5326691A (en) 1991-11-21 1994-07-05 John Hozier Micro-libraries and methods of making and manipulating them methods for generating and analyzing micro-libraries
US5262311A (en) 1992-03-11 1993-11-16 Dana-Farber Cancer Institute, Inc. Methods to clone polyA mRNA
JP3315439B2 (ja) 1992-07-21 2002-08-19 住友電気工業株式会社 細胞特異的ｃＤＮＡの分離法及びライブラリー作製法
US5500356A (en) * 1993-08-10 1996-03-19 Life Technologies, Inc. Method of nucleic acid sequence selection
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
DE4332463A1 (de) 1993-09-24 1995-03-30 Boehringer Mannheim Gmbh Verfahren zur spezifischen Klonierung von Nukleinsäuren
US5643761A (en) 1993-10-27 1997-07-01 The Trustees Of Columbia University In The City Of New York Method for generating a subtracted cDNA library and uses of the generated library
DE69435103D1 (de) * 1993-10-27 2008-07-10 Univ Columbia Methode zur Bildung einer subtrahierten cDNA Bank und ihre Verwendung
US5484702A (en) * 1994-01-27 1996-01-16 Research Foundation Of The State University Of New York At Buffalo Method for preselecting recombinant clones containing a specific nucleic acid sequence and subsequent transformation with preselected clones
US5629179A (en) 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
US5707807A (en) 1995-03-28 1998-01-13 Research Development Corporation Of Japan Molecular indexing for expressed gene analysis
US5989813A (en) * 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
AU6643596A (en) 1995-08-11 1997-03-12 United States Of America, As Represented By The Secretary, Department Of Health And Human Services, The Isolation of amplified genes via cdna subtractive hybridization
US5866330A (en) 1995-09-12 1999-02-02 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
EP0872559A1 (de) 1995-11-09 1998-10-21 Shionogi & Co., Ltd. Verfahren zur gleichzeitigen erkennung von polymeren
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
AU2743097A (en) 1996-05-01 1997-11-19 Trustees Of The University Of Pennsylvania, The Simplified use of 5' ends of rnas for cloning and cdna library construction
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US5846721A (en) 1996-09-19 1998-12-08 The Trustees Of Columbia University In The City Of New York Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
AU4753397A (en) 1996-10-03 1998-04-24 Incyte Pharmaceuticals, Inc. Methods for generating and analyzing transcript markers
US5891637A (en) * 1996-10-15 1999-04-06 Genentech, Inc. Construction of full length cDNA libraries
US5726022A (en) 1996-11-18 1998-03-10 Lifespan Biosciences, Inc. Subtractive hybridization and capture methods and kits for differential isolation of nucleic acids including disease-associated sequences
US6060245A (en) * 1996-12-13 2000-05-09 Stratagene Methods and adaptors for generating specific nucleic acid populations
US6268133B1 (en) * 1997-06-25 2001-07-31 Invitrogen Corporation Method for isolating and recovering target DNA or RNA molecules having a desired nucleotide sequence
US6399334B1 (en) * 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
AU744699B2 (en) * 1998-03-04 2002-02-28 Verenium Corporation Production and use of normalized DNA libraries

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DE69837208T2 (de) 2007-11-22
US20020034790A1 (en) 2002-03-21
DE69837208D1 (de) 2007-04-12
AU9505998A (en) 1999-04-12
EP1017853A1 (de) 2000-07-12
US20090264304A1 (en) 2009-10-22
EP1017853A4 (de) 2004-11-17
CA2304895A1 (en) 1999-04-01
ES2281138T3 (es) 2007-09-16
US20060269940A1 (en) 2006-11-30
JP2001517460A (ja) 2001-10-09
WO1999015702A1 (en) 1999-04-01
EP1017853B1 (de) 2007-02-28
US6399334B1 (en) 2002-06-04
US20020177155A1 (en) 2002-11-28

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