DE69837208D1 - Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben - Google Patents

Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben

Info

Publication number
DE69837208D1
DE69837208D1 DE69837208T DE69837208T DE69837208D1 DE 69837208 D1 DE69837208 D1 DE 69837208D1 DE 69837208 T DE69837208 T DE 69837208T DE 69837208 T DE69837208 T DE 69837208T DE 69837208 D1 DE69837208 D1 DE 69837208D1
Authority
DE
Germany
Prior art keywords
nucleic acid
library
libraries
acid molecules
normalized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69837208T
Other languages
English (en)
Other versions
DE69837208T2 (de
Inventor
Wu-Bo Li
Joel Jessee
Paul E Nisson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of DE69837208D1 publication Critical patent/DE69837208D1/de
Publication of DE69837208T2 publication Critical patent/DE69837208T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/08—Methods of screening libraries by measuring catalytic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69837208T 1997-09-24 1998-09-24 Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben Expired - Lifetime DE69837208T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US5981797P 1997-09-24 1997-09-24
US59817P 1997-09-24
US09/159,496 US6399334B1 (en) 1997-09-24 1998-09-23 Normalized nucleic acid libraries and methods of production thereof
US159496 1998-09-23
PCT/US1998/019948 WO1999015702A1 (en) 1997-09-24 1998-09-24 Normalized nucleic acid libraries and methods of production thereof

Publications (2)

Publication Number Publication Date
DE69837208D1 true DE69837208D1 (de) 2007-04-12
DE69837208T2 DE69837208T2 (de) 2007-11-22

Family

ID=26739222

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69837208T Expired - Lifetime DE69837208T2 (de) 1997-09-24 1998-09-24 Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben

Country Status (9)

Country Link
US (4) US6399334B1 (de)
EP (1) EP1017853B1 (de)
JP (1) JP2001517460A (de)
AT (1) ATE355367T1 (de)
AU (1) AU9505998A (de)
CA (1) CA2304895A1 (de)
DE (1) DE69837208T2 (de)
ES (1) ES2281138T3 (de)
WO (1) WO1999015702A1 (de)

Families Citing this family (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5244797B1 (en) * 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
US8476011B1 (en) 1996-06-18 2013-07-02 Bp Corporation North America Inc. Construction and use of catalogued nucleic acid libraries that contain advantageously adjusted representations of defined components
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
JP3935509B2 (ja) * 1997-02-12 2007-06-27 ワイ． チャン，ユージーン ポリマー分析のための方法および製品
US6399334B1 (en) * 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6638717B2 (en) 1999-05-19 2003-10-28 Aventis Pharmaceuticals, Inc. Microarray-based subtractive hybridzation
WO2000071757A1 (en) * 1999-05-21 2000-11-30 Invitrogen Corporation Compositions and methods for labeling of nucleic acid molecules
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
JP2004511211A (ja) 2000-05-26 2004-04-15 インヴィトロジェン　コーポレーション 耐熱性逆転写酵素およびその使用
US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
WO2002012564A2 (en) * 2000-08-07 2002-02-14 Genemed Biotechnologies, Inc. Methods for identifying low-abundance polynucleotides and related compositions
DE60124363T2 (de) * 2000-08-25 2007-09-06 Riken, Wako Methode zur Herstellung von genormten und/oder subtrahierten cDNA
JP2004229501A (ja) * 2000-10-30 2004-08-19 Nippon Shinyaku Co Ltd 臓器別又は組織別の平均化ｃＤＮＡライブラリーを用いて作製したＤＮＡアレイ
AU2002227156A1 (en) * 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2002070684A2 (en) * 2001-01-11 2002-09-12 Lion Bioscience Ag Gene library for screening methods
US8292926B2 (en) 2005-09-30 2012-10-23 Jackson Roger P Dynamic stabilization connecting member with elastic core and outer sleeve
US10729469B2 (en) 2006-01-09 2020-08-04 Roger P. Jackson Flexible spinal stabilization assembly with spacer having off-axis core member
US7862587B2 (en) 2004-02-27 2011-01-04 Jackson Roger P Dynamic stabilization assemblies, tool set and method
US8353932B2 (en) 2005-09-30 2013-01-15 Jackson Roger P Polyaxial bone anchor assembly with one-piece closure, pressure insert and plastic elongate member
US10258382B2 (en) 2007-01-18 2019-04-16 Roger P. Jackson Rod-cord dynamic connection assemblies with slidable bone anchor attachment members along the cord
AU2003274973A1 (en) 2002-09-13 2004-04-30 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
US7621918B2 (en) 2004-11-23 2009-11-24 Jackson Roger P Spinal fixation tool set and method
US7766915B2 (en) 2004-02-27 2010-08-03 Jackson Roger P Dynamic fixation assemblies with inner core and outer coil-like member
US7527638B2 (en) 2003-12-16 2009-05-05 Depuy Spine, Inc. Methods and devices for minimally invasive spinal fixation element placement
US7179261B2 (en) 2003-12-16 2007-02-20 Depuy Spine, Inc. Percutaneous access devices and bone anchor assemblies
US11419642B2 (en) 2003-12-16 2022-08-23 Medos International Sarl Percutaneous access devices and bone anchor assemblies
US9050148B2 (en) * 2004-02-27 2015-06-09 Roger P. Jackson Spinal fixation tool attachment structure
US7789896B2 (en) * 2005-02-22 2010-09-07 Jackson Roger P Polyaxial bone screw assembly
US8152810B2 (en) 2004-11-23 2012-04-10 Jackson Roger P Spinal fixation tool set and method
US7160300B2 (en) 2004-02-27 2007-01-09 Jackson Roger P Orthopedic implant rod reduction tool set and method
WO2005092218A1 (en) 2004-02-27 2005-10-06 Jackson Roger P Orthopedic implant rod reduction tool set and method
EP2540734B1 (de) * 2004-04-05 2016-03-30 Alnylam Pharmaceuticals, Inc. Prozess und Reagenzien für Oligonukleotidsynthese und -reinigung
ES2292270B1 (es) * 2004-04-14 2009-02-16 Oryzon Genomics, S.A. Procedimiento para detectar selectivamente acidos nucleicos con estructuras atipicas convertibles en muescas.
EP1768998A2 (de) * 2004-04-27 2007-04-04 Alnylam Pharmaceuticals Inc. Einzelstrang- und doppelstrang-oligonukleotide mit 2-arylpropyl-anteil
EP3034510A1 (de) * 2004-04-30 2016-06-22 Alnylam Pharmaceuticals Inc. Oligonukleotide mit c5-modifiziertem pyrimidin
AU2005327517B2 (en) 2004-06-30 2011-05-26 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a non-phosphate backbone linkage
AU2005328382C1 (en) 2004-07-21 2013-01-24 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a modified or non-natural nucleobase
EP1913011B1 (de) 2004-08-04 2016-11-02 Alnylam Pharmaceuticals Inc. Oligonukleotide mit einem an einer modifizierten oder nicht-natürlichen nukleobase angebundenen liganden
EP1814474B1 (de) 2004-11-24 2011-09-14 Samy Abdou Vorrichtungen zur platzierung eines orthopädischen intervertebralen implantats
US8105368B2 (en) 2005-09-30 2012-01-31 Jackson Roger P Dynamic stabilization connecting member with slitted core and outer sleeve
US7704271B2 (en) 2005-12-19 2010-04-27 Abdou M Samy Devices and methods for inter-vertebral orthopedic device placement
WO2007100892A2 (en) * 2006-02-28 2007-09-07 The Board Of Trustees Of The Leland Stanford Junior University Trojan horse immunotherapy
CA2670988C (en) 2006-12-08 2014-03-25 Roger P. Jackson Tool system for dynamic spinal implants
US8366745B2 (en) 2007-05-01 2013-02-05 Jackson Roger P Dynamic stabilization assembly having pre-compressed spacers with differential displacements
US10383660B2 (en) 2007-05-01 2019-08-20 Roger P. Jackson Soft stabilization assemblies with pretensioned cords
ES2898303T3 (es) 2008-12-03 2022-03-07 Scripps Research Inst Cultivos de células madre
US8764806B2 (en) 2009-12-07 2014-07-01 Samy Abdou Devices and methods for minimally invasive spinal stabilization and instrumentation
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CA2810978A1 (en) 2010-09-08 2012-03-15 Roger P. Jackson Dynamic stabilization members with elastic and inelastic sections
USRE49975E1 (en) 2011-03-10 2024-05-21 Gen-Probe Incorporated Methods and compositions for the selection and optimization of oligonucleotide tag sequences
GB2497317A (en) * 2011-12-06 2013-06-12 Univ Dublin System and method for the detection of analytes using rod-shaped nanoparticles
US8845728B1 (en) 2011-09-23 2014-09-30 Samy Abdou Spinal fixation devices and methods of use
US20130226240A1 (en) 2012-02-22 2013-08-29 Samy Abdou Spinous process fixation devices and methods of use
GB2504240B (en) 2012-02-27 2015-05-27 Cellular Res Inc Compositions and kits for molecular counting of nucleic acids
US9198767B2 (en) 2012-08-28 2015-12-01 Samy Abdou Devices and methods for spinal stabilization and instrumentation
AU2013325107C1 (en) * 2012-09-25 2019-02-28 Agriculture Victoria Services Pty Ltd Method of producing a normalised nucleic acid library using solid state capture material
US9320617B2 (en) 2012-10-22 2016-04-26 Cogent Spine, LLC Devices and methods for spinal stabilization and instrumentation
GB2525104B (en) 2013-08-28 2016-09-28 Cellular Res Inc Massively Parallel Single Cell Nucleic Acid Analysis
JP2017504307A (ja) 2013-10-07 2017-02-09 セルラー リサーチ， インコーポレイテッド アレイ上のフィーチャーをデジタルカウントするための方法およびシステム
CN107250379B (zh) 2015-02-19 2021-12-28 贝克顿迪金森公司 结合蛋白质组信息和基因组信息的高通量单细胞分析
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
EP3835431B1 (de) 2015-03-30 2022-11-02 Becton, Dickinson and Company Verfahren für kombinatorische barcodierung
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
JP6940484B2 (ja) * 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド ライブラリー正規化のための方法および組成物
US10857003B1 (en) 2015-10-14 2020-12-08 Samy Abdou Devices and methods for vertebral stabilization
EP4269616A3 (de) 2016-05-02 2024-02-14 Becton, Dickinson and Company Genaue molekulare barcodierung
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
WO2017205691A1 (en) 2016-05-26 2017-11-30 Cellular Research, Inc. Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
ES2961743T3 (es) 2016-09-26 2024-03-13 Becton Dickinson Co Medición de la expresión de proteínas utilizando reactivos con secuencias de oligonucleótidos con código de barras
US10973648B1 (en) 2016-10-25 2021-04-13 Samy Abdou Devices and methods for vertebral bone realignment
US10744000B1 (en) 2016-10-25 2020-08-18 Samy Abdou Devices and methods for vertebral bone realignment
SG11201903139SA (en) 2016-11-08 2019-05-30 Cellular Res Inc Methods for expression profile classification
US11608497B2 (en) 2016-11-08 2023-03-21 Becton, Dickinson And Company Methods for cell label classification
EP3568234B1 (de) 2017-01-13 2023-09-06 Cellular Research, Inc. Hydrophile beschichtung von fluidischen kanälen
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
EP4345172A2 (de) 2017-06-05 2024-04-03 Becton, Dickinson and Company Probenindizierung für einzelzellen
US11946095B2 (en) 2017-12-19 2024-04-02 Becton, Dickinson And Company Particles associated with oligonucleotides
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170A1 (de) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molekulare barcodierung auf gegenüberliegenden transkriptenden
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
US11179248B2 (en) 2018-10-02 2021-11-23 Samy Abdou Devices and methods for spinal implantation
EP3877520A1 (de) 2018-11-08 2021-09-15 Becton Dickinson and Company Ganze transkriptomanalyse von einzelzellen mittels random-priming
ES2960890T3 (es) * 2018-11-30 2024-03-07 Illumina Inc Análisis de múltiples analitos utilizando un solo ensayo
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322A3 (de) 2019-01-23 2023-09-20 Becton, Dickinson and Company Mit antikörpern assoziierte oligonukleotide
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
EP4004231A1 (de) 2019-07-22 2022-06-01 Becton, Dickinson and Company Test für einzelzell-chromatin-immunpräzipitationssequenzierung
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
EP4090763A1 (de) 2020-01-13 2022-11-23 Becton Dickinson and Company Verfahren und zusammensetzungen zur quantifizierung von proteinen und rna
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4888274A (en) * 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5618702A (en) 1988-08-19 1997-04-08 City Of Hope PCR amplification of mRNA
US5817797A (en) 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
US5681726A (en) 1988-09-19 1997-10-28 Stratagene Method of double stranded DNA synthesis
US5759820A (en) 1988-11-21 1998-06-02 Dynal As Process for producing cDNA
WO1990006044A2 (en) 1988-11-21 1990-06-14 Dynal As PROCESS FOR PRODUCING cDNA
CA2020729A1 (en) * 1989-07-19 1991-01-20 Michael C. Kiefer Bone morphogenetic protein
WO1991011533A1 (en) 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
GB9011454D0 (en) 1990-05-22 1990-07-11 Medical Res Council Polynucleotide amplification
US5326691A (en) 1991-11-21 1994-07-05 John Hozier Micro-libraries and methods of making and manipulating them methods for generating and analyzing micro-libraries
US5262311A (en) 1992-03-11 1993-11-16 Dana-Farber Cancer Institute, Inc. Methods to clone polyA mRNA
JP3315439B2 (ja) 1992-07-21 2002-08-19 住友電気工業株式会社 細胞特異的ｃＤＮＡの分離法及びライブラリー作製法
US5500356A (en) * 1993-08-10 1996-03-19 Life Technologies, Inc. Method of nucleic acid sequence selection
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
DE4332463A1 (de) 1993-09-24 1995-03-30 Boehringer Mannheim Gmbh Verfahren zur spezifischen Klonierung von Nukleinsäuren
US5643761A (en) 1993-10-27 1997-07-01 The Trustees Of Columbia University In The City Of New York Method for generating a subtracted cDNA library and uses of the generated library
AU695540B2 (en) * 1993-10-27 1998-08-13 Trustees Of Columbia University In The City Of New York, The Method for generating a subtracted cDNA library and uses of the generated library
US5484702A (en) * 1994-01-27 1996-01-16 Research Foundation Of The State University Of New York At Buffalo Method for preselecting recombinant clones containing a specific nucleic acid sequence and subsequent transformation with preselected clones
US5629179A (en) 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
DE69621507T2 (de) 1995-03-28 2003-01-09 Japan Science & Tech Corp Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen
US5989813A (en) * 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
WO1997007244A1 (en) 1995-08-11 1997-02-27 The United States Of America, Represented By The ISOLATION OF AMPLIFIED GENES VIA cDNA SUBTRACTIVE HYBRIDIZATION
US5866330A (en) 1995-09-12 1999-02-02 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
EP0872559A1 (de) 1995-11-09 1998-10-21 Shionogi & Co., Ltd. Verfahren zur gleichzeitigen erkennung von polymeren
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US6623965B1 (en) 1996-05-01 2003-09-23 Trustees Of The University Of Pennsylvania Simplified use of 5′ ends of RNAs for cloning and cDNA library construction
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US5846721A (en) 1996-09-19 1998-12-08 The Trustees Of Columbia University In The City Of New York Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
WO1998014619A1 (en) 1996-10-03 1998-04-09 Incyte Pharmaceuticals, Inc. Methods for generating and analyzing transcript markers
US5891637A (en) * 1996-10-15 1999-04-06 Genentech, Inc. Construction of full length cDNA libraries
US5726022A (en) 1996-11-18 1998-03-10 Lifespan Biosciences, Inc. Subtractive hybridization and capture methods and kits for differential isolation of nucleic acids including disease-associated sequences
US6060245A (en) * 1996-12-13 2000-05-09 Stratagene Methods and adaptors for generating specific nucleic acid populations
EP1002126A4 (de) * 1997-06-25 2004-11-03 Life Technologies Inc Verbessertes verfahren zur isolierung und wiedergewinnung von ziel-dna oder -rna molekülen mit einer gewünschten nukleotidsequenz
US6399334B1 (en) * 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
AU744699B2 (en) * 1998-03-04 2002-02-28 Verenium Corporation Production and use of normalized DNA libraries

Also Published As

Publication number Publication date
US20090264304A1 (en) 2009-10-22
ES2281138T3 (es) 2007-09-16
ATE355367T1 (de) 2006-03-15
EP1017853A1 (de) 2000-07-12
CA2304895A1 (en) 1999-04-01
WO1999015702A1 (en) 1999-04-01
US20060269940A1 (en) 2006-11-30
US20020177155A1 (en) 2002-11-28
US20020034790A1 (en) 2002-03-21
JP2001517460A (ja) 2001-10-09
US6399334B1 (en) 2002-06-04
DE69837208T2 (de) 2007-11-22
EP1017853B1 (de) 2007-02-28
AU9505998A (en) 1999-04-12
EP1017853A4 (de) 2004-11-17

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