ATE286140T1 - E. coli extrakt zur synthese von proteinen - Google Patents
E. coli extrakt zur synthese von proteinenInfo
- Publication number
- ATE286140T1 ATE286140T1 AT01933021T AT01933021T ATE286140T1 AT E286140 T1 ATE286140 T1 AT E286140T1 AT 01933021 T AT01933021 T AT 01933021T AT 01933021 T AT01933021 T AT 01933021T AT E286140 T1 ATE286140 T1 AT E286140T1
- Authority
- AT
- Austria
- Prior art keywords
- protein synthesis
- extract
- reaction mixture
- proteins
- synthesis
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 241000588724 Escherichia coli Species 0.000 title abstract 2
- 230000015572 biosynthetic process Effects 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
- 238000001243 protein synthesis Methods 0.000 abstract 3
- 230000014616 translation Effects 0.000 abstract 3
- 238000006243 chemical reaction Methods 0.000 abstract 2
- 238000005194 fractionation Methods 0.000 abstract 2
- 239000011541 reaction mixture Substances 0.000 abstract 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000000153 supplemental effect Effects 0.000 abstract 1
- 238000010257 thawing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20145000P | 2000-05-03 | 2000-05-03 | |
| PCT/US2001/014469 WO2001083805A2 (en) | 2000-05-03 | 2001-05-03 | Improved e. coli extract for protein synthesis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE286140T1 true ATE286140T1 (de) | 2005-01-15 |
Family
ID=22745862
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01933021T ATE286140T1 (de) | 2000-05-03 | 2001-05-03 | E. coli extrakt zur synthese von proteinen |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7008651B2 (de) |
| EP (1) | EP1278883B1 (de) |
| AT (1) | ATE286140T1 (de) |
| AU (1) | AU2001259487A1 (de) |
| CA (1) | CA2400755A1 (de) |
| DE (1) | DE60108102T2 (de) |
| WO (1) | WO2001083805A2 (de) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6596747B2 (en) * | 1998-10-29 | 2003-07-22 | Bristol-Myers Squibb Company | Compounds derived from an amine nucleus and pharmaceutical compositions comprising same |
| JP4441170B2 (ja) | 2002-11-28 | 2010-03-31 | 独立行政法人理化学研究所 | Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法 |
| JP4477923B2 (ja) | 2003-05-22 | 2010-06-09 | 独立行政法人理化学研究所 | 無細胞タンパク質合成用抽出液の製造方法及びその方法により製造される細胞抽出液 |
| WO2004111203A1 (ja) * | 2003-06-10 | 2004-12-23 | Shimadzu Corporation | 哺乳動物培養細胞抽出液およびその調製方法、ならびに該抽出液を用いた無細胞系タンパク質合成方法 |
| WO2006026248A1 (en) | 2004-08-25 | 2006-03-09 | Sigma-Aldrich Co. | Compositions and methods employing zwitterionic detergent combinations |
| JP4868731B2 (ja) | 2004-11-17 | 2012-02-01 | 独立行政法人理化学研究所 | 哺乳動物培養細胞由来の無細胞タンパク質合成システム |
| JP4787488B2 (ja) | 2004-11-19 | 2011-10-05 | 独立行政法人理化学研究所 | 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液 |
| US7399620B2 (en) * | 2006-03-15 | 2008-07-15 | Sigma-Aldrich Co. | Polypeptides and bacterial strains for increased protein production |
| WO2008024427A2 (en) * | 2006-08-23 | 2008-02-28 | Science & Technology Corporation @ Unm | A virus-like platform for rapid vaccine discovery |
| EP2213747B1 (de) | 2007-11-05 | 2021-06-30 | Riken | Verfahren zur herstellung eines membranproteins |
| BR112012016305A2 (pt) | 2009-12-31 | 2017-05-02 | Stc Unm | "plasmídeos e métodos para exibição de peptídeo e seleção de afinidade em partículas semelhantes a vírus de bacteriófagos de rna" |
| US9717783B2 (en) | 2010-02-09 | 2017-08-01 | Stc.Unm | Immunogenic HPV L2-containing VLPs and related compositions, constructs, and therapeutic methods |
| CA3032514C (en) | 2015-07-31 | 2021-05-04 | Takehiro SHINODA | Method of producing membrane protein and utilization thereof |
| WO2019094859A1 (en) | 2017-11-10 | 2019-05-16 | Northwestern University | Cell-free protein synthesis platform derived from cellular extracts of vibrio natriegens |
| US11725224B2 (en) | 2018-04-16 | 2023-08-15 | Northwestern University | Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates |
| US11814621B2 (en) | 2018-06-01 | 2023-11-14 | Northwestern University | Expanding the chemical substrates for genetic code reprogramming |
| US12421537B2 (en) | 2018-07-06 | 2025-09-23 | Northwestern University | Ribosome variants for sequence defined polymer synthesis |
| WO2020072127A2 (en) | 2018-08-03 | 2020-04-09 | Northwestern University | On demand, portable, cell-free molecular sensing platform |
| CN113490748A (zh) | 2019-02-25 | 2021-10-08 | 西北大学 | 来源于梭菌属提取物的无细胞蛋白质合成平台 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69216385T2 (de) * | 1991-10-11 | 1997-06-12 | Promega Corp | Gekoppelte transkription und translation in zellfreien eukaryoten-extrakten |
| ATE357513T1 (de) * | 1996-07-16 | 2007-04-15 | Qiagen North American Holdings | Verfahren zur herstellung komplexer multienzymatischer lagerstabiler reaktionsgemische und deren verwendung |
| FR2782094B1 (fr) * | 1998-08-07 | 2002-03-01 | Centre Nat Rech Scient | Souches mutantes de e. coli et leur utilisation pour la production de polypeptides recombinants |
-
2001
- 2001-05-03 AT AT01933021T patent/ATE286140T1/de not_active IP Right Cessation
- 2001-05-03 AU AU2001259487A patent/AU2001259487A1/en not_active Abandoned
- 2001-05-03 DE DE60108102T patent/DE60108102T2/de not_active Expired - Fee Related
- 2001-05-03 WO PCT/US2001/014469 patent/WO2001083805A2/en not_active Ceased
- 2001-05-03 EP EP01933021A patent/EP1278883B1/de not_active Expired - Lifetime
- 2001-05-03 US US09/848,449 patent/US7008651B2/en not_active Expired - Fee Related
- 2001-05-03 CA CA002400755A patent/CA2400755A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20020034559A1 (en) | 2002-03-21 |
| AU2001259487A1 (en) | 2001-11-12 |
| WO2001083805A2 (en) | 2001-11-08 |
| CA2400755A1 (en) | 2001-11-08 |
| DE60108102D1 (de) | 2005-02-03 |
| US7008651B2 (en) | 2006-03-07 |
| EP1278883B1 (de) | 2004-12-29 |
| EP1278883A2 (de) | 2003-01-29 |
| WO2001083805A3 (en) | 2002-02-07 |
| DE60108102T2 (de) | 2005-12-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |