ATE286140T1 - E. coli extrakt zur synthese von proteinen - Google Patents

E. coli extrakt zur synthese von proteinen

Info

Publication number
ATE286140T1
ATE286140T1 AT01933021T AT01933021T ATE286140T1 AT E286140 T1 ATE286140 T1 AT E286140T1 AT 01933021 T AT01933021 T AT 01933021T AT 01933021 T AT01933021 T AT 01933021T AT E286140 T1 ATE286140 T1 AT E286140T1
Authority
AT
Austria
Prior art keywords
protein synthesis
extract
reaction mixture
proteins
synthesis
Prior art date
Application number
AT01933021T
Other languages
English (en)
Inventor
Yuping Ambuel
Oosbree Thomas R Van
Mark R Mccormick
Robert M Mierendorf
Original Assignee
Emd Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Emd Biosciences Inc filed Critical Emd Biosciences Inc
Application granted granted Critical
Publication of ATE286140T1 publication Critical patent/ATE286140T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT01933021T 2000-05-03 2001-05-03 E. coli extrakt zur synthese von proteinen ATE286140T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20145000P 2000-05-03 2000-05-03
PCT/US2001/014469 WO2001083805A2 (en) 2000-05-03 2001-05-03 Improved e. coli extract for protein synthesis

Publications (1)

Publication Number Publication Date
ATE286140T1 true ATE286140T1 (de) 2005-01-15

Family

ID=22745862

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01933021T ATE286140T1 (de) 2000-05-03 2001-05-03 E. coli extrakt zur synthese von proteinen

Country Status (7)

Country Link
US (1) US7008651B2 (de)
EP (1) EP1278883B1 (de)
AT (1) ATE286140T1 (de)
AU (1) AU2001259487A1 (de)
CA (1) CA2400755A1 (de)
DE (1) DE60108102T2 (de)
WO (1) WO2001083805A2 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6596747B2 (en) * 1998-10-29 2003-07-22 Bristol-Myers Squibb Company Compounds derived from an amine nucleus and pharmaceutical compositions comprising same
JP4441170B2 (ja) 2002-11-28 2010-03-31 独立行政法人理化学研究所 Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
JP4477923B2 (ja) 2003-05-22 2010-06-09 独立行政法人理化学研究所 無細胞タンパク質合成用抽出液の製造方法及びその方法により製造される細胞抽出液
US20060141559A1 (en) * 2003-06-10 2006-06-29 Takashi Suzuki Extract from cultured mammalian cell, process for preparation thereof and method of cell-free protein synthesis using the extract
WO2006026248A1 (en) 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions and methods employing zwitterionic detergent combinations
JP4868731B2 (ja) 2004-11-17 2012-02-01 独立行政法人理化学研究所 哺乳動物培養細胞由来の無細胞タンパク質合成システム
JP4787488B2 (ja) 2004-11-19 2011-10-05 独立行政法人理化学研究所 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液
US7399620B2 (en) * 2006-03-15 2008-07-15 Sigma-Aldrich Co. Polypeptides and bacterial strains for increased protein production
US9803189B2 (en) * 2006-08-23 2017-10-31 Stc.Unm Virus-like platform for rapid vaccine discovery
US8226976B2 (en) 2007-11-05 2012-07-24 Riken Method of using cell-free protein synthesis to produce a membrane protein
CN102858960B (zh) 2009-12-31 2014-11-26 Stc.Unm公司 用于在rna噬菌体的病毒样颗粒上进行肽展示和亲和选择的质粒和方法
US9717783B2 (en) 2010-02-09 2017-08-01 Stc.Unm Immunogenic HPV L2-containing VLPs and related compositions, constructs, and therapeutic methods
JP6722951B2 (ja) 2015-07-31 2020-07-15 国立研究開発法人理化学研究所 膜タンパク質の製造方法およびその利用
US11673921B2 (en) 2017-11-10 2023-06-13 Northwestern University Cell-free protein synthesis platform derived from cellular extracts of Vibrio natriegens
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
US11814621B2 (en) 2018-06-01 2023-11-14 Northwestern University Expanding the chemical substrates for genetic code reprogramming

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993007287A1 (en) * 1991-10-11 1993-04-15 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
ATE357513T1 (de) * 1996-07-16 2007-04-15 Qiagen North American Holdings Verfahren zur herstellung komplexer multienzymatischer lagerstabiler reaktionsgemische und deren verwendung
FR2782094B1 (fr) * 1998-08-07 2002-03-01 Centre Nat Rech Scient Souches mutantes de e. coli et leur utilisation pour la production de polypeptides recombinants

Also Published As

Publication number Publication date
EP1278883A2 (de) 2003-01-29
WO2001083805A3 (en) 2002-02-07
AU2001259487A1 (en) 2001-11-12
EP1278883B1 (de) 2004-12-29
CA2400755A1 (en) 2001-11-08
US7008651B2 (en) 2006-03-07
DE60108102T2 (de) 2005-12-15
WO2001083805A2 (en) 2001-11-08
DE60108102D1 (de) 2005-02-03
US20020034559A1 (en) 2002-03-21

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