DE60108102D1 - E. coli extrakt zur synthese von proteinen - Google Patents
E. coli extrakt zur synthese von proteinenInfo
- Publication number
- DE60108102D1 DE60108102D1 DE60108102T DE60108102T DE60108102D1 DE 60108102 D1 DE60108102 D1 DE 60108102D1 DE 60108102 T DE60108102 T DE 60108102T DE 60108102 T DE60108102 T DE 60108102T DE 60108102 D1 DE60108102 D1 DE 60108102D1
- Authority
- DE
- Germany
- Prior art keywords
- protein synthesis
- extract
- reaction mixture
- proteins
- synthesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US20145000P | 2000-05-03 | 2000-05-03 | |
US201450P | 2000-05-03 | ||
PCT/US2001/014469 WO2001083805A2 (en) | 2000-05-03 | 2001-05-03 | Improved e. coli extract for protein synthesis |
Publications (2)
Publication Number | Publication Date |
---|---|
DE60108102D1 true DE60108102D1 (de) | 2005-02-03 |
DE60108102T2 DE60108102T2 (de) | 2005-12-15 |
Family
ID=22745862
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60108102T Expired - Fee Related DE60108102T2 (de) | 2000-05-03 | 2001-05-03 | E. coli extrakt zur synthese von proteinen |
Country Status (7)
Country | Link |
---|---|
US (1) | US7008651B2 (de) |
EP (1) | EP1278883B1 (de) |
AT (1) | ATE286140T1 (de) |
AU (1) | AU2001259487A1 (de) |
CA (1) | CA2400755A1 (de) |
DE (1) | DE60108102T2 (de) |
WO (1) | WO2001083805A2 (de) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6596747B2 (en) * | 1998-10-29 | 2003-07-22 | Bristol-Myers Squibb Company | Compounds derived from an amine nucleus and pharmaceutical compositions comprising same |
JP4441170B2 (ja) | 2002-11-28 | 2010-03-31 | 独立行政法人理化学研究所 | S12リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法 |
JP4477923B2 (ja) | 2003-05-22 | 2010-06-09 | 独立行政法人理化学研究所 | 無細胞タンパク質合成用抽出液の製造方法及びその方法により製造される細胞抽出液 |
US20060141559A1 (en) * | 2003-06-10 | 2006-06-29 | Takashi Suzuki | Extract from cultured mammalian cell, process for preparation thereof and method of cell-free protein synthesis using the extract |
WO2006026248A1 (en) | 2004-08-25 | 2006-03-09 | Sigma-Aldrich Co. | Compositions and methods employing zwitterionic detergent combinations |
JP4868731B2 (ja) | 2004-11-17 | 2012-02-01 | 独立行政法人理化学研究所 | 哺乳動物培養細胞由来の無細胞タンパク質合成システム |
JP4787488B2 (ja) | 2004-11-19 | 2011-10-05 | 独立行政法人理化学研究所 | 直鎖状鋳型dnaを用いた無細胞タンパク質合成方法及びそのための細胞抽出液 |
US7399620B2 (en) * | 2006-03-15 | 2008-07-15 | Sigma-Aldrich Co. | Polypeptides and bacterial strains for increased protein production |
US9803189B2 (en) * | 2006-08-23 | 2017-10-31 | Stc.Unm | Virus-like platform for rapid vaccine discovery |
DK2213747T3 (da) | 2007-11-05 | 2021-08-09 | Riken | Fremgangsmåde til fremstilling af membranprotein |
WO2011082381A2 (en) | 2009-12-31 | 2011-07-07 | Stc.Unm | Plasmids and methods for peptide display and affinity-selection on virus-like particles of rna bacteriophages |
US9717783B2 (en) | 2010-02-09 | 2017-08-01 | Stc.Unm | Immunogenic HPV L2-containing VLPs and related compositions, constructs, and therapeutic methods |
CA3032514C (en) | 2015-07-31 | 2021-05-04 | Takehiro SHINODA | Method of producing membrane protein and utilization thereof |
US11673921B2 (en) | 2017-11-10 | 2023-06-13 | Northwestern University | Cell-free protein synthesis platform derived from cellular extracts of Vibrio natriegens |
WO2019204346A1 (en) | 2018-04-16 | 2019-10-24 | Northwestern University | METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES |
US11814621B2 (en) | 2018-06-01 | 2023-11-14 | Northwestern University | Expanding the chemical substrates for genetic code reprogramming |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69216385T2 (de) * | 1991-10-11 | 1997-06-12 | Promega Corp | Gekoppelte transkription und translation in zellfreien eukaryoten-extrakten |
JP3916257B2 (ja) * | 1996-07-16 | 2007-05-16 | キアゲン ノース アメリカン ホールディングス インコーポレイテッド | コンプレックス多酵素貯蔵安定性反応混合物の製造方法とその使用方法 |
FR2782094B1 (fr) * | 1998-08-07 | 2002-03-01 | Centre Nat Rech Scient | Souches mutantes de e. coli et leur utilisation pour la production de polypeptides recombinants |
-
2001
- 2001-05-03 AT AT01933021T patent/ATE286140T1/de not_active IP Right Cessation
- 2001-05-03 DE DE60108102T patent/DE60108102T2/de not_active Expired - Fee Related
- 2001-05-03 EP EP01933021A patent/EP1278883B1/de not_active Expired - Lifetime
- 2001-05-03 US US09/848,449 patent/US7008651B2/en not_active Expired - Fee Related
- 2001-05-03 CA CA002400755A patent/CA2400755A1/en not_active Abandoned
- 2001-05-03 WO PCT/US2001/014469 patent/WO2001083805A2/en active IP Right Grant
- 2001-05-03 AU AU2001259487A patent/AU2001259487A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1278883A2 (de) | 2003-01-29 |
AU2001259487A1 (en) | 2001-11-12 |
CA2400755A1 (en) | 2001-11-08 |
EP1278883B1 (de) | 2004-12-29 |
WO2001083805A2 (en) | 2001-11-08 |
WO2001083805A3 (en) | 2002-02-07 |
US20020034559A1 (en) | 2002-03-21 |
US7008651B2 (en) | 2006-03-07 |
DE60108102T2 (de) | 2005-12-15 |
ATE286140T1 (de) | 2005-01-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |