AU2001259487A1 - Improved e. coli extract for protein synthesis - Google Patents
Improved e. coli extract for protein synthesisInfo
- Publication number
- AU2001259487A1 AU2001259487A1 AU2001259487A AU5948701A AU2001259487A1 AU 2001259487 A1 AU2001259487 A1 AU 2001259487A1 AU 2001259487 A AU2001259487 A AU 2001259487A AU 5948701 A AU5948701 A AU 5948701A AU 2001259487 A1 AU2001259487 A1 AU 2001259487A1
- Authority
- AU
- Australia
- Prior art keywords
- protein synthesis
- extract
- reaction mixture
- improved
- coli extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
There is now a market for kits containing the necessary components for performing protein synthesis reactions using template DNA of the experimenter. One category of existing system for performing protein synthesis reactions is based on an S-30 extract from the bacteria E. coli. It is disclosed here that a simple fractionation process can dramatically improve the performance of an S-30 prokaryotic protein synthesis reaction mixture. In one embodiment, the fractionation is a simple freezing and thawing of an S-30 extract combined with a supplemental mix, followed by centrifugation. The resulting fractionated S-30 reaction mixture yields more full-length target protein and less non-full length or non-target protein than possible using prior art S-30 systems.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US20145000P | 2000-05-03 | 2000-05-03 | |
US60201450 | 2000-05-03 | ||
PCT/US2001/014469 WO2001083805A2 (en) | 2000-05-03 | 2001-05-03 | Improved e. coli extract for protein synthesis |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001259487A1 true AU2001259487A1 (en) | 2001-11-12 |
Family
ID=22745862
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001259487A Abandoned AU2001259487A1 (en) | 2000-05-03 | 2001-05-03 | Improved e. coli extract for protein synthesis |
Country Status (7)
Country | Link |
---|---|
US (1) | US7008651B2 (en) |
EP (1) | EP1278883B1 (en) |
AT (1) | ATE286140T1 (en) |
AU (1) | AU2001259487A1 (en) |
CA (1) | CA2400755A1 (en) |
DE (1) | DE60108102T2 (en) |
WO (1) | WO2001083805A2 (en) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6596747B2 (en) * | 1998-10-29 | 2003-07-22 | Bristol-Myers Squibb Company | Compounds derived from an amine nucleus and pharmaceutical compositions comprising same |
JP4441170B2 (en) | 2002-11-28 | 2010-03-31 | 独立行政法人理化学研究所 | Escherichia coli cell extract having mutation in S12 ribosomal protein and cell-free protein production method using the same |
JP4477923B2 (en) | 2003-05-22 | 2010-06-09 | 独立行政法人理化学研究所 | Method for producing extract for cell-free protein synthesis and cell extract produced by the method |
KR20060019589A (en) * | 2003-06-10 | 2006-03-03 | 가부시키가이샤 시마즈세이사쿠쇼 | Extract from cultured mammalian cell, process for preparation thereof and method of cell-free protein synthesis using the extract |
WO2006026248A1 (en) | 2004-08-25 | 2006-03-09 | Sigma-Aldrich Co. | Compositions and methods employing zwitterionic detergent combinations |
JP4868731B2 (en) | 2004-11-17 | 2012-02-01 | 独立行政法人理化学研究所 | Cell-free protein synthesis system derived from cultured mammalian cells |
JP4787488B2 (en) | 2004-11-19 | 2011-10-05 | 独立行政法人理化学研究所 | Cell-free protein synthesis method using linear template DNA and cell extract therefor |
US7399620B2 (en) * | 2006-03-15 | 2008-07-15 | Sigma-Aldrich Co. | Polypeptides and bacterial strains for increased protein production |
US9803189B2 (en) * | 2006-08-23 | 2017-10-31 | Stc.Unm | Virus-like platform for rapid vaccine discovery |
DK2213747T3 (en) | 2007-11-05 | 2021-08-09 | Riken | METHOD OF MANUFACTURE OF MEMBRANE PROTEIN |
EP2519627B1 (en) | 2009-12-31 | 2016-03-30 | Stc.Unm | Plasmids and methods for peptide display and affinity-selection on virus-like particles of rna bacteriophages |
US9717783B2 (en) | 2010-02-09 | 2017-08-01 | Stc.Unm | Immunogenic HPV L2-containing VLPs and related compositions, constructs, and therapeutic methods |
WO2017022696A1 (en) | 2015-07-31 | 2017-02-09 | 国立研究開発法人理化学研究所 | Method of manufacturing membrane protein and utilization thereof |
US11673921B2 (en) | 2017-11-10 | 2023-06-13 | Northwestern University | Cell-free protein synthesis platform derived from cellular extracts of Vibrio natriegens |
US11725224B2 (en) | 2018-04-16 | 2023-08-15 | Northwestern University | Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates |
US11814621B2 (en) | 2018-06-01 | 2023-11-14 | Northwestern University | Expanding the chemical substrates for genetic code reprogramming |
WO2020072127A2 (en) | 2018-08-03 | 2020-04-09 | Northwestern University | On demand, portable, cell-free molecular sensing platform |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2904583B2 (en) * | 1991-10-11 | 1999-06-14 | プロメガ・コーポレーション | Coupling transcription and translation in eukaryotic cell-free extracts |
ATE357513T1 (en) * | 1996-07-16 | 2007-04-15 | Qiagen North American Holdings | METHOD FOR PRODUCING COMPLEX MULTIENZYMATIC STORAGE-STABLE REACTION MIXTURES AND THEIR USE |
FR2782094B1 (en) * | 1998-08-07 | 2002-03-01 | Centre Nat Rech Scient | MUTANT STRAINS OF E. COLI AND THEIR USE IN THE PRODUCTION OF RECOMBINANT POLYPEPTIDES |
-
2001
- 2001-05-03 AT AT01933021T patent/ATE286140T1/en not_active IP Right Cessation
- 2001-05-03 DE DE60108102T patent/DE60108102T2/en not_active Expired - Fee Related
- 2001-05-03 AU AU2001259487A patent/AU2001259487A1/en not_active Abandoned
- 2001-05-03 US US09/848,449 patent/US7008651B2/en not_active Expired - Fee Related
- 2001-05-03 WO PCT/US2001/014469 patent/WO2001083805A2/en active IP Right Grant
- 2001-05-03 CA CA002400755A patent/CA2400755A1/en not_active Abandoned
- 2001-05-03 EP EP01933021A patent/EP1278883B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US7008651B2 (en) | 2006-03-07 |
EP1278883B1 (en) | 2004-12-29 |
EP1278883A2 (en) | 2003-01-29 |
US20020034559A1 (en) | 2002-03-21 |
ATE286140T1 (en) | 2005-01-15 |
CA2400755A1 (en) | 2001-11-08 |
WO2001083805A3 (en) | 2002-02-07 |
DE60108102D1 (en) | 2005-02-03 |
DE60108102T2 (en) | 2005-12-15 |
WO2001083805A2 (en) | 2001-11-08 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2001259487A1 (en) | Improved e. coli extract for protein synthesis | |
Roberts et al. | RNA-peptide fusions for the in vitro selection of peptides and proteins | |
WO2003010328A3 (en) | Complexity management of genomic dna | |
AU5794498A (en) | Releasable nonvolatile mass-label molecules | |
AU2247397A (en) | Methods and compositions for determining the sequence of nucleic acid molecules | |
DE69636866D1 (en) | Reverse "two-hybrid"-systeme | |
AU2001294541A1 (en) | Mammalian genes; related reagents and methods | |
DE60236641D1 (en) | RECOMBINANT VIRAL SWITCHES FOR CONTROLLING GENE EXPRESSION IN PLANTS | |
WO1999036516A3 (en) | Method of determining the function of nucleotide sequences and the proteins they encode by transfecting the same into a host | |
WO2007021944A3 (en) | In vitro recombination method | |
NZ296748A (en) | Peripheral nervous system specific sodium channel proteins, DNA coding therefor, compositions and methods | |
WO2002084250A3 (en) | Methods for mass spectrometry detection and quantification of specific target proteins in complex biological samples | |
EP1075513B8 (en) | Continuous in vitro evolution | |
AU2434195A (en) | Thymidine kinase mutants | |
AU6975694A (en) | Salmonella identification by the polymerase chain reaction | |
WO2004005221A3 (en) | Benzoate- and anthranilate-inducible promoters | |
WO2004056986A3 (en) | Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors | |
ZA963971B (en) | A method and nucleotide sequence for transforming microorganisms | |
Dube | Recognition of tRNA by the ribosome A possible role of 5 S RNA | |
WO1998023750A3 (en) | Mammalian chemokines | |
WO2003020005A3 (en) | Lp mammalian proteins; related reagents | |
Vold et al. | Lysine tRNAs from Bacillus subtilis 168: structural analysis | |
WO2002074906A3 (en) | Lp mammalian proteins; related reagents | |
EP0751226A3 (en) | Process for amplifying nucleic acid sequences | |
NZ512332A (en) | Potassium channel interactors and uses therefor |