ATE237352T1 - Helfervirus-freie herstellung von aav - Google Patents
Helfervirus-freie herstellung von aavInfo
- Publication number
- ATE237352T1 ATE237352T1 AT96918295T AT96918295T ATE237352T1 AT E237352 T1 ATE237352 T1 AT E237352T1 AT 96918295 T AT96918295 T AT 96918295T AT 96918295 T AT96918295 T AT 96918295T AT E237352 T1 ATE237352 T1 AT E237352T1
- Authority
- AT
- Austria
- Prior art keywords
- helper virus
- aav
- free production
- virus
- production
- Prior art date
Links
- 241000700605 Viruses Species 0.000 abstract 3
- 241000702421 Dependoparvovirus Species 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000007796 conventional method Methods 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 238000001890 transfection Methods 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US48375795A | 1995-06-07 | 1995-06-07 | |
PCT/US1996/009405 WO1996040240A1 (en) | 1995-06-07 | 1996-06-06 | Helper virus-free aav production |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE237352T1 true ATE237352T1 (de) | 2003-05-15 |
Family
ID=23921407
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT96918295T ATE237352T1 (de) | 1995-06-07 | 1996-06-06 | Helfervirus-freie herstellung von aav |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0871483B1 (de) |
JP (1) | JP4108746B2 (de) |
AT (1) | ATE237352T1 (de) |
AU (1) | AU707862B2 (de) |
CA (1) | CA2221293C (de) |
DE (1) | DE69627532T2 (de) |
WO (1) | WO1996040240A1 (de) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2317646T3 (es) * | 1995-09-08 | 2009-04-16 | Genzyme Corporation | Vectores aav mejorados para terapia genica. |
US6004797A (en) | 1995-11-09 | 1999-12-21 | Avigen, Inc. | Adenovirus helper-free recombinant AAV Virion production |
US6221646B1 (en) | 1997-07-31 | 2001-04-24 | Chiron Corporation | Materials and methods for simplified AAV production |
CA2303768C (en) * | 1997-09-19 | 2009-11-24 | The Trustees Of The University Of Pennsylvania | Methods and vector constructs useful for production of recombinant aav |
PT1064393E (pt) * | 1998-03-20 | 2005-04-29 | Univ Pennsylvania | Composicoes e metodos para a producao de virus adeno-associados recombinantes sem auxiliar |
US6953690B1 (en) | 1998-03-20 | 2005-10-11 | The Trustees Of The University Of Pennsylvania | Compositions and methods for helper-free production of recombinant adeno-associated viruses |
AU764130B2 (en) * | 1998-10-27 | 2003-08-14 | Crucell Holland B.V. | Improved AAV vector production |
JP4693244B2 (ja) | 1999-03-18 | 2011-06-01 | ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア | 組換えアデノ随伴ウイルスのヘルパー無しの生産のための組成物および方法 |
US6258595B1 (en) | 1999-03-18 | 2001-07-10 | The Trustees Of The University Of Pennsylvania | Compositions and methods for helper-free production of recombinant adeno-associated viruses |
WO2001083797A2 (en) | 2000-04-28 | 2001-11-08 | Avigen, Inc. | Polynucleotides for use in recombinant adeno-associated virus virion production |
SG10201702685WA (en) | 2012-10-03 | 2017-04-27 | Glaxosmithkline Biolog Sa | Immunogenic composition |
JP6767483B2 (ja) | 2015-11-05 | 2020-10-14 | バンブー セラペウティクス インク | 遺伝子療法のための、改変されたフリードライヒ運動失調症遺伝子およびベクター |
CA2971303A1 (en) | 2016-06-21 | 2017-12-21 | Bamboo Therapeutics, Inc. | Optimized mini-dystrophin genes and expression cassettes and their use |
US20230241248A1 (en) | 2019-06-27 | 2023-08-03 | Pfizer Inc. | Methods of treating duchenne muscular dystrophy using aav mini-dystrophin gene therapy |
US20230399656A1 (en) | 2020-11-03 | 2023-12-14 | Pfizer Inc. | Methods for Purification of AAV Vectors by Anion Exchange Chromatography |
WO2022137076A1 (en) | 2020-12-23 | 2022-06-30 | Pfizer Inc. | Methods for purification of aav vectors by affinity chromatography |
US11760788B2 (en) | 2021-03-02 | 2023-09-19 | Pathways Neuro Pharma, Inc. | Neuroreceptor compositions and methods of use |
CN117377500A (zh) | 2021-04-26 | 2024-01-09 | 阿雷克森国际制药有限公司 | 具有改善的组织向性的腺相关病毒载体衣壳 |
CN113308492B (zh) * | 2021-05-31 | 2023-03-21 | 北京中因科技有限公司 | 一种用于复制性aav的检测方法 |
WO2023114816A1 (en) | 2021-12-14 | 2023-06-22 | Neurogene, Inc. | Recombinant optimized galc constructs and methods for treating galc-associated disorders |
GB202201242D0 (en) | 2022-01-31 | 2022-03-16 | Univ Edinburgh | Recombinant optimized mecp2 cassettes and methods for treating rett syndrome and related disorders |
GB202206336D0 (en) | 2022-04-29 | 2022-06-15 | Univ Edinburgh | Recombinant therapeutic FMR1 constructs and methods of treating fragile X syndrome and related disorders |
WO2024003687A1 (en) | 2022-06-28 | 2024-01-04 | Pfizer Inc. | Nucleic acids encoding acid alpha-glucosidase (gaa) and vectors for gene therapy |
WO2024038365A1 (en) | 2022-08-16 | 2024-02-22 | Pfizer Inc. | Methods for purification of aav vectors by anion exchange chromatography |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5436146A (en) * | 1989-09-07 | 1995-07-25 | The Trustees Of Princeton University | Helper-free stocks of recombinant adeno-associated virus vectors |
US5173414A (en) * | 1990-10-30 | 1992-12-22 | Applied Immune Sciences, Inc. | Production of recombinant adeno-associated virus vectors |
EP0689601B1 (de) * | 1993-02-22 | 2006-10-04 | The Rockefeller University | Herstellung von helfer-freien retroviren mit hohem titer mittels transienter transfektion |
-
1996
- 1996-06-06 AU AU60985/96A patent/AU707862B2/en not_active Expired
- 1996-06-06 WO PCT/US1996/009405 patent/WO1996040240A1/en active IP Right Grant
- 1996-06-06 JP JP50173097A patent/JP4108746B2/ja not_active Expired - Lifetime
- 1996-06-06 CA CA2221293A patent/CA2221293C/en not_active Expired - Lifetime
- 1996-06-06 DE DE69627532T patent/DE69627532T2/de not_active Expired - Lifetime
- 1996-06-06 EP EP96918295A patent/EP0871483B1/de not_active Expired - Lifetime
- 1996-06-06 AT AT96918295T patent/ATE237352T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP0871483A4 (de) | 2000-01-05 |
DE69627532D1 (de) | 2003-05-22 |
AU707862B2 (en) | 1999-07-22 |
EP0871483A1 (de) | 1998-10-21 |
WO1996040240A1 (en) | 1996-12-19 |
CA2221293A1 (en) | 1996-12-19 |
CA2221293C (en) | 2012-09-18 |
AU6098596A (en) | 1996-12-30 |
JP4108746B2 (ja) | 2008-06-25 |
JPH11510371A (ja) | 1999-09-14 |
EP0871483B1 (de) | 2003-04-16 |
DE69627532T2 (de) | 2004-01-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE237352T1 (de) | Helfervirus-freie herstellung von aav | |
ATE91714T1 (de) | Transformation von trichoderma. | |
ATE328068T1 (de) | Papillomavirus vakzine | |
GR880100248A (el) | Ουσιες για την προφυλαξη και θεραπεια ρετροιογενην φλεγμωνων. | |
DE69332626D1 (de) | Herstellung von Brillenlinsen | |
DE3678151D1 (de) | Dispersionen von fasern in kautschuk. | |
ES2035920T3 (es) | Aerolizacion de agente proteico terapeutico. | |
FI951138A (fi) | Puuttellisia adenovirusvektoreita ja niiden käyttö geeniterapiassa | |
FI904827A0 (fi) | Kalciumkanalsammansaettningar och -foerfaranden. | |
DE3788984D1 (de) | Verwendung von Prostaglandin-D2-aktiven-Substanzen zur Behandlung von Augen-Hypertension und von Glaukom. | |
DE69001551D1 (de) | Latexfilme von naturkautschuk von modifizierter zugfestigkeit. | |
ATE112317T1 (de) | Bcrf1-proteine als inhibitoren von interferon- g(g). | |
DE69434777D1 (de) | Verwendung von Relaxin zur Herstellung von therapeutischen Mitteln | |
ATE45675T1 (de) | Verwendung von interferon gamma (ifn-gamma) zur herstellung von praeparationen zur behandlung rheumatischer erkrankungen. | |
PT870015E (pt) | Novas xilanases os genes que as codificam e as suas utilizacoes | |
HUP0001184A2 (hu) | "Basic Helix-Loop-Helix" (bHLH) családba tartozó polipeptidek és a megfelelő nukleinsav-szekvenciák | |
ATE149344T1 (de) | Verwendung von hydroxynaphthalinen in färbemitteln | |
DE3689175D1 (de) | Rekombinante Wirte, deren Herstellung und Verwendung. | |
SU888095A1 (ru) | Регулируемый источник посто нного тока | |
DE69400223D1 (de) | Verwendung von Textilbehandlungszusammensetzungen | |
DE3881953D1 (de) | Expression von fremden genen in drosophila-zellen. | |
DE59003265D1 (de) | Verwendung von nor-verapamil und nor-gallopamil als antiarteriosklerotica. | |
DE69011565D1 (de) | Herstellung von Pre-Kaseinaten und Verwendung. | |
DE69307083D1 (de) | Verbesserungen in/oder bezüglich der Herstellung von ophtalmischen Linsen | |
ES2070210T3 (es) | Derivado del activador de plasminogeno de tejido. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |