ATE214100T1 - Herstellung von rekombinanten proteinen in einem rinderadenovirus-expressionsvektorsystem - Google Patents

Herstellung von rekombinanten proteinen in einem rinderadenovirus-expressionsvektorsystem

Info

Publication number
ATE214100T1
ATE214100T1 AT95902725T AT95902725T ATE214100T1 AT E214100 T1 ATE214100 T1 AT E214100T1 AT 95902725 T AT95902725 T AT 95902725T AT 95902725 T AT95902725 T AT 95902725T AT E214100 T1 ATE214100 T1 AT E214100T1
Authority
AT
Austria
Prior art keywords
bovine adenovirus
production
expression vector
recombinant proteins
vector system
Prior art date
Application number
AT95902725T
Other languages
English (en)
Inventor
Suresh K Mittal
Frank L Graham
Ludvik Prevec
Lorne A Babiuk
Original Assignee
Univ Saskatchewan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Saskatchewan filed Critical Univ Saskatchewan
Application granted granted Critical
Publication of ATE214100T1 publication Critical patent/ATE214100T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/10011Adenoviridae
    • C12N2710/10311Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/10011Adenoviridae
    • C12N2710/10311Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/10011Adenoviridae
    • C12N2710/10311Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351Methods of production or purification of viral material
    • C12N2710/10352Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
AT95902725T 1993-12-09 1994-12-09 Herstellung von rekombinanten proteinen in einem rinderadenovirus-expressionsvektorsystem ATE214100T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/164,292 US5820868A (en) 1993-12-09 1993-12-09 Recombinant protein production in bovine adenovirus expression vector system
PCT/CA1994/000678 WO1995016048A2 (en) 1993-12-09 1994-12-09 Recombinant protein production in bovine adenovirus expression vector system

Publications (1)

Publication Number Publication Date
ATE214100T1 true ATE214100T1 (de) 2002-03-15

Family

ID=22593842

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95902725T ATE214100T1 (de) 1993-12-09 1994-12-09 Herstellung von rekombinanten proteinen in einem rinderadenovirus-expressionsvektorsystem

Country Status (6)

Country Link
US (5) US5820868A (de)
EP (1) EP0736100B1 (de)
AT (1) ATE214100T1 (de)
AU (1) AU1189195A (de)
DE (1) DE69430071T2 (de)
WO (1) WO1995016048A2 (de)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5820868A (en) 1993-12-09 1998-10-13 Veterinary Infectious Disease Organization Recombinant protein production in bovine adenovirus expression vector system
US6019978A (en) 1995-06-05 2000-02-01 The Wistar Institute Of Anatomy And Biology Replication-defective adenovirus human type 5 recombinant as a vaccine carrier
AUPN477695A0 (en) * 1995-08-14 1995-09-07 Commonwealth Scientific And Industrial Research Organisation Gene therapy
US20020193580A1 (en) * 1995-12-15 2002-12-19 Mitchell Lloyd G. Methods and compositions for use in spliceosome mediated RNA trans-splicing
US20060088938A1 (en) * 1995-12-15 2006-04-27 Mitchell Lloyd G Methods and compositions for use in spliceosome mediated RNA trans-splicing in plants
US20030027250A1 (en) * 1995-12-15 2003-02-06 Mitchell Lloyd G. Methods and compositions for use in spliceosome mediated RNA trans-splicing
FR2748753B1 (fr) * 1996-05-20 1998-08-14 Transgene Sa Nouveaux vecteurs adenoviraux pour la therapie genique
EP0979101B1 (de) * 1996-07-03 2010-10-27 Merial, Inc. Rekombinanter hunde-adenovirus 2 (cav2), welcher exogene dna enthält
AU4255397A (en) * 1996-09-06 1998-03-26 Trustees Of The University Of Pennsylvania, The Chimpanzee adenovirus vectors
US6420629B1 (en) * 1996-09-09 2002-07-16 B.C. Research Inc. Process of increasing plant growth and yield and modifying cellulose production in plants
FR2761689B1 (fr) 1997-04-02 1999-06-25 Transgene Sa Fibre adenovirale modifiee et adenovirus cibles
JP4812914B2 (ja) * 1997-06-23 2011-11-09 ユニバーシティ オブ サスカチュワン ウシアデノウイルスタイプ3ゲノム
US20030045492A1 (en) * 1997-08-13 2003-03-06 Tang De-Chu C. Vaccination by topical application of recombinant vectors
US20030125278A1 (en) * 1997-08-13 2003-07-03 Tang De-Chu C. Immunization of animals by topical applications of a salmonella-based vector
US6706693B1 (en) 1997-08-13 2004-03-16 The Uab Research Foundation Vaccination by topical application of genetic vectors
US6348450B1 (en) * 1997-08-13 2002-02-19 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom and uses thereof
US6716823B1 (en) 1997-08-13 2004-04-06 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom, and uses thereof
US7135191B2 (en) * 1997-09-04 2006-11-14 Zsolt Istvan Hertelendy Urogenital or anorectal transmucosal vaccine delivery system
CA2349760C (en) * 1998-11-02 2012-02-21 University Of Saskatchewan Bovine cells expressing adeno virus essential functions for propagation of recombinant adenoviral vectors
US6303362B1 (en) * 1998-11-19 2001-10-16 The Board Of Trustees Of The Leland Stanford Junior University Adenoviral vector and methods for making and using the same
JP2002541815A (ja) * 1999-04-09 2002-12-10 シェーリング−プラウ・リミテッド ウシアデノウイルス1型から誘導された組換えアデノウイルスおよび変異アデノウイルス
US6451319B1 (en) * 1999-04-09 2002-09-17 Schering-Plough Veterinary Corporation Recombinant and mutant adenoviruses
US20040009936A1 (en) * 1999-05-03 2004-01-15 Tang De-Chu C. Vaccine and drug delivery by topical application of vectors and vector extracts
WO2001011066A1 (de) * 1999-08-06 2001-02-15 DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung Nicht-humaner adenoviraler vektor für den gentransfer, seine anwendung und seine herstellung
JP4799789B2 (ja) * 1999-08-25 2011-10-26 メルク・シャープ・エンド・ドーム・コーポレイション ヒト細胞中での発現用に最適化された合成ヒトパピローマウイルス遺伝子
AU2001263689B2 (en) * 2000-05-31 2007-03-22 University Of Saskatchewan Modified bovine adenovirus having altered tropism
US20040126774A1 (en) * 2001-01-08 2004-07-01 Mitchell Lioyd G. Correction of factor VIII genetic defects using spliceosome mediated RNA trans splicing
EP1427816A4 (de) 2001-09-18 2004-12-01 Clontech Lab Inc Auf stellenspezifischer rekombinase beruhendes verfahren zur herstellung von adenovirusvektoren
US7399753B2 (en) * 2002-02-25 2008-07-15 Virxsys Corporation Trans-splicing mediated photodynamic therapy
CA2485341A1 (en) * 2002-05-08 2004-06-17 Intronn, Inc. Use of spliceosome mediated rna trans-splicing to confer cell selective replication to adenoviruses
US7687615B2 (en) * 2002-07-19 2010-03-30 University Of Saskatchewan PAV regions for encapsidation and E1 transcriptional control
CA2503247A1 (en) * 2002-10-23 2004-05-06 Intronn, Inc. Screening methods for identification of efficient pre-trans-splicing molecules
US7264818B2 (en) * 2003-06-10 2007-09-04 University Of Saskatchewan BAV packaging regions and E1 transcriptional control regions
JP5698426B2 (ja) * 2003-06-10 2015-04-08 ユニバーシティ オブ サスカチュワン キメラアデノウイルスキャプシドタンパク質
SI1670925T1 (sl) * 2003-10-02 2013-10-30 Crucell Holland B.V. Pakirne celice za rekombinantni adenovirus
WO2005067506A2 (en) * 2004-01-06 2005-07-28 Cedars-Sinai Medical Center Method for monitoring and generating high capacity gutless adenoviral vector
US7968334B2 (en) * 2004-01-23 2011-06-28 Virxsys Corporation Expression of apoAI and variants thereof using spliceosome mediated RNA trans-splicing
US8053232B2 (en) * 2004-01-23 2011-11-08 Virxsys Corporation Correction of alpha-1-antitrypsin genetic defects using spliceosome mediated RNA trans splicing
CA2553828A1 (en) * 2004-01-23 2005-08-04 Intronn, Inc. Expression of apoa-1 and variants thereof using spliceosome mediated rna trans-splicing
AU2005249450A1 (en) 2004-05-27 2005-12-15 Centocor, Inc. Cynomolgus prostate specific antigen
AU2005329405A1 (en) * 2004-06-15 2006-09-28 Centocor, Inc. Method for screening agents against human prostate disease
US20060094110A1 (en) * 2004-07-30 2006-05-04 Mcgarrity Gerard J Use of spliceosome mediated RNA trans-splicing for immunotherapy
US20060134658A1 (en) * 2004-08-09 2006-06-22 Garcia-Blanco Mariano A Use of RNA trans-splicing for generation of interfering RNA molecules
DE102004047492B4 (de) * 2004-09-23 2006-07-20 Jost-Werke Gmbh & Co. Kg Verfahren zum Übertragen von elektrischer, pneumatischer oder hydraulischer Energie sowie ein Energieübertragungssystem
US7871795B2 (en) 2004-10-08 2011-01-18 Virxsys Corporation Targeted trans-splicing of highly abundant transcripts for in vivo production of recombinant proteins
WO2006083331A2 (en) * 2004-10-08 2006-08-10 Intronn, Inc Use of rna trans-splicing for antibody gene transfer and antibody polypeptide production
WO2006091722A2 (en) * 2005-02-23 2006-08-31 Uab Research Foundation Alkyl-glycoside enhanced vaccination
EP1877088B1 (de) * 2005-04-11 2014-12-17 THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES Impfstoff gegen pandemische stämme von grippeviren
US20150284729A1 (en) * 2012-11-15 2015-10-08 National University Of Singapore Univariant Extrinsic Initiator Control System for Microbes and an In Vitro Assembly of Large Recombinant DNA Molecules From Multiple Components
KR102543774B1 (ko) 2016-09-20 2023-06-19 베링거잉겔하임베트메디카게엠베하 신규한 프로모터
ES2956050T3 (es) 2016-09-20 2023-12-12 Boehringer Ingelheim Vetmedica Gmbh Vectores de adenovirus caninos
WO2018054822A1 (en) 2016-09-20 2018-03-29 Boehringer Ingelheim Vetmedica Gmbh New swine influenza vaccine
AR109539A1 (es) 2016-09-20 2018-12-19 Boehringer Ingelheim Vetmedica Gmbh Sitio de inserción orf70 de ehv
US10525127B2 (en) * 2018-03-31 2020-01-07 Femtomab P14.7 protein and uses thereof as vaccine adjuvant

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB185573A (en) 1921-07-12 1922-09-14 Richard Saleck Apparatus for measuring off and cutting strings in bundles
US3962424A (en) * 1974-01-31 1976-06-08 Recherche Et Industrie Therapeutiques (R.I.T.) Live brovine adenovirus vaccines, preparation thereof and method of vaccination using them
US4510245A (en) * 1982-11-18 1985-04-09 Chiron Corporation Adenovirus promoter system
ZA858044B (en) * 1984-11-01 1987-05-27 American Home Prod Oral vaccines
FR2573436B1 (fr) * 1984-11-20 1989-02-17 Pasteur Institut Adn recombinant comportant une sequence nucleotidique codant pour un polypeptide determine sous le controle d'un promoteur d'adenovirus, vecteurs contenant cet adn recombinant, cellules eucaryotes transformees par cet adn recombinant, produits d'excretion de ces cellules transformees et leurs applications, notamment a la constitution de vaccins
JPS62502662A (ja) * 1985-05-01 1987-10-15 ジエネテイツクス・インスチチユ−ト・インコ−ポレ−テツド 外来性dnaの高レベル増幅および発現
CA1319101C (en) 1986-09-03 1993-06-15 Marta Iris Sabara Rotavirus nucleocapsid protein with or without binding peptides as immunologic carriers for macromolecules
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
US5151267A (en) 1988-07-15 1992-09-29 University Of Saskatchewan Bovine herpesvirus type 1 polypeptides and vaccines
FR2642797B1 (fr) * 1989-01-10 1991-05-17 Secmair Dispositif d'asservissement de la pression d'appui au sol d'un rouleau equipant une tete de coupe, notamment de fauchage
FR2642767B1 (fr) 1989-01-19 1993-10-01 Transgene Sa Vecteurs d'expression de proteines heterologues dans les cellules eucaryotes, lignees cellulaires obtenues et procede pour leur preparation
ATE140154T1 (de) * 1989-03-24 1996-07-15 Wistar Inst Rekombinanter cytomegalovirus-impfstoff
GB9001766D0 (en) * 1990-01-25 1990-03-28 Univ Court Of The University O Vaccines
FR2657880A1 (fr) * 1990-02-02 1991-08-09 Fondation Nale Transfusion San Production de proteines recombinantes a partir de cellules lymphoblastouides humaines et d'heteromyelomes.
US5543328A (en) 1993-08-13 1996-08-06 Genetic Therapy, Inc. Adenoviruses having modified fiber proteins
US5820868A (en) 1993-12-09 1998-10-13 Veterinary Infectious Disease Organization Recombinant protein production in bovine adenovirus expression vector system
US5846782A (en) 1995-11-28 1998-12-08 Genvec, Inc. Targeting adenovirus with use of constrained peptide motifs
WO1996022398A1 (en) 1995-01-17 1996-07-25 Environmental Projects, Inc. Method for purification of saline minerals
US5770442A (en) 1995-02-21 1998-06-23 Cornell Research Foundation, Inc. Chimeric adenoviral fiber protein and methods of using same
WO1997020575A1 (en) 1995-12-08 1997-06-12 The University Of Alabama At Birmingham Research Foundation Targeted adenovirus vectors
JP4812914B2 (ja) 1997-06-23 2011-11-09 ユニバーシティ オブ サスカチュワン ウシアデノウイルスタイプ3ゲノム
US5922576A (en) 1998-02-27 1999-07-13 The John Hopkins University Simplified system for generating recombinant adenoviruses
AU2001263689B2 (en) 2000-05-31 2007-03-22 University Of Saskatchewan Modified bovine adenovirus having altered tropism
CA2415347A1 (en) 2000-07-14 2002-01-24 University Of Saskatchewan Adenovirus vectors comprising introns
US20030143200A1 (en) 2001-09-24 2003-07-31 Tikoo Suresh K. Porcine adenovirus E1 region
US7569217B2 (en) 2001-09-24 2009-08-04 University Of Saskatchewan Porcine adenovirus E1 and E4 regions

Also Published As

Publication number Publication date
WO1995016048A2 (en) 1995-06-15
US20020192185A1 (en) 2002-12-19
DE69430071T2 (de) 2002-11-28
DE69430071D1 (de) 2002-04-11
US6379944B1 (en) 2002-04-30
AU1189195A (en) 1995-06-27
US6001591A (en) 1999-12-14
US5820868A (en) 1998-10-13
EP0736100A1 (de) 1996-10-09
EP0736100B1 (de) 2002-03-06
US6086890A (en) 2000-07-11
US7025967B2 (en) 2006-04-11
WO1995016048A3 (en) 1995-09-28

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