ATE12838T1 - Verfahren zum trennen einer eiweissartigen substanz mittels affinitaetsfiltrierung einer diese substanz enthaltenden loesung und verwendung dieses verfahrens fuer enzymatische bestimmungen. - Google Patents

Verfahren zum trennen einer eiweissartigen substanz mittels affinitaetsfiltrierung einer diese substanz enthaltenden loesung und verwendung dieses verfahrens fuer enzymatische bestimmungen.

Info

Publication number
ATE12838T1
ATE12838T1 AT80400913T AT80400913T ATE12838T1 AT E12838 T1 ATE12838 T1 AT E12838T1 AT 80400913 T AT80400913 T AT 80400913T AT 80400913 T AT80400913 T AT 80400913T AT E12838 T1 ATE12838 T1 AT E12838T1
Authority
AT
Austria
Prior art keywords
substance
affinity
protein
separating
solution containing
Prior art date
Application number
AT80400913T
Other languages
English (en)
Inventor
Jean-Claude Nicolas
Beatrice Terouanne
Bernard Descomps
De Paulet Andre Crastes
Original Assignee
Inst Nat Sante Rech Med
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Nat Sante Rech Med filed Critical Inst Nat Sante Rech Med
Application granted granted Critical
Publication of ATE12838T1 publication Critical patent/ATE12838T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT80400913T 1979-06-21 1980-06-19 Verfahren zum trennen einer eiweissartigen substanz mittels affinitaetsfiltrierung einer diese substanz enthaltenden loesung und verwendung dieses verfahrens fuer enzymatische bestimmungen. ATE12838T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR7915992A FR2459479B1 (fr) 1979-06-21 1979-06-21 Procede de separation d'une substance proteinique a partir d'une solution la contenant par filtration d'affinite et application dudit procede aux dosages enzymatiques
EP80400913A EP0022005B1 (de) 1979-06-21 1980-06-19 Verfahren zum Trennen einer eiweissartigen Substanz mittels Affinitätsfiltrierung einer diese Substanz enthaltenden Lösung und Verwendung dieses Verfahrens für enzymatische Bestimmungen

Publications (1)

Publication Number Publication Date
ATE12838T1 true ATE12838T1 (de) 1985-05-15

Family

ID=9226940

Family Applications (1)

Application Number Title Priority Date Filing Date
AT80400913T ATE12838T1 (de) 1979-06-21 1980-06-19 Verfahren zum trennen einer eiweissartigen substanz mittels affinitaetsfiltrierung einer diese substanz enthaltenden loesung und verwendung dieses verfahrens fuer enzymatische bestimmungen.

Country Status (6)

Country Link
US (1) US4350760A (de)
EP (1) EP0022005B1 (de)
JP (1) JPS5637009A (de)
AT (1) ATE12838T1 (de)
DE (1) DE3070502D1 (de)
FR (1) FR2459479B1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4476093A (en) * 1980-09-30 1984-10-09 The Green Cross Corporation Kit for preparing sample for use in endotoxin test
US4414336A (en) * 1981-01-28 1983-11-08 The Green Cross Corporation Method for preparing sample for use in endotoxin test
EP0073593A1 (de) * 1981-09-01 1983-03-09 E.I. Du Pont De Nemours And Company Grössendiskriminierende heterogene Immunzusammensetzung
US4485017A (en) * 1982-12-22 1984-11-27 Cetus Corporation Isolation of human interferon by immunosorbent and high performance liquid chromatography
US4675113A (en) * 1984-11-28 1987-06-23 University Patents, Inc. Affinity chromatography using dried calcium alginate-magnetite separation media in a magnetically stabilized fluidized bed
US4721572A (en) * 1985-07-12 1988-01-26 Miles Laboratories, Inc. Purfication of blood clotting factors and other blood proteins on non-carbohydrate sulfated matrices
DK570485D0 (da) * 1985-12-10 1985-12-10 Novo Industri As Humant conglutinin
US4847194A (en) * 1987-03-13 1989-07-11 Becton, Dickinson And Company Colorimetric detection of delta-5-3-ketosteroid isomerase and immunoassay based thereon
US4788136A (en) * 1987-04-07 1988-11-29 Abbott Laboratories Diagnostic immunoassay by solid phase separation for digoxin
US5003054A (en) * 1987-04-07 1991-03-26 Abbott Laboratories Ouabain triacetate derivative compounds
US4973554A (en) * 1987-05-29 1990-11-27 National Research Council Of Canada Affinity process for trypsin purification and stabilization
IL93205A0 (en) * 1989-01-30 1990-11-05 Us Agriculture Column for the separation of macromolecules
US5085779A (en) * 1989-06-07 1992-02-04 J. T. Baker, Inc. Polyethyleneimine matrixes for affinity chromatography
US5092992A (en) * 1989-06-07 1992-03-03 J. T. Baker Inc. Polyethyleneimine matrixes for affinity chromatography
CA2022518A1 (en) * 1989-08-04 1991-02-05 Richard P. Watts Heterogeneous binding assays
US5252492A (en) * 1991-03-12 1993-10-12 University Of Utah Research Foundation Fluorescence assay for ligand or receptor utilizing size exclusion
DE9216110U1 (de) * 1992-11-26 1993-01-28 Biolab GmbH, 80995 München Progesteron-Schnelltest für Mensch und Haustiere
WO1994029722A1 (en) * 1993-06-08 1994-12-22 Chronomed, Inc. Two-phase optical assay method and apparatus
DE4407142A1 (de) * 1994-03-04 1995-09-07 Thomae Gmbh Dr K Heterogener Immunassay und dessen Verwendung zur Bestimmung von Proteinen
NL1006680C2 (nl) * 1997-03-04 1998-09-07 Alexander Adrianus Moen Werkwijze voor het detecteren van de aanwezigheid van een immunologisch reactief molecuul in een monster.

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3502545A (en) * 1968-09-27 1970-03-24 Monsanto Co Process for the separation of water-soluble polymeric materials from unbound protein or peptide using semiporous gel
DE2061009A1 (de) * 1969-12-18 1971-06-24 Exploaterings Ab Tbf Verfahren zur Fixierung von Polymeren
CS162953B1 (de) * 1972-05-10 1975-07-31
NL171930C (nl) * 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
DE2319495C2 (de) * 1973-04-17 1985-01-10 Yeda Research And Development Co., Ltd., Rehovot Verfahren zum selektiven, reversiblen Binden von Biomolekülen an ein Adsorbens in einer chromatographischen Säule
US4138474A (en) * 1973-05-01 1979-02-06 Wisconsin Alumni Research Foundation Method and device for immunoassay
DE2418978A1 (de) * 1974-04-19 1975-10-30 Boehringer Mannheim Gmbh Steroid-delta-isomerase
US4125492A (en) * 1974-05-31 1978-11-14 Pedro Cuatrecasas Affinity chromatography of vibrio cholerae enterotoxin-ganglioside polysaccharide and the biological effects of ganglioside-containing soluble polymers
NZ183970A (en) * 1976-05-03 1979-06-08 Beckman Instruments Inc Composite for use in immunoassay; derivatized polysaccharide matrix covalently coupled to an antibody by a bifunctional coupling agent; immunoassay procedure
US4224439A (en) * 1977-02-08 1980-09-23 Development Finance Corporation Of New Zealand Activated matrix and method of activation
US4235960A (en) * 1977-07-29 1980-11-25 The Medical College Of Wisconsin, Inc. Competitive enzyme-linked immunoassay
DE2758507C3 (de) * 1977-12-28 1981-05-27 Byk-Mallinckrodt Chemische Produkte Gmbh, 6057 Dietzenbach Stabilisierte biochemische Präparate, Verfahren zu ihrer Herstellung und ihre Verwendung
US4205130A (en) * 1979-03-12 1980-05-27 Pirkko Vihko Method of purification of human prostatic acid phosphatase

Also Published As

Publication number Publication date
FR2459479B1 (fr) 1983-07-08
EP0022005B1 (de) 1985-04-17
JPS5637009A (en) 1981-04-10
EP0022005A1 (de) 1981-01-07
DE3070502D1 (en) 1985-05-23
FR2459479A1 (fr) 1981-01-09
US4350760A (en) 1982-09-21

Similar Documents

Publication Publication Date Title
ATE12838T1 (de) Verfahren zum trennen einer eiweissartigen substanz mittels affinitaetsfiltrierung einer diese substanz enthaltenden loesung und verwendung dieses verfahrens fuer enzymatische bestimmungen.
ES2027325T3 (es) Ultrafiltracion de afinidad con flujo tangencial.
ES2097747T3 (es) Molecula de adhesion intercelular 2 y sus ligandos de union.
PT87390A (pt) Intercellular adhesion molecules and their binding ligands
DE69020143D1 (de) Verfahren zur verwendung und herstellung von peptiden.
DE3773859D1 (de) Verfahren zur isomerisierung eines kohlenwasserstoffeinsatzstromes.
NL190308B (nl) Werkwijze ter afscheiding van eiwitten uit melkwei, alsmede toepassing van deze eiwitten.
DE60231651D1 (de) Proteinreinigungsverfahren
ES2071706T3 (es) Metodo para fraccionar proteinas plasmaticas.
Caspers et al. Separator isoelectric focusing: an improved method of protein analysis and purification
DE69028513D1 (de) Träger zur bindung von antiphospholipid-antikörpern, diese antikörper verwendender immunotest und kit dafür
DE3865963D1 (de) Elektrophoretisches gel hoher aufloesung sowie methode zur abscheidung von serumproteinen.
ATE299889T1 (de) Neues verfahren zur trennung von proteinen unter verwendung eines ca++ enthaltenden elutionsmittels
GB1106818A (en) Mixture of carrier ampholytes and method of preparation thereof
DE69327059T2 (de) Verfahren zur herstellung von testelementen
ATE32436T1 (de) Verfahren zur fraktionierten auftrennung von proteingemischen mittels membranen.
ATE191568T1 (de) Spezifische bindungssubstanzen für antikörper und deren verwendung für immunoassays oder vakzine
DE3485711D1 (de) Immunoadsorbens und verfahren zur zurueckgewinnung von vitamin-k-abhaengigen proteinen mittels dieses adsorbens.
DE3750397D1 (de) Verfahren zum testen von körperflüssigkeiten.
DE3762577D1 (de) Verfahren und vorrichtung zum abtrennen von an schuettgut gebundenen fremdstoffen.
DE3482467D1 (de) Verfahren zur oligomerisierung von alpha-olefinen.
DE3766521D1 (de) Verfahren zum verleihen von verbesserten statischen reibungseigenschaften an kohlenstoff enthaltenden bremsmaterialien.
Lester et al. Heterogeneity of human α-fetoprotein as revealed by isoelectric focusing in urea-containing gels
ES2080804T3 (es) Separacion de anticuerpos anti-metal quelato.
DE3785852D1 (de) Verfahren zur chromatographischen trennung von metallionen.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties