AT75154B - Process for the preparation of new hormones such as ovaries, corpus luteum and placenta and their aqueous solutions. - Google Patents
Process for the preparation of new hormones such as ovaries, corpus luteum and placenta and their aqueous solutions.Info
- Publication number
- AT75154B AT75154B AT75154DA AT75154B AT 75154 B AT75154 B AT 75154B AT 75154D A AT75154D A AT 75154DA AT 75154 B AT75154 B AT 75154B
- Authority
- AT
- Austria
- Prior art keywords
- hormones
- preparation
- ovaries
- placenta
- aqueous solutions
- Prior art date
Links
- 229940088597 hormone Drugs 0.000 title claims description 15
- 239000005556 hormone Substances 0.000 title claims description 15
- 238000000034 method Methods 0.000 title claims description 8
- 239000007864 aqueous solution Substances 0.000 title claims description 5
- 210000001672 ovary Anatomy 0.000 title claims description 5
- 238000002360 preparation method Methods 0.000 title claims description 5
- 210000004246 corpus luteum Anatomy 0.000 title claims description 4
- 210000002826 placenta Anatomy 0.000 title claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 239000012452 mother liquor Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000004821 distillation Methods 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 claims 2
- 238000007792 addition Methods 0.000 claims 1
- 238000004508 fractional distillation Methods 0.000 claims 1
- 238000001556 precipitation Methods 0.000 claims 1
- 239000007858 starting material Substances 0.000 claims 1
- 230000001225 therapeutic effect Effects 0.000 claims 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 8
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 8
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 235000012000 cholesterol Nutrition 0.000 description 4
- 150000001840 cholesterol esters Chemical class 0.000 description 3
- 239000003208 petroleum Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
<Desc/Clms Page number 1>
Verfahren zur Darstellung von neuen Hormonen aus Ovarien, Corpus luteum oder Placenta und ihren wässerigen Lösungen.
EMI1.1
aus Ovarien, Corpus luteum oder Placcnta dadurch gewonnen, dass das Gewebe im Vakuum getrocknet oder mit Salzen oder flüchtigen Lösungsmitteln entwässert wird, worauf man es mit wasserlösenden flüchtigen Lösungsmitteln, wie Aceton oder Alkohol, extrahiert, den Auszug 'inengt und mit einem mit Wasser nicht mischbaren Lösungsmittel, wie Petroläther oder Äther,
EMI1.2
Lösungsmitteln entwässerte Gewebe unmittelbar mit einem Wasser nicht lösenden Lösung- mitTel, wie beispielsweise Ather oder Petroläther ausgewogen wird,
worauf dann nach dem Ver- dampfen des Lösungsmittels die Phosphatide mit Aceton gefällt und die Hormone aus der : ectonigen Mutterlauge gewonnen werden. Als einen dritten Weg zur Gewinnung der Hormone beschreibt das Zusatzpatent Nr. 75153 die unmittelbare Extrazktion des Gewebes mit schwach angesäuertem Wasser.
In weiterer Ausbildung des Verfahrens des Stamm- und ersten Zusatzpatentes wurde mm gefunden, dass die Hormone aus der Mutterlauge in einfacherer Weise und in besserer
EMI1.3
190 bis 210 ein Gemenge der Hormone mit Cholesterin und Cholesterinestern über. Durch Behandlung dieses Destillates mit Alkohol Werden die Hormone in Lösung gebracht, während einzelne Cholesterinester und Cholesterin sich kristallinisch ausscheiden. Zur Darstellung der gesuchten Substanz in chemisch reinem Zustande kann alsdann nach dem Verdampfen des Alkohols eine zweite Destillation (Refraktioniren) in hohem Vakuum vorgenommen Werden.
EMI1.4
des Bades von 2400 und bei einer Innentemperatur von 1930 C (unkorrigiert) über.
Bei gewöhn- licher Temperatur ist sie ein helles dickfttissigcs Öl und zeigt typische Cholesterinreaktionen.
Sie enthält nur die Elemente Kohlenstoff, Wasserstoff und Sauerstoff. Bei der Elementar-
EMI1.5
<Desc/Clms Page number 2>
Um 0 die Trennung der Hormone von den übrigen Bestandteilen des Rückstandes der amtonigen Mutterlauge zu erleichtern, kann es unter Umständen zweckmässig sein t der ersten Destillation eine Behandlung mit Äthyl-oder Methylalkohol vorauszuschicken, durch welche bereits ein grosser Anteil der in kaltem Alkohol oder Methylakohol weniger löslichen Cholesterin- ester und des Cholesterins ausgeschieden wird.
<Desc / Clms Page number 1>
Process for the preparation of new hormones from ovaries, corpus luteum or placenta and their aqueous solutions.
EMI1.1
Obtained from ovaries, corpus luteum or placcnta by drying the tissue in a vacuum or dehydrating it with salts or volatile solvents, whereupon it is extracted with water-dissolving volatile solvents such as acetone or alcohol, the extract is concentrated and one with water not miscible solvents, such as petroleum ether or ether,
EMI1.2
Tissues dehydrated with solvents are immediately weighed out with a water-insoluble solution such as ether or petroleum ether,
whereupon, after the solvent has evaporated, the phosphatides are precipitated with acetone and the hormones are obtained from the ectonic mother liquor. As a third way of obtaining the hormones, the additional patent No. 75153 describes the immediate extraction of the tissue with weakly acidified water.
In further development of the process of the parent and first additional patent, it was found that the hormones from the mother liquor in a simpler way and in a better way
EMI1.3
190 to 210 a mixture of the hormones with cholesterol and cholesterol esters about. By treating this distillate with alcohol, the hormones are dissolved, while individual cholesterol esters and cholesterol are precipitated in crystalline form. Then, after the alcohol has evaporated, a second distillation (refraction) can be carried out in a high vacuum to produce the substance sought in a chemically pure state.
EMI1.4
of the bath of 2400 and an internal temperature of 1930 C (uncorrected) above.
At ordinary temperature it is a light, thick oil and shows typical cholesterol reactions.
It only contains the elements carbon, hydrogen and oxygen. In the elementary
EMI1.5
<Desc / Clms Page number 2>
In order to facilitate the separation of the hormones from the other constituents of the residue of the amtonous mother liquor, it may be advisable, under certain circumstances, to precede the first distillation with a treatment with ethyl or methyl alcohol, which already reduces a large proportion of the cold alcohol or methyl alcohol soluble cholesterol ester and cholesterol is excreted.
Claims (1)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT71382T | 1913-08-04 | ||
| AT75154T | 1914-07-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AT75154B true AT75154B (en) | 1919-01-10 |
Family
ID=25603599
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT75154D AT75154B (en) | 1913-08-04 | 1914-07-07 | Process for the preparation of new hormones such as ovaries, corpus luteum and placenta and their aqueous solutions. |
Country Status (1)
| Country | Link |
|---|---|
| AT (1) | AT75154B (en) |
-
1914
- 1914-07-07 AT AT75154D patent/AT75154B/en active
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