DE733809C - Process for the extraction of glycosides from Strophanthus Kombe - Google Patents

Description

Process for the production of glycosides from Strophanthus Kombe Die Extraction of the seeds from Stropha.nthus bomb is usually done by means of organic Solvent. If you use water as the extractant, some of the remains Strophanthin glycosides because of their poor solubility or their binding to accompanying substances inextractable.

It has now been found that the yield of the strophanthin glycosides in the case of aqueous extraction can be increased significantly when in the presence of Heavy metal salts such as lead acetate, copper sulfate or mercury chloride are made what is presumably due to an influence on the hydrolysis and a precipitating effect of these salts is due to the accompanying substances'.

In practice, one works in such a way that either the finely chopped ones are used Seeds intimately mixed with the solid salts and then extracted with water, or that the seeds are treated with dilute aqueous solutions of these salts.

The glycosides obtained after conventional work-up are distinguished from those obtained by the previous processes by a better effectiveness; Thus, in rats, systolic cardiac arrest is brought about by a dose of 0.05 g per kg of body weight, while the same result is only achieved with the previous products by 0.1 ig per kg (subcutaneous injection).

Examples i. zoo g seeds of Strophanthus bomb are added ground a mixture of i o g neutral and iog basic lead acetate. Slides ground mixture is made with degreased dry ligroin, then with Stirring 30o ccm of water at room temperature for several hours: Then filter. The filter residue is mixed with ioog fine-grain sand and placed in the hydraulic Press dry pressed. The press cake is' with i% i litmus-neutral lead acetate solution digested again. It is filtered and pressed dry again. All filtering and Press fluids are combined and used to remove impurities with ether shaken.

The subsequent defleading of the aqueous liquid can be carried out in the usual way Way done by hydrogen sulfide or by adding the appropriate Amount of ammonium sulfate solution. After removal of the lead sulfide or the lead sulfate from the aqueous solution of strophanthin by adding solid ammonium sulfate to precipitated to saturation. The separation of the strophanthin from the aqueous solution Instead of ammonium sulphate, it can also be mixed with an aqueous tannin solution take place. The decomposition of the precipitated strophantine-tannin compound takes place through Lead or zinc oxide in the usual way. Eventually the strophanthin turns into alcohol dissolved and precipitated from this solution with ether.

One receives 1 g of a pure white strophant lime preparation. Its melting point lies between 18o and i 9o '. Its optical rotation HD is @ - 2z to z3 '; be Methoxyl content 3.5%.

200g seeds of Strophanthus Kombe are mixed with an i o / qigen solution of mercury acetate or mercury chloride extracted several times in the cold. The mercury is removed from the combined extracts with hydrogen sulfide or another mercury-precipitating agent, with the addition of carbonate neutral Response is sustained.

The liquid separated from the mercury precipitate becomes, as in Example i described, worked up.

Claims (1)

PATENT CLAIM: Process for the production of glycosides from Strophanthus Combe by drawing out with water, characterized in that the comminuted Seeds extracted with water in the presence of heavy metal salts.