AT513859B1 - Mikro-Fluoreszenzdetektionsvorrichtung sowie Verfahren zur Detektion - Google Patents
Mikro-Fluoreszenzdetektionsvorrichtung sowie Verfahren zur Detektion Download PDFInfo
- Publication number
- AT513859B1 AT513859B1 AT2992013A AT2992013A AT513859B1 AT 513859 B1 AT513859 B1 AT 513859B1 AT 2992013 A AT2992013 A AT 2992013A AT 2992013 A AT2992013 A AT 2992013A AT 513859 B1 AT513859 B1 AT 513859B1
- Authority
- AT
- Austria
- Prior art keywords
- prism
- excited
- analyzed
- micro
- light
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 33
- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 81
- 230000005284 excitation Effects 0.000 claims abstract description 58
- 239000000523 sample Substances 0.000 claims description 15
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 8
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 8
- 239000004033 plastic Substances 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 6
- 229920000089 Cyclic olefin copolymer Polymers 0.000 claims description 5
- -1 Polyethylene terephthalate Polymers 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 239000004743 Polypropylene Substances 0.000 claims description 4
- 239000004793 Polystyrene Substances 0.000 claims description 4
- 239000004417 polycarbonate Substances 0.000 claims description 4
- 229920000515 polycarbonate Polymers 0.000 claims description 4
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 4
- 239000005020 polyethylene terephthalate Substances 0.000 claims description 4
- 229920001155 polypropylene Polymers 0.000 claims description 4
- 229920001577 copolymer Polymers 0.000 claims description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 claims description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 2
- 229910052782 aluminium Inorganic materials 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims description 2
- 238000005192 partition Methods 0.000 claims description 2
- 229920000058 polyacrylate Polymers 0.000 claims description 2
- 239000002861 polymer material Substances 0.000 claims description 2
- 229920002635 polyurethane Polymers 0.000 claims description 2
- 239000004814 polyurethane Substances 0.000 claims description 2
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 claims 1
- 238000005259 measurement Methods 0.000 description 16
- 238000011161 development Methods 0.000 description 12
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
- 108010090804 Streptavidin Proteins 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000000018 DNA microarray Methods 0.000 description 4
- 229960002685 biotin Drugs 0.000 description 4
- 235000020958 biotin Nutrition 0.000 description 4
- 239000011616 biotin Substances 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 230000010287 polarization Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 238000005102 attenuated total reflection Methods 0.000 description 2
- 239000000090 biomarker Substances 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 150000001925 cycloalkenes Chemical class 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 238000002032 lab-on-a-chip Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004452 microanalysis Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- 238000012935 Averaging Methods 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000011358 absorbing material Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 102000043871 biotin binding protein Human genes 0.000 description 1
- 108700021042 biotin binding protein Proteins 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004049 embossing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000007731 hot pressing Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 108010087904 neutravidin Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229920002397 thermoplastic olefin Polymers 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT2992013A AT513859B1 (de) | 2013-04-12 | 2013-04-12 | Mikro-Fluoreszenzdetektionsvorrichtung sowie Verfahren zur Detektion |
PCT/AT2014/000066 WO2014165879A1 (fr) | 2013-04-12 | 2014-03-31 | Micro-dispositif de détection de fluorescence ainsi que procédé associé |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT2992013A AT513859B1 (de) | 2013-04-12 | 2013-04-12 | Mikro-Fluoreszenzdetektionsvorrichtung sowie Verfahren zur Detektion |
Publications (2)
Publication Number | Publication Date |
---|---|
AT513859A4 AT513859A4 (de) | 2014-08-15 |
AT513859B1 true AT513859B1 (de) | 2014-08-15 |
Family
ID=50721502
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT2992013A AT513859B1 (de) | 2013-04-12 | 2013-04-12 | Mikro-Fluoreszenzdetektionsvorrichtung sowie Verfahren zur Detektion |
Country Status (2)
Country | Link |
---|---|
AT (1) | AT513859B1 (fr) |
WO (1) | WO2014165879A1 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102017115661A1 (de) * | 2017-07-12 | 2019-01-17 | Endress+Hauser Conducta Gmbh+Co. Kg | Optischer Sensor |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106680258A (zh) * | 2017-02-09 | 2017-05-17 | 重庆科技学院 | 一种全内反射检测工作平台 |
CN108414480B (zh) * | 2018-01-26 | 2023-03-24 | 中国海洋石油集团有限公司 | 一种原油荧光测量装置和方法 |
CN109520939B (zh) * | 2019-01-03 | 2024-05-24 | 华域视觉科技(上海)有限公司 | 一种材料检测装置 |
DE102020201112A1 (de) | 2020-01-30 | 2021-08-05 | Robert Bosch Gesellschaft mit beschränkter Haftung | Optische Auslesung von Prozessen innerhalb einer mikrofluidischen Vorrichtung |
CN113109314A (zh) * | 2021-05-28 | 2021-07-13 | 上海睿钰生物科技有限公司 | 多荧光信号检测系统和方法 |
CN115097617B (zh) * | 2022-07-15 | 2022-12-30 | 中国科学技术大学 | 一种基于平面芯片的全内反射荧光显微镜系统 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH1078390A (ja) * | 1996-09-04 | 1998-03-24 | Fuji Photo Film Co Ltd | 表面プラズモンセンサー |
DE69531125T2 (de) * | 1994-04-15 | 2004-04-08 | Agilent Technologies Inc., A Delaware Corp., Palo Alto | Nachweis einer Zielsubstanz in einer Probe |
JP2010243267A (ja) * | 2009-04-03 | 2010-10-28 | Hitachi High-Technologies Corp | ナノ構造体デバイスを利用した計測装置及び計測方法 |
WO2011019713A1 (fr) * | 2009-08-11 | 2011-02-17 | Wedge Technologies | Microscope à fond ultrasombre |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5633724A (en) * | 1995-08-29 | 1997-05-27 | Hewlett-Packard Company | Evanescent scanning of biochemical array |
ES2219962T3 (es) * | 1999-08-24 | 2004-12-01 | Leuze Electronic Gmbh + Co Kg | Dispositivo para la realizacion de inmunoensayos. |
WO2008072156A2 (fr) * | 2006-12-12 | 2008-06-19 | Koninklijke Philips Electronics N. V. | Capteur microélectronique pour détecter des particules de marquage |
KR101067348B1 (ko) | 2009-03-04 | 2011-09-23 | 한국과학기술원 | 편광각 투과 유도 프리즘 및 이를 이용한 신호 대 잡음비 향상을 위한 형광검출장치 |
-
2013
- 2013-04-12 AT AT2992013A patent/AT513859B1/de not_active IP Right Cessation
-
2014
- 2014-03-31 WO PCT/AT2014/000066 patent/WO2014165879A1/fr active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69531125T2 (de) * | 1994-04-15 | 2004-04-08 | Agilent Technologies Inc., A Delaware Corp., Palo Alto | Nachweis einer Zielsubstanz in einer Probe |
JPH1078390A (ja) * | 1996-09-04 | 1998-03-24 | Fuji Photo Film Co Ltd | 表面プラズモンセンサー |
JP2010243267A (ja) * | 2009-04-03 | 2010-10-28 | Hitachi High-Technologies Corp | ナノ構造体デバイスを利用した計測装置及び計測方法 |
WO2011019713A1 (fr) * | 2009-08-11 | 2011-02-17 | Wedge Technologies | Microscope à fond ultrasombre |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102017115661A1 (de) * | 2017-07-12 | 2019-01-17 | Endress+Hauser Conducta Gmbh+Co. Kg | Optischer Sensor |
US10359365B2 (en) | 2017-07-12 | 2019-07-23 | Endress+Hauser Conducta Gmbh+Co. Kg | Optical sensor |
Also Published As
Publication number | Publication date |
---|---|
WO2014165879A1 (fr) | 2014-10-16 |
AT513859A4 (de) | 2014-08-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE102007033124B4 (de) | Vorrichtung zur optischen Detektion von Substanzen in einem flüssigen oder gasförmigen Medium | |
AT513859B1 (de) | Mikro-Fluoreszenzdetektionsvorrichtung sowie Verfahren zur Detektion | |
EP0988526B1 (fr) | Dispositif de detection de substances chimiques et biochimiques par excitation de lumiere fluorescente et son procede de production | |
EP0938660B1 (fr) | Cellule micromecanique de mesure de transmission | |
DE69926230T2 (de) | Optische sensorvorrichtung mit evaneszenter felddetektion | |
EP1963821B1 (fr) | Puce de mesure | |
EP1237654B9 (fr) | Ensemble de recipients pour echantillons et son utilisation dans la determination de plusieurs analytes | |
DE10008006C2 (de) | SPR-Sensor und SPR-Sensoranordnung | |
EP1327135B1 (fr) | Systeme et procedes pour determiner plusieurs analytes | |
EP2522988B1 (fr) | Dispositif d'analyse optique, procédé d'analyse optique et programme informatique d'analyse optique | |
DE202010018623U1 (de) | Strukturen zur Steuerung der Lichtwechselwirkung mit mikrofluidischen Vorrichtungen | |
EP0910792A1 (fr) | Procede et dispositif pour mettre en oeuvre des tests quantitatifs d'affinite a fluorescence | |
WO1997040366A1 (fr) | Procede et dispositif permettant de mettre en evidence des reactions et des interactions d'ordre physique, chimique, biologique ou biochimique | |
WO2008132247A2 (fr) | Récipient et procédé de détection de la fluorescence | |
EP1347284B1 (fr) | Porte-échantillon avec optique intégrée | |
WO2008135566A2 (fr) | Unité de mesure et procédé d'examen optique d'un liquide pour déterminer une concentration d'un analyte | |
EP1079226B1 (fr) | Dispositif de dosage immunologique | |
DE60301821T2 (de) | Küvette für ein Lesegerät zur Bestimmung von Substanzen mittels der Evaneszenzfeldmethode | |
DE19647644C2 (de) | Mikromechanische Transmissionsmeßzelle | |
DE10324973B4 (de) | Anordnung und Verfahren zur optischen Detektion von in Proben enthaltenen chemischen, biochemischen Molekülen und/oder Partikeln | |
EP2686664B1 (fr) | Détection de milieux optiques plans en au moins deux couches à séparation optique | |
DE102009025073A1 (de) | Optischer Sensor | |
DE102004034486B4 (de) | Verfahren zum Nachweis von Lumineszenzlicht aus einer porösen Trägerstruktur | |
DE102018133037B4 (de) | Anordnung und Verfahren zur Erfassung von optischen Eigenschaften einer Probe, insbesondere zum selektiven Nachweis von biologischen Molekülen und zum Auslesen einer Molekülbelegung | |
DE10221115A1 (de) | Vorrichtung und Verfahren zur Bestimmung von in Proben enthaltenen chemischen oder biochemischen Partnern allgemeiner Rezeptor-Ligand-Systeme |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM01 | Lapse because of not paying annual fees |
Effective date: 20230412 |