AR241546A1 - Metodo para la recuperacion de alfa amilasa o proteasa en forma cristalina y el producto de enzima cristalina resultante. - Google Patents

Metodo para la recuperacion de alfa amilasa o proteasa en forma cristalina y el producto de enzima cristalina resultante.

Info

Publication number
AR241546A1
AR241546A1 AR86303178A AR30317886A AR241546A1 AR 241546 A1 AR241546 A1 AR 241546A1 AR 86303178 A AR86303178 A AR 86303178A AR 30317886 A AR30317886 A AR 30317886A AR 241546 A1 AR241546 A1 AR 241546A1
Authority
AR
Argentina
Prior art keywords
recover
crystalline enzymes
enzyme
produced
enzymes
Prior art date
Application number
AR86303178A
Other languages
English (en)
Original Assignee
Miles Lab Inc Una Corpor Org C
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Lab Inc Una Corpor Org C filed Critical Miles Lab Inc Una Corpor Org C
Application granted granted Critical
Publication of AR241546A1 publication Critical patent/AR241546A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/836—Bacillus licheniformis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

UN METODO PARA LA RECUPERACION DE UNA ENZIMA ELEGIDA ENTRE ALFA AMILASA O PROTEASA A PARTIR DE UNA SOLUCION DE LA MISMA OBTENIDA DE UN MICROORGANISMO, POR EJEMPLO BACILLUS LICHENIFORMIS CAPAZ DESEGREGAR UNA O AMBAS DE DICHAS ENZIMAS, QUE COMPRENDE: (A) CONCENTRAR A SUPERSATURACION DE LA SOLUCION DE LA ENZIMA, MEDIANTE ULTRAFILTRACION Y UNA POSTERIOR EVAPORACION BAJO VACIO (OPCIONAL) ENTRE 25 A 39°C, TENIENDO UN PH DE +/- 2,5 UNIDADES RESPECTO AL PUNTO ISOELECTRICO DE LA ENZIMA, (B) INDUCIR LA CRISTALIZACION SINO SE HA PRODUCIDO DESPUES DE LA ULTRACENTRIFUGACION MEDIANTE AGITACION A UNA TEMPERATURA ENTRE 5 Y 60°C Y (C) RECUPERAR EL PRODUCTO CRISTALINO.
AR86303178A 1985-02-26 1986-02-19 Metodo para la recuperacion de alfa amilasa o proteasa en forma cristalina y el producto de enzima cristalina resultante. AR241546A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70555685A 1985-02-26 1985-02-26
US06/735,971 US4659667A (en) 1985-02-26 1985-05-20 Process to recover crystalline enzymes and crystalline enzymes produced thereby

Publications (1)

Publication Number Publication Date
AR241546A1 true AR241546A1 (es) 1992-08-31

Family

ID=27107530

Family Applications (1)

Application Number Title Priority Date Filing Date
AR86303178A AR241546A1 (es) 1985-02-26 1986-02-19 Metodo para la recuperacion de alfa amilasa o proteasa en forma cristalina y el producto de enzima cristalina resultante.

Country Status (7)

Country Link
US (1) US4659667A (es)
EP (1) EP0193046B1 (es)
AR (1) AR241546A1 (es)
CA (1) CA1262878A (es)
DE (1) DE3682411D1 (es)
DK (1) DK163936C (es)
MX (1) MX163250B (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988008031A1 (en) * 1987-04-08 1988-10-20 Genetics Institute, Inc. Process for preparing cyclodextrins
JPH02502786A (ja) * 1987-12-23 1990-09-06 ギスト　ブロカデス　ナームローゼ　フェンノートチャップ 精製された工業酵素及びその調製方法
US5120650A (en) * 1989-10-13 1992-06-09 Stabra Ag Method for producing crystalline glucose isomerase
DK173590D0 (da) * 1990-06-06 1990-07-19 Novo Nordisk As Rekombinante terapeutiske lipaser
US5281526A (en) * 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
DK39693D0 (da) * 1993-04-02 1993-04-02 Novo Nordisk As Enzym
EP0795009A1 (en) * 1994-12-28 1997-09-17 Genencor International Inc. Enzyme stabilization
US6207437B1 (en) 1996-03-11 2001-03-27 Genencor International, Inc. Crystalline protease and method for producing same
US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6140475A (en) * 1997-04-11 2000-10-31 Altus Biologics Inc. Controlled dissolution crosslinked protein crystals
WO1999017107A2 (de) * 1997-09-26 1999-04-08 Siemens Aktiengesellschaft Mikrostrukturierter biosensor, verwendung des biosensors und verfahren zur immobilisierung von biokatalysatoren
CA2361507C (en) * 1999-03-05 2014-05-06 Genencor International, Inc. Method for rapidly obtaining crystals with desirable morphologies
US20010046493A1 (en) * 2000-02-24 2001-11-29 Alex Margolin Lipase-containing composition and methods of use thereof
WO2003050274A2 (en) * 2001-12-11 2003-06-19 Novozymes A/S Process for harvesting crystalline particles from fermentation broth
US7118891B2 (en) * 2001-12-11 2006-10-10 Novozymes A/S Crystal harvest from fermentation broth
EP1809320B1 (en) * 2004-10-14 2010-07-21 Altus Pharmaceuticals Inc. Compositions containing lipase; protease and amylase for treating pancreatic insufficiency
US20100196344A1 (en) * 2005-10-14 2010-08-05 Cystic Fibrosis Foundation Therapeutics, Inc. Compositions and methods for treating pancreatic insufficiency
EP2464446B1 (en) 2009-08-10 2014-12-24 Danisco US Inc. Cross-flow membrane filtration-based process for protein recovery
EP3858145A1 (en) * 2016-12-23 2021-08-04 Arla Foods amba Production of novel beta-lactoglobulin preparations and related methods, uses, and food products

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU495347A1 (ru) * 1973-07-30 1975-12-15 Всесюзный Научно-Исследовательский Институт Биосинтеза Белковых Веществ Способ выделени ферментов из растворов
JPS5362891A (en) * 1976-11-12 1978-06-05 Niigata Engineering Co Ltd Enzyme purifying and concentrating method
DD202319B3 (de) * 1980-12-23 1992-07-16 Inst Enzymologie & Tech Verfahren zur herstellung von stabilen hochkonzentrierten alpha-amylasefluessigpraeparaten

Also Published As

Publication number Publication date
EP0193046A2 (en) 1986-09-03
DK163936C (da) 1992-09-21
DK87286A (da) 1986-08-27
CA1262878C (en) 1989-11-14
DE3682411D1 (de) 1991-12-19
DK87286D0 (da) 1986-02-25
DK163936B (da) 1992-04-21
EP0193046B1 (en) 1991-11-13
CA1262878A (en) 1989-11-14
EP0193046A3 (en) 1988-07-20
US4659667A (en) 1987-04-21
MX163250B (es) 1992-03-24

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