MX173840B - Procedimiento para producir una enzima de alfa-amilasa - Google Patents

Procedimiento para producir una enzima de alfa-amilasa

Info

Publication number
MX173840B
MX173840B MX025687A MX2568786A MX173840B MX 173840 B MX173840 B MX 173840B MX 025687 A MX025687 A MX 025687A MX 2568786 A MX2568786 A MX 2568786A MX 173840 B MX173840 B MX 173840B
Authority
MX
Mexico
Prior art keywords
alpha
amylase
producing
approximately
amylase enzyme
Prior art date
Application number
MX025687A
Other languages
English (en)
Inventor
Nancy W Zeman
Original Assignee
Cpc International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cpc International Inc filed Critical Cpc International Inc
Publication of MX173840B publication Critical patent/MX173840B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/842—Clostridium

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a procedimeinto para producir una enzima de alfa-amilasa que deriva de clostridium thermohydrosulfuricum, teniendo dicha enzima un peso molecular de aproximadamente 72.000+-3.000 determinado por electroforesis de gel de poliacrilamida-SDS, que muestra una vida media de aproximadamente 450 minutos cuando se mantienen a 80ºC y a un pH 4,5 en presencia de 5 mM Ca++, que tiene una atividad máxina de alfa-amilasa a un pH de aproximadamente 5,5 cuando se mide a 80ºC, y que tiene una actividad máxima de alfa-amilasa a un pH de 5,0 a aproximadamente 60ºC; caracterizado por las etapas de cultivar celulas de clostridium thermohydrosulfuricumen un medio nutriente y aislar luego la enzima de alfa-amilasa del medio.
MX025687A 1985-02-08 1986-02-07 Procedimiento para producir una enzima de alfa-amilasa MX173840B (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/699,772 US4613570A (en) 1985-02-08 1985-02-08 Novel thermostable, aciduric alpha-amylase and method for its production

Publications (1)

Publication Number Publication Date
MX173840B true MX173840B (es) 1994-03-30

Family

ID=24810850

Family Applications (2)

Application Number Title Priority Date Filing Date
MX025687A MX173840B (es) 1985-02-08 1986-02-07 Procedimiento para producir una enzima de alfa-amilasa
MX1487A MX163267B (es) 1985-02-08 1986-02-07 Procedimiento mejorado para hidrolizar almidon

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX1487A MX163267B (es) 1985-02-08 1986-02-07 Procedimiento mejorado para hidrolizar almidon

Country Status (7)

Country Link
US (1) US4613570A (es)
JP (1) JPS61185185A (es)
DE (1) DE3603348A1 (es)
DK (1) DK60386A (es)
FR (1) FR2577237B1 (es)
MX (2) MX173840B (es)
NL (1) NL8600286A (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61115496A (ja) * 1984-11-09 1986-06-03 Hitachi Ltd 殿粉の液化方法
US4778760A (en) * 1984-11-09 1988-10-18 Hitachi, Ltd. Thermostable α-amylase-producing, thermophilic anaerobic bacteria, thermostable α-amylase and process for producing the same
FI81112C (fi) * 1986-08-27 1990-09-10 Alko Ab Oy Amylas av ny typ.
DE3639267A1 (de) * 1986-11-17 1988-09-22 Antranikian Garabed Verfahren zur foerderung der exkretion von amylolytischen enzymen aus bakterien sowie nach dem verfahren erhaltene enzyme
JP2542251B2 (ja) * 1987-07-17 1996-10-09 エンドレ モドロビッチ，アイバン 安定な一液性α―アミラ―ゼ検定用試薬
US5188956A (en) * 1988-07-01 1993-02-23 Showa Denka K.K. Thermostable amylase
US5010008A (en) * 1988-12-12 1991-04-23 Enzyme Bio-Systems Ltd. Stable liquid enzyme concentrate and process for its production
GB201716845D0 (en) 2017-10-13 2017-11-29 Green Biologics Ltd Process
CN108823185A (zh) * 2018-06-25 2018-11-16 安徽新熙盟生物科技有限公司 高酶活发酵液的培养方法及提取耐酸α-淀粉酶的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4284722A (en) * 1978-08-16 1981-08-18 Cpc International Inc. Heat and acid-stable alpha-amylase enzymes and processes for producing the same
US4578352A (en) * 1983-07-13 1986-03-25 Cpc International Inc. Novel thermostable, aciduric alpha-amylase and method for its production
US4536477A (en) * 1983-08-17 1985-08-20 Cpc International Inc. Thermostable glucoamylase and method for its production
ES8602115A1 (es) * 1983-10-11 1985-11-01 Espanola Petrol Procedimiento para producir -amilasas termoestables por cultivo de microorganismos a altas temperaturas
US4778760A (en) * 1984-11-09 1988-10-18 Hitachi, Ltd. Thermostable α-amylase-producing, thermophilic anaerobic bacteria, thermostable α-amylase and process for producing the same

Also Published As

Publication number Publication date
FR2577237A1 (fr) 1986-08-14
JPS61185185A (ja) 1986-08-18
DK60386D0 (da) 1986-02-07
NL8600286A (nl) 1986-09-01
DE3603348A1 (de) 1986-08-28
FR2577237B1 (fr) 1989-12-22
MX163267B (es) 1992-03-31
DK60386A (da) 1986-08-09
US4613570A (en) 1986-09-23

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