AR124664A1 - Amplificación isotérmica mediada por horquillas (lamp) en un medio en fase sólida - Google Patents
Amplificación isotérmica mediada por horquillas (lamp) en un medio en fase sólidaInfo
- Publication number
- AR124664A1 AR124664A1 ARP220100088A ARP220100088A AR124664A1 AR 124664 A1 AR124664 A1 AR 124664A1 AR P220100088 A ARP220100088 A AR P220100088A AR P220100088 A ARP220100088 A AR P220100088A AR 124664 A1 AR124664 A1 AR 124664A1
- Authority
- AR
- Argentina
- Prior art keywords
- lamp
- solid phase
- present disclosure
- reaction
- layer disposed
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La presente divulgación se refiere a conjuntos de reacción de amplificación isotérmica mediada por horquillas (LAMP) que incluyen una mezcla de reactivos LAMP sustancialmente libre de agentes higroscópicos en combinación con un medio de reacción en fase sólida. La presente divulgación también incluye sistemas para un análisis LAMP cromático que incluyen un medio de reacción en fase sólida sustancialmente no reactivo y una mezcla de reactivos no interferentes. La presente divulgación también incluye medios de reacción LAMP en fase sólida que comprenden un sustrato, una capa adhesiva dispuesta sobre el sustrato, una capa de reacción dispuesta sobre la capa adhesiva, y una capa de extensión dispuesta sobre la capa de reacción. La presente divulgación también incluye métodos de ensayo para detectar la presencia de una secuencia de nucleótidos objetivo que incluyen proporcionar una muestra biológica, y dispensar la muestra en un entorno de prueba que tiene un medio de reacción en fase sólida en combinación con una mezcla de reactivos LAMP y un colorante sensible al pH.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163138321P | 2021-01-15 | 2021-01-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR124664A1 true AR124664A1 (es) | 2023-04-19 |
Family
ID=81025318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220100088A AR124664A1 (es) | 2021-01-15 | 2022-01-17 | Amplificación isotérmica mediada por horquillas (lamp) en un medio en fase sólida |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP4278003A1 (es) |
| AR (1) | AR124664A1 (es) |
| TW (1) | TW202246524A (es) |
| WO (1) | WO2022155550A1 (es) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3015368A1 (en) * | 2016-03-14 | 2017-09-21 | Diassess Inc. | Systems and methods for performing biological assays |
| CN114650883B (zh) * | 2019-06-18 | 2023-10-27 | 猛犸生物科学公司 | 用于检测核酸的测定和方法 |
| EP4118233A1 (en) * | 2020-03-12 | 2023-01-18 | New England Biolabs, Inc. | A rapid diagnostic test for lamp |
-
2022
- 2022-01-14 TW TW111101760A patent/TW202246524A/zh unknown
- 2022-01-15 WO PCT/US2022/012640 patent/WO2022155550A1/en unknown
- 2022-01-15 EP EP22704038.3A patent/EP4278003A1/en active Pending
- 2022-01-17 AR ARP220100088A patent/AR124664A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022155550A1 (en) | 2022-07-21 |
| TW202246524A (zh) | 2022-12-01 |
| EP4278003A1 (en) | 2023-11-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |