WO2019038706A3 - Paper-based molecular diagnostic device and uses thereof - Google Patents

Paper-based molecular diagnostic device and uses thereof Download PDF

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Publication number
WO2019038706A3
WO2019038706A3 PCT/IB2018/056384 IB2018056384W WO2019038706A3 WO 2019038706 A3 WO2019038706 A3 WO 2019038706A3 IB 2018056384 W IB2018056384 W IB 2018056384W WO 2019038706 A3 WO2019038706 A3 WO 2019038706A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
paper
paper layer
measurement
reading
Prior art date
Application number
PCT/IB2018/056384
Other languages
French (fr)
Other versions
WO2019038706A2 (en
Inventor
Feng Li
Guan Wang
Tianyu DONG
Original Assignee
Brock University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brock University filed Critical Brock University
Publication of WO2019038706A2 publication Critical patent/WO2019038706A2/en
Publication of WO2019038706A3 publication Critical patent/WO2019038706A3/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/028—Graduation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/126—Paper

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The disclosure provides a paper-based nucleic acid detecting device and measurement method. This device comprises paper layer set with pattern. This paper layer comprises a sample loading zone and a test zone. Herein, the pattern on paper layer indicates the measurement of nucleic acid by the nucleic acid detecting device. This measuring method can perform quantitative analysis of nucleic acid molecules by reading the retention distance of the chromogenic substance, the colored substance or the fluorescent substance. The device and measurement method of this disclosure can achieve rapid and accurate measurement of nucleic acid by reading the test result of the test sample.
PCT/IB2018/056384 2017-08-24 2018-08-23 Paper-based molecular diagnostic device and uses thereof WO2019038706A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201710737730.3 2017-08-24
CN201710737730.3A CN107384769B (en) 2017-08-24 2017-08-24 Paper-based nucleic acid detection device and application thereof

Publications (2)

Publication Number Publication Date
WO2019038706A2 WO2019038706A2 (en) 2019-02-28
WO2019038706A3 true WO2019038706A3 (en) 2019-04-18

Family

ID=60345927

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2018/056384 WO2019038706A2 (en) 2017-08-24 2018-08-23 Paper-based molecular diagnostic device and uses thereof

Country Status (2)

Country Link
CN (1) CN107384769B (en)
WO (1) WO2019038706A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109254043B (en) * 2018-10-29 2020-10-02 济南大学 Preparation of automatic cleaning paper-based sensing device and application of automatic cleaning paper-based sensing device in ion analysis
CN110265089B (en) * 2019-06-20 2021-11-09 北京科技大学 Nucleic acid quantitative analysis method based on assistance of intelligent equipment and application thereof
CN110452813A (en) * 2019-06-28 2019-11-15 马鞍山众慧惠生生物科技有限公司 A kind of sliding nucleic acid detection apparatus
CN110358667A (en) * 2019-06-28 2019-10-22 马鞍山众慧惠生生物科技有限公司 A kind of Multi-example sliding nucleic acid detection apparatus
CN114341627A (en) * 2019-11-13 2022-04-12 李峰 Nucleic acid detection method and device
CN111088155A (en) * 2019-12-30 2020-05-01 航天神舟生物科技集团有限公司 PCR detection microfluidic paper chip and preparation method and application thereof
CN111693694B (en) * 2020-05-19 2023-02-17 江苏大学 Multi-virus immunodetection device and method based on smart phone and micro-fluidic chip
CN111575167A (en) * 2020-05-25 2020-08-25 广州新诚生物科技有限公司 Nucleic acid detection equipment and use method thereof
CN112285078B (en) * 2020-10-14 2023-04-25 安庆师范大学 Novel method for detecting mercury ions on site based on intelligent DNA hydrogel

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104004850A (en) * 2014-06-11 2014-08-27 华南师范大学 Paper-based micro-fluidic chip enhancement type chemiluminescence gene sensing method
WO2016064881A1 (en) * 2014-10-24 2016-04-28 Abbott Laboratories Paper substrate diagnostic apparatus and related methods and systems

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101818200A (en) * 2010-03-04 2010-09-01 宁波博奥生物工程有限公司 Wound vibrio quantitative detecting test strip
CN103389303A (en) * 2013-08-07 2013-11-13 苏州扬清芯片科技有限公司 Multi-index analysis paper chip and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104004850A (en) * 2014-06-11 2014-08-27 华南师范大学 Paper-based micro-fluidic chip enhancement type chemiluminescence gene sensing method
WO2016064881A1 (en) * 2014-10-24 2016-04-28 Abbott Laboratories Paper substrate diagnostic apparatus and related methods and systems

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NOOR , O. ET AL.: "Paper-Based Solid-Phase Multiplexed Nucleic Acid Hybridization Assay with Tunable Dynamic Range Using Immobilized Quantum Dots As Donors in Fluorescence Resonance Energy Transfer", ANALYTICAL CHEMISTRY, vol. 85, 9 July 2013 (2013-07-09), pages 7502 - 7511, XP055470961, ISSN: 0003-2700, DOI: doi:10.1021/ac401471n *
SEOK, Y. ET AL.: "A Paper-Based Device for Performing Loop-Mediated Isothermal Amplification with Real-Time Simultaneous Detection of Multiple DNA Targets", THERANOSTICS, vol. 7, no. 8, 1 June 2017 (2017-06-01), pages 2220 - 2230, XP055592420, ISSN: 1838-7640 *

Also Published As

Publication number Publication date
CN107384769A (en) 2017-11-24
WO2019038706A2 (en) 2019-02-28
CN107384769B (en) 2021-04-09

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