CN110452813A - A kind of sliding nucleic acid detection apparatus - Google Patents
A kind of sliding nucleic acid detection apparatus Download PDFInfo
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- CN110452813A CN110452813A CN201910576767.1A CN201910576767A CN110452813A CN 110452813 A CN110452813 A CN 110452813A CN 201910576767 A CN201910576767 A CN 201910576767A CN 110452813 A CN110452813 A CN 110452813A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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Abstract
A kind of sliding nucleic acid detection apparatus, including bottom plate；Fixing seat is set on the bottom plate, and fixing seat is equipped with sliding slot, and one end of sliding slot is equipped with blood filter device, and for filtering blood to obtain serum, the other end of sliding slot is equipped with thermostat, for providing constant temperature when serum nucleic acid expands；Slide bar, sliding bar are connected to sliding slot, and one end of slide bar is equipped with coupongs, for obtaining the filtered serum of blood filter device；Reagent adding set is set on sliding slot, for adding reagent to coupongs；Driving device is slided set on the other end of slide bar for driving slider；Fluorescence reading device carries out fluorescence reading for the nucleic acid amplification result to coupongs.A kind of sliding nucleic acid detection apparatus of the present invention, realizes the extraction and amplification of sample of nucleic acid by way of sliding, and to can amplification detect, it is at low cost and easily operated, it is easy to carry.
Description
Technical field
The invention belongs to paper base care diagnostic fields, more particularly, to a kind of sliding nucleic acid detection apparatus.
Background technique
Detection of nucleic acids is widely used in fields such as medical diagnosis on disease, environmental monitoring, food safeties.Its detection process
It mainly include three steps: nucleic acid extraction, nucleic acid amplification, detection of nucleic acids.Traditional detection method depends on laboratory large size
Instrument analysis, such as PCR instrument, genome sequencing system.These methods have the characteristics that highly sensitive, high accuracy, still
Its testing cost is high, detection time is long, needs professional test personnel, thus these methods be difficult to promote the use of family or
In community environment.
With the propulsion of accurate medical treatment and classification diagnosis and treatment policy, field of nucleic acid detection shows low cost, minimizes, is portable
Formula, integrated trend.Therefore more and more researchs concentrate on the exploitation of miniaturization, integrated nucleic acid detection apparatus.Due to
Paper has the physical characteristics such as low cost and hydrophily, porosity, presented above huge out in portable, integrated device exploitation
Big potentiality.It is easy to carry therefore, it is necessary to research and develop a kind of portable nucleic acid acquisition amplification device.
Summary of the invention
The object of the present invention is to provide a kind of sliding nucleic acid detection apparatus, nucleic acid extraction can be integrated and expanded in one,
It is easily operated, it is easy to carry.
To achieve the goals above, the present invention provides a kind of sliding nucleic acid detection apparatus, comprising:
Bottom plate；
Fixing seat is set on the bottom plate, and the fixing seat is equipped with sliding slot, and one end of the sliding slot is equipped with blood filtration
Device, for filtering blood to obtain serum, the other end of the sliding slot is equipped with thermostat, in serum nucleic acid amplification
Constant temperature is provided；
Slide bar, the sliding bar are connected to the sliding slot, and one end of the slide bar is equipped with coupongs, described for obtaining
The filtered serum of blood filter device；
Reagent adding set is set on the sliding slot, for adding reagent to the coupongs；
Driving device is set on the bottom plate, and is located at the other end of the slide bar, for driving the sliding bar；
Fluorescence reading device carries out fluorescence reading for the nucleic acid amplification result to the coupongs.
Preferably, described one end of the sliding slot is equipped with linking arm, and the linking arm is equipped with sample aperture, the blood mistake
It filters device to be set in the sample aperture, drip blood can by the sample aperture after blood filter device filtering
Under.
Preferably, the blood filter device is blood filter membrane, in the blood filter membrane covering and the sample aperture.
Preferably, the coupongs are paper chip or FTA card, and the reagent having for extracting DNA is added dropwise in the paper chip.
Preferably, the sliding slot is equipped with first position and the second position, and the reagent adding set includes the first dropwise addition
Pad is added dropwise in pad and second, and each described one end that pad is added dropwise is connected on one side wall of the sliding slot, other end difference
It is suspended on above first position and the second position；Each dropwise addition pad, which can turn down, takes the other end with described
Print contact.
Preferably, the reagent adding set further includes the first water absorption pad and the second water absorption pad, first water absorption pad with
Described first, which is added dropwise pad, second water absorption pad and the second dropwise addition pad, is relatively arranged on the side wall of the sliding slot, is used for
Draw liquid extra on the coupongs.
Preferably, the driving device includes driving wheel, drive shaft and driving band, and one end of the driving band is around in described
On driving wheel, the other end of the driving band is connected to the other end of the slide bar, and the drive shaft is connected to the driving wheel
And by being rotationally connected with the bottom plate, the drive shaft turns drive the driving wheel rotation, to make the driving band water
Translation is dynamic.
Preferably, the distance between the blood filter device and the first position, the first position and described the
The distance between two positions and the distance between the second position and the thermostat are the perimeter of the driving wheel
Equimultiple.
Preferably, the thermostat includes controller, heater and temperature sensor, the other end of the sliding slot
For " returning " type structure, the slide bar passes through the center of described " going back to " type, and the heater is set to the top and bottom of described " going back to " type
Portion, the temperature sensor are set to the side wall of described " returning " type, and the controller is according to the temperature signal of the temperature sensor
Control the start and stop of the heater.
Preferably, the fluorescence reading device includes light source and photographing device, and the light source is for mentioning the coupongs
For illumination, the photographing device is used to obtain image information to the coupongs under illumination.
A kind of sliding nucleic acid detection apparatus of the present invention, the beneficial effect is that: core is realized by way of sliding
The extraction and amplification of acid sample, and to can amplification detect, it is at low cost and easily operated, it is easy to carry.
Other features and advantages of the present invention will then part of the detailed description can be specified.
Detailed description of the invention
Exemplary embodiment of the invention is described in more detail in conjunction with the accompanying drawings, it is of the invention above-mentioned and its
Its purpose, feature and advantage will be apparent, wherein in exemplary embodiment of the invention, identical reference label
Typically represent same parts.
Fig. 1 shows the sliding nucleic acid detection apparatus structural schematic diagram of one exemplary embodiment of the present invention；
Fig. 2 a, Fig. 2 b and Fig. 2 c are respectively illustrated in the sliding nucleic acid detection apparatus of one exemplary embodiment of the present invention
The sample detection result of difference nucleic acid concentration when coupongs are paper chip；
Description of symbols:
1 bottom plate, 2 fixing seats, 3 sliding slots, 4 thermostats, 5 blood filter devices, 6 slide bars, 7 coupongs, 8 driving devices, 9
Linking arm, 10 driving wheels, 11 drive shafts, 12 driving bands, 13 first are added dropwise pad, and 14 second are added dropwise pad, 15 first water absorption pads, and 16 the
Two water absorption pads.
Specific embodiment
The preferred embodiment of the present invention is described in more detail below.Although the following describe preferred implementations of the invention
Mode, however, it is to be appreciated that may be realized in various forms the present invention without that should be limited by the embodiments set forth herein.Phase
Instead, these embodiments are provided so that the present invention is more thorough and complete, and can be by the scope of the present invention completely
It is communicated to those skilled in the art.
In order to solve the problems existing in the prior art, the present invention provides a kind of sliding nucleic acid detection apparatus, comprising:
Bottom plate；
Fixing seat is set on the bottom plate, and fixing seat is equipped with sliding slot, and one end of sliding slot is equipped with blood filter device, was used for
Hemofiltration liquid is to obtain serum, and the other end of sliding slot is equipped with thermostat, for providing constant temperature when serum nucleic acid expands；
Slide bar, sliding bar are connected to sliding slot, and one end of slide bar is equipped with coupongs, for obtaining blood filter device filtering
Serum afterwards；
Reagent adding set is set on sliding slot, for adding reagent to coupongs；
Driving device is set on the bottom plate, and is located at the other end of slide bar, is slided for driving slider；
Fluorescence reading device carries out fluorescence reading for the nucleic acid amplification result to coupongs.
A kind of sliding nucleic acid detection apparatus of the present invention, realizes the extraction and expansion of sample of nucleic acid by way of sliding
Increase, and to can amplification detect, it is at low cost and easily operated, it is easy to carry.
Preferably, one end of sliding slot is equipped with linking arm, and linking arm is equipped with sample aperture, and blood filter device is set to sample aperture
On, make blood that can drip by sample aperture after blood filter device filters.Linking arm is connected to the two side walls of sliding slot,
Coupongs on slide bar can slide into the underface of sample aperture, to obtain filtered serum.
Preferably, blood filter device is blood filter membrane, blood filter membrane covering in sample aperture, in filtering blood sample
Red blood cell, to obtain serum.
Preferably, coupongs are paper chip or FTA card, the reagent having for extracting DNA are added dropwise in paper chip, by filtering
Serum afterwards is dropped to and is permeated in paper chip, and the extraction of DNA is completed by reagent；The honeycomb cell structure of paper chip fixes DNA；
FTA card is market existing product, has the function that DNA is extracted in serum, extracts reagent without adding DNA.
Preferably, sliding slot is equipped with first position and the second position, and reagent adding set includes the first dropwise addition pad and second
Pad is added dropwise, each one end that pad is added dropwise is connected on a side wall of sliding slot, and the other end is suspended on first position and second respectively
Above position, i.e., first other end that pad is added dropwise is located above first position, and second other end that pad is added dropwise is located at the second position
Top；Each dropwise addition pad, which can turn down, contacts the other end with coupongs.Each other end that pad is added dropwise is small rectangle, when
When coupongs successively move to first position and the second position under the sliding of slide bar, each pad that is added dropwise being capable of side wall along the chute
Side fold contacts small rectangular end with coupongs, reagent is added dropwise.
Preferably, being added dropwise on the first dropwise addition pad has pure water, for rinsing the impurity in paper chip；It is added dropwise on second dropwise addition pad
There is amplifing reagent, for expanding to the DNA nucleic acid on coupongs.
Preferably, reagent adding set further includes the first water absorption pad and the second water absorption pad, and the first water absorption pad and first are added dropwise
Pad, the second water absorption pad and second are added dropwise pad and are relatively arranged on the side wall of sliding slot, for drawing liquid extra on coupongs.
Preferably, another top side wall of sliding slot, the first water suction are fixed in one end of the first water absorption pad and the second water absorption pad
Pad hanging with the other end of the second water absorption pad, and be small rectangle, and the side for capableing of another side wall along the chute turn down to sampling
Piece contact.
When coupongs are when the sliding of slide bar is displaced downwardly to first position, the pure water that fold first is added dropwise pad and makes thereon, which drops to, to be taken
On print, the first water absorption pad of fold draws liquid remaining on coupongs, is rinsed the impurity on coupongs with this；Work as coupongs
When sliding into the second position, second, which is added dropwise pad fold, drops to the amplifing reagent on small rectangular end on coupongs, and fold second is inhaled
Water cushion draws liquid remaining on coupongs.
Preferably, driving device includes driving wheel, drive shaft and driving band, drives one end of band to be around on driving wheel, drives
The other end of dynamic band is connected to the other end of slide bar, and drive shaft is connected to driving wheel and by being rotationally connected with bottom plate, drive shaft
Rotation drives driving wheel rotation, to make that band is driven to move horizontally.
Preferably, drive shaft is rotationally connected on bottom plate by bearing block, and one end of drive shaft is fixedly connected with driving wheel,
The other end is equipped with handle, and swing handle makes drive shaft turns, so that driving wheel be made to rotate.
Preferably, the distance between the distance between blood filter device and first position, first position and the second position,
And the distance between the second position and thermostat are the equimultiple of the perimeter of driving wheel, driving wheel rotation one is enclosed or a few, i.e.,
Slide bar can be made to be moved to postorder position by preamble position, thus function when completing to be located at each position.
Preferably, thermostat includes controller, heater and temperature sensor, and the other end of sliding slot is " returning " type knot
Structure, slide bar pass through the center of " going back to " type, and heater is set to the top and bottom of " returning " type, and temperature sensor is set to the side of " going back to " type
Wall, controller control the start and stop of heater according to the temperature signal of temperature sensor to keep constant temperature.When coupongs slide into
When among " returning " type structure, starting heater is heated, and reaches constant temperature, to guarantee that DNA nucleic acid smoothly expands.
Preferably, fluorescence reading device includes light source and photographing device, and light source is used to provide illumination to coupongs, takes pictures and set
It is ready for use on and image information is obtained to the coupongs under illumination.After photographing device obtains image information, i.e. the expansion of observable DNA nucleic acid
Increase as a result, i.e. testing result, to carry out quantitative analysis.
Preferably, light source is ultraviolet lamp, mercury lamp, xenon lamp, LED or laser.
Embodiment 1
As shown in Fig. 1 to Fig. 2 c, the present invention provides a kind of sliding nucleic acid detection apparatus, comprising:
Bottom plate 1；
Fixing seat 2 is set on bottom plate 1, and fixing seat 2 is equipped with sliding slot 3, and one end of sliding slot 3 is equipped with blood filter device 5,
For filtering blood to obtain serum, the other end of sliding slot 3 is equipped with thermostat 4, permanent for providing in serum nucleic acid amplification
Temperature；
Slide bar 6, slide bar 6 are slidably connected to sliding slot 3, and one end of slide bar 6 is equipped with coupongs 7, for obtaining blood filtration dress
Set 5 filtered serum；
Reagent adding set is set on sliding slot 3, for adding reagent to coupongs 7；
Driving device 8 is set on bottom plate 1, and is located at the other end of slide bar 6, is slided for driving slider 6；
Fluorescence reading device carries out fluorescence reading for the nucleic acid amplification result to coupongs 7.
In the present embodiment, one end of sliding slot 3 is equipped with linking arm 9, and linking arm 9 is equipped with sample aperture, blood filter device 5
In sample aperture, make blood that can drip by sample aperture after the filtering of blood filter device 5.Linking arm 9 is connected to cunning
The two side walls of slot 3, the coupongs 7 on slide bar 6 can slide into the underface of sample aperture, to obtain filtered serum.
In the present embodiment, blood filter device 5 be blood filter membrane, blood filter membrane covering with sample aperture on.
In the present embodiment, coupongs 7 are paper chip, the reagent having for extracting DNA are added dropwise in paper chip, by filtering
Serum afterwards is dropped to and is permeated in paper chip, and the extraction of DNA is completed by reagent；The honeycomb cell structure of paper chip fixes DNA.
In the present embodiment, sliding slot 3 is equipped with first position and the second position, and reagent adding set includes the first dropwise addition pad
13 and second are added dropwise pad 14, and each one end that pad is added dropwise is connected on a side wall of sliding slot 3, and the other end is suspended on first respectively
Above position and the second position, i.e., the other end of the first dropwise addition pad 13 is located above first position, and second is added dropwise the another of pad 14
End is located above the second position；Each dropwise addition pad, which can turn down, contacts the other end with coupongs 7.It is each that the another of pad is added dropwise
End is small rectangle, when successively moving to first position and the second position under sliding of the coupongs 7 in slide bar 6, each dropwise addition pad
Can along the chute 3 side walls side fold contact small rectangular end with coupongs 7, reagent is added dropwise.
In the present embodiment, being added dropwise on the first dropwise addition pad 13 has pure water, for rinsing the impurity in paper chip；Second is added dropwise
Being added dropwise on pad 14 has amplifing reagent, for expanding to the DNA nucleic acid on coupongs 7.
In the present embodiment, reagent adding set further includes the first water absorption pad 15 and the second water absorption pad 16, the first water absorption pad
15 and the first dropwise addition pad 13, the second water absorption pad 16 pad 14 is added dropwise with second and is relatively arranged on the side wall of sliding slot 3, for taking
Extra liquid on print 7.Another top side wall of sliding slot 3 is fixed in one end of first water absorption pad 15 and the second water absorption pad 16,
The other end of first water absorption pad 15 and the second water absorption pad 16 is hanging, and is small rectangle, and be capable of the side of 3 another side wall along the chute
Fold is extremely contacted with coupongs 7.
When coupongs 7 are when the sliding of slide bar 6 is displaced downwardly to first position, fold the first dropwise addition pad 13 drips pure water thereon
To coupongs 7, the first water absorption pad 15 of fold draws liquid remaining on coupongs 7, is rushed the impurity on coupongs 7 with this
It washes；When coupongs 7 slide into the second position, the second dropwise addition fold of pad 14 makes the amplifing reagent on small rectangular end drop to coupongs
On 7, the second water absorption pad 16 of fold draws liquid remaining on coupongs 7.
In the present embodiment, driving device 8 includes driving wheel 10, drive shaft 11 and driving band 12, drives one end of band 12
It is around on driving wheel 10, the other end of driving band 12 is connected to the other end of slide bar 6, and drive shaft 11 is connected to driving wheel 10 and leads to
It crosses and is rotationally connected with bottom plate 1, the rotation of drive shaft 11 drives driving wheel 10 to rotate, to make that band 12 is driven to move horizontally.Drive shaft
11 are rotationally connected on bottom plate 1 by bearing block, and one end of drive shaft 11 is fixedly connected with driving wheel 10, and the other end is equipped with handle
Hand, swing handle rotate drive shaft 11, so that driving wheel 10 be made to rotate.
In the present embodiment, between the distance between blood filter device 5 and first position, first position and the second position
Distance and the distance between the second position and thermostat 4 be driving wheel 10 perimeter equimultiple, driving wheel 10 rotation one
Circle or a few, slide bar 6 can be made to be moved to postorder position by preamble position, thus complete be located at each position when function.
In the present embodiment, thermostat 4 includes controller, heater and temperature sensor, and the other end of sliding slot 3 is
" returning " type structure, slide bar 6 pass through the center of " going back to " type, and heater is set to the top and bottom of " returning " type, and temperature sensor is set to
The side wall of " returning " type, controller control the start and stop of heater according to the temperature signal of temperature sensor to keep constant temperature.Work as sampling
When piece 7 is slid among " returning " type structure, starting heater is heated, and reaches constant temperature, to guarantee that DNA nucleic acid smoothly expands.
In the present embodiment, fluorescence reading device includes light source and photographing device, and light source is used to provide light to coupongs 7
According to photographing device is used to obtain image information to the coupongs 7 under illumination.After photographing device obtains image information, i.e. observable
The amplification of DNA nucleic acid, i.e. testing result, to carry out quantitative analysis.Light source is laser.
The process for carrying out detection of nucleic acids using the sliding nucleic acid detection apparatus in the present embodiment is as follows:
DNA extractant is added dropwise on coupongs 7, is used after to be dried；Blood of human body sample is added dropwise in blood filter device 5
On, for drippage on coupongs 7, standing five minutes is fixed on DNA on coupongs 7 after the serum after red blood cell filtration；It is logical
Over-driving device 8 makes coupongs 7 be moved to first position, and pure water is added dropwise and is added dropwise on pad 13 in first, pad 13 is added dropwise in fold first
It drops to pure water on coupongs 7 and rinses impurity, and turn down the first water absorption pad 15 and draw liquid extra on coupongs 7；Movement takes
Amplifing reagent is added dropwise on the second dropwise addition pad 14 to the second position in print 7, and turning down the second dropwise addition pad 14 is added dropwise amplifing reagent
To coupongs 7, the second water absorption pad 16 of fold draws liquid extra on coupongs 7；Mobile coupongs 7 are another to sliding slot 3
Coupongs 7 are placed on fluorescence reading device after opening the heating of thermostat 4 30 minutes and obtain fluorescent image by end.
The nucleic acid of three kinds of concentration is detected respectively, and as shown in Fig. 2 a to 2c, nucleic acid concentration is higher, acquired fluorescent image
Fluorescence intensity is higher, to further demonstrate the present apparatus for the quantitative analysis ability of nucleic acid, and operating process is simple, is not necessarily to
Professional person, convenient for promoting.
Various embodiments of the present invention are described above, above description is exemplary, and non-exclusive, and
It is not limited to disclosed each embodiment.Without departing from the scope and spirit of illustrated each embodiment, for this skill
Many modifications and changes are obvious for the those of ordinary skill in art field.
Claims (10)
1. a kind of sliding nucleic acid detection apparatus characterized by comprising
Bottom plate (1)；
Fixing seat (2) is set on the bottom plate (1), and the fixing seat (2) is equipped with sliding slot (3), one end of the sliding slot (3)
Equipped with blood filter device (5), for filtering blood to obtain serum, the other end of the sliding slot (3) is equipped with thermostat
(4), for providing constant temperature when serum nucleic acid expands；
Slide bar (6), the slide bar (6) are slidably connected to the sliding slot (2), and one end of the slide bar (6) is equipped with coupongs (7),
For obtaining the blood filter device (5) filtered serum；
Reagent adding set is set on the sliding slot (2), for adding reagent to the coupongs (5)；
Driving device (8) is set on the bottom plate (1), and is located at the other end of the slide bar (6), for driving the slide bar
(6) it slides；
Fluorescence reading device carries out fluorescence reading for the nucleic acid amplification result to the coupongs (7).
2. sliding nucleic acid detection apparatus according to claim 1, which is characterized in that described one end of the sliding slot, which is equipped with, to be connected
It connects arm (9), the linking arm (9) is equipped with sample aperture, and the blood filter device (5) is set in the sample aperture, makes blood
It can be dripped by the sample aperture after the blood filter device (5) filtering.
3. sliding nucleic acid detection apparatus according to claim 2, which is characterized in that the blood filter device (5) is filter
Blood film, blood filter membrane covering in the sample aperture.
4. sliding nucleic acid detection apparatus according to claim 1, which is characterized in that the coupongs (7) be paper chip or
The reagent having for extracting DNA is added dropwise in the paper chip for FTA card.
5. sliding nucleic acid detection apparatus according to claim 1, which is characterized in that the sliding slot (3) is equipped with first
It sets and the second position, the reagent adding set includes that pad (14), each dropwise addition is added dropwise in the first dropwise addition pad (13) and second
One end of pad is connected on one side wall of the sliding slot (3), and the other end is suspended on first position and the second position respectively
Top；Each dropwise addition pad, which can turn down, contacts the other end with the coupongs (7).
6. sliding nucleic acid detection apparatus according to claim 5, which is characterized in that the reagent adding set further includes
Pad (7), second suction is added dropwise in one water absorption pad (15) and the second water absorption pad (16), first water absorption pad (3) and described first
Water cushion (2) and the second dropwise addition pad (6) are relatively arranged on the side wall of the sliding slot (3), for drawing the coupongs (7)
Upper extra liquid.
7. sliding nucleic acid detection apparatus according to claim 5, which is characterized in that the driving device (8) includes driving
(10), drive shaft (11) and driving band (12) are taken turns, one end of the driving band (12) is around on the driving wheel (10), the drive
The other end of dynamic band (12) is connected to the other end of the slide bar (6), and the drive shaft (11) is connected to the driving wheel (10)
And by being rotationally connected with the bottom plate (1), drive shaft (11) rotation drives driving wheel (10) rotation, to make institute
Driving band (12) is stated to move horizontally.
8. sliding nucleic acid detection apparatus according to claim 5, which is characterized in that the blood filter device (5) and institute
State the distance between the distance between first position, the first position and described second position and the second position and institute
State the equimultiple that the distance between thermostat (4) is the perimeter of the driving wheel.
9. sliding nucleic acid detection apparatus according to claim 1, which is characterized in that the thermostat include controller,
Heater and temperature sensor, the other end of the sliding slot are " returning " type structure, and the slide bar (6) passes through described " returning " type
Center, the heater is set to the top and bottom of described " returning " type, the temperature sensor be set to described in " returning " type side
Wall, the controller control the start and stop of the heater according to the temperature signal of the temperature sensor.
10. sliding nucleic acid detection apparatus according to claim 1, which is characterized in that the fluorescence reading device includes light
Source and photographing device, the light source are used to provide the coupongs (7) illumination, and the photographing device is used for the institute under illumination
It states coupongs (7) and obtains image information.
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| CN201910576767.1A CN110452813A (en) | 2019-06-28 | 2019-06-28 | A kind of sliding nucleic acid detection apparatus |
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| CN201910576767.1A CN110452813A (en) | 2019-06-28 | 2019-06-28 | A kind of sliding nucleic acid detection apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226358A (en) * | 2020-12-11 | 2021-01-15 | 博奥生物集团有限公司 | Nucleic acid analysis card box and nucleic acid analysis equipment |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005295910A (en) * | 2004-04-13 | 2005-10-27 | Enplas Corp | Nucleic acid-extracting apparatus |
| US20140295415A1 (en) * | 2011-11-04 | 2014-10-02 | Diagnostics For All, Inc. | Low cost, disposable molecular diagnostic devices |
| US20160016166A1 (en) * | 2013-03-14 | 2016-01-21 | Diagnostics For All, Inc. | Molecular diagnostic devices with magnetic components |
| CN107384769A (en) * | 2017-08-24 | 2017-11-24 | 李峰 | Nucleic acid detection apparatus and its application based on paper |
-
2019
- 2019-06-28 CN CN201910576767.1A patent/CN110452813A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005295910A (en) * | 2004-04-13 | 2005-10-27 | Enplas Corp | Nucleic acid-extracting apparatus |
| US20140295415A1 (en) * | 2011-11-04 | 2014-10-02 | Diagnostics For All, Inc. | Low cost, disposable molecular diagnostic devices |
| US20160016166A1 (en) * | 2013-03-14 | 2016-01-21 | Diagnostics For All, Inc. | Molecular diagnostic devices with magnetic components |
| CN107384769A (en) * | 2017-08-24 | 2017-11-24 | 李峰 | Nucleic acid detection apparatus and its application based on paper |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226358A (en) * | 2020-12-11 | 2021-01-15 | 博奥生物集团有限公司 | Nucleic acid analysis card box and nucleic acid analysis equipment |
| CN112226358B (en) * | 2020-12-11 | 2021-03-23 | 博奥生物集团有限公司 | Nucleic acid analysis card box and nucleic acid analysis equipment |
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