CN209039485U - A kind of bio-chip test device - Google Patents
A kind of bio-chip test device Download PDFInfo
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- CN209039485U CN209039485U CN201821555153.2U CN201821555153U CN209039485U CN 209039485 U CN209039485 U CN 209039485U CN 201821555153 U CN201821555153 U CN 201821555153U CN 209039485 U CN209039485 U CN 209039485U
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Abstract
The utility model relates to biochip test apparatus fields, in particular to a kind of bio-chip test device, the bio-chip test device includes the cavity for carrying test tube bottle, the test tube bottle has function of temperature control for accommodating sample and biochip to be detected, the cavity；For storing the device for storing liquid of liquid；For the pumping installations by the liquid pumping in the device for storing liquid to the test tube bottle, the pumping installations is in fluid communication with the liquid separation needle for injecting mixed liquor into the test tube bottle；For monitoring the imaging device of chip status in the test tube bottle；The data processing equipment of the acquisition of information results of hybridization of biochip state based on imaging device acquisition.Bio-chip test device provided by the utility model realizes that biochip reaction is integrated with testing result output, improves the treatment effeciency of sample, saves manpower, as a result more stable and reliable.
Description
Technical field
The utility model relates to chip hybridization device fields, in particular to a kind of bio-chip test device.
Background technique
Biochip hybridization, which refers to, carries out albumen, DNA or the RNA sample and biochip that are separated to from biological sample instead
It answers, obtains the sequence information of sample from the probe array for being fixed on chip.Since the autofluorescent background of slide itself is very low, so can
With the method for fluorescent marker come to biochip examinations and analysis, while having many advantages, such as quick, accurate and safe.And
And multiple fluoresceins can be used to be marked also to realize and disposably analyze multiple biological samples.
Mainly there is following patent disclosed in equipment about biochip hybridization:
Disclose a kind of biochip hybridizing device application No. is 201620379820.0, feature be include hybridization wet box and
Hybridizing box, hybridize be horizontally disposed in wet box have it is multiple for putting the storing chambers of hybridizing box, hybridizing box include hybridizing box box body and
Hybridizing box box cover is provided with chip placement cavity in hybridizing box box body, and hybridizing box box cover outer end face, which is arranged side by side, multiple observations
Window, the edge of watch window are provided with a well, and hybridizing box box cover inner face is provided with for by biochip position
The position of securing chip positioning block, chip positioning block is corresponding with the position of chip placement cavity, hybridizing box box body with it is miscellaneous
One end of box box cover is handed over to be connected by hinge, hybridizing box box body is connect with the other end of hybridizing box box cover by latch components.It is excellent
Point is that structure is simple, can effectively avoid the generation of slide plate phenomenon, guarantees the success rate of hybridization, and give biological core by hybridization wet box
The hybridization of piece provides a constant hybridization space, and the ancillary equipment additional without other saves cost.
Application No. is 200710117961.0 to disclose a kind of biochip hybridization instrument, comprising: hybridization chamber, chip load plate,
Rotating part, temperature control portion and main control part；The setting of chip load plate is intracavitary in hybridization, and chip load plate passes through rotating part and drives；Rotating part is logical
It crosses main control part and carries out rotation control, hybridization chamber adjusts cavity temperature by the temperature control portion.The invention also discloses one kind
The biochip hybridization procedure optimization control method of biochip hybridization instrument, includes the following steps 1: in advance by flushing liquor as miscellaneous
It hands in chamber, then biochip cartridge to be hybridized is placed on chip load plate, the temperature for hybridizing chamber is risen to by conjunction by temperature control portion
Suitable temperature；Step 2: main control part controls the load plate whirling vibration of rotating part driving chip according to the parameter of oscillation of setting；Step 3:
After hybridization, remaining hybridization solution is dried by rotating part.The utility model realizes a kind of hybridization hybridized automatically
The hybridization control method of instrument and optimization.
Currently, also not about the record of nucleic acid amplification hybridization integral type instrument.
In view of this, special propose the utility model.
Utility model content
The purpose of this utility model is to provide a kind of bio-chip test device, realize that biochip reaction and detection are tied
Fruit output integration, improves the treatment effeciency of sample, saves manpower, as a result more stable and reliable.
In order to realize the above-mentioned purpose of the utility model, the following technical scheme is adopted:
A kind of bio-chip test device, including the cavity for carrying test tube bottle, the test tube bottle is to be checked for accommodating
Test sample sheet and biochip, the cavity have function of temperature control；
For storing the device for storing liquid of liquid；
For the pumping installations by the liquid pumping in the device for storing liquid to the test tube bottle, the pumping installations and use
It is in fluid communication in the liquid separation needle for injecting mixed liquor into the test tube bottle；
For monitoring the imaging device of chip status in the test tube bottle；
The data processing equipment of the acquisition of information results of hybridization of biochip state based on imaging device acquisition.
Further, the side hollow out of the cavity, the imaging device capture the bottle by the part of the hollow out
The information of biochip in body.
Further, the bottle body of the test tube bottle is flat structure.
Further, the bottle body is made of transparent material.
Further, the cavity mutually fits setting with the Bottle structure.
Further, the bio-chip test device further includes the bracket for being used to support the liquid separation needle, the bracket
It is arranged above the cavity and can moves up and down.
Further, the groove for accommodating the test tube bottle cap, the test tube bottle cap are provided at the top of the cavity
It is correspondingly arranged with the liquid separation needle.
Further, the drain needle for liquid in the test tube bottle to be discharged, the drain needle and drain are additionally provided with
Device is in fluid communication.
Further, the drain needle is disposed adjacent with the liquid separation needle, and the test tube bottle cap is correspondingly arranged one point
Liquid needle and a drain needle.
Further, the bottom of the cavity is provided with heating fin, and the temperature of the cavity is realized by cold and hot conduction
Control.
Further, the heating fin is thermoelectric module, and the thermoelectric module realizes it by coordination electrode
The conversion of cold and hot function.
Further, radiator, such as cooling fin and fan are also set up below the heating fin.
Further, the test tube bottle is multiple, and the cavity is divided into the space for carrying different reagent bottles.
Further, the device for storing liquid includes multiple liquid storage bottles, and the multiple liquid storage bottle is controlled different by liquid separatnig valve
The mixing of solution is flowed out.
Further, the liquid separatnig valve includes any one or more of combination of eight ways valve, four-way valve, triple valve.
Further, the cover board for fastening test tube bottle, the cover board are additionally provided between the bracket and the cavity
It is connect with plateau elastic, the liquid separation syringe needle is corresponded on the cover board and drainage syringe needle is provided with hole.
Further, the imaging device is movably arranged.
Further, in the side of the cavity hollow out, the direction parallel with the cavity is provided with track, it is described at
As device is slidably connected by sliding block and the track.
The utility model additionally provides a kind of biochip test method, using above-mentioned bio-chip test device into
Row, steps are as follows:
Biochip, sample to be detected and primer are put into the test tube bottle, the test tube bottle is fixed on the chamber
In body；
By control test tube bottle in liquid flow in and out and temperature realize the sample to be detected amplification and
Amplified production hybridizes with the biochip；
It is performed corresponding processing using different detection modes, imaging device obtains the letter for the biochip that hybridization is completed
Breath, results of hybridization obtain result after data processing equipment is handled.
Further, the amplification of the sample to be detected and amplified production carry out simultaneously with hybridizing for the biochip,
Buffer used is PCR amplification buffer.
Temperature control program can be adjusted correspondingly according to the difference of detectable substance and the difference of detection method, such as can be with
For typical PCR response procedures, or dual temperature PCR, such as 94 DEG C, 55 DEG C of dual temperature PCR, or the perseverances such as LAMP/RPA
Temperature amplification, etc..
Further, temperature control program are as follows: denaturation is annealed, and is extended, multiple circulations.
Further, temperature control program are as follows: 94 ± 1 DEG C of 10 ± 2s, 55 ± 1 DEG C of 20 ± 2s, 72 ± 2 DEG C of 20 ± 2s,
35-40 circulation.
Further, the detection mode includes chemoluminescence method, quantum dot fluorescence method and visualization method.
In actual operation, when detection, different detection modes can be used and carry out, same detection mode can also be used
It carries out.
It is such as carried out using different detection modes, adds reagent involved in corresponding detection mode every time, and using corresponding
Method show imaging, repeat, obtain the results of hybridization of the different testing sample of respective sample.
If same detection method can select different markers according to object difference to be detected, by under different condition
Reason, to obtain the results of hybridization of the different testing sample of respective sample.
The avidin solution of chemoluminescence method such as addition horseradish enzyme label, after the completion of hybridization, adds luminous substrate, highly sensitive
CCD image-forming component (model JAI CM-030-GE) is moved on guide rail immediately ahead of each chip, acquires chemiluminescence biology core
Picture.
The red quantum dot SA-QDs625 solution of quantum dot fluorescence method such as addition Ka source Avidin label, after the completion of hybridization,
Ultraviolet source excitation, filtering, highly sensitive CCD image-forming component are moved on guide rail immediately ahead of each chip, and acquisition quantum dot is glimmering
Photo-biological chip image.
The red quantum dot SA-QDs625 solution of visualization method such as addition Ka source Avidin label, after the completion of hybridization, addition
Wavelength white LED light source is opened in silver staining substrate solution, cleaning, and CCD image-forming component is moved on guide rail immediately ahead of each chip,
Acquire visible biological chip image.
Show that the substance of signal in addition to above-mentioned quantum dot, biotin, may also include fluorescence involved in the utility model
Substance, digoxin labelled probe, radioactive isotope, radiocontrast medium, paramagnetic ion fluorescent microsphere, electron dense substances,
Chemiluminescent labels, acoustic contrast agent, photosensitizer, colloidal gold or enzyme.
Wherein, fluorescent material include Alexa 350, Alexa 405, Alexa 430, Alexa 488, Alexa 555,
Alexa 647, AMCA, aminacrine, BODIPY 630/650, BODIPY 650/665, BODIPY-FL, BODIPY-R6G,
BODIPY-TMR, BODIPY-TRX, 5- carboxyl -4 ', 5 '-two chloro- 2 ', 7 '-dimethoxyfluoresceins, 5- carboxyl -2 ', 4 ',
5 ', 7 '-tetrachlorofluoresceins, 5- Fluoresceincarboxylic acid, 5- carboxyrhodamine, 6- carboxyrhodamine, 6- carboxyl tetramethylrhodamine,
Cascade Blue, Cy2, Cy3, Cy5, Cy7,6-FAM, dansyl Cl, fluorescein, HEX, 6-JOE, NBD (7- nitro benzo-
2- oxa- -1,3- diazole), Oregon Green 488, Oregon Green 500, Oregon Green514, Pacific
Blue, phthalic acid, terephthalic acid (TPA), M-phthalic acid, cresols consolidate purple, cresols royal purple, brilliant cresyl blue, p-aminophenyl first
Acid, erythrosine, phthalocyanine, azomethine, cyanine, xanthine, succinylfluoresceins, rare earth metal cryptate, three pairs of pyridines
Base diamines europium, europium cryptate or chelate, diamines, dicyanin, La Jolla indigo plant dyestuff, allophycocyanin,
Allococyanin B, phycocyanin C, phycocyanin R, thiamines, algae red green white, phycoerythrin R, REG, rhodamine be green, sieve
Red bright isothiocyanates, rhodamine be red, ROX, TAMRA, TET, TRIT (the different mercaptan of tetramethylrhodamine), tetramethylrhodamine and
Any one of texas Red.
Radioactive isotope includes110In、111In、177Lu、18F、52Fe、62Cu、64Cu、67Cu、67Ga、68Ga、86Y、90Y、89Zr、94mTc、94Tc、99mTc、120I、123I、124I、125I、131I、154-158Gd、32P、11C、13N、15O、186Re、188Re、51Mn、52mMn、55Co、72As、75Br、76Br、82MRb and83Any one of Sr.
Enzyme includes any one of horseradish peroxidase, alkaline phosphatase and glucose oxidase.
Fluorescent microsphere are as follows: polystyrene fluorescent microsphere, inside are enclosed with rare-earth fluorescent ion europium.
Compared with prior art, the utility model has the following beneficial effects:
(1) the utility model setting has the cavity of function of temperature control, so that the amplification of sample to be detected and and biochip
Hybridization integral operational, greatly save operating procedure, save the time.
(2) the utility model controls the mixing of different liquids by liquid liquid separatnig valve, is flowed into test tube bottle by liquid separation needle,
To meet the solution requirements of different times, while waste liquid is discharged by drain needle, realizes the full-automation of hybrid process.
(3) the utility model is additionally provided with imaging device, to obtain the information of the biochip in bottle body, realizes that result obtains
Manpower is saved in the automatic operation taken.
(4) bio-chip test device provided by the utility model realizes the whole process of biochip test oneself
Dynamicization operation, improves detection efficiency, and testing result is reliable and stable.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is bio-chip test device front perspective view provided by the embodiment of the utility model；
Fig. 2 is the side isometric view of bio-chip test device provided by the embodiment of the utility model；
Fig. 3 is the rear perspective view of bio-chip test device provided by the embodiment of the utility model；
Fig. 4 is the sample application array figure of each sample of biochip provided by the embodiment of the utility model；
Fig. 5 is the chemiluminescence detection result figure of biochip provided by the embodiment of the utility model；
Fig. 6 is the fluoroscopic examination result figure of biochip provided by the embodiment of the utility model；
Fig. 7 is the visual test result figure of biochip provided by the embodiment of the utility model.
Appended drawing reference:
1- cavity；2- liquid storage bottle；3- pumping installations；4- bracket；5- imaging device；6- liquid separatnig valve.
Specific embodiment
The technical solution of the utility model is clearly and completely retouched below in conjunction with the drawings and specific embodiments
It states, it will be appreciated by those skilled in the art that following, the embodiments are a part of the embodiments of the present invention, without
It is whole embodiments, is merely to illustrate the utility model, and is not construed as limitation the scope of the utility model.It is practical based on this
Embodiment in novel, every other reality obtained by those of ordinary skill in the art without making creative efforts
Example is applied, is fallen within the protection scope of the utility model.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer
It is recommended that condition carry out.Reagents or instruments used without specified manufacturer is the routine that can be obtained by commercially available purchase
Product.
It is in the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", " perpendicular
Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only
For ease of description the utility model and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific
Orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.In addition, term " the
One ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition
The concrete meaning of language in the present invention.
As shown in Figure 1-3, bio-chip test device provided by the utility model, including the cavity for carrying test tube bottle
1, the test tube bottle has function of temperature control for accommodating sample and biochip to be detected, the cavity 1；
For storing the device for storing liquid of liquid；
For the pumping installations 3 by the liquid pumping in the device for storing liquid to the test tube bottle, the pumping installations 3 with
Liquid separation needle for injecting from mixed liquor to the test tube bottle is in fluid communication；
For monitoring the imaging device 5 of chip status in the test tube bottle；
The data processing equipment of the acquisition of information results of hybridization of biochip state based on the imaging device 5 acquisition.
Existing biochip hybridization and results of hybridization detection process are as follows:
Preparation has the biochip of different probe: different probe buffers point on substrate with gene chip sample applying instrument
Sample is dried for standby；
Hybridization is prepared with PCR product: being treated detectable substance and is carried out PCR amplification；
Biochip and PCR product are hybridized: pcr amplification product is denaturalized (98 DEG C), cryostat immediately, then slow with hybridization
Fliud flushing mixing, the mixed liquor are added to the point sample area of chip, chip are put into hybridizing box, and hybridization is protected from light in baking oven；Through keeping away
After light washing, it to be used for scanning analysis.
Bio-chip test device provided by the utility model, sample and biochip to be detected are directly added into test tube bottle
In, it is flowed in and out by control temperature and corresponding solution, sample to be detected is miscellaneous with biochip while PCR is expanded
It hands over, then passes through after corresponding washing lotion is added, handled using different detection modes, imaging device 5 obtains hybridization and completes
Biochip information, results of hybridization obtains result after data processing equipment is handled.
It should be noted that the biochip of the utility model both includes genetic chip, it also include protein chip, i.e. this reality
It both can be used for genetic test with the bio-chip test device of novel offer, can be used for albumen such as immune detection etc..
The utility model realizes the automatic operation of the whole process of biochip test, improves detection efficiency, detection
As a result reliable and stable.
In the utility model, for carrying test tube bottle, cavity 1 can be surrounded by side wall cavity 1 completely, can also be frame
Frame is constituted.Based on the progress convenient for camera shooting and it is convenient for temperature control, in some embodiments, the side hollow out (Fig. 3) of the cavity 1,
The imaging device 5 captures the information of the biochip in the bottle body by the part of the hollow out.
Further, the part setting of the camera face hollow out of imaging device 5, in order to the intake of image.
In some embodiments, the bottle body of the test tube bottle is flat structure.
For bottle body for containing biochip and sample to be detected, biochip is generally the sheet of quadrilateral, and to be detected
Sample is liquid, therefore, the shape and biochip similar set up of bottle body；The space of bottle body is arranged slightly larger than biochip, with
It is readily integrated into convenient for biochip, is also convenient for the information that imaging device 5 absorbs biochip in this way.
In some embodiments, the bottle body is made of transparent material.In order to which imaging device 5 absorbs the letter of biochip
Breath.
In some embodiments, the cavity 1 mutually fits setting with the Bottle structure.
Since cavity 1 has temperature control, and the temperature of test tube bottle is usually to be controlled by 1 heat transfer of cavity, therefore, cavity 1
Setting is mutually fitted with the Bottle structure.
In some embodiments, the bio-chip test device further includes the bracket 4 for being used to support the liquid separation needle, institute
Bracket 4 is stated to be arranged above the cavity 1 and can move up and down.
The top of reagent bottle is arranged in liquid separation needle, is located on bracket 4, by the movement of bracket 4 realize the insertion of liquid separation needle or
It extracts in the test tube bottle.
In some embodiments, the top of the cavity 1 is provided with the groove for accommodating the test tube bottle cap, the test tube
Bottle cap is correspondingly arranged with the liquid separation needle.
By the way that groove is arranged, enhances the stability of bottle cap, inserted or pull out in order to control liquid separation needle.
Different phase in hybrid process needs different solution to handle, and by the inflow and discharge of liquid, realization is changed
The automatic operation of liquid.
In some embodiments, the drain needle for liquid in the test tube bottle to be discharged, the drain needle are additionally provided with
It is in fluid communication with pumping equipment.Liquid is expelled in pumping equipment such as waste liquid bottle by drain needle.
In some embodiments, the drain needle is disposed adjacent with the liquid separation needle, and the test tube bottle cap is correspondingly arranged
One liquid separation needle and a drain needle.In i.e. each test tube bottle, the inflow of liquid is controlled by liquid separation needle and drain needle controls liquid
The automatic operation for changing liquid is realized in the discharge of body.
In some embodiments, the bottom of the cavity 1 is provided with heating fin, realizes the chamber by cold and hot conduction
The temperature control of body 1.
In some embodiments, the heating fin is thermoelectric module, and the thermoelectric module passes through coordination electrode
Realize the conversion of its cold and hot function.
In some embodiments, radiator, such as cooling fin and fan are also set up below the heating fin.
The temperature-controllable in test tube bottle is realized by heating device and radiator.
In some embodiments, the test tube bottle is multiple, and the cavity 1 divides for the sky for carrying different reagent bottles
Between.Generally, multiple independent spaces are arranged in cavity 1, and each space is for placing a reagent bottle, the size and examination in the space
Agent bottle size mutually fits setting, with the stability of the placement of Contrast agent bottle.
In some embodiments, the device for storing liquid includes multiple liquid storage bottles 2, and the multiple liquid storage bottle 2 passes through liquid separatnig valve
The mixing outflow of 6 control different solutions.
In some embodiments, the liquid separatnig valve 6 is any one or more of including eight ways valve, four-way valve, triple valve
Combination.
In some embodiments, the cover board for fastening test tube bottle is additionally provided between the bracket 4 and the cavity 1,
The cover board is connect with plateau elastic, and the liquid separation syringe needle is corresponded on the cover board and drainage syringe needle is provided with hole.
By the way that cover board is arranged, so that the placement that test tube bottle is relatively fixed.
In some embodiments, the imaging device 5 is movably arranged.
In some embodiments, in the side of 1 hollow out of cavity, rail is set along the direction parallel with the cavity 1
Road, the imaging device 5 are slidably connected by sliding block and the track.
In actual operation, according to the test tube bottle of different location, the position of imaging device 5 is controlled, to realize to different examinations
The acquisition of biochip information in phial.
Bio-chip test device provided by the utility model, flowing, movement of bracket 4 of liquid etc. pass through control system
It completes, control system and data processing system are arranged in the same operation pages.
Control system had both included pcb board etc., as being equipped with micro-chip processor, temperature control module, drive module, touching on pcb board
Liquid crystal screen module, USB module, JTAG module, alarm module etc. are touched, the micro-chip processor is connected with modules respectively,
And modules are controlled, realize the accurate progress of whole operation.
The utility model additionally provides a kind of biochip test method, using above-mentioned bio-chip test device into
Row, steps are as follows:
Biochip, sample to be detected and primer are put into the test tube bottle, the test tube bottle is fixed on the chamber
In body 1；
By control test tube bottle in liquid flow in and out and temperature realize the sample to be detected amplification and
Amplified production hybridizes with the biochip；
It is performed corresponding processing using different detection modes, imaging device 5 obtains the letter for the biochip that hybridization is completed
Breath, results of hybridization obtain result after data processing equipment is handled.
It should be noted that bio-chip test device provided by the utility model is not install showing for various pipelines etc.
It is intended to, in use, needing to be installed accordingly, just can be used.
It is specific to carry out following citing:
Swin flu, the second stream, RSV, adenovirus of sample are detected, designs primer and the spy of these types of detectable substance according to a conventional method
Needle, it is specific as shown in table 1.
1 particular sequence of table
Nucleic acid probe point sample: biochip is self-produced aldehyde radical slide, and probe spotting concentration is 10 μM, and drying at room temperature is protected
It deposits, schematic diagram is as shown in Figure 4.
PCR amplification: sample of nucleic acid (object to be detected), biochip and PCR mix reagent (including primer and buffer) are mixed
It closes, is added in instrument test tube bottle, instrument semiconductor heating refrigerating plate thermal circulation parameters: 94 DEG C of 10s, 55 DEG C of 20s, 72 DEG C of 20s, 40
A circulation is realized and becomes the hybridization of amplification side.Then washing lotion is pumped by syringe pump and solenoid valve and cleans chip.
Subsequent 3 kinds of instruments and reagent of being respectively adopted realize chemiluminescence, 3 kinds of detection sides of quantum dot fluorescence and visualization
Formula.
Chemoluminescence method detection: it is pumped into the avidin solution that 100 μ L horseradish enzymes mark, 37 DEG C of reactions automatically to each reaction flask
30min.It is pumped into the cleaning of PBST washing lotion.Be pumped into again 100 μ L Chemoluminescent substrates (Mi Libogaomin Chemoluminescent substrate A liquid:
B liquid=1:1).Highly sensitive CCD image-forming component (model JAI CM-030-GE) is moved on guide rail immediately ahead of each chip, is adopted
Collect chemiluminescence biochip image, every chip time for exposure 10s amounts to 8 chips.As a result as shown in Figure 5.This image knot
Fruit shows that the sample is that Flu-A nucleic acid is positive.
The detection of quantum dot fluorescence method: it is pumped into the red that 100 μ L, 25nM Wuhan Ka source Avidins mark automatically to each reaction flask
Quantum dot SA-QDs625 solution, 37 DEG C of reaction 30min.It is pumped into the cleaning of PBST washing lotion.Open wavelength 365nm, 5W ultraviolet LED light
Source excitation QDs emits fluorescence, and filters through optical filter (624/40nm, model Edmund Optical 67-021), highly sensitive
CCD image-forming component is moved on guide rail immediately ahead of each chip, acquires quantum dot fluorescent biochip image, and every chip exposes
10s between light time amounts to 8 chips.As a result as shown in Figure 6.This image result shows that the sample is that Flu-A nucleic acid is positive.
Visual retrieval: it is pumped into the red quantum dot that 100 μ L, 25nM Wuhan Ka source Avidins mark automatically to each reaction flask
SA-QDs625 solution, 37 DEG C of reaction 30min.It is pumped into the cleaning of PBST washing lotion.It is pumped into 100 μ L silver staining substrate solutions again, reacts 5min,
Deionized water cleaning.Wavelength white light LEDs light source is opened, CCD image-forming component is moved to immediately ahead of each chip on guide rail, adopted
Collect visible biological chip image, amounts to 8 chips.As a result as shown in Figure 7.This image result shows that the sample is Flu-A
Nucleic acid is positive.
Bio-chip test device provided by the utility model, sample and biochip to be detected are directly added into test tube bottle
In, it is flowed in and out by control temperature and corresponding solution, the reaction product of sample PCR amplification is while amplification and biological
Then chip detection passes through after corresponding washing lotion is added, images to chip row, obtains information, final results of hybridization can be obtained.
It realizes that the hybrid process of genetic chip is integrated with result output, improves the treatment effeciency of sample, save manpower, it is as a result more stable
Reliably.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited
System；Although the present invention has been described in detail with reference to the aforementioned embodiments, but those skilled in the art answer
Work as understanding: it is still possible to modify the technical solutions described in the foregoing embodiments, either to part of or complete
Portion's technical characteristic is equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical
The range of novel each embodiment technical solution.
Claims (10)
1. a kind of bio-chip test device, which is characterized in that including the cavity for carrying test tube bottle, the test tube bottle is used for
Sample and biochip to be detected are accommodated, the cavity has function of temperature control；
For storing the device for storing liquid of liquid；
For the pumping installations by the liquid pumping in the device for storing liquid to the test tube bottle, the pumping installations with for
The liquid separation needle that mixed liquor is injected in the test tube bottle is in fluid communication；
For monitoring the imaging device of chip status in the test tube bottle；
The data processing equipment of the acquisition of information results of hybridization of biochip state based on imaging device acquisition.
2. bio-chip test device according to claim 1, which is characterized in that the side hollow out of the cavity, it is described
Imaging device captures the information of the biochip in the bottle body by the part of the hollow out.
3. bio-chip test device according to claim 1, which is characterized in that the bottle body of the test tube bottle is flat knot
Structure, the cavity mutually fit setting with the Bottle structure.
4. bio-chip test device according to claim 1, which is characterized in that further include being used to support the liquid separation needle
Bracket, the bracket is arranged above the cavity and can move up and down.
5. bio-chip test device according to claim 4, which is characterized in that between the bracket and the cavity also
It is provided with the cover board for fastening test tube bottle, the cover board is connect with plateau elastic, and the liquid separation syringe needle is corresponded on the cover board
Drainage syringe needle is provided with hole.
6. bio-chip test device according to claim 1, which is characterized in that be provided with receiving at the top of the cavity
The groove of the test tube bottle cap, the test tube bottle cap are correspondingly arranged with the liquid separation needle；
It is additionally provided with the drain needle for liquid in the test tube bottle to be discharged, the drain needle and pumping equipment are in fluid communication；
The drain needle is disposed adjacent with the liquid separation needle, and the test tube bottle cap is correspondingly arranged a liquid separation needle and a drain
Needle.
7. bio-chip test device according to claim 1, which is characterized in that the bottom of the cavity is provided with heating
Cooling piece realizes the temperature control of the cavity by cold and hot conduction.
8. bio-chip test device according to claim 1, which is characterized in that the test tube bottle is multiple, the chamber
Body is divided into the space for carrying different reagent bottles；
The device for storing liquid includes multiple liquid storage bottles, and the multiple liquid storage bottle controls the mixed flow of different solutions by liquid separatnig valve
Out.
9. bio-chip test device according to claim 1-8, which is characterized in that the imaging device is removable
Dynamic setting.
10. bio-chip test device according to claim 9, which is characterized in that in the side of the cavity hollow out, edge
The direction parallel with the cavity is provided with track, and the imaging device is slidably connected by sliding block and the track.
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| CN201821555153.2U CN209039485U (en) | 2018-09-21 | 2018-09-21 | A kind of bio-chip test device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055203A (en) * | 2018-09-21 | 2018-12-21 | 中国人民解放军军事科学院军事医学研究院 | A kind of full-automatic biological chips work station and its detection method |
| CN113755647A (en) * | 2021-10-15 | 2021-12-07 | 无锡市人民医院 | Novel coronavirus rapid detection system |
-
2018
- 2018-09-21 CN CN201821555153.2U patent/CN209039485U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055203A (en) * | 2018-09-21 | 2018-12-21 | 中国人民解放军军事科学院军事医学研究院 | A kind of full-automatic biological chips work station and its detection method |
| CN113755647A (en) * | 2021-10-15 | 2021-12-07 | 无锡市人民医院 | Novel coronavirus rapid detection system |
| CN113755647B (en) * | 2021-10-15 | 2023-08-22 | 无锡市人民医院 | Novel coronavirus rapid detection system |
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Inventor after: Wang Shengqi Inventor after: Rong Zhen Inventor after: Xiao Rui Inventor before: Wang Shengqi Inventor before: Rong Zhen Inventor before: Xiao Rui Inventor before: Wang Feng |