AR124664A1 - FORK-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN A SOLID PHASE MEDIUM - Google Patents

FORK-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN A SOLID PHASE MEDIUM

Info

Publication number
AR124664A1
AR124664A1 ARP220100088A ARP220100088A AR124664A1 AR 124664 A1 AR124664 A1 AR 124664A1 AR P220100088 A ARP220100088 A AR P220100088A AR P220100088 A ARP220100088 A AR P220100088A AR 124664 A1 AR124664 A1 AR 124664A1
Authority
AR
Argentina
Prior art keywords
lamp
solid phase
present disclosure
reaction
layer disposed
Prior art date
Application number
ARP220100088A
Other languages
Spanish (es)
Inventor
Aaron Adler
Bryan Bartley
Mike Gavin
Jordan Seville
Darby Mchesney
Frank M Lduca
Osman Fujr Osman Ibrahim
Mohit Verma
Original Assignee
Purdue Research Foundation
Raytheon Bbn Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation, Raytheon Bbn Technologies Corp filed Critical Purdue Research Foundation
Publication of AR124664A1 publication Critical patent/AR124664A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente divulgación se refiere a conjuntos de reacción de amplificación isotérmica mediada por horquillas (LAMP) que incluyen una mezcla de reactivos LAMP sustancialmente libre de agentes higroscópicos en combinación con un medio de reacción en fase sólida. La presente divulgación también incluye sistemas para un análisis LAMP cromático que incluyen un medio de reacción en fase sólida sustancialmente no reactivo y una mezcla de reactivos no interferentes. La presente divulgación también incluye medios de reacción LAMP en fase sólida que comprenden un sustrato, una capa adhesiva dispuesta sobre el sustrato, una capa de reacción dispuesta sobre la capa adhesiva, y una capa de extensión dispuesta sobre la capa de reacción. La presente divulgación también incluye métodos de ensayo para detectar la presencia de una secuencia de nucleótidos objetivo que incluyen proporcionar una muestra biológica, y dispensar la muestra en un entorno de prueba que tiene un medio de reacción en fase sólida en combinación con una mezcla de reactivos LAMP y un colorante sensible al pH.The present disclosure relates to hairpin-mediated isothermal amplification (LAMP) reaction sets that include a LAMP reagent mixture substantially free of hygroscopic agents in combination with a solid phase reaction medium. The present disclosure also includes systems for chromatic LAMP analysis that include a substantially non-reactive solid phase reaction medium and a mixture of non-interfering reagents. The present disclosure also includes solid phase LAMP reaction media comprising a substrate, an adhesive layer disposed on the substrate, a reaction layer disposed on the adhesive layer, and an extension layer disposed on the reaction layer. The present disclosure also includes assay methods for detecting the presence of a target nucleotide sequence including providing a biological sample, and dispensing the sample in a test environment having a solid phase reaction medium in combination with a mixture of reagents. LAMP and a pH sensitive stain.

ARP220100088A 2021-01-15 2022-01-17 FORK-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN A SOLID PHASE MEDIUM AR124664A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163138321P 2021-01-15 2021-01-15

Publications (1)

Publication Number Publication Date
AR124664A1 true AR124664A1 (en) 2023-04-19

Family

ID=81025318

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220100088A AR124664A1 (en) 2021-01-15 2022-01-17 FORK-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN A SOLID PHASE MEDIUM

Country Status (4)

Country Link
EP (1) EP4278003A1 (en)
AR (1) AR124664A1 (en)
TW (1) TW202246524A (en)
WO (1) WO2022155550A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11123736B2 (en) * 2016-03-14 2021-09-21 Lucira Health, Inc. Systems and methods for performing biological assays
JP2022538046A (en) * 2019-06-18 2022-08-31 マンモス バイオサイエンシズ インコーポレイテッド Assays and methods for detecting nucleic acids
WO2021183921A1 (en) * 2020-03-12 2021-09-16 New England Biolabs, Inc. A rapid diagnostic test for lamp

Also Published As

Publication number Publication date
WO2022155550A1 (en) 2022-07-21
EP4278003A1 (en) 2023-11-22
TW202246524A (en) 2022-12-01

Similar Documents

Publication Publication Date Title
BR112022019754A2 (en) SARS-COV-2 DETECTION TESTS
US11761955B2 (en) Multiplexed catalyzed reporter deposition
Seok et al. A paper-based device for performing loop-mediated isothermal amplification with real-time simultaneous detection of multiple DNA targets
US20200096502A1 (en) Analyte Detection
BRPI0510680B8 (en) device and method for qualitative and/or quantitative detection of molecular interactions between probe molecules and target molecules, and, use of a device
TW200801203A (en) Multiplexed analyses of test samples
HK1150171A1 (en) Compositions and methods for analysis of nucleic acid molecules during amplification reactions
WO2011069029A3 (en) Lateral flow assays
ATE527383T1 (en) MULTIPLEX NUCLEIC ACID DETECTION
AR053251A1 (en) NEW LABELING STRATEGIES FOR THE SENSITIVE DETECTION OF ANALYTES
TW200613564A (en) Bacteria detection instrument, bacteria detection method and bacteria detection kit
WO2019038706A3 (en) Paper-based molecular diagnostic device and uses thereof
Terai et al. A long-lived luminescent probe to sensitively detect arylamine N-acetyltransferase (NAT) activity of cells
US9835623B2 (en) Method and system of microfluidic immunoassay using magnetic beads
Pereira et al. Miniaturized technologies for high-throughput drug screening enzymatic assays and diagnostics–A review
HK1130292A1 (en) Dry format sialidase assay
BR112022019764A2 (en) SARS-COV-2 DETECTION TESTS
WO2006103450A3 (en) Electrochemical assay
AR124664A1 (en) FORK-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN A SOLID PHASE MEDIUM
AR124658A1 (en) FORK-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN A SOLID PHASE MEDIUM
AR124663A1 (en) FORK-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN A SOLID PHASE MEDIUM
Kricka Chemiluminescence
JP2018538514A (en) Decoding method for multiplexing assays and related fluidic devices, kits, and solid supports
CL2023002060A1 (en) Loop-mediated isothermal amplification (lamp) on a solid phase medium
BR112013027041A2 (en) Method of detection of an analyte and kit

Legal Events

Date Code Title Description
FB Suspension of granting procedure