AR120629A1 - Kit de diagnóstico de fundamento molecular para la detección de secuencias nucleotídicas, y métodos para detectar agentes infecciosos usando dicho kit - Google Patents

Kit de diagnóstico de fundamento molecular para la detección de secuencias nucleotídicas, y métodos para detectar agentes infecciosos usando dicho kit

Info

Publication number
AR120629A1
AR120629A1 ARP190102073A ARP190102073A AR120629A1 AR 120629 A1 AR120629 A1 AR 120629A1 AR P190102073 A ARP190102073 A AR P190102073A AR P190102073 A ARP190102073 A AR P190102073A AR 120629 A1 AR120629 A1 AR 120629A1
Authority
AR
Argentina
Prior art keywords
reaction
dropper
sample
support
infectious agents
Prior art date
Application number
ARP190102073A
Other languages
English (en)
Inventor
Fabiana Stolowicz
Luciana Larocca
Adrian Vojnov
Carolina Carrillo
Original Assignee
Laboratorio Pablo Cassara S R L
Unifarma S A
Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Laboratorio Pablo Cassara S R L, Unifarma S A, Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet filed Critical Laboratorio Pablo Cassara S R L
Priority to ARP190102073A priority Critical patent/AR120629A1/es
Priority to PCT/CL2020/050079 priority patent/WO2021012063A1/es
Priority to EP20843787.1A priority patent/EP4008791A4/en
Priority to MX2022000916A priority patent/MX2022000916A/es
Priority to US17/597,777 priority patent/US20230064848A1/en
Priority to BR112022001206A priority patent/BR112022001206A2/pt
Publication of AR120629A1 publication Critical patent/AR120629A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Un kit de diagnóstico de fundamento molecular para la detección de secuencias nucleotídicas de ADN, ADN copia y ARN, provenientes de agentes infecciosos, transgenes, alelos o secuencias no codificantes, sin necesidad de realizar pasos de aislamiento inicial, que comprende: al menos un primer frasco gotero calibrado con sistema anticontaminación, conteniendo una solución de la enzima polimerasa, agentes caotrópicos, sales y desoxitibonucleótidos; un segundo frasco gotero calibrado con sistema anticontaminación, conteniendo un set de al menos 4 primers, con al menos parte de sus secuencias conservadas en el fragmento de ácido nucleico que desea detectarse; un gotero con la mezcla de reactivos de revelado; un soporte para colocar la muestra a evaluar; al menos un sistema de revelado (por LFD o por reacción colorimétrica o fluorogénica); controles positivos y negativos; donde la enzima polimerasa se selecciona de la familia de enzimas BST, que está formada por DNA polimerasas / helicasas, sin actividad exonucleasa; y el set de primers se diseña a partir de la secuencias génica que desea detectarse, ya sea de naturaleza desoxirribonucleica o ribonucleica. El soporte puede ser un tubo o una bandeja de reacción o bien un papel de filtro o similar impregnado con la muestra, colocado en uno de dichos continentes. El método para detectar agentes infecciosos comprende los pasos de: colocar la muestra dentro del soporte, colocar al menos una gota del primer gotero dentro del continente de reacción, colocar al menos una gota del segundo gotero dentro del continente de reacción, tapar el continente, mezclar hasta homogeneizar los componentes de la solución, dar lugar a la reacción isotérmica de amplificación, mezclar y leer el resultado por cambio de color o fluorescencia. Si el método de lectura es por cromatografía en tiras reactivas, adicionar a la reacción una gota del gotero del revelado, introducir una tira reactiva LFD dentro del tubo, dejar que el contenido de la reacción de amplificación avance por capilaridad a lo largo de la LFD por unos 2 - 5 minutos, y leer el resultado. Si el soporte es un papel de filtro la parte con la muestra se coloca dentro del tubo de reacción o la bandeja de reacción.
ARP190102073A 2019-07-23 2019-07-23 Kit de diagnóstico de fundamento molecular para la detección de secuencias nucleotídicas, y métodos para detectar agentes infecciosos usando dicho kit AR120629A1 (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ARP190102073A AR120629A1 (es) 2019-07-23 2019-07-23 Kit de diagnóstico de fundamento molecular para la detección de secuencias nucleotídicas, y métodos para detectar agentes infecciosos usando dicho kit
PCT/CL2020/050079 WO2021012063A1 (es) 2019-07-23 2020-07-22 Kit de diagnóstico de fundamento molecular para la deteccion de secuencias nucleotídicas y métodos para detectar agentes infecciosos usando dicho kit
EP20843787.1A EP4008791A4 (en) 2019-07-23 2020-07-22 MOLECULAR DIAGNOSTIC KIT FOR DETECTING NUCLEOTIDE SEQUENCES AND METHOD FOR DETECTING INFECTIOUS GERMS USING THIS KIT
MX2022000916A MX2022000916A (es) 2019-07-23 2020-07-22 Kit de diagnostico de fundamento molecular para la deteccion de secuencias nucleotidicas y metodos para detectar agentes infecciosos usando dicho kit.
US17/597,777 US20230064848A1 (en) 2019-07-23 2020-07-22 Molecular diagnostic kit for detecting nucleotide sequences and methods for detecting infectious agents using said kit
BR112022001206A BR112022001206A2 (pt) 2019-07-23 2020-07-22 Kit de diagnóstico de fundamento molecular para a detecção de sequências de nucleotídeos e métodos para detectar agentes infecciosos através do seu uso

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP190102073A AR120629A1 (es) 2019-07-23 2019-07-23 Kit de diagnóstico de fundamento molecular para la detección de secuencias nucleotídicas, y métodos para detectar agentes infecciosos usando dicho kit

Publications (1)

Publication Number Publication Date
AR120629A1 true AR120629A1 (es) 2022-03-02

Family

ID=74192566

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP190102073A AR120629A1 (es) 2019-07-23 2019-07-23 Kit de diagnóstico de fundamento molecular para la detección de secuencias nucleotídicas, y métodos para detectar agentes infecciosos usando dicho kit

Country Status (6)

Country Link
US (1) US20230064848A1 (es)
EP (1) EP4008791A4 (es)
AR (1) AR120629A1 (es)
BR (1) BR112022001206A2 (es)
MX (1) MX2022000916A (es)
WO (1) WO2021012063A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112795475A (zh) * 2021-03-26 2021-05-14 上海兰卫医学检验所股份有限公司 适用于非人源样品的新冠病毒检测系统及其应用
CN114457195A (zh) * 2022-02-21 2022-05-10 中国科学院地球化学研究所 基于lamp和crispr的病毒检测试剂盒和方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997006439A1 (en) 1995-08-09 1997-02-20 Quidel Corporation Test strip and method for one step lateral flow assay
US5830714A (en) 1996-04-17 1998-11-03 Molecular Biology Resources, Inc. Biologically active fragment of bacillus stearothermophilus DNA polymerase
ES2393056T3 (es) 1998-11-09 2012-12-18 Eiken Kagaku Kabushiki Kaisha Procedimiento de síntesis de ácido nucleico
WO2011087782A2 (en) * 2009-12-22 2011-07-21 The Administrators Of The Tulane Educational Fund Compositions, kits and methods for detection of trypanosoma cruzi using loop-mediated isothermal amplication
US20130210016A1 (en) * 2012-02-15 2013-08-15 Lawrence Livermore National Security, Llc Nucleic acid detection and related compositions methods and systems
CN102925548B (zh) 2012-08-02 2014-12-24 四川农业大学 猪传染性胸膜肺炎放线杆菌lamp试剂盒及使用方法
US8993298B1 (en) 2012-08-31 2015-03-31 New England Biolabs, Inc. DNA polymerases
CN103409555B (zh) * 2013-07-19 2015-01-07 浙江省淡水水产研究所 罗氏沼虾野田村病毒rt-lamp-lfd检测方法及其检测试剂盒
CN104342497A (zh) * 2014-11-25 2015-02-11 浙江省医学科学院 缺失RoTat1.2基因伊氏锥虫变异株的LAMP检测方法
US10072309B1 (en) 2015-05-08 2018-09-11 Dougbeh-Chris Nyan Methods for real-time multiplex isothermal detection and identification of bacterial, viral, and protozoan nucleic acids
KR102523355B1 (ko) * 2016-12-23 2023-04-20 알베르트-루드빅스-유니베르지텟 푸라이부르그 2-조각 매개체 프로브
CN111378784A (zh) * 2020-03-06 2020-07-07 齐鲁工业大学 新型冠状病毒SARS-CoV-2核酸目视检测试剂盒

Also Published As

Publication number Publication date
EP4008791A1 (en) 2022-06-08
BR112022001206A2 (pt) 2022-06-07
WO2021012063A4 (es) 2021-02-25
MX2022000916A (es) 2022-07-12
WO2021012063A1 (es) 2021-01-28
EP4008791A4 (en) 2024-01-17
US20230064848A1 (en) 2023-03-02

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