AR098080A1 - Secuencias de glifosato-n-acetiltransferasa (glyat) y métodos de uso - Google Patents

Secuencias de glifosato-n-acetiltransferasa (glyat) y métodos de uso

Info

Publication number
AR098080A1
AR098080A1 ARP140103885A ARP140103885A AR098080A1 AR 098080 A1 AR098080 A1 AR 098080A1 AR P140103885 A ARP140103885 A AR P140103885A AR P140103885 A ARP140103885 A AR P140103885A AR 098080 A1 AR098080 A1 AR 098080A1
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AR
Argentina
Prior art keywords
polypeptide
plant
cell
explant
seed
Prior art date
Application number
ARP140103885A
Other languages
English (en)
Inventor
E Mbride Kevin
Bermudez Ericka
A Castle Linda
Siehl Daniel
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of AR098080A1 publication Critical patent/AR098080A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Reivindicación 1: Un polinucleótido aislado o recombinante que comprende una secuencia de nucleótidos que codifican un polipéptido, en donde el polipéptido tiene actividad glifosato-N-acetiltransferasa (GLYAT) y comprende una secuencia de aminoácido que comprende la sec. con núm. de ident.: 174. Reivindicación 2: El polinucleótido aislado o recombinante de la reivindicación 1, en donde el polinucleótido codifica un polipéptido que comprende una secuencia de aminoácidos que tiene al menos 60%, 70%, 80%, 90% ó 95% de identidad de secuencia a través de la longitud total de cualquiera de las sec. con núms. de ident.: 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, ó 172. Reivindicación 8: Un constructo de ácido nucleico que comprende el polinucleótido aislado o recombinante de cualquier reivindicación 1 - 7. Reivindicación 15: La célula de la reivindicación 14, en donde la monocotiledónea es maíz, trigo, arroz, cebada, caña de azúcar, sorgo, césped, o centeno. Reivindicación 21: La planta, el explante, o la célula vegetal de la reivindicación 20, en donde el al menos un polipéptido que imparte tolerancia a un herbicida adicional comprende: (a) una acetolactato sintasa tolerante a sulfonilurea; (b) una acetolactato sintasa tolerante a imidazolinona; (c) una 4-hidroxifenilpiruvato dioxigenasa; (d) una fosfinotricina acetil transferasa; (e) una protoporfirinogeno oxidasa; (f) un gen de tolerancia a auxina; (g) un polipéptido P450; (h) una acetil coenzima A carboxilasa (ACCasa); (i) una dicamba monooxigenasa; o (j) una ariloxialcanoato dioxigenasa. Reivindicación 37: Un método para la detección de un polipéptido glifosato-N-acetiltransferasa; el método comprende analizar los tejidos de las plantas mediante el uso de un inmunoensayo que comprende un anticuerpo o anticuerpos que reconocen específicamente un polipéptido de cualquier reivindicación 26 - 32, en donde el anticuerpo o anticuerpos se levantan contra un polipéptido de cualquier reivindicación 26 - 32 como un antígeno. Reivindicación 40: Un método para obtener un alimento o producto alimenticio a partir de una planta, explante, célula vegetal, célula, o semilla de cualquier reivindicación 12 - 25; el método comprende: a) obtener una planta, explante, célula, célula vegetal o semilla de cualquier reivindicación 12 - 25; y b) procesar la planta, explante, célula, célula vegetal o semilla para producir el alimento o el producto alimenticio.
ARP140103885A 2013-10-18 2014-10-17 Secuencias de glifosato-n-acetiltransferasa (glyat) y métodos de uso AR098080A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361892663P 2013-10-18 2013-10-18

Publications (1)

Publication Number Publication Date
AR098080A1 true AR098080A1 (es) 2016-04-27

Family

ID=51794998

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP140103885A AR098080A1 (es) 2013-10-18 2014-10-17 Secuencias de glifosato-n-acetiltransferasa (glyat) y métodos de uso

Country Status (7)

Country Link
US (2) US10329578B2 (es)
CN (1) CN105874062B (es)
AR (1) AR098080A1 (es)
AU (1) AU2014334590A1 (es)
BR (1) BR112016008489A2 (es)
CA (1) CA2927180A1 (es)
WO (1) WO2015057600A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017059045A1 (en) * 2015-09-30 2017-04-06 Pioneer Hi-Bred International, Inc. Plant epsp synthases and methods of use
CN105505956B (zh) * 2016-02-02 2017-09-08 中国农业科学院北京畜牧兽医研究所 一种紫花苜蓿对羟基苯丙酮酸双加氧酶基因的应用
EP3629743B1 (en) * 2017-06-02 2023-08-16 Cytozyme Laboratories, Inc. Plant treatment products and related methods
CN107512968A (zh) * 2017-10-20 2017-12-26 桐梓县小水芦柑专业合作社 一种用于水仙花的专用营养肥料

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US20160257971A1 (en) 2016-09-08
US10329578B2 (en) 2019-06-25
CN105874062A (zh) 2016-08-17
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BR112016008489A2 (pt) 2017-10-03
CA2927180A1 (en) 2015-04-23

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