AR091868A1 - Desarrollo de los parametros del ensayo de cigosidad para detectar el gen rf4 en maiz - Google Patents

Desarrollo de los parametros del ensayo de cigosidad para detectar el gen rf4 en maiz

Info

Publication number
AR091868A1
AR091868A1 ARP130102610A ARP130102610A AR091868A1 AR 091868 A1 AR091868 A1 AR 091868A1 AR P130102610 A ARP130102610 A AR P130102610A AR P130102610 A ARP130102610 A AR P130102610A AR 091868 A1 AR091868 A1 AR 091868A1
Authority
AR
Argentina
Prior art keywords
corn
gene
cigosity
detect
development
Prior art date
Application number
ARP130102610A
Other languages
English (en)
Inventor
Ren Ruihua
Zheng Peizhong
Prasad Kumpatla Siva
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR091868A1 publication Critical patent/AR091868A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proporciona un método para determinar la expresión genética o cigosidad del gen Rf4 en una planta de cereal (maíz). Un método puede incluir llevar a cabo un primer ensayo PCR (Reacción en Cadena de la Polimerasa), un segundo ensayo PCR, calculando las sondas de muestreo utilizadas en el primer y en el segundo ensayo PCR, y comparando las sondas calculadas para determinar la cigosidad.
ARP130102610A 2012-07-23 2013-07-23 Desarrollo de los parametros del ensayo de cigosidad para detectar el gen rf4 en maiz AR091868A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261674556P 2012-07-23 2012-07-23

Publications (1)

Publication Number Publication Date
AR091868A1 true AR091868A1 (es) 2015-03-04

Family

ID=48917713

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130102610A AR091868A1 (es) 2012-07-23 2013-07-23 Desarrollo de los parametros del ensayo de cigosidad para detectar el gen rf4 en maiz

Country Status (7)

Country Link
US (1) US10214783B2 (es)
EP (1) EP2875155B1 (es)
AR (1) AR091868A1 (es)
BR (1) BR112015001587B8 (es)
CA (1) CA2879860C (es)
MX (1) MX363287B (es)
WO (1) WO2014018501A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9036391B2 (en) 2012-03-06 2015-05-19 Micron Technology, Inc. Arrays of vertically-oriented transistors, memory arrays including vertically-oriented transistors, and memory cells
US20170289805A1 (en) * 2016-03-30 2017-10-05 Motorola Mobility Llc Embedded active matrix organic light emitting diode (amoled) fingerprint sensor and self-compensating amoled
EP3379253A1 (en) * 2017-03-24 2018-09-26 Biogemma Method for determining the level of zygosity of a seed

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US20110151441A1 (en) 2009-12-18 2011-06-23 Dow Agrosciences Llc Endpoint taqman methods for determining zygosity of corn comprising tc1507 events
WO2011143344A2 (en) 2010-05-12 2011-11-17 Agrigenetics, Inc. Use of brown midrib-3 gene specific markers in maize for trait introgression
US10117411B2 (en) * 2010-10-06 2018-11-06 Dow Agrosciences Llc Maize cytoplasmic male sterility (CMS) C-type restorer RF4 gene, molecular markers and their use

Also Published As

Publication number Publication date
CA2879860C (en) 2023-02-28
MX2015001135A (es) 2015-09-07
CA2879860A1 (en) 2014-01-30
EP2875155B1 (en) 2019-01-16
US20160355894A9 (en) 2016-12-08
US10214783B2 (en) 2019-02-26
BR112015001587B8 (pt) 2023-05-16
BR112015001587A2 (pt) 2017-08-01
EP2875155A1 (en) 2015-05-27
US20140024037A1 (en) 2014-01-23
BR112015001587B1 (pt) 2022-06-28
WO2014018501A1 (en) 2014-01-30
MX363287B (es) 2019-03-19

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