CL2011003186A1 - Metodo para la cuantificacion del evento bt11 en una muestra biologica de maiz basado en el uso de rt-pcr, partidores utilizados en dicho procedimiento, y sonda de polinucleotido. - Google Patents

Metodo para la cuantificacion del evento bt11 en una muestra biologica de maiz basado en el uso de rt-pcr, partidores utilizados en dicho procedimiento, y sonda de polinucleotido.

Info

Publication number
CL2011003186A1
CL2011003186A1 CL2011003186A CL2011003186A CL2011003186A1 CL 2011003186 A1 CL2011003186 A1 CL 2011003186A1 CL 2011003186 A CL2011003186 A CL 2011003186A CL 2011003186 A CL2011003186 A CL 2011003186A CL 2011003186 A1 CL2011003186 A1 CL 2011003186A1
Authority
CL
Chile
Prior art keywords
pcr
event
biological sample
quantification
procedure
Prior art date
Application number
CL2011003186A
Other languages
English (en)
Inventor
Hope Hart
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of CL2011003186A1 publication Critical patent/CL2011003186A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método mejorado para cuantificación de ADN en una muestra del evento BT11 en una muestra biológica de maíz; partidores de PCR; sonda de polinucleótido.
CL2011003186A 2009-06-18 2011-12-15 Metodo para la cuantificacion del evento bt11 en una muestra biologica de maiz basado en el uso de rt-pcr, partidores utilizados en dicho procedimiento, y sonda de polinucleotido. CL2011003186A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21815509P 2009-06-18 2009-06-18

Publications (1)

Publication Number Publication Date
CL2011003186A1 true CL2011003186A1 (es) 2012-07-13

Family

ID=43356766

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2011003186A CL2011003186A1 (es) 2009-06-18 2011-12-15 Metodo para la cuantificacion del evento bt11 en una muestra biologica de maiz basado en el uso de rt-pcr, partidores utilizados en dicho procedimiento, y sonda de polinucleotido.

Country Status (17)

Country Link
US (1) US9096896B2 (es)
EP (1) EP2443247B1 (es)
KR (1) KR20120044975A (es)
CN (1) CN102482697B (es)
AP (1) AP3139A (es)
AU (1) AU2010262786A1 (es)
BR (1) BRPI1011662A2 (es)
CA (1) CA2765958A1 (es)
CL (1) CL2011003186A1 (es)
CO (1) CO6480907A2 (es)
EA (1) EA021136B1 (es)
ES (1) ES2483121T3 (es)
MX (1) MX2011013199A (es)
NZ (1) NZ596987A (es)
PT (1) PT2443247E (es)
UA (1) UA105044C2 (es)
WO (1) WO2010148268A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3843771A4 (en) * 2018-08-27 2022-10-12 Syngenta Participations Ag COMPOSITIONS AND METHODS FOR PROTEIN DETECTION

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993023574A1 (en) * 1992-05-14 1993-11-25 Kozal Michael J Polymerase chain reaction assays for monitoring antiviral therapy
CN1157478C (zh) * 1996-09-03 2004-07-14 拜尔生物科学股份有限公司 改进的芽孢杆菌rna酶抑制剂基因
US20080034453A1 (en) 1999-05-06 2008-02-07 Nordine Cheikh Annotated Plant Genes
CN1584049A (zh) * 2003-08-22 2005-02-23 国家质量监督检验检疫总局动植物检疫实验所 转基因产品检测寡核苷酸芯片的制作方法及其应用
MX2007010036A (es) * 2005-03-16 2007-10-04 Syngenta Participations Ag Maiz evento 3272 y metodos para la deteccion del mismo.
EP1724360A1 (en) * 2005-05-17 2006-11-22 Eppendorf Array Technologies S.A. Identification and/or quantification method of nucleotide sequence(s) elements specific of genetically modified plants on arrays
WO2007019532A2 (en) * 2005-08-04 2007-02-15 North Carolina State University Peptide aptamers that bind to the rep proteins of ssdna viruses

Also Published As

Publication number Publication date
AP3139A (en) 2015-02-28
CO6480907A2 (es) 2012-07-16
UA105044C2 (uk) 2014-04-10
WO2010148268A1 (en) 2010-12-23
EA021136B1 (ru) 2015-04-30
CA2765958A1 (en) 2010-12-23
NZ596987A (en) 2013-04-26
ES2483121T3 (es) 2014-08-05
CN102482697B (zh) 2014-04-09
CN102482697A (zh) 2012-05-30
MX2011013199A (es) 2012-01-09
US9096896B2 (en) 2015-08-04
AP2011006051A0 (en) 2011-12-31
BRPI1011662A2 (pt) 2016-10-25
EP2443247A1 (en) 2012-04-25
US20120100544A1 (en) 2012-04-26
EA201200026A1 (ru) 2012-07-30
KR20120044975A (ko) 2012-05-08
EP2443247B1 (en) 2014-06-04
EP2443247A4 (en) 2012-11-21
AU2010262786A1 (en) 2012-01-12
PT2443247E (pt) 2014-07-18

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