AR086914A1 - Procedimientos de purificacion de formas nativas o mutantes de la toxina difterica - Google Patents
Procedimientos de purificacion de formas nativas o mutantes de la toxina diftericaInfo
- Publication number
- AR086914A1 AR086914A1 ARP120102087A ARP120102087A AR086914A1 AR 086914 A1 AR086914 A1 AR 086914A1 AR P120102087 A ARP120102087 A AR P120102087A AR P120102087 A ARP120102087 A AR P120102087A AR 086914 A1 AR086914 A1 AR 086914A1
- Authority
- AR
- Argentina
- Prior art keywords
- diphtheria toxin
- separating agent
- mutant
- hydroxyapatite
- agent
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1077—Pentosyltransferases (2.4.2)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente se refiere al uso de cromatografía de hidroxiapatita y cromatografía multimodal para la purificación de la toxina diftérica, o una forma mutante del mismo, de una mezcla, por ejemplo, una mezcla de fermentación en célula huésped que contiene impurezas, tales como proteínas y ADN de la célula huésped. La presente se refiere además a la integración de dicho procedimiento en un procedimiento de múltiples etapas con otros procedimientos de fraccionamiento para la purificación de la toxina diftérica adecuados para aplicaciones in vitro e in vivo.Reivindicación 1: Un procedimiento de purificar la toxina diftérica, o un mutante de la misma, de una mezcla que contiene la toxina diftérica, o una forma mutante de la misma, que comprende: a) poner en contacto la mezcla con un primer agente de separación en condiciones tales que la toxina diftérica, o un mutante de la misma, se une al primer agente de separación; b) eluir la toxina diftérica, o un mutante de la misma, del primer agente de separación; c) poner en contacto el material eluido obtenido en la tapa a) con un segundo agente de separación en condiciones tales que la toxina diftérica, o un mutante de la misma, se une al segundo agente de separación; y d) eluir dicha toxina diftérica, o un mutante de la misma, del segundo agente de separación; en el que 1) el primer agente de separación es hidroxiapatita y el segundo agente de separación es una resma multimodal; o 2) el primer agente de separación es resina multimodal y el segundo agente de separación es hidroxiapatita; y cuando el primer agente de separación o el segundo agente de separación es hidroxiapatita, antes de eluir de la hidroxiapatita, la hidroxiapatita sufre una etapa de lavado en condiciones tales que se eliminan las impurezas.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161496276P | 2011-06-13 | 2011-06-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR086914A1 true AR086914A1 (es) | 2014-01-29 |
Family
ID=47357422
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP120102087A AR086914A1 (es) | 2011-06-13 | 2012-06-12 | Procedimientos de purificacion de formas nativas o mutantes de la toxina difterica |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20140193876A1 (es) |
| EP (1) | EP2718306A1 (es) |
| KR (1) | KR20140038517A (es) |
| CN (1) | CN103732610A (es) |
| AR (1) | AR086914A1 (es) |
| BR (1) | BR112013032225A2 (es) |
| MX (1) | MX2013014773A (es) |
| TW (1) | TW201309722A (es) |
| WO (1) | WO2012173876A1 (es) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8188242B2 (en) * | 2008-04-08 | 2012-05-29 | Bio-Rad Laboratories, Inc. | Chromatography purification of antibodies |
| KR101871683B1 (ko) | 2010-07-30 | 2018-06-27 | 이엠디 밀리포어 코포레이션 | 크로마토그래피 매질 및 방법 |
| US20140228539A1 (en) * | 2011-10-21 | 2014-08-14 | Tanvex Biologics Corp. | Separation of acetylated proteins from unacetylated proteins |
| CN111172135A (zh) | 2012-10-24 | 2020-05-19 | 建新公司 | 使用mes和mops作为流动相改性剂从多峰树脂洗脱生物分子 |
| CN103266125A (zh) * | 2013-06-04 | 2013-08-28 | 北京民海生物科技有限公司 | 白喉毒素突变体crm197的制备方法 |
| US10329323B2 (en) * | 2014-07-25 | 2019-06-25 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Method for purifying antibodies using PBS |
| KR102162753B1 (ko) | 2014-12-08 | 2020-10-07 | 이엠디 밀리포어 코포레이션 | 혼합층 이온 교환 흡착제 |
| SG11201804083QA (en) * | 2015-11-18 | 2018-06-28 | Merck Patent Gmbh | Opposite ph-salt gradients for improved protein separations |
| US10934366B2 (en) | 2016-09-29 | 2021-03-02 | Bio-Rad Laboratories, Inc. | Protein-nanoparticle conjugate purification methods |
| EP3576759A4 (en) | 2017-01-31 | 2020-11-11 | Merck Sharp & Dohme Corp. | PROCESS FOR THE PREPARATION OF CAPSULE POLYSACCHARIDE PROTEIN CONJUGATES FROM STREPTOCOCCUS PNEUMONIAE SEROTYPE 19F |
| CN107043431B (zh) * | 2017-02-23 | 2020-06-30 | 上海瑞宙生物科技有限公司 | 细菌性荚膜多糖的纯化方法 |
| WO2018187388A1 (en) * | 2017-04-04 | 2018-10-11 | Scarab Genomics, Llc | Improved purification of crm 197 from bacteria |
| CN107163111A (zh) * | 2017-06-15 | 2017-09-15 | 华兰生物工程股份有限公司 | 纯化白喉毒素的方法 |
| CA3072129A1 (en) * | 2017-08-30 | 2019-03-07 | Ares Trading S.A. | Method for purifying proteins |
| AU2018328037B2 (en) | 2017-09-07 | 2024-03-07 | Merck Sharp & Dohme Llc | Pneumococcal polysaccharides and their use in immunogenic polysaccharide-carrier protein conjugates |
| SG11202005255PA (en) | 2017-12-06 | 2020-07-29 | Merck Sharp & Dohme | Compositions comprising streptococcus pneumoniae polysaccharide-protein conjugates and methods of use thereof |
| WO2019178587A1 (en) * | 2018-03-16 | 2019-09-19 | Bio-Rad Laboratories, Inc. | Purification of target molecules |
| TWI788610B (zh) | 2018-12-19 | 2023-01-01 | 美商默沙東有限責任公司 | 包含肺炎鏈球菌多醣-蛋白質結合物之組合物及其使用方法 |
| TW202245835A (zh) | 2021-02-04 | 2022-12-01 | 美商默沙東有限責任公司 | 用於肺炎球菌結合物疫苗之奈米乳化液佐劑組合物 |
| CN112851775B (zh) * | 2021-02-07 | 2022-02-18 | 艾美卫信生物药业（浙江）有限公司 | 一种白喉毒素无毒突变体crm197蛋白、生产方法以及应用 |
| CN115197311A (zh) * | 2021-04-09 | 2022-10-18 | 康立泰生物医药(青岛)有限公司 | 一种重组人白介素12蛋白的纯化方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030027253A1 (en) * | 2000-11-28 | 2003-02-06 | Presnell Scott R. | Cytokine receptor zcytor19 |
| EP1718738A2 (en) * | 2004-02-23 | 2006-11-08 | Crucell Holland B.V. | Virus purification methods |
| US8092683B2 (en) * | 2007-01-10 | 2012-01-10 | Ge Healthcare Bio-Sciences Ab | Multi-modal ion exchange chromatography resins |
| GB0713880D0 (en) * | 2007-07-17 | 2007-08-29 | Novartis Ag | Conjugate purification |
-
2012
- 2012-06-08 CN CN201280039610.1A patent/CN103732610A/zh active Pending
- 2012-06-08 BR BR112013032225A patent/BR112013032225A2/pt not_active IP Right Cessation
- 2012-06-08 KR KR1020147000714A patent/KR20140038517A/ko not_active Application Discontinuation
- 2012-06-08 MX MX2013014773A patent/MX2013014773A/es not_active Application Discontinuation
- 2012-06-08 EP EP12800460.3A patent/EP2718306A1/en not_active Withdrawn
- 2012-06-08 WO PCT/US2012/041444 patent/WO2012173876A1/en active Application Filing
- 2012-06-08 US US14/125,695 patent/US20140193876A1/en not_active Abandoned
- 2012-06-12 AR ARP120102087A patent/AR086914A1/es not_active Application Discontinuation
- 2012-06-13 TW TW101121030A patent/TW201309722A/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20140193876A1 (en) | 2014-07-10 |
| MX2013014773A (es) | 2014-01-20 |
| BR112013032225A2 (pt) | 2016-08-16 |
| TW201309722A (zh) | 2013-03-01 |
| CN103732610A (zh) | 2014-04-16 |
| WO2012173876A1 (en) | 2012-12-20 |
| KR20140038517A (ko) | 2014-03-28 |
| EP2718306A1 (en) | 2014-04-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |