AR053403A1 - Secuencias de reconocimiento de proteasas especificas para insectos - Google Patents

Secuencias de reconocimiento de proteasas especificas para insectos

Info

Publication number
AR053403A1
AR053403A1 ARP060102379A ARP060102379A AR053403A1 AR 053403 A1 AR053403 A1 AR 053403A1 AR P060102379 A ARP060102379 A AR P060102379A AR P060102379 A ARP060102379 A AR P060102379A AR 053403 A1 AR053403 A1 AR 053403A1
Authority
AR
Argentina
Prior art keywords
insect
modified
toxin
site
proteolytic activation
Prior art date
Application number
ARP060102379A
Other languages
English (en)
Original Assignee
Du Pont
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Pioneer Hi Bred Int filed Critical Du Pont
Publication of AR053403A1 publication Critical patent/AR053403A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Agronomy & Crop Science (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se proporcionan composiciones y métodos para proteger una planta de un insecto plaga. Se proporcionan moléculas de ácidos nucleicos que codifican protoxinas de insectos o toxinas de insectos modificadas para que comprendan al menos un sitio de activacion proteolítica que es sensible a una proteasa intestinal de los insectos. El corte de una protoxina de insecto modificada en el sitio de activacion proteolítica por una proteasa intestinal de los insectos produce una toxina de insecto activa en el intestino del insecto plaga. El corte de una toxina de insecto modificada de la presente en un sitio de activacion proteolítica resulta en la produccion de una toxina de insecto activa en el intestino del insecto, que presenta una actividad plaguicida mejorada respecto de la toxina del insecto que carece del sitio de activacion proteolítica. Se proporcionan métodos para usar las secuencias de la protoxina de insecto modificada y la toxina de insecto modificada, y los polipéptidos que codifican, para proteger la planta el dano provocado por el insecto plaga. En realizaciones particulares de la presente se proporcionan otras composiciones y formulaciones de protoxinas de insectos modificadas y toxinas de insectos modificadas, cassettes de expresion y plantas, células vegetales y semillas transformadas. En la presente documentacion también se describen proteasa intestinales de insectos y moléculas de ácidos nucleicos que las codifican.
ARP060102379A 2005-06-08 2006-06-07 Secuencias de reconocimiento de proteasas especificas para insectos AR053403A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68863505P 2005-06-08 2005-06-08
US72278705P 2005-09-30 2005-09-30

Publications (1)

Publication Number Publication Date
AR053403A1 true AR053403A1 (es) 2007-05-02

Family

ID=37142618

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP060102379A AR053403A1 (es) 2005-06-08 2006-06-07 Secuencias de reconocimiento de proteasas especificas para insectos

Country Status (8)

Country Link
US (1) US7473821B2 (es)
EP (1) EP1893638B1 (es)
AR (1) AR053403A1 (es)
AT (1) ATE519778T1 (es)
BR (1) BRPI0611681B1 (es)
CA (1) CA2611539C (es)
MX (1) MX2007015538A (es)
WO (1) WO2006133182A2 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7605304B2 (en) * 2000-10-24 2009-10-20 E.I. Du Pont De Nemours And Company Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans
WO2005063996A2 (en) * 2003-12-23 2005-07-14 Pioneer Hi-Bred International, Inc. Plant activation of insect toxin
US8609936B2 (en) 2007-04-27 2013-12-17 Monsanto Technology Llc Hemipteran-and coleopteran active toxin proteins from Bacillus thuringiensis
WO2009023636A1 (en) * 2007-08-10 2009-02-19 The University Of Georgia Research Foundation, Inc. Novel insect cadherin fragments
US7968525B1 (en) 2007-12-03 2011-06-28 University Of Florida Research Foundation, Inc. Use of RNA interference to validate new termiticide target sites and a method of termite control
EP2240030A1 (en) * 2008-01-10 2010-10-20 Pioneer Hi-Bred International Inc. Novel bacillus thuringiensis gene with coleopteran activity
US8269069B1 (en) 2008-07-30 2012-09-18 Dow Agrosciences, Llc Modified Bacillus thuringiensis cry proteins that inhibit coleopterans
AR073207A1 (es) * 2008-08-25 2010-10-20 Dow Agrosciences Llc Genes y proteinas cry de bacillus thuringiensis modificados para el control de nematodos
BRPI0917185A2 (pt) * 2008-08-25 2015-07-28 Dow Agrosciences Llc Proteínas cry12 do bacillus thuringiensis modificadas para o controle de nematódeo
US20110225681A1 (en) * 2008-08-25 2011-09-15 Dow Agrosciences Llc Modified Bacillus Thuringiensis Cry6 Proteins For Nematode Control
WO2010027799A1 (en) * 2008-08-25 2010-03-11 Dow Agrosciences Llc Modified bacillus thuringiensis cry5 proteins for nematode control
WO2011009182A2 (pt) 2009-07-24 2011-01-27 Embrapa - Empresa Brasileira De Pesquisa Agropecuária Molécula de ácido nucléico isolada, construção gênica, vetor, célula transgênica, método para obtenção de uma célula e de uma planta transgênica, polipeptídeo isolado e purificado, composição pesticida biodegradável, método para o controle de uma praga, método de obtenção de linhagens transgênicas resistentes a um inseto praga
AU2010339916B2 (en) * 2009-12-16 2016-04-21 Dow Agrosciences Llc Modified Cry1CA insecticidal Cry proteins
EP3495379A1 (en) 2012-04-06 2019-06-12 Monsanto Technology LLC Proteins toxic to hemipteran insect species
BR112015005389B1 (pt) * 2012-09-13 2022-10-18 Indiana University Research And Technology Corporation Molécula de ácido nucleico recombinante, proteína de substrato modificada de uma protease específica de patógeno de planta expressa através do patógeno de planta, vetor, e método para proteger uma planta da infecção por um patógeno de planta que secreta pelo menos uma protease específica
CA2957803A1 (en) 2014-09-11 2016-03-17 Marrone Bio Innovations, Inc. Chromobacterium subtsugae genome
US10612037B2 (en) 2016-06-20 2020-04-07 Monsanto Technology Llc Insecticidal proteins toxic or inhibitory to hemipteran pests
CN113610108B (zh) * 2021-07-06 2022-05-20 中南民族大学 一种基于改进残差网络的水稻害虫识别方法
WO2023058032A1 (en) * 2021-10-08 2023-04-13 Adama Agan Ltd. Novel formulation systems of carboxylic acid herbicides

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436002A (en) * 1991-01-29 1995-07-25 Mycogen Corporation Bacillus thuringiensisisolate PS201T6 toxin
US20020038005A1 (en) * 2000-01-07 2002-03-28 Wojciechowska Jana Alexandrovna Novel delta-endotoxins and nucleic acid sequences coding therefor
US7605304B2 (en) * 2000-10-24 2009-10-20 E.I. Du Pont De Nemours And Company Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans
US7230167B2 (en) * 2001-08-31 2007-06-12 Syngenta Participations Ag Modified Cry3A toxins and nucleic acid sequences coding therefor
WO2004000314A1 (en) * 2002-06-21 2003-12-31 Qlt Inc. Methods of using benzothiophenone derivatives to treat cancer or inflammation
AU2003279760A1 (en) * 2002-06-26 2004-01-19 E. I. Du Pont De Nemours And Company Genes encoding proteins with pesticidal activity
US7462760B2 (en) * 2002-06-26 2008-12-09 Pioneer Hi-Bred International, Inc. Genes encoding plant protease-resistant pesticidal proteins and method of their use
WO2005063996A2 (en) * 2003-12-23 2005-07-14 Pioneer Hi-Bred International, Inc. Plant activation of insect toxin

Also Published As

Publication number Publication date
US7473821B2 (en) 2009-01-06
BRPI0611681A2 (pt) 2010-09-28
BRPI0611681B1 (pt) 2018-01-16
CA2611539C (en) 2017-02-28
WO2006133182A3 (en) 2007-02-08
CA2611539A1 (en) 2006-12-14
MX2007015538A (es) 2008-03-06
US20060288448A1 (en) 2006-12-21
WO2006133182A2 (en) 2006-12-14
EP1893638B1 (en) 2011-08-10
ATE519778T1 (de) 2011-08-15
EP1893638A2 (en) 2008-03-05

Similar Documents

Publication Publication Date Title
AR053403A1 (es) Secuencias de reconocimiento de proteasas especificas para insectos
AR109260A2 (es) Secuencias de nucleótidos que codifican proteínas insecticidas
AR047350A1 (es) Genes que codifican proteinas con actividad plaguicida
NI201700044A (es) Proteínas quiméricas insecticidas novedosas tóxicas o inhibidoras de plagas de lepidópteros.
AR021067A1 (es) Composiciones polipeptidicas toxicas para insectos del genero diabrotica y metodos de uso de las mismas
AR112565A1 (es) Polipéptidos bioactivos para mejorar la protección, crecimiento y productividad en plantas
AR077319A2 (es) Proteinas insecticidas
CL2012001630A1 (es) Celulas de planta que comprenden adn que codifica para las proteinas insecticidas cry1be y cry1ab, composiscion para controlar plagas de lépidoteros que comprende dichas celulas, y metodo para manejar el desarrollo de resistencia de un insecto a una toxina cry.
AR074509A1 (es) Gen de bacillus thuringiensis con actividad pesticida contra insectos del orden lepidoptera
CR20190367A (es) Proteínas toxinas pesticidas activas contra insectos lepidópteros
AR078964A1 (es) Gen de bacillus thuringiensis que codifica polipeptido con actividad pesticida contra lepidoptera
AR053505A1 (es) Algodon insecticida 1143-14a
AR054171A1 (es) Aumento de rendimiento en plantas con sobreexpresion de los genes accdp
UY38692A (es) Método para controlar malezas en plantas de arroz con tolerancia incrementada el herbicida imidazolinona y sulfoniluréa
HN2006042227A (es) Metodos y composiciones para ingenieria de cultivo y control de enfermedades
AR065121A1 (es) Plantas con rasgos aumentados relacionados con el rendimiento y/o resistencia incrementada al estres abiotico y un metodo para desarrollar las mismas
AR053022A1 (es) Metodo para mejorar el desarrollo de plantas tolerantes o resistentes a los nematodos
CL2019001541A1 (es) Proteínas de variantes de secuencias de aminoácidos de cry1da1 activas para lepidópteros (divisional solicitud 201700896)
CL2012000799A1 (es) Proteína cryba que tiene una solubilidad o actividad insecticida mejorada contra al menos el gusano barrenador europeo (ostrinia nubilalis); molecula de acido nucleico que la codifica; gen quimérico, vector y celula huesped de dicho acido nucleico; método de producción; composicion insecticida; metodo para producir una planta resistente a insectos; y metodo de control de un insecto lepidóptero.
AR054158A1 (es) Plantas con rendimiento aumentado y un metodo para prepararlo
UY36361A (es) Molécula de ácido nucleico recombinante, planta, semilla, composición y producto que la comprenden, método que la utiliza para controlar lepidópteros
AR060435A1 (es) Polipeptidos relacionados con esteres y metodos para su uso en plantas
BRPI0507613A (pt) expressão de plasminogênio e microplasminogênio em lentilha d'água
AR047167A1 (es) Activacion de toxinas contra insectos en las plantas
AR043583A1 (es) Procedimiento para aumentar la resistencia contra factores de estres en plantas

Legal Events

Date Code Title Description
FB Suspension of granting procedure