AR053403A1 - Secuencias de reconocimiento de proteasas especificas para insectos - Google Patents

Secuencias de reconocimiento de proteasas especificas para insectos

Info

Publication number
AR053403A1
AR053403A1 ARP060102379A ARP060102379A AR053403A1 AR 053403 A1 AR053403 A1 AR 053403A1 AR P060102379 A ARP060102379 A AR P060102379A AR P060102379 A ARP060102379 A AR P060102379A AR 053403 A1 AR053403 A1 AR 053403A1
Authority
AR
Argentina
Prior art keywords
insect
modified
toxin
site
proteolytic activation
Prior art date
Application number
ARP060102379A
Other languages
English (en)
Original Assignee
Du Pont
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Pioneer Hi Bred Int filed Critical Du Pont
Publication of AR053403A1 publication Critical patent/AR053403A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472Cysteine endopeptidases (3.4.22)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Agronomy & Crop Science (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se proporcionan composiciones y métodos para proteger una planta de un insecto plaga. Se proporcionan moléculas de ácidos nucleicos que codifican protoxinas de insectos o toxinas de insectos modificadas para que comprendan al menos un sitio de activacion proteolítica que es sensible a una proteasa intestinal de los insectos. El corte de una protoxina de insecto modificada en el sitio de activacion proteolítica por una proteasa intestinal de los insectos produce una toxina de insecto activa en el intestino del insecto plaga. El corte de una toxina de insecto modificada de la presente en un sitio de activacion proteolítica resulta en la produccion de una toxina de insecto activa en el intestino del insecto, que presenta una actividad plaguicida mejorada respecto de la toxina del insecto que carece del sitio de activacion proteolítica. Se proporcionan métodos para usar las secuencias de la protoxina de insecto modificada y la toxina de insecto modificada, y los polipéptidos que codifican, para proteger la planta el dano provocado por el insecto plaga. En realizaciones particulares de la presente se proporcionan otras composiciones y formulaciones de protoxinas de insectos modificadas y toxinas de insectos modificadas, cassettes de expresion y plantas, células vegetales y semillas transformadas. En la presente documentacion también se describen proteasa intestinales de insectos y moléculas de ácidos nucleicos que las codifican.
ARP060102379A 2005-06-08 2006-06-07 Secuencias de reconocimiento de proteasas especificas para insectos AR053403A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68863505P 2005-06-08 2005-06-08
US72278705P 2005-09-30 2005-09-30

Publications (1)

Publication Number Publication Date
AR053403A1 true AR053403A1 (es) 2007-05-02

Family

ID=37142618

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP060102379A AR053403A1 (es) 2005-06-08 2006-06-07 Secuencias de reconocimiento de proteasas especificas para insectos

Country Status (8)

Country Link
US (1) US7473821B2 (es)
EP (1) EP1893638B1 (es)
AR (1) AR053403A1 (es)
AT (1) ATE519778T1 (es)
BR (1) BRPI0611681B1 (es)
CA (1) CA2611539C (es)
MX (1) MX2007015538A (es)
WO (1) WO2006133182A2 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7605304B2 (en) * 2000-10-24 2009-10-20 E.I. Du Pont De Nemours And Company Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans
BRPI0418365A (pt) * 2003-12-23 2007-05-15 Pioneer Hi Bred Int ativação por planta de toxina de inseto
US8609936B2 (en) 2007-04-27 2013-12-17 Monsanto Technology Llc Hemipteran-and coleopteran active toxin proteins from Bacillus thuringiensis
EP2190446A4 (en) * 2007-08-10 2010-09-08 Univ Georgia NEW INSECT CADHERINE FRAGMENTS
US7968525B1 (en) 2007-12-03 2011-06-28 University Of Florida Research Foundation, Inc. Use of RNA interference to validate new termiticide target sites and a method of termite control
EP2240030A1 (en) * 2008-01-10 2010-10-20 Pioneer Hi-Bred International Inc. Novel bacillus thuringiensis gene with coleopteran activity
US8269069B1 (en) 2008-07-30 2012-09-18 Dow Agrosciences, Llc Modified Bacillus thuringiensis cry proteins that inhibit coleopterans
BRPI0918840A2 (pt) * 2008-08-25 2015-08-18 Dow Agrosciences Llc Vetor de expressão de planta, proteína modificada, método de produção da referida proteína e método para inibição de um nematoide
WO2010027804A2 (en) * 2008-08-25 2010-03-11 Dow Agrosciences Llc Modified bacillus thuringiensis cry6 proteins for nematode control
BRPI0917185A2 (pt) * 2008-08-25 2015-07-28 Dow Agrosciences Llc Proteínas cry12 do bacillus thuringiensis modificadas para o controle de nematódeo
US20110239334A1 (en) * 2008-08-25 2011-09-29 Dow Agrosciences Llc Nematode-resistant plants, and modified bacillus thuringiensis cry genes and proteins
WO2011009182A2 (pt) 2009-07-24 2011-01-27 Embrapa - Empresa Brasileira De Pesquisa Agropecuária Molécula de ácido nucléico isolada, construção gênica, vetor, célula transgênica, método para obtenção de uma célula e de uma planta transgênica, polipeptídeo isolado e purificado, composição pesticida biodegradável, método para o controle de uma praga, método de obtenção de linhagens transgênicas resistentes a um inseto praga
KR101854478B1 (ko) * 2009-12-16 2018-05-03 다우 아그로사이언시즈 엘엘씨 변형 CRY1Ca 살곤충 CRY 단백질
CA2868815C (en) 2012-04-06 2017-10-24 Monsanto Technology Llc Proteins toxic to hemipteran insect species
WO2014042923A1 (en) 2012-09-13 2014-03-20 Indiana University Research & Technology Corporation Compositions and systems for conferring disease resistance in plants and methods of use thereof
WO2016039961A1 (en) 2014-09-11 2016-03-17 Marrone Bio Innovations, Inc Chromobacterium subtsugae genome
US10612037B2 (en) 2016-06-20 2020-04-07 Monsanto Technology Llc Insecticidal proteins toxic or inhibitory to hemipteran pests
CN113610108B (zh) * 2021-07-06 2022-05-20 中南民族大学 一种基于改进残差网络的水稻害虫识别方法
IL312048A (en) * 2021-10-08 2024-06-01 Adama Agan Ltd Preparations containing carboxylic acid herbicides

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436002A (en) * 1991-01-29 1995-07-25 Mycogen Corporation Bacillus thuringiensisisolate PS201T6 toxin
US20020038005A1 (en) * 2000-01-07 2002-03-28 Wojciechowska Jana Alexandrovna Novel delta-endotoxins and nucleic acid sequences coding therefor
US7605304B2 (en) * 2000-10-24 2009-10-20 E.I. Du Pont De Nemours And Company Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans
US7230167B2 (en) * 2001-08-31 2007-06-12 Syngenta Participations Ag Modified Cry3A toxins and nucleic acid sequences coding therefor
AU2003243863A1 (en) * 2002-06-21 2004-01-06 Qlt Inc. Methods of using benzothiophenone derivatives to treat cancer or inflammation
US7462760B2 (en) * 2002-06-26 2008-12-09 Pioneer Hi-Bred International, Inc. Genes encoding plant protease-resistant pesticidal proteins and method of their use
CA2490548A1 (en) * 2002-06-26 2004-01-08 E.I. Du Pont De Nemours And Company Genes encoding proteins with pesticidal activity
BRPI0418365A (pt) 2003-12-23 2007-05-15 Pioneer Hi Bred Int ativação por planta de toxina de inseto

Also Published As

Publication number Publication date
CA2611539A1 (en) 2006-12-14
EP1893638A2 (en) 2008-03-05
US20060288448A1 (en) 2006-12-21
WO2006133182A2 (en) 2006-12-14
WO2006133182A3 (en) 2007-02-08
BRPI0611681B1 (pt) 2018-01-16
US7473821B2 (en) 2009-01-06
BRPI0611681A2 (pt) 2010-09-28
EP1893638B1 (en) 2011-08-10
CA2611539C (en) 2017-02-28
ATE519778T1 (de) 2011-08-15
MX2007015538A (es) 2008-03-06

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