BRPI0507613A - expressão de plasminogênio e microplasminogênio em lentilha d'água - Google Patents

expressão de plasminogênio e microplasminogênio em lentilha d'água

Info

Publication number
BRPI0507613A
BRPI0507613A BRPI0507613-7A BRPI0507613A BRPI0507613A BR PI0507613 A BRPI0507613 A BR PI0507613A BR PI0507613 A BRPI0507613 A BR PI0507613A BR PI0507613 A BRPI0507613 A BR PI0507613A
Authority
BR
Brazil
Prior art keywords
plasminogen
microplasminogen
duckweed
expression
fragments
Prior art date
Application number
BRPI0507613-7A
Other languages
English (en)
Inventor
David Spencer
Lynn F Dickey
John R Gasdaska
Xiaowei Wang
Kevin M Cox
Charles G Peele
Original Assignee
Biolex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biolex Inc filed Critical Biolex Inc
Publication of BRPI0507613A publication Critical patent/BRPI0507613A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

EXPRESSãO DE PLASMINOGêNIO E MICROPLASMINOGêNIO EM LENTILHA D'áGUA A presente invenção provê métodos e composições para produção de plasminogênio recombinante, microplasminogênio e fragmentos dos mesmos em um sistema de expressão de lentilha d'água. Foi verificado de acordo com presente invenção que um sistema de expressão de lentilha d'água pode ser usado para produzir altos níveis de plasminogênio e microplasminogênio. O plasminogênio e o microplasminogênio produzidos da lentilha d'água podem ser ativados para produzir um polipeptídeo possuindo atividade de protease. Assim, a invenção engloba métodos para expressão de plasminogênio, microplasminogênio e fragmentos dos mesmos em lentilha d'água, plantas de lentilha d'água que são transformadas com cassetes de expressão para a expressão de plasminogênio, microplasminogênio e fragmentos dos mesmos e ácidos nucléicos compreendendo seqüências de nucleotídeo codificando plasminogênio, microplasminogênio e fragmentos dos mesmos, onde essas seqüências de nucleotídeo são modificadas para melhorar sua expressão na lentilha d'água.
BRPI0507613-7A 2004-02-11 2005-02-11 expressão de plasminogênio e microplasminogênio em lentilha d'água BRPI0507613A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54348704P 2004-02-11 2004-02-11
PCT/US2005/004245 WO2005078109A2 (en) 2004-02-11 2005-02-11 Expression of plasminogen and microplasminogen in duckweed

Publications (1)

Publication Number Publication Date
BRPI0507613A true BRPI0507613A (pt) 2007-07-03

Family

ID=34860429

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0507613-7A BRPI0507613A (pt) 2004-02-11 2005-02-11 expressão de plasminogênio e microplasminogênio em lentilha d'água

Country Status (12)

Country Link
US (2) US7659445B2 (pt)
EP (1) EP1713921A2 (pt)
JP (1) JP2007521834A (pt)
KR (1) KR20070004706A (pt)
CN (1) CN1938427A (pt)
AU (1) AU2005212431B2 (pt)
BR (1) BRPI0507613A (pt)
CA (1) CA2555609A1 (pt)
IL (1) IL177435A0 (pt)
NZ (1) NZ549537A (pt)
RU (1) RU2394103C2 (pt)
WO (1) WO2005078109A2 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7622573B2 (en) * 2006-01-17 2009-11-24 Biolex, Inc. Expression control elements from the lemnaceae family
KR100791090B1 (ko) 2007-01-05 2008-01-04 대한민국 플라스미노겐 액티베이터 유전자를 포함하는 식물 형질전환용 벡터 및 상기 벡터에 의해 형질전환된 식물
KR100887367B1 (ko) 2007-07-12 2009-03-06 대한민국(관리부서:농촌진흥청) 플라스미노겐 액티베이터 유전자를 포함하는 벡터, 상기벡터에 의해 형질전환된 모상근 및 이를 이용한플라스미노겐 액티베이터의 제조방법
WO2010138631A1 (en) * 2009-05-26 2010-12-02 Biolex Therapeutics, Inc. Compositions and methods for production of aglycosylated plasminogen
EP2445336A2 (en) 2009-06-23 2012-05-02 Biolex Therapeutics, Inc. Methods and compositions for the cryopreservation of duckweed
CA2767612A1 (en) 2009-07-10 2011-01-13 Thrombogenics Nv Variants of plasminogen and plasmin
WO2012093132A1 (en) 2011-01-05 2012-07-12 Thrombogenics Nv Plasminogen and plasmin variants
CN103764163A (zh) 2011-08-12 2014-04-30 斯路姆基因公司 纤溶酶原和纤溶酶变体
CN110066783B (zh) * 2019-05-16 2021-07-13 重庆派金生物科技有限公司 一种无自切形式的微纤溶酶制备方法
JP2022540865A (ja) * 2019-07-12 2022-09-20 モナッシュ ユニバーシティ 組換えタンパク質を作製するための方法
KR102251640B1 (ko) * 2019-11-27 2021-05-17 서울대학교산학협력단 지방세포 분화 촉진용 펩타이드 및 이를 포함하는 지방세포 분화 촉진용 조성물

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5648254A (en) * 1988-01-15 1997-07-15 Zymogenetics, Inc. Co-expression in eukaryotic cells
US6040498A (en) * 1998-08-11 2000-03-21 North Caroline State University Genetically engineered duckweed
US7161064B2 (en) * 1997-08-12 2007-01-09 North Carolina State University Method for producing stably transformed duckweed using microprojectile bombardment
US8022270B2 (en) * 2000-07-31 2011-09-20 Biolex Therapeutics, Inc. Expression of biologically active polypeptides in duckweed
CA2417415C (en) * 2000-07-31 2012-10-09 Biolex, Inc. Expression of biologically active polypeptides in duckweed
JP4047170B2 (ja) 2000-12-21 2008-02-13 トロム−イクス・ナムローゼ・フエンノートシャップ 酵母発現ベクターおよび酵母細胞における発現による組換えタンパク質の製造方法
ZA200306406B (en) 2001-01-19 2004-09-06 Dow Chemical Co Method for secretory production of glycoprotein having human-type sugar chain using plant cell.
CN1541059A (zh) 2001-03-06 2004-10-27 �Ϻ���ͨ��ѧ 具有动物型糖链加成功能的植物细胞
JP2003235561A (ja) * 2002-02-21 2003-08-26 Kazuhito Fujiyama 植物型糖鎖を持つ糖タンパク質を動物型糖鎖を持つ糖タンパク質に変換する方法
WO2005005643A2 (en) * 2003-07-01 2005-01-20 Biolex, Inc. Chloroplast transformation of duckweed
MXPA06012236A (es) 2004-04-22 2007-01-31 Talecris Biotherapeutics Inc Plasmina modificada en forma recombinante.

Also Published As

Publication number Publication date
WO2005078109A2 (en) 2005-08-25
RU2394103C2 (ru) 2010-07-10
WO2005078109A3 (en) 2005-11-10
US8017836B2 (en) 2011-09-13
US20050262592A1 (en) 2005-11-24
US7659445B2 (en) 2010-02-09
NZ549537A (en) 2009-09-25
AU2005212431A1 (en) 2005-08-25
EP1713921A2 (en) 2006-10-25
CA2555609A1 (en) 2005-08-25
CN1938427A (zh) 2007-03-28
IL177435A0 (en) 2006-12-10
JP2007521834A (ja) 2007-08-09
US20100186126A1 (en) 2010-07-22
KR20070004706A (ko) 2007-01-09
AU2005212431B2 (en) 2010-07-15
RU2006132394A (ru) 2008-03-20

Similar Documents

Publication Publication Date Title
BRPI0507613A (pt) expressão de plasminogênio e microplasminogênio em lentilha d'água
AR064216A1 (es) Polipeptidos cristalinos de bacillus thuringiensis, polinucleotidos y composiciones de los mismos
ES2553331T3 (es) Nuevos genes marcadores seleccionables
CY1117351T1 (el) Κυτταροδιαπερατοι αναστολεις πεπτιδιων της jnk-σηματοδοτικης πορειας μεταγωγης
AR040446A1 (es) Defensinas de plantas
AR060141A1 (es) Proteinas que se relacionan con la respuesta al estres abiotico y homologos
BR0313536A (pt) Sequencias de ácido nucléico de diacilglicerol acetiltransferase e produtos associados
ECSP088262A (es) Proteínas de fusión de albúmina
BR0113864A (pt) ácidos nucléicos tipo kcp isolados e suas proteìnas codificadas
BRPI0511868A (pt) peptìdeo isolado, polipeptìdeo de fusão, moléculas de ácido nucléico isoladas, vetores, métodos de direcionamento de polipeptìdeos e método de identificação de peptìdeos
AR059257A1 (es) Genes para mejorar la eficiencia de la utilizacion de nitrogeno en los cultivos
CL2012000063A1 (es) Polipéptido factor ix (fix) o un fragmento del mismo porque comprende la modificación de un aminoácido; vector; célula; método para expresar el polipéptido fix; composición farmacéutica; uso de la composición para tratar enfermedades trombóticas, hemorrágicas y coagulación intravascular diseminada (cid) (div. sol. 1796-07).
AR049570A1 (es) Polipeptidos antifungicos para plantas
BRPI0606803A2 (pt) axmi-018, axmi-020 e axmi-021, uma famìlia de genes de delta-endotoxina e métodos para seu uso
BR0211035A (pt) Fragmento nucleotìdico isolado, complemento, construção de dna recombinante, planta, sementes, óleo, métodos para alteração de fenótipo, método de isolamento de fragmentos de ácidos nucléicos, método de mapeamento e método de cultura molecular
BR112012033404A2 (pt) polipetídeo tendo ou auxiliando a atividade de degradação de material de carboidratos e uso dos mesmos.
MX2009002816A (es) Proteinas de fusion de albumina.
AR068568A1 (es) Gen de resistencia a bs3 y metodos de uso
NO20084577L (no) Probiotisk stamme og antimikrobielle peptider fra disse
AR036962A1 (es) Metiltransferasas aromaticas y usos de las mismas
NO20055210D0 (no) Inhibitorproteiner av en protease og anvendelse derav
AR072495A1 (es) Secuencia reguladora de las plantas proveniente de un gen de maiz
ECSP10010082A (es) Udp-glucuronil transferasa y polinucleotido de codificacion
BR112012033714A2 (pt) polipeptídeo com atividade de acetil xilano esterase e seus usos.
BRPI0513267A (pt) variantes melhoradas de aprotinina

Legal Events

Date Code Title Description
B25D Requested change of name of applicant approved

Owner name: BIOLEX THERAPEUTICS, INC. (US)

Free format text: ALTERADO DE: BIOLEX INC.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B25A Requested transfer of rights approved

Owner name: SYNTHON BIOPHARMACEUTICALS B.V (NL)

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]