AR048798A1 - Eventos de tolerancia a glifosato en alfalfa y metodos para su deteccion - Google Patents
Eventos de tolerancia a glifosato en alfalfa y metodos para su deteccionInfo
- Publication number
- AR048798A1 AR048798A1 ARP040100305A ARP040100305A AR048798A1 AR 048798 A1 AR048798 A1 AR 048798A1 AR P040100305 A ARP040100305 A AR P040100305A AR P040100305 A ARP040100305 A AR P040100305A AR 048798 A1 AR048798 A1 AR 048798A1
- Authority
- AR
- Argentina
- Prior art keywords
- dna
- alfalfa
- plant
- seq
- sample
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Physiology (AREA)
- Environmental Sciences (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Eventos de alfalfa J-101 y J-163, y moléculas de ADN unicas para estos eventos. Métodos para detectar la presencia de esta moléculas de ADN en una muestra vegetal. Reivindicacion 5: La planta de alfalfa J-101 de la reivindicacion 4, caracterizada porque el goenoma de dicha planta de alfalfa J-101 comprende una molécula de ADN que se selecciona del grupo que consiste en la SEQ ID Ns, SEQ ID Ns 2, SEQ ID Ns 3 y SEQ ID Ns 4. Reivindicacion 10: Un método para producir una planta que tolera la aplicacion del herbicida glifosato, carcterizado porque comprende (a) cruzar por vía sexual un primer progenitor de un evento de alfalfa con tolerancia a glifosato J-101, y un segundo progenitor de una planta de alfalfa que carece de tolerancia a glifosato, produciendo así una pluralidad de plantas de la progenie; y (b) seleccionar una planta de la progenie que tolera la aplicacion de glifosato; y (c) autocruzar dicha planta de la primera progenie, produciendo así una pluralidad de plantas de la segunda progenie; y (d) seleccionar entre dichas plantas de segunda progenie aquellas plantas que presentan tolerancia a glifosato. Reivindicacion 11: Un método para detectar la presencia de un ADN que corresponde a ADN de la planta de alfalfa J-101 en una muestra, caracterizado porque comprende: (a) poner la muestra que comprende ADN en contacto con un conjunto de cebadores que, cuando se lo usa en una reaccion de amplificacion de ácidos nucleicos con ADN genomico del evento de alfalfa J-101, produce un amplicon de ADN que comprende la SEQ ID Ns 1 o SEQ ID Ns 2; y (b) realizar una reaccion de amplificacion de ácidos nucleicos, produciendo así el amplicon; y (c) detectar el amplicon. Reivindicacion 12: Un método para detectar la presencia de un ADN que corresponde a alfalfa J-101 en una muestra, caracterizado porque comprende: (a) poner la muestra que comprende ADN en contacto con una sonda, que se hibridiza bajo condiciones de hibridacion estrictas con ADN genomico del evento de alfalfa J-101 y no se hibridiza bajo condiciones de hibridacion estrictas con ADN de una planta de alfalfa control, donde dicha sonda es homologa o complementaria con SEQ ID Ns 1 o SEQ ID Ns 2; y (b) someter la muestra y la sonda a condiciones de hibridacion estrictas; y (c) detectar la hibridacion de la sonda con el ADN.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US44399703P | 2003-01-31 | 2003-01-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR048798A1 true AR048798A1 (es) | 2006-05-31 |
Family
ID=32850816
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP040100305A AR048798A1 (es) | 2003-01-31 | 2004-02-02 | Eventos de tolerancia a glifosato en alfalfa y metodos para su deteccion |
| ARP170101885A AR114135A2 (es) | 2003-01-31 | 2017-07-07 | Eventos de tolerancia a glifosato en alfalfa y métodos para su detección |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP170101885A AR114135A2 (es) | 2003-01-31 | 2017-07-07 | Eventos de tolerancia a glifosato en alfalfa y métodos para su detección |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US7566817B2 (es) |
| EP (3) | EP2270189B1 (es) |
| AR (2) | AR048798A1 (es) |
| AT (1) | ATE553202T1 (es) |
| AU (1) | AU2004209383B2 (es) |
| BR (2) | BRPI0407173B1 (es) |
| CA (2) | CA2514450C (es) |
| DK (3) | DK1587931T3 (es) |
| MX (2) | MXPA05008143A (es) |
| WO (1) | WO2004070020A2 (es) |
Families Citing this family (76)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE553202T1 (de) | 2003-01-31 | 2012-04-15 | Monsanto Technology Llc | Glyphosat-tolerante luzernepflanzen und verfahren zu deren detektion |
| EP1729580B1 (en) | 2004-03-30 | 2018-04-11 | Monsanto Technology LLC | Methods for controlling plant pathogens using n-phosphonomethylglycine |
| AP2693A (en) | 2005-05-27 | 2013-07-16 | Monsanto Technology Llc | Soybean event MON89788 and methods for detection thereof |
| CA2651428A1 (en) * | 2006-05-12 | 2007-11-22 | Commonwealth Scientific And Industrial Research Organisation | Enzymes for degrading herbicides |
| RU2624025C2 (ru) * | 2009-09-17 | 2017-06-30 | МОНСАНТО ТЕКНОЛОДЖИ ЭлЭлСи | Трансгенный объект сои mon 87708 и способы его применения |
| KR101941297B1 (ko) | 2010-06-04 | 2019-01-22 | 몬산토 테크놀로지 엘엘씨 | 유전자 이식 브라씨카의 사건 mon 88302 및 이것의 사용방법 |
| US9493786B2 (en) * | 2010-10-12 | 2016-11-15 | Monsanto Technology Llc | Soybean plant and seed corresponding to transgenic event MON87712 comprising a B-box zinc finger protein 32, and methods for detection thereof |
| AU2012217821A1 (en) | 2011-02-14 | 2013-07-11 | Xyleco, Inc. | Processing biomass |
| CA2840630C (en) | 2011-06-30 | 2021-11-30 | Monsanto Technology Llc | Alfalfa plant and seed corresponding to transgenic event kk 179-2 and methods for detection thereof |
| US9854778B2 (en) | 2011-06-30 | 2018-01-02 | Monsanto Technology Llc | Alfalfa plant and seed corresponding to transgenic event KK 179-2 and methods for detection thereof |
| BR122019026534B1 (pt) | 2012-06-26 | 2022-07-19 | Monsanto Technology Llc | Método para selecionar uma planta alfafa com componentes de valor alimentar melhorados de uma população de plantas alfafa |
| PE20211797A1 (es) | 2014-03-20 | 2021-09-13 | Monsanto Technology Llc | Evento de maiz transgenico mon 87419 y metodos para su uso |
| CN104372088A (zh) * | 2014-11-11 | 2015-02-25 | 中华人民共和国黄埔出入境检验检疫局 | 转基因苜蓿草j101品系特异性定性pcr检测用引物及检测方法 |
| CN104561262B (zh) * | 2014-11-11 | 2017-04-19 | 黄埔出入境检验检疫局综合技术服务中心 | 转基因苜蓿草J101品系Taqman实时荧光定量PCR检测用引物和探针及检测方法 |
| CN104673886B (zh) * | 2014-11-11 | 2017-01-18 | 中华人民共和国黄埔出入境检验检疫局 | 转基因苜蓿草j163品系实时荧光pcr检测用引物和探针及检测方法和应用 |
| CN104372098A (zh) * | 2014-11-21 | 2015-02-25 | 中华人民共和国黄埔出入境检验检疫局 | 转基因苜蓿草j163品系特异性定性pcr检测用引物及检测方法 |
| CN104745685B (zh) * | 2015-01-23 | 2017-11-03 | 黄埔出入境检验检疫局综合技术服务中心 | 转基因苜蓿草j101品系特异性环介导等温扩增检测用引物及检测方法和应用 |
| JP2021501231A (ja) | 2017-10-27 | 2021-01-14 | キシレコ インコーポレイテッド | バイオマスの加工方法 |
| US10357011B1 (en) | 2018-03-01 | 2019-07-23 | Forage Genetics International, Llc | Alfalfa variety RRL43A108 |
| US10299460B1 (en) | 2018-03-01 | 2019-05-28 | Forage Genetics International, Llc | Alfalfa variety 68M802 |
| US10531630B2 (en) | 2018-03-01 | 2020-01-14 | Forage Genetics International, Llc | Alfalfa variety 410A176 |
| US10412925B1 (en) | 2018-03-01 | 2019-09-17 | Forage Genetics International, Llc | Alfalfa variety RRL43M104 |
| US10531633B2 (en) | 2018-03-01 | 2020-01-14 | Forage Genetics International, Llc | Alfalfa variety 49H344 |
| US10327413B1 (en) | 2018-03-01 | 2019-06-25 | Forage Genetics International, Llc | Alfalfa variety RRL913T455 |
| US10555495B2 (en) | 2018-03-01 | 2020-02-11 | Forage Genetics International, Llc | Alfalfa variety R410A136 |
| US10357013B1 (en) | 2018-03-01 | 2019-07-23 | Forage Genetics International, Llc | Alfalfa variety 412W201 |
| US10555496B2 (en) | 2018-03-01 | 2020-02-11 | Forage Genetics International, Llc | Alfalfa variety R410M327 |
| US10314279B1 (en) | 2018-03-01 | 2019-06-11 | Forage Genetics International, Llc | Alfalfa variety RRL43Q109 |
| US10357012B1 (en) | 2018-03-01 | 2019-07-23 | Forage Genetics International, Llc | Alfalfa variety RRL43M113 |
| US10327412B1 (en) | 2018-03-01 | 2019-06-25 | Forage Genetics International, Llc | Alfalfa variety RRL43M115 |
| US10299459B1 (en) | 2018-03-01 | 2019-05-28 | Forage Genetics International, Llc | Alfalfa variety 610W265 |
| US10555494B2 (en) | 2018-03-01 | 2020-02-11 | Forage Genetics International, Llc | Alfalfa variety R410A131 |
| US10314280B1 (en) | 2018-03-01 | 2019-06-11 | Forage Genetics International, Llc | Alfalfa variety 49W202 |
| US10485214B2 (en) | 2018-03-01 | 2019-11-26 | Forage Genetics International, Llc | Alfalfa variety R411A107 |
| US10342208B1 (en) | 2018-03-01 | 2019-07-09 | Forage Genetics International, Llc | Alfalfa variety RRL43M114 |
| US10555493B2 (en) | 2018-03-01 | 2020-02-11 | Forage Genetics International, Llc | Alfalfa variety R410A109 |
| US10531632B2 (en) | 2018-03-01 | 2020-01-14 | Forage Genetics International, Llc | Alfalfa variety 410A179 |
| US10334812B1 (en) | 2018-03-01 | 2019-07-02 | Forage Genetics International, Llc | Alfalfa variety RRL913T404 |
| US10342209B1 (en) | 2018-03-01 | 2019-07-09 | Forage Genetics International, Llc | Alfalfa variety RRL43M119 |
| US10531631B2 (en) | 2018-03-01 | 2020-01-14 | Forage Genetics International, Llc | Alfalfa variety 410A177 |
| US10757900B2 (en) | 2019-01-23 | 2020-09-01 | Forage Genetics International, Llc | Alfalfa variety H0415A3144 |
| US10681894B1 (en) | 2019-01-23 | 2020-06-16 | Forage Genetics International, Llc | Alfalfa variety 512W206 |
| US10827717B2 (en) | 2019-01-23 | 2020-11-10 | Forage Genetics International, Llc | Alfalfa variety R513M225S |
| US10687507B1 (en) | 2019-01-23 | 2020-06-23 | Forage Genetics International, Llc | Alfalfa variety 810T104 |
| US10624306B1 (en) | 2019-01-23 | 2020-04-21 | Forage Genetics International, Llc | Alfalfa variety 412A122 |
| US10687505B1 (en) | 2019-01-23 | 2020-06-23 | Forage Genetics International, Llc | Alfalfa variety 412A121 |
| US10772282B2 (en) | 2019-01-23 | 2020-09-15 | Forage Genetics International, Llc | Alfalfa variety R413A316 |
| US10681893B1 (en) | 2019-01-23 | 2020-06-16 | Forage Genetics International, Llc | Alfalfa variety 413H323 |
| US10736296B1 (en) | 2019-01-23 | 2020-08-11 | Forage Genetics International, Llc | Alfalfa variety H0515A3140 |
| US10925247B2 (en) | 2019-01-23 | 2021-02-23 | Forage Genetics International, Llc | Alfalfa variety RRL43M118 |
| US10757899B2 (en) | 2019-01-23 | 2020-09-01 | Forage Genetics International, Llc | Alfalfa variety 109T901 |
| US10827716B2 (en) | 2019-01-23 | 2020-11-10 | Forage Genetics International, Llc | Alfalfa variety R511Hg216 |
| US10757898B2 (en) | 2019-01-23 | 2020-09-01 | Forage Genetics International, Llc | Alfalfa variety 58M801 |
| US10757901B2 (en) | 2019-01-23 | 2020-09-01 | Forage Genetics International, Llc | Alfalfa variety 914T031 |
| US10939655B2 (en) | 2019-01-23 | 2021-03-09 | Forage Genetics International, Llc | Alfalfa variety R513W224S |
| US10932433B2 (en) | 2019-01-23 | 2021-03-02 | Forage Genetics International, Llc | Alfalfa variety RRL44M375 |
| US10757902B2 (en) | 2019-01-23 | 2020-09-01 | Forage Genetics International, Llc | Alfalfa variety 1013T183 |
| US10667486B1 (en) | 2019-01-23 | 2020-06-02 | Forage Genetics International, Llc | Alfalfa variety 1013M185 |
| US10674696B1 (en) | 2019-01-23 | 2020-06-09 | Forage Genetics International, Llc | Alfalfa variety 510M374 |
| US10798909B2 (en) | 2019-01-23 | 2020-10-13 | Forage Genetics International, Llc | Alfalfa variety RRL44M377 |
| US10674697B1 (en) | 2019-01-23 | 2020-06-09 | Forage Genetics International, Llc | Alfalfa variety 1114T029 |
| US10736297B1 (en) | 2019-01-23 | 2020-08-11 | Forage Genetics International, Llc | Alfalfa variety R411A106 |
| US10687506B1 (en) | 2019-01-23 | 2020-06-23 | Forage Genetics International, Llc | Alfalfa variety 510M172 |
| US10694712B1 (en) | 2019-01-23 | 2020-06-30 | Forage Genetics International, Llc | Alfalfa variety 89T093 |
| US10779504B2 (en) | 2019-01-23 | 2020-09-22 | Forage Genetics International, Llc | Alfalfa variety RRL44M121 |
| US10798908B2 (en) | 2019-01-23 | 2020-10-13 | Forage Genetics International, Llc | Alfalfa variety RRL44M104 |
| US10624307B1 (en) | 2019-01-23 | 2020-04-21 | Forage genetics Intenational, LLC | Alfalfa variety 412W204 |
| US10653100B1 (en) | 2019-01-23 | 2020-05-19 | Forage Genetics International, Llc | Alfalfa variety R410M324 |
| US10779503B2 (en) | 2019-01-23 | 2020-09-22 | Forage Genetics International, Llc | Alfalfa variety R411K324 |
| CN112080501B (zh) * | 2020-09-10 | 2021-11-23 | 隆平生物技术(海南)有限公司 | 重组启动子、基因表达盒及其在植物育种中的应用 |
| US11477963B2 (en) | 2020-12-23 | 2022-10-25 | Forage Genetics International, Llc | Alfalfa variety H0615T514 |
| US11330786B1 (en) | 2020-12-23 | 2022-05-17 | Forage Genetics International, Llc | Alfalfa variety H0316ML103 |
| US11516986B2 (en) | 2020-12-23 | 2022-12-06 | Forage Genetics International, Llc | Alfalfa variety C0316A3159 |
| US11252927B1 (en) | 2020-12-23 | 2022-02-22 | Forage Genetics International, Llc | Alfalfa variety C0516A3154 |
| US11516985B2 (en) | 2020-12-23 | 2022-12-06 | Forage Genetics International, Llc | Alfalfa variety 513M118 |
| US11516984B2 (en) | 2020-12-23 | 2022-12-06 | Forage Genetics International, Llc | Alfalfa variety R414H347 |
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| AU9304401A (en) | 2000-09-29 | 2002-04-08 | Monsanto Technology Llc | Glyphosate tolerant wheat plant 33391 and compositions and methods for detectionthereof |
| EP1366070A2 (en) * | 2000-10-25 | 2003-12-03 | Monsanto Technology LLC | Cotton event pv-ghgt07(1445) and compositions and methods for detection thereof |
| US7306909B2 (en) * | 2000-10-30 | 2007-12-11 | Monsanto Technology Llc | Canola event pv-bngt04(rt73) and compositions and methods for detection thereof |
| AU2002236442A1 (en) | 2000-11-20 | 2002-05-27 | Monsanto Technology Llc | Cotton event pv-ghbk04 (531) and compositions and methods for detection thereof |
| AU2002218413A1 (en) * | 2000-11-30 | 2002-06-11 | Ses Europe N.V. | Glyphosate resistant transgenic sugar beet characterised by a specific transgene insertion (t227-1), methods and primers for the detection of said insertion |
| ATE553202T1 (de) | 2003-01-31 | 2012-04-15 | Monsanto Technology Llc | Glyphosat-tolerante luzernepflanzen und verfahren zu deren detektion |
-
2004
- 2004-01-14 AT AT04702162T patent/ATE553202T1/de active
- 2004-01-14 CA CA2514450A patent/CA2514450C/en not_active Expired - Lifetime
- 2004-01-14 EP EP10179402.2A patent/EP2270189B1/en not_active Expired - Lifetime
- 2004-01-14 BR BRPI0407173-5A patent/BRPI0407173B1/pt active IP Right Grant
- 2004-01-14 DK DK04702162.1T patent/DK1587931T3/da active
- 2004-01-14 WO PCT/US2004/000812 patent/WO2004070020A2/en active Application Filing
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- 2004-01-14 DK DK10179402.2T patent/DK2270189T3/da active
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