AR048519A1 - Secuencias de citoquinina oxidasa y metodos de uso de las mismas - Google Patents

Secuencias de citoquinina oxidasa y metodos de uso de las mismas

Info

Publication number
AR048519A1
AR048519A1 ARP050101310A ARP050101310A AR048519A1 AR 048519 A1 AR048519 A1 AR 048519A1 AR P050101310 A ARP050101310 A AR P050101310A AR P050101310 A ARP050101310 A AR P050101310A AR 048519 A1 AR048519 A1 AR 048519A1
Authority
AR
Argentina
Prior art keywords
amino acid
modulation
acid sequence
seq
development
Prior art date
Application number
ARP050101310A
Other languages
English (en)
Original Assignee
Pionner Hi Bred Internat Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pionner Hi Bred Internat Inc filed Critical Pionner Hi Bred Internat Inc
Publication of AR048519A1 publication Critical patent/AR048519A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/22—Anxiolytics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8295—Cytokinins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Endocrinology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Psychiatry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Métodos y composiciones para modular el desarrollo vegetal. Se proveen secuencias de polinucleotidos y secuencias de aminoácidos que codifican polipéptidos de citoquinina oxidasa. Las secuencias se pueden usar en diversos métodos incluyendo la modulacion del desarrollo radicular, modulacion del desarrollo floral, modulacion del desarrollo foliar y/o de brotes, modulacion del tamano y/o el peso de las semillas, modulacion de la tolerancia bajo estrés abiotico y modulacion de la resistencia a patogenos. También se proveen polinucleotidos que comprenden promotores CKX. Los promotores se pueden usar para regular la expresion de una secuencia de interés. Se proveen asimismo plantas transformadas, células y tejidos vegetales y semillas. Reivindicacion 1: Un polipéptido aislado que comprende una secuencia de aminoácidos seleccionada del grupo formado por: (a) una secuencia de aminoácidos que comprende la SEQ ID No. :6, 9, 12 o 53; (b) una secuencia de aminoácidos que comprende al menos un 85% de identidad de secuencia con la SEQ ID No. : 6, 9, 12 o 53, donde dicho polipéptido posee actividad citoquinina oxidasa. (c) una secuencia de aminoácidoscodificada por una secuencia de nucleotidos que se hibridiza bajo condiciones severas con el complemento de la SEQ ID No. : 5, 8, 11 o 52, donde dichas condiciones severas comprenden hibridacion en formamida 50% NaCl 1 M, SDS 1% a 37 degree C y un lavado en SSC 0,1X a 60-65 degree C; y (d) una secuencia de aminoácidos que comprende al menos 17 aminoácidos consecutivos de la SEQ ID No. 6, 9, 12 o 53, donde dicho polipéptido retiene la actividad citoquinina oxidasa.
ARP050101310A 2004-04-02 2005-04-01 Secuencias de citoquinina oxidasa y metodos de uso de las mismas AR048519A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US55925204P 2004-04-02 2004-04-02

Publications (1)

Publication Number Publication Date
AR048519A1 true AR048519A1 (es) 2006-05-03

Family

ID=34964776

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050101310A AR048519A1 (es) 2004-04-02 2005-04-01 Secuencias de citoquinina oxidasa y metodos de uso de las mismas

Country Status (10)

Country Link
US (1) US20060021082A1 (es)
EP (1) EP1740606A2 (es)
AR (1) AR048519A1 (es)
AU (1) AU2005230820B2 (es)
BR (1) BRPI0509545A (es)
CA (1) CA2563344C (es)
CL (1) CL2009001972A1 (es)
MX (1) MXPA06011360A (es)
WO (1) WO2005097824A2 (es)
ZA (1) ZA200608285B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7468475B2 (en) 2000-06-16 2008-12-23 Schmuelling Thomas Method for modifying plant morphology, biochemistry and physiology
CN100554423C (zh) * 2006-01-05 2009-10-28 华中农业大学 一种控制水稻谷粒粒长和粒重的主效基因gs3
PL222498B1 (pl) * 2009-05-27 2016-08-31 Inst Hodowli I Aklimatyzacji Roślin Kaseta DNA, wektor binarny, szczep A. tumefaciens oraz sposób otrzymywania rośliny zbożowej o zwiększonej produktywności
CN109402078B (zh) * 2018-11-28 2021-05-18 浙江师范大学 一种水稻衰老控制基因OsCKX11及其应用
CN112243780B (zh) * 2020-10-20 2023-06-13 广西宇花灵科技发展有限公司 柑橘植株细胞液浓度调控免环割的栽培方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6229066B1 (en) * 1997-07-30 2001-05-08 The Curators Of The University Of Missouri Cytokinin oxidase
CA2370594C (en) * 1999-04-16 2007-01-09 Pioneer Hi-Bred International, Inc. Regulated expression of genes in plant seeds
US7531723B2 (en) * 1999-04-16 2009-05-12 Pioneer Hi-Bred International, Inc. Modulation of cytokinin activity in plants
US6992237B1 (en) * 1999-04-16 2006-01-31 Pioneer Hi-Bred International Inc. Regulated expression of genes in plant seeds
US6423885B1 (en) * 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
CN102174540B (zh) * 2000-06-16 2013-08-07 托马斯·施穆林 改变植物形态学、生物化学和生理学的方法
US6506696B2 (en) * 2001-03-26 2003-01-14 Air Products Polymers, L.P. High performance synthetic nonwovens using polymers having dual crosslinkable functionality
AU2002254415A1 (en) * 2001-03-29 2002-10-15 Pioneer Hi-Bred International, Inc. Tissue-preferred promoter from maize
US7619146B2 (en) * 2001-06-18 2009-11-17 Frankard Valerie Method for modifying plant morphology, biochemistry and physiology
CA2468753C (en) * 2001-12-10 2014-07-08 Thomas Schmulling Method for modifying plant morphology, biochemistry and physiology comprising expression of plant cytokinin oxidase
US7230168B2 (en) * 2001-12-20 2007-06-12 The Curators Of The University Of Missouri Reversible male sterility in transgenic plants by expression of cytokinin oxidase

Also Published As

Publication number Publication date
CA2563344A1 (en) 2005-10-20
WO2005097824A3 (en) 2006-04-20
WO2005097824A2 (en) 2005-10-20
AU2005230820A1 (en) 2005-10-20
CL2009001972A1 (es) 2010-03-12
BRPI0509545A (pt) 2007-09-18
CA2563344C (en) 2011-10-11
AU2005230820B2 (en) 2008-09-11
US20060021082A1 (en) 2006-01-26
MXPA06011360A (es) 2007-03-29
EP1740606A2 (en) 2007-01-10
ZA200608285B (en) 2008-04-30

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