AR036178A1 - Genes de la desaturasa del acido graso de la granada y procedimiento para la preparacion de acidos grasos insaturados - Google Patents
Genes de la desaturasa del acido graso de la granada y procedimiento para la preparacion de acidos grasos insaturadosInfo
- Publication number
- AR036178A1 AR036178A1 ARP020102694A ARP020102694A AR036178A1 AR 036178 A1 AR036178 A1 AR 036178A1 AR P020102694 A ARP020102694 A AR P020102694A AR P020102694 A ARP020102694 A AR P020102694A AR 036178 A1 AR036178 A1 AR 036178A1
- Authority
- AR
- Argentina
- Prior art keywords
- nucleic acid
- seq
- acid sequence
- unsaturated
- sequence
- Prior art date
Links
- 238000002360 preparation method Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 239000002253 acid Substances 0.000 title 2
- 150000007513 acids Chemical class 0.000 title 1
- 235000019197 fats Nutrition 0.000 title 1
- 235000021081 unsaturated fats Nutrition 0.000 title 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 16
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 10
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 235000014113 dietary fatty acids Nutrition 0.000 abstract 3
- 229930195729 fatty acid Natural products 0.000 abstract 3
- 239000000194 fatty acid Substances 0.000 abstract 3
- 150000004665 fatty acids Chemical class 0.000 abstract 3
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 150000003626 triacylglycerols Chemical class 0.000 abstract 3
- 150000004670 unsaturated fatty acids Chemical class 0.000 abstract 3
- 235000021122 unsaturated fatty acids Nutrition 0.000 abstract 3
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 2
- 229910052739 hydrogen Inorganic materials 0.000 abstract 2
- 239000001257 hydrogen Substances 0.000 abstract 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 abstract 2
- 229920006395 saturated elastomer Polymers 0.000 abstract 2
- 125000000008 (C1-C10) alkyl group Chemical group 0.000 abstract 1
- 125000006545 (C1-C9) alkyl group Chemical group 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 230000021615 conjugation Effects 0.000 abstract 1
- 239000002537 cosmetic Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 239000010685 fatty oil Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 239000003921 oil Substances 0.000 abstract 1
- -1 phospho- Chemical class 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 125000001424 substituent group Chemical group 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cosmetics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Secuencia de ácidos nucleicos aislada, que codifica un polipéptido con actividad desaturasa, seleccionada entre el grupo: a) una secuencia de ácidos nucleicos con la secuencia representada en la SEC ID N° 2 o la SEC ID N° 7 o la SEC ID N° 5; o b) secuencias de ácidos nucleicos, que por retrotraducción en una secuencia de ácidos nucleicos se derivan de la secuencia de aminoácidos representada en la SEC ID N° 3 o la SEC ID N° 8 o la SEC ID N° 6, en base al código genético degenerado; o c) derivados de la secuencia de ácidos nucleicos representada en la SEC ID N° 2 o la SEC ID N° 7 o la SEC ID N° 5, los cuales codifican los polipéptidos con las secuencias de aminoácidos representadas en la SEC ID N° 3 o la SEC ID N° 8 o la SEC ID N° 6 y presentan una identidad de por lo menos 75% en el nivel de los aminoácidos, sin que se haya modificado sustancialmente el efecto enzimático de los polipéptidos. Constructo de ácidos nucleicos que contiene dicha secuencia de ácidos nucleicos, en donde la secuencia de ácidos nucleicos está unida con una o más senales de regulación; y vector que contiene la secuencia de ácidos nucleicos o un constructo de ácidos nucleicos. Organismo que contiene por lo menos la secuencia de ácidos nucleicos, por lo menos el constructo de ácidos nucleicos o por lo menos el vector. El organismo puede ser una planta, un microorganismo o un animal. Procedimiento para la preparación de aceites o triglicéridos con un contenido aumentado de ácidos grasos insaturados y aceites o triglicéridos obtenidos mediante el mismo. Uso de la secuencia de ácidos nucleicos aislada o un constructo de ácidos nucleicos para la preparación de plantas transgénicas, y uso de una secuencia de ácidos nucleicos aislada o un fragmento de la misma para el aislamiento de una secuencia genómica por medio de rastreo de homología. Uso de triglicéridos o mezclas de ácidos grasos con un contenido aumentado de ácidos grasos insaturados para la preparación de nutrientes, alimentos para animales, cosméticos o productos farmacéuticos. Secuencia de ácidos nucleicos aislados, que codifica para una proteína, que transforma un ácido graso de la estructura general (1) que presenta dos enlaces dobles separados entre sí por un grupo metileno, en un ácido graso triplemente insaturado de la estructura general (2) en donde los tres enlaces dobles del ácido graso están en conjugación y en donde los sustituyentes y variables en los compuestos de la estructura general (1) y (2) tienen el siguiente significado: R1 = hidrógeno, alquilo C1-10 ramificado o no ramificado, insaturado o saturado, sustituido o no sustituido, ó un resto de fórmula (3); R2 = alquilo C1-9 insaturado o saturado, sustituido o no sustituido; R3 y R4, independientemente entre sí, hidrógeno, alquilcarbonilo C1-22- o fosfo-, ramificado o no ramificado, saturado o insaturado, sustituido o no sustituido; y n = 1 a 14.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10134660A DE10134660A1 (de) | 2001-07-20 | 2001-07-20 | Fettsäure-Desaturase-Gene aus Granatapfel und Verfahren zur Herstellung von ungesättigten Fettsäuren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR036178A1 true AR036178A1 (es) | 2004-08-18 |
Family
ID=7692035
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP020102694A AR036178A1 (es) | 2001-07-20 | 2002-07-18 | Genes de la desaturasa del acido graso de la granada y procedimiento para la preparacion de acidos grasos insaturados |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20050166271A1 (es) |
| EP (1) | EP1412489A2 (es) |
| AR (1) | AR036178A1 (es) |
| CA (1) | CA2454372A1 (es) |
| DE (1) | DE10134660A1 (es) |
| WO (1) | WO2003012091A2 (es) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1437412A4 (en) * | 2001-09-20 | 2005-02-02 | Plantech Res Inst | GENES INVOLVED IN THE SYNTHESIS OF FATTY ACID WITH A TRANS-11, CIS-13-CONJUGATED DOUBLE BINDING AND USE THEREOF |
| CN102559364B (zh) | 2004-04-22 | 2016-08-17 | 联邦科学技术研究组织 | 用重组细胞合成长链多不饱和脂肪酸 |
| EP1756280B2 (en) * | 2004-04-22 | 2024-01-17 | Commonwealth Scientific and Industrial Research Organisation | Synthesis of long-chain polyunsaturated fatty acids by recombinant cells |
| JP2008523821A (ja) * | 2004-12-20 | 2008-07-10 | ビーエーエスエフ プラント サイエンス ゲーエムベーハー | 植物由来の脂肪酸デサチュラーゼ遺伝子をコードする核酸分子およびその使用方法 |
| RU2009111266A (ru) | 2006-08-29 | 2010-10-10 | Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU) | Синтез жирных кислот |
| US20090018186A1 (en) * | 2006-09-06 | 2009-01-15 | The Coca-Cola Company | Stable beverage products comprising polyunsaturated fatty acid emulsions |
| US20080058418A1 (en) * | 2006-09-06 | 2008-03-06 | The Coca-Cola Company | Stable polyunsaturated fatty acid emulsions and methods for inhibiting, suppressing, or reducing degradation of polyunsaturated fatty acids in an emulsion |
| BR122021003836B1 (pt) | 2008-11-18 | 2022-02-01 | Commonwealth Scientific And Industrial Research Organisation | Polinucleotídeo isolado e/ou exógeno que não ocorre de forma natural, vetor e método para produzir óleo contendo ácidos graxos insaturados |
| SG11201408362SA (en) | 2012-06-15 | 2015-01-29 | Commw Scient Ind Res Org | Production of long chain polyunsaturated fatty acids in plant cells |
| NZ721036A (en) | 2013-12-18 | 2023-07-28 | Grains Res & Dev Corp | Lipid comprising long chain polyunsaturated fatty acids |
| EP3160482A4 (en) | 2014-06-27 | 2018-02-14 | Commonwealth Scientific and Industrial Research Organisation | Lipid comprising docosapentaenoic acid |
| CN110283931B (zh) * | 2019-07-12 | 2022-04-26 | 安徽省农业科学院园艺研究所 | 6个安徽淮北石榴优良品种的ssr指纹图谱及其构建方法与应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3356699A (en) * | 1965-06-09 | 1967-12-05 | Marvin O Bagby | Alkali isomerization of crepenynic acid to 8, 10, 12-octadecatrienoic acid |
| US4164505A (en) * | 1977-07-08 | 1979-08-14 | Sylvachem Corporation | Flow process for conjugating unconjugated unsaturation of fatty acids |
| JP3313724B2 (ja) * | 1993-12-28 | 2002-08-12 | 麒麟麦酒株式会社 | 脂肪酸の不飽和化酸素遺伝子，当該遺伝子を含むベクター，当該遺伝子が導入された植物及びその作出方法 |
| US6075183A (en) * | 1997-04-11 | 2000-06-13 | Abbott Laboratories | Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants |
| DE19941609A1 (de) * | 1999-09-01 | 2001-03-08 | Inst Pflanzenbiochemie Ipb | Fettsäure-Desaturase-Gen aus Pflanzen |
-
2001
- 2001-07-20 DE DE10134660A patent/DE10134660A1/de not_active Withdrawn
-
2002
- 2002-07-09 CA CA002454372A patent/CA2454372A1/en not_active Abandoned
- 2002-07-09 EP EP02791448A patent/EP1412489A2/de not_active Withdrawn
- 2002-07-09 WO PCT/EP2002/007611 patent/WO2003012091A2/de not_active Application Discontinuation
- 2002-07-09 US US10/484,202 patent/US20050166271A1/en not_active Abandoned
- 2002-07-18 AR ARP020102694A patent/AR036178A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1412489A2 (de) | 2004-04-28 |
| CA2454372A1 (en) | 2003-02-13 |
| US20050166271A1 (en) | 2005-07-28 |
| WO2003012091A2 (de) | 2003-02-13 |
| WO2003012091A3 (de) | 2003-09-12 |
| DE10134660A1 (de) | 2003-02-06 |
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|---|---|---|---|
| FB | Suspension of granting procedure |