AR026186A1 - METHOD FOR BUILDING GENETICALLY MODIFIED BODIES OF STEROID DEGRADING MICROORGANISMS THAT LACK OF THE CAPACITY TO DEGRAD THE STEROIDS NUCLEUS - Google Patents

METHOD FOR BUILDING GENETICALLY MODIFIED BODIES OF STEROID DEGRADING MICROORGANISMS THAT LACK OF THE CAPACITY TO DEGRAD THE STEROIDS NUCLEUS

Info

Publication number
AR026186A1
AR026186A1 ARP000105523A ARP000105523A AR026186A1 AR 026186 A1 AR026186 A1 AR 026186A1 AR P000105523 A ARP000105523 A AR P000105523A AR P000105523 A ARP000105523 A AR P000105523A AR 026186 A1 AR026186 A1 AR 026186A1
Authority
AR
Argentina
Prior art keywords
steroid
steroids
genetically modified
nucleus
degrad
Prior art date
Application number
ARP000105523A
Other languages
Spanish (es)
Original Assignee
Akzo Nobel Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel Nv filed Critical Akzo Nobel Nv
Publication of AR026186A1 publication Critical patent/AR026186A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un método para construir cepas genéticamente modificadas de microorganismos degradadores de esteroides, donde multiples genes involucrados en ladegradacion del nucleo de los esteroides son inactivados, tales como los genes de esteroide deshidrogenasa s. Uno de estos genes es kstD1. Cepas con multiplesgenes de enzimas degradadoras de esteroides inactivados pueden usarse para la acumulacion de esteroides intermediarios con un alto rendimiento. Un producto deacumulacion preferido es 9alfa-hidroxi-4- androsteno-3,17-diona.A method for constructing genetically modified strains of steroid degrading microorganisms, where multiple genes involved in steroid nucleus degradation are inactivated, such as steroid dehydrogenase s genes. One of these genes is kstD1. Strains with multiplesgenes of inactivated steroid degrading enzymes can be used for the accumulation of intermediate steroids with high yield. A preferred accumulation product is 9alpha-hydroxy-4- androstene-3,17-dione.

ARP000105523A 1999-10-22 2000-10-20 METHOD FOR BUILDING GENETICALLY MODIFIED BODIES OF STEROID DEGRADING MICROORGANISMS THAT LACK OF THE CAPACITY TO DEGRAD THE STEROIDS NUCLEUS AR026186A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99203470 1999-10-22
EP99204449 1999-12-22

Publications (1)

Publication Number Publication Date
AR026186A1 true AR026186A1 (en) 2003-01-29

Family

ID=26153382

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP000105523A AR026186A1 (en) 1999-10-22 2000-10-20 METHOD FOR BUILDING GENETICALLY MODIFIED BODIES OF STEROID DEGRADING MICROORGANISMS THAT LACK OF THE CAPACITY TO DEGRAD THE STEROIDS NUCLEUS

Country Status (11)

Country Link
EP (1) EP1232278A1 (en)
CN (1) CN1224716C (en)
AR (1) AR026186A1 (en)
AU (1) AU775476B2 (en)
CA (1) CA2396879A1 (en)
CZ (1) CZ20021784A3 (en)
IL (1) IL149715A0 (en)
NO (1) NO20022449L (en)
NZ (1) NZ519039A (en)
RU (1) RU2268935C2 (en)
WO (1) WO2001031050A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003219134A1 (en) 2002-02-21 2003-09-09 Akzo Nobel N.V. Identification of 3-ketosteroid 9-alfa-hydroxylase genes and microorganisms blocked in 3-ketosteroid 9-alfa-hydroxylase activity
CA2506217A1 (en) * 2002-12-03 2004-07-01 Robert Van Der Geize New expression system from rhodococcus
AT503486B1 (en) * 2006-04-11 2008-05-15 Iep Gmbh METHOD FOR THE ENANTIOSELECTIVE REDUCTION OF STEROIDS
WO2009024572A1 (en) * 2007-08-21 2009-02-26 N.V. Organon Method for the production of modified steroid degrading microorganisms and their use
CN102413876A (en) 2009-02-23 2012-04-11 格罗宁根大学 Pharmaceutical compositions and methods for treating tuberculosis
CN103361394B (en) * 2013-08-07 2016-08-17 中国科学院上海高等研究院 Utilize the method that microorganism converts preparation 9 Alpha-hydroxies-androstenedione
CN103805577A (en) * 2013-08-14 2014-05-21 济南环亿生物科技有限公司 Method for efficiently producing hydroxysteroid dehydrogenase with testosterone comamonas
ES2732749T3 (en) 2014-02-27 2019-11-25 Consejo Superior Investigacion Selective recombinant Mycobacterium smegmatis mc2 155 mutants and their use for the production of 1,4-androstadien-3,17-dione or 4-androsten-3,17-dione from natural sterols
CN107586762A (en) * 2017-09-18 2018-01-16 天津科技大学 A kind of dehydrogenase mutant of 3 sterone Δ 1 and its application
US11001871B2 (en) 2017-12-15 2021-05-11 Jiangnan University Method for producing 9alpha-hydroxy androstane-4-alkene-3,17-diketone by enzymatic conversion
CN107955827B (en) * 2017-12-15 2019-07-02 江南大学 A kind of 9 Alpha-hydroxy androstane-4-alkene-3s of enzymatic conversion method production, the method for 17- diketone
GB2577037A (en) * 2018-08-09 2020-03-18 Cambrex Karlskoga Ab Genetically-modified bacteria and uses thereof
CN110229838B (en) * 2019-05-28 2021-01-08 浙江理工大学 Method for obtaining hydroxylated compound by biotransformation of steroid compound
CN114621965B (en) * 2022-02-15 2023-10-03 复旦大学 3-sterone-delta 1 Dehydrogenase mutants and uses thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU196627B (en) * 1986-11-18 1988-12-28 Gyogyszerkutato Intezet Microbiological process for producing y alpha-hydroxy-4-androstene-3,17-dione

Also Published As

Publication number Publication date
RU2002113373A (en) 2004-02-27
RU2268935C2 (en) 2006-01-27
NZ519039A (en) 2004-09-24
NO20022449L (en) 2002-06-19
AU775476B2 (en) 2004-08-05
AU1514501A (en) 2001-05-08
CN1224716C (en) 2005-10-26
EP1232278A1 (en) 2002-08-21
WO2001031050A1 (en) 2001-05-03
IL149715A0 (en) 2002-11-10
CA2396879A1 (en) 2001-05-03
CN1413260A (en) 2003-04-23
NO20022449D0 (en) 2002-05-23
CZ20021784A3 (en) 2002-08-14

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