AR016939A1 - Metodo para seleccionar una vid transgenica o componente de una vid que tiene una resistencia aumentada a la enfermedad de ''fanleaf'' y una celulade planta transformada con un vector de expresion. - Google Patents

Metodo para seleccionar una vid transgenica o componente de una vid que tiene una resistencia aumentada a la enfermedad de ''fanleaf'' y una celulade planta transformada con un vector de expresion.

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Publication number
AR016939A1
AR016939A1 ARP980104828A ARP980104828A AR016939A1 AR 016939 A1 AR016939 A1 AR 016939A1 AR P980104828 A ARP980104828 A AR P980104828A AR P980104828 A ARP980104828 A AR P980104828A AR 016939 A1 AR016939 A1 AR 016939A1
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AR
Argentina
Prior art keywords
vine
transgenic
component
selecting
nucleic acid
Prior art date
Application number
ARP980104828A
Other languages
English (en)
Original Assignee
Cornell Res Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Res Foundation Inc filed Critical Cornell Res Foundation Inc
Publication of AR016939A1 publication Critical patent/AR016939A1/es

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/88Vitaceae, e.g. Vitus [grape]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205Agrobacterium mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/00022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/32011Picornaviridae
    • C12N2770/32022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

Un método para seleccionar una vid transgénica o un componente de la vid que tiene una resistencia aumentada a la enfermedad de fanleaf (hoja enabanico), el método incluye los pasos de: (a) transformar una célula de planta de uva con una molécula deácido nucleico de proteína de revestimiento delnepovirus de la uva o su fragmento que sea capaz de ser expresada en la célula de la planta; (b) regenerar una vid transgénica o el componente de vid de lacélula de la planta; y (c) seleccionar una vid transgénica o componente de vid que expresa, a un nivel bajo, a la molécula de ácido nucleico o su fragmento,donde el nivel bajo de expresion aumenta la resistencia de la vid transgénica o componente de la vid a la enfermedad de fanleaf (hoja en abanico). Lainvencion también se refiere a una molécula de ácido nucleico aislada que codifica a una proteína de revestimiento o su fragmento de una aislado de Genevade un virus de nepovirus de la vid. Y una célula transformada con un vector de expresionque comprende a una molécula de ácido nucleico aislada que codifica auna proteína recombinante que comprende a una secuencia de aminoácido que tiene la menos el 97% de identidad de aminoácido con respecto a la secuenciade amino ácido de la proteína de revestimiento del nepovirus de la vid del aislado Genevadel Nro. de Sec. ID: 2.
ARP980104828A 1997-09-29 1998-09-28 Metodo para seleccionar una vid transgenica o componente de una vid que tiene una resistencia aumentada a la enfermedad de ''fanleaf'' y una celulade planta transformada con un vector de expresion. AR016939A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US6038497P 1997-09-29 1997-09-29

Publications (1)

Publication Number Publication Date
AR016939A1 true AR016939A1 (es) 2001-08-01

Family

ID=22029138

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP980104828A AR016939A1 (es) 1997-09-29 1998-09-28 Metodo para seleccionar una vid transgenica o componente de una vid que tiene una resistencia aumentada a la enfermedad de ''fanleaf'' y una celulade planta transformada con un vector de expresion.

Country Status (17)

Country Link
US (2) US6667426B1 (es)
EP (1) EP1026941B1 (es)
CN (1) CN1280457A (es)
AR (1) AR016939A1 (es)
AT (1) ATE456297T1 (es)
AU (2) AU767778B2 (es)
BG (1) BG104364A (es)
BR (1) BR9812690A (es)
CA (1) CA2304366A1 (es)
DE (1) DE69841478D1 (es)
ES (1) ES2339515T3 (es)
HU (1) HUP0003697A2 (es)
IL (1) IL134967A0 (es)
MD (1) MD20000109A (es)
TR (1) TR200000807T2 (es)
WO (1) WO1999016298A1 (es)
ZA (1) ZA988878B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA200607843B (en) * 2004-02-23 2008-06-25 Israel State Engrafted plants resistant to viral diseases and methods of producing same
WO2005118805A1 (en) * 2004-05-28 2005-12-15 University Of Florida Research Foundation, Inc. Materials and methods for providing resistance to plant pathogens in non-transgenic plant tissue
EP2348815B1 (en) * 2008-10-31 2016-01-27 Cornell University Engineering broad and durable resistance to grapevine fanleaf virus in plants
CL2011002884A1 (es) * 2011-11-16 2012-07-20 Univ Pontificia Catolica Chile Plasmido que comprende secuencias del genoma de virus gflv, celula que comprende dichas construcciones moleculares, y procedimiento para conferir resistencia y saneamiento al virus de la hoja en abanico de la vid (gflv) en vides no transgenicas.
WO2013072827A2 (es) * 2011-11-16 2013-05-23 Pontificia Universidad Catolica De Chile Generación de portainjertos de vid para resistencia y saneamiento al virus de la hoja en abanico de la vid (gflv)
CN115066434A (zh) 2019-12-03 2022-09-16 阿拉玛生物科学公司 核酸连接的免疫-夹心测定(nulisa)
US20230047498A1 (en) * 2019-12-09 2023-02-16 Texas Tech University System Method and compositions for engineering grapevine red blotch virus-resistant grapevine

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USPP6166P (en) * 1986-05-23 1988-05-03 The Regents Of The University Of California Hybrid grapevine rootstock
US5596132A (en) 1990-03-12 1997-01-21 Cornell Research Foundation, Inc. Induction of resistance to virus diseases by transformation of plants with a portion of a plant virus genome involving a read-through replicase gene
US5633449A (en) 1990-03-12 1997-05-27 Cornell Research Foundation, Inc. Induction of resistance to viral diseases in plants
PT626998E (pt) 1992-02-19 2002-04-29 State Of Oregon By S B Hi Educ "producao de plantas resistentes aos virus, mediante introducao de uma molecula de arn viral nao traduzivel e de sentido codificador"
US6232528B1 (en) * 1996-06-26 2001-05-15 University Of Florida Research Foundation Incorporated Disease resistance in vitis

Also Published As

Publication number Publication date
ZA988878B (en) 1999-04-06
MD20000109A (ro) 2001-05-31
WO1999016298A1 (en) 1999-04-08
CA2304366A1 (en) 1999-04-08
US7211710B2 (en) 2007-05-01
AU9778098A (en) 1999-04-23
BG104364A (en) 2001-04-30
US20030226172A1 (en) 2003-12-04
DE69841478D1 (de) 2010-03-18
BR9812690A (pt) 2000-08-22
CN1280457A (zh) 2001-01-17
AU2003262427A1 (en) 2003-12-11
EP1026941A4 (en) 2004-05-26
HUP0003697A2 (hu) 2001-02-28
TR200000807T2 (tr) 2000-11-21
ES2339515T3 (es) 2010-05-20
IL134967A0 (en) 2001-05-20
ATE456297T1 (de) 2010-02-15
EP1026941A1 (en) 2000-08-16
EP1026941B1 (en) 2010-01-27
US6667426B1 (en) 2003-12-23
AU767778B2 (en) 2003-11-27
WO1999016298A9 (en) 1999-07-15

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