ZA200604147B - Reduction of dermal scarring - Google Patents
Reduction of dermal scarring Download PDFInfo
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- ZA200604147B ZA200604147B ZA200604147A ZA200604147A ZA200604147B ZA 200604147 B ZA200604147 B ZA 200604147B ZA 200604147 A ZA200604147 A ZA 200604147A ZA 200604147 A ZA200604147 A ZA 200604147A ZA 200604147 B ZA200604147 B ZA 200604147B
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
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- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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| CN100482674C (zh) * | 2003-11-24 | 2009-04-29 | 坎吉有限公司 | 皮肤瘢痕形成的减少 |
| AU2009205429A1 (en) | 2008-01-14 | 2009-07-23 | Conventus Orthopaedics Inc. | Apparatus and methods for fracture repair |
| US8796443B2 (en) | 2008-09-22 | 2014-08-05 | Rxi Pharmaceuticals Corporation | Reduced size self-delivering RNAi compounds |
| EP2523614A4 (en) | 2010-01-15 | 2017-02-15 | Conventus Orthopaedics, Inc. | Rotary-rigid orthopaedic rod |
| CN105534561B (zh) | 2010-01-20 | 2018-04-03 | 康文图斯整形外科公司 | 用于骨接近和骨腔准备的装置及方法 |
| JP2013521880A (ja) | 2010-03-08 | 2013-06-13 | コンベンタス オーソピディックス， インコーポレイテッド | 骨インプラントを固定するための装置および方法 |
| KR20180044433A (ko) | 2010-03-24 | 2018-05-02 | 알엑스아이 파마슈티칼스 코포레이션 | 진피 및 섬유증성 적응증에서의 ｒｎａ 간섭 |
| EP3077050B1 (en) * | 2013-12-04 | 2020-10-21 | Phio Pharmaceuticals Corp. | Methods for treatment of wound healing utilizing chemically modified oligonucleotides |
| AU2014362251B2 (en) | 2013-12-12 | 2019-10-10 | Conventus Orthopaedics, Inc. | Tissue displacement tools and methods |
| US10900039B2 (en) | 2014-09-05 | 2021-01-26 | Phio Pharmaceuticals Corp. | Methods for treating aging and skin disorders using nucleic acids targeting Tyr or MMP1 |
| SG11201808704YA (en) | 2016-04-14 | 2018-11-29 | Trizell Ltd | Viral vector stabilization |
| WO2019010252A2 (en) | 2017-07-04 | 2019-01-10 | Conventus Orthopaedics, Inc. | Apparatus and methods for treatment of a bone |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4394370A (en) | 1981-09-21 | 1983-07-19 | Jefferies Steven R | Bone graft material for osseous defects and method of making same |
| US4505266A (en) | 1981-10-26 | 1985-03-19 | Massachusetts Institute Of Technology | Method of using a fibrous lattice |
| US4485097A (en) | 1982-05-26 | 1984-11-27 | Massachusetts Institute Of Technology | Bone-equivalent and method for preparation thereof |
| JPH0662679B2 (ja) | 1985-06-21 | 1994-08-17 | 新田ゼラチン株式会社 | 組織親和性コラ−ゲンとその製法 |
| WO1992002247A1 (en) * | 1990-08-01 | 1992-02-20 | Chiron Ophthalmics, Inc. | Method for treating corneal endothelial wounds |
| US5302706A (en) | 1991-12-16 | 1994-04-12 | Baylor College Of Medicine | Senescent cell derived inhibitors of DNA synthesis |
| US6372249B1 (en) | 1991-12-16 | 2002-04-16 | Baylor College Of Medicine | Senscent cell-derived inhibitors of DNA synthesis |
| US5596079A (en) | 1991-12-16 | 1997-01-21 | Smith; James R. | Mimetics of senescent cell derived inhibitors of DNA synthesis |
| KR100331363B1 (ko) | 1992-10-16 | 2002-12-05 | 콜드 스프링 하버 래보레이토리 | 사이클린복합체재배열및그것과관련된용도 |
| US6211334B1 (en) | 1992-10-16 | 2001-04-03 | Cold Spring Harbor | Cell-cycle regulatory proteins, and uses related thereto |
| FR2702152B1 (fr) | 1993-03-03 | 1995-05-24 | Inst Nat Sante Rech Med | Virus recombinants et leur utilisation en thérapie génique. |
| CA2170641C (en) | 1993-08-30 | 2011-11-15 | James R. Smith | Senescent cell-derived inhibitors of dna synthesis |
| TW442569B (en) | 1993-10-25 | 2001-06-23 | Canji Inc | Recombinant adenoviral vector |
| US6210939B1 (en) | 1993-10-25 | 2001-04-03 | Canji, Inc. | Recombinant adenoviral vector and methods of use |
| EP0702730A4 (en) | 1994-03-18 | 1997-05-07 | Univ Utah Res Found | COREWAY MUTTATIONS IN THE MTS GENE AND METHODS FOR DETECTING AN APPROXIMATION OF CANCER IN THE MTS GENE |
| ATE283355T1 (de) | 1994-05-24 | 2004-12-15 | Baylor College Medicine | Mimetika von aus alternden zellen abgeleitete hemmer der dna synthese |
| US5851806A (en) * | 1994-06-10 | 1998-12-22 | Genvec, Inc. | Complementary adenoviral systems and cell lines |
| US5631156A (en) | 1994-06-21 | 1997-05-20 | The University Of Michigan | DNA encoding and 18 KD CDK6 inhibiting protein |
| WO1996012506A1 (en) | 1994-10-24 | 1996-05-02 | Baylor College Of Medecine | Senescent cell-derived inhibitors of dna synthesis |
| US5827702A (en) | 1994-10-31 | 1998-10-27 | Genentech, Inc. | Ocular gene therapy |
| GB9422175D0 (en) | 1994-11-03 | 1994-12-21 | Univ Dundee | Indentification of the p21 waf1-pcna interaction site and therapeutic applications thereof |
| AU4602396A (en) | 1994-12-22 | 1996-07-10 | Regents Of The University Of Michigan, The | Dna encoding a 20 kd cdk6 inhibiting protein |
| US5837520A (en) | 1995-03-07 | 1998-11-17 | Canji, Inc. | Method of purification of viral vectors |
| US5856094A (en) | 1995-05-12 | 1999-01-05 | The Johns Hopkins University School Of Medicine | Method of detection of neoplastic cells |
| US6027742A (en) | 1995-05-19 | 2000-02-22 | Etex Corporation | Bioresorbable ceramic composites |
| US5756283A (en) | 1995-06-05 | 1998-05-26 | The Trustees Of The University Of Pennsylvania | Method for improved production of recombinant adeno-associated viruses for gene therapy |
| HUP9901908A3 (en) | 1995-07-17 | 2000-02-28 | Univ Texas | P16 expression constructs and their application in cancer therapy |
| GB9519275D0 (en) | 1995-09-21 | 1995-11-22 | Univ Dundee | Substances and their therapeutic use |
| US5863904A (en) | 1995-09-26 | 1999-01-26 | The University Of Michigan | Methods for treating cancers and restenosis with P21 |
| US5837511A (en) * | 1995-10-02 | 1998-11-17 | Cornell Research Foundation, Inc. | Non-group C adenoviral vectors |
| US6001853A (en) * | 1996-01-26 | 1999-12-14 | The United States Of America As Represented By The Department Of Health And Human Services | Hydroxylamine compositions for the prevention or retardation of cataracts |
| AU726584B2 (en) | 1996-04-10 | 2000-11-09 | University Of Southern California | Gene therapy for proliferative vitreoretinopathy |
| IT1285790B1 (it) * | 1996-09-24 | 1998-06-24 | Angeletti P Ist Richerche Bio | Adenovirus difettivi ricombinanti che codificano per mutanti di interleuchina 6 umana (hil-6) con attivita' antagonista o |
| US6274614B1 (en) | 1997-02-11 | 2001-08-14 | Qlt Inc. | Methods, compositions and articles for reducing or preventing the effects of inflammation |
| JPH11205102A (ja) | 1998-01-13 | 1999-07-30 | Mitsubishi Electric Corp | 遅延同期回路 |
| US6489305B1 (en) | 1998-05-08 | 2002-12-03 | Canji, Inc. | Methods and compositions for the treatment of ocular diseases |
| US6395029B1 (en) * | 1999-01-19 | 2002-05-28 | The Children's Hospital Of Philadelphia | Sustained delivery of polyionic bioactive agents |
| AU6919300A (en) * | 1999-08-20 | 2001-03-19 | University Of Pittsburgh | Methods for in vivo gene delivery to sites of cartilage damage |
| US6579522B1 (en) * | 2000-06-27 | 2003-06-17 | Genvec, Inc. | Replication deficient adenoviral TNF vector |
| US6667173B2 (en) | 2000-12-01 | 2003-12-23 | The Schepens Eye Research Institute | Nucleic acids encoding platelet derived growth factor-alpha receptors |
| CN100482674C (zh) * | 2003-11-24 | 2009-04-29 | 坎吉有限公司 | 皮肤瘢痕形成的减少 |
| US7947267B2 (en) * | 2004-10-08 | 2011-05-24 | Potentia Pharmaceuticals, Inc. | Viral complement control proteins for eye disorders |
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- 2004-11-23 BR BRPI0416920-4A patent/BRPI0416920A/pt active Search and Examination
- 2004-11-23 CN CN2009101287188A patent/CN101507822B/zh not_active Expired - Fee Related
- 2004-11-23 AU AU2004293474A patent/AU2004293474B2/en not_active Ceased
- 2004-11-23 EP EP04812200A patent/EP1692154A4/en not_active Withdrawn
- 2004-11-23 WO PCT/US2004/039633 patent/WO2005051971A1/en not_active Ceased
- 2004-11-23 KR KR1020067012618A patent/KR101167330B1/ko not_active Expired - Fee Related
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- 2004-11-23 US US10/997,769 patent/US7465442B2/en not_active Expired - Fee Related
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2006
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| IL175603A (en) | 2011-02-28 |
| NZ546983A (en) | 2009-05-31 |
| EP1692154A4 (en) | 2009-07-08 |
| CN101507822B (zh) | 2012-06-06 |
| JP2007512377A (ja) | 2007-05-17 |
| AU2004293474A1 (en) | 2005-06-09 |
| JP4842139B2 (ja) | 2011-12-21 |
| US7465442B2 (en) | 2008-12-16 |
| WO2005051971A1 (en) | 2005-06-09 |
| US20090142397A1 (en) | 2009-06-04 |
| NO20062978L (no) | 2006-06-26 |
| AU2004293474B2 (en) | 2010-12-23 |
| CN101507822A (zh) | 2009-08-19 |
| US20050271623A1 (en) | 2005-12-08 |
| US20110129534A1 (en) | 2011-06-02 |
| CN100482674C (zh) | 2009-04-29 |
| US8329671B2 (en) | 2012-12-11 |
| BRPI0416920A (pt) | 2007-01-23 |
| JP2011207912A (ja) | 2011-10-20 |
| EP1692154A1 (en) | 2006-08-23 |
| CA2546287C (en) | 2014-04-22 |
| KR101167330B1 (ko) | 2012-07-23 |
| CA2546287A1 (en) | 2005-06-09 |
| CN1906205A (zh) | 2007-01-31 |
| IL175603A0 (en) | 2006-09-05 |
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