ZA200602938B - Cloning of cytochrome p450 genes from Nicotiana - Google Patents

Cloning of cytochrome p450 genes from Nicotiana Download PDF

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Publication number
ZA200602938B
ZA200602938B ZA200602938A ZA200602938A ZA200602938B ZA 200602938 B ZA200602938 B ZA 200602938B ZA 200602938 A ZA200602938 A ZA 200602938A ZA 200602938 A ZA200602938 A ZA 200602938A ZA 200602938 B ZA200602938 B ZA 200602938B
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seq
nucleic acid
plant
amino acid
acid seq
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ZA200602938A
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English (en)
Inventor
Dongmei Xu
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Us Smokeless Tobacco Company
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Publication date
Priority claimed from US10/686,947 external-priority patent/US20040162420A1/en
Application filed by Us Smokeless Tobacco Company filed Critical Us Smokeless Tobacco Company
Publication of ZA200602938B publication Critical patent/ZA200602938B/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042NADPH-cytochrome P450 reductase (1.6.2.4)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ZA200602938A 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from Nicotiana ZA200602938B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/686,947 US20040162420A1 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana

Publications (1)

Publication Number Publication Date
ZA200602938B true ZA200602938B (en) 2008-06-25

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ID=37426131

Family Applications (1)

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ZA200602938A ZA200602938B (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from Nicotiana

Country Status (4)

Country Link
US (3) US8058504B2 (fr)
EP (1) EP2330191A1 (fr)
CN (2) CN1926235B (fr)
ZA (1) ZA200602938B (fr)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
CN1926235B (zh) 2003-10-16 2012-07-18 美国无烟烟草有限责任公司 从烟草克隆细胞色素p450基因
US8637731B2 (en) * 2003-10-16 2014-01-28 U.S. Smokeless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
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US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
EP1851317B1 (fr) 2005-02-23 2011-10-26 North Carolina State University Alteration de la teneur en alcaloide de tabac par modification des genes cytochrome p450 specifiques
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
KR100794395B1 (ko) * 2007-01-19 2008-01-15 명지대학교 산학협력단 식물의 종자 크기를 증가시키는 시토크롬 p450 유전자
EP2220231B1 (fr) 2007-11-12 2017-10-11 North Carolina State University Réduction de la teneur du tabac en alcaloïdes par modification de gènes spécifiques du cytochrome p450
WO2011088180A1 (fr) 2010-01-15 2011-07-21 North Carolina State University Compositions et procédés pour réduire au minimum la synthèse de nornicotine dans le tabac
KR20140034160A (ko) 2011-02-28 2014-03-19 알트리아 클라이언트 서비시스 인코포레이티드 담배 근친교배 식물체 albex1f 및 albex1ms
RU2013139871A (ru) 2011-02-28 2015-04-10 Норт Каролина Стейт Юниверсити Инбредные растения табака ncbex1f, ncbex1mc и nc ex90
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EP2964016A1 (fr) 2013-03-05 2016-01-13 North Carolina State University Plantes autogames et hybrides de tabac et leurs utilisations
EP3113602A1 (fr) 2014-03-03 2017-01-11 North Carolina State University Plants de tabac autogames et hybrides et produits de tabac obtenus à partir de ceux-ci
EP3113603A1 (fr) 2014-03-03 2017-01-11 North Carolina State University Plantes de tabac autogames et hybrides et produits de tabac fabriqués à partir de celles-ci
EP3113604A1 (fr) 2014-03-03 2017-01-11 North Carolina State University Plantes hybrides et autogames de tabac et produits de tabac fabriqués à partir de celles-ci
KR102524798B1 (ko) * 2014-09-26 2023-04-24 필립모리스 프로덕츠 에스.에이. 니트레이트 동화 경로의 변경을 통한 담배 특이적 니트로소아민의 감소
CN113774035A (zh) * 2021-09-01 2021-12-10 广东省农业科学院果树研究所 一种细胞色素P450酶及其编码基因CsCYP2与应用

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Also Published As

Publication number Publication date
US8658856B2 (en) 2014-02-25
US20120233727A1 (en) 2012-09-13
US20100261192A1 (en) 2010-10-14
US8188337B2 (en) 2012-05-29
CN1926235A (zh) 2007-03-07
CN1926235B (zh) 2012-07-18
EP2330191A1 (fr) 2011-06-08
CN1867674A (zh) 2006-11-22
US20120011622A1 (en) 2012-01-12
CN1867674B (zh) 2011-11-09
US8058504B2 (en) 2011-11-15

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