CA2362603A1 - Polynucleotides et polypeptides dwf4 et leurs utilisations - Google Patents

Polynucleotides et polypeptides dwf4 et leurs utilisations Download PDF

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Publication number
CA2362603A1
CA2362603A1 CA002362603A CA2362603A CA2362603A1 CA 2362603 A1 CA2362603 A1 CA 2362603A1 CA 002362603 A CA002362603 A CA 002362603A CA 2362603 A CA2362603 A CA 2362603A CA 2362603 A1 CA2362603 A1 CA 2362603A1
Authority
CA
Canada
Prior art keywords
dwf4
polynucleotide
plant
sequence
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002362603A
Other languages
English (en)
Inventor
Ricardo Azpiroz
Sunghwa Choe
Kenneth A. Feldmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arizona Board of Regents of University of Arizona
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2362603A1 publication Critical patent/CA2362603A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8229—Meristem-specific, e.g. nodal, apical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8233—Female-specific, e.g. pistil, ovule
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8298—Brassinosteroids

Abstract

L'invention concerne des polynucléotides isolés à partir de plantes naines. Ces polynucléotides dwf4 codent tout ou partie d'un polypeptide DWF4, une enzyme cytochrome P450 qui assure la médiation d'étapes multiples dans la synthèse des brassinostéroïdes. L'invention concerne également des polynucléotides isolés codant des régions régulatrices de dwf4. L'invention concerne en outre les utilisations de ces polypeptides et polynucléotides DOLLAR i (dwf4.)
CA002362603A 1999-02-11 2000-02-11 Polynucleotides et polypeptides dwf4 et leurs utilisations Abandoned CA2362603A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11965799P 1999-02-11 1999-02-11
US11965899P 1999-02-11 1999-02-11
US60/119,658 1999-02-11
US60/119,657 1999-02-11
PCT/US2000/003820 WO2000047715A2 (fr) 1999-02-11 2000-02-11 Polynucleotides et polypeptides dwf4 et leurs utilisations

Publications (1)

Publication Number Publication Date
CA2362603A1 true CA2362603A1 (fr) 2000-08-17

Family

ID=26817553

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002362603A Abandoned CA2362603A1 (fr) 1999-02-11 2000-02-11 Polynucleotides et polypeptides dwf4 et leurs utilisations

Country Status (4)

Country Link
EP (1) EP1173547A4 (fr)
AU (1) AU4001000A (fr)
CA (1) CA2362603A1 (fr)
WO (1) WO2000047715A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6987025B1 (en) 1999-02-11 2006-01-17 The Arizona Board Of Regents On Behalf Of The University Of Arizona Dwf4 polynucleotides, polypeptides and uses thereof
CA2447503A1 (fr) 2001-05-16 2002-11-21 The Arizona Board Of Regents On Behalf Of The University Of Arizona Dwf12 et ses mutants
CN1926235B (zh) * 2003-10-16 2012-07-18 美国无烟烟草有限责任公司 从烟草克隆细胞色素p450基因
WO2005111216A2 (fr) * 2004-04-23 2005-11-24 Ceres Inc. Procédés pour modifier des caractéristiques de plantes
EP2166086A3 (fr) * 2005-12-01 2010-10-20 CropDesign N.V. Installations dotées de caractéristiques de croissance améliorées et procédés de fabrication de celles-ci
CA2836815A1 (fr) * 2011-05-20 2012-11-29 Frontier Agri-Science Inc. Plantes ayant une resistance au stress abiotique accrue
CN109609541B (zh) * 2018-11-27 2021-10-19 杭州瑞丰生物科技有限公司 一种改良农作物性状的方法
CN109913465A (zh) * 2019-03-12 2019-06-21 天津大学 佛甲草耐Ni基因SlDWF4及其应用
CN110628737B (zh) * 2019-10-14 2022-06-07 南京农业大学 一个调控黄瓜矮化性状相关基因及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5952545A (en) * 1996-03-27 1999-09-14 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Nucleic acid molecules encoding cytochrome P450-type proteins involved in the brassinosteroid synthesis in plants

Also Published As

Publication number Publication date
WO2000047715A3 (fr) 2001-11-15
WO2000047715A2 (fr) 2000-08-17
WO2000047715A9 (fr) 2001-08-02
EP1173547A4 (fr) 2004-09-15
AU4001000A (en) 2000-08-29
EP1173547A2 (fr) 2002-01-23

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued