ZA200504212B - Method for generating a genetically modified organism for screening active substances. - Google Patents

Method for generating a genetically modified organism for screening active substances. Download PDF

Info

Publication number
ZA200504212B
ZA200504212B ZA200504212A ZA200504212A ZA200504212B ZA 200504212 B ZA200504212 B ZA 200504212B ZA 200504212 A ZA200504212 A ZA 200504212A ZA 200504212 A ZA200504212 A ZA 200504212A ZA 200504212 B ZA200504212 B ZA 200504212B
Authority
ZA
South Africa
Prior art keywords
organism
expression
protein
gene
compensatingly
Prior art date
Application number
ZA200504212A
Other languages
English (en)
Inventor
Bert Klebl
Anja Stadler
Rosemarie Soellner
Ekkehard Leberer
Almut Nitsche
Original Assignee
Aventis Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Gmbh filed Critical Aventis Pharma Gmbh
Publication of ZA200504212B publication Critical patent/ZA200504212B/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1072Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1079Screening libraries by altering the phenotype or phenotypic trait of the host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082Supracellular entities, e.g. tissue, organisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Hematology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
ZA200504212A 2002-12-17 2005-05-24 Method for generating a genetically modified organism for screening active substances. ZA200504212B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10258885A DE10258885A1 (de) 2002-12-17 2002-12-17 Verfahren zur Generierung eines gentechnisch veränderten Organismus

Publications (1)

Publication Number Publication Date
ZA200504212B true ZA200504212B (en) 2006-02-22

Family

ID=32519000

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200504212A ZA200504212B (en) 2002-12-17 2005-05-24 Method for generating a genetically modified organism for screening active substances.

Country Status (14)

Country Link
US (1) US20040143854A1 (no)
EP (1) EP1576186B1 (no)
JP (1) JP4630067B2 (no)
KR (2) KR20120038025A (no)
CN (1) CN1726290B (no)
AT (1) ATE508201T1 (no)
AU (1) AU2003283409A1 (no)
CA (1) CA2509333A1 (no)
DE (2) DE10258885A1 (no)
HK (1) HK1087734A1 (no)
NO (1) NO20053344D0 (no)
RU (1) RU2376364C2 (no)
WO (1) WO2004055206A1 (no)
ZA (1) ZA200504212B (no)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
WO2006014899A2 (en) * 2004-07-26 2006-02-09 Dow Global Technologies Inc. Process for improved protein expression by strain engineering
JP2007063225A (ja) * 2005-09-01 2007-03-15 Takeda Chem Ind Ltd イミダゾピリジン化合物
JP2009515997A (ja) * 2005-11-18 2009-04-16 タケダ サン ディエゴ インコーポレイテッド グルコキナーゼ活性剤
JP5302012B2 (ja) 2006-03-08 2013-10-02 タケダ カリフォルニア インコーポレイテッド グルコキナーゼ活性剤
WO2007124115A2 (en) * 2006-04-20 2007-11-01 Trustees Of Boston College Compositions and methods for identifying inhibitors and activators of cyclic amp phosphodiesterases
WO2007143434A2 (en) * 2006-05-31 2007-12-13 Takeda San Diego, Inc. Indazole and isoindole derivatives as glucokinase activating agents
US8163779B2 (en) 2006-12-20 2012-04-24 Takeda San Diego, Inc. Glucokinase activators
US8173645B2 (en) * 2007-03-21 2012-05-08 Takeda San Diego, Inc. Glucokinase activators
AU2008245696B2 (en) 2007-04-27 2013-11-07 Pelican Technology Holdings, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2632250A1 (en) * 2010-10-29 2013-09-04 F.Hoffmann-La Roche Ag Murine model of inflammation with il33 n-terminal domain deletion
HUP1100657A2 (en) * 2011-11-29 2013-06-28 Eotvos Lorand Tudomanyegyetem Transgenic caenorhabditis elegans
CN110172454B (zh) * 2019-05-23 2020-11-13 浙江大学 一种s-腺苷甲硫氨酸合成酶突变体及其高通量筛选方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2188950C (en) * 1994-04-26 2001-07-03 James R. Broach Functional expression of mammalian adenylyl cyclase in yeast
CA2277414A1 (en) * 1997-11-07 1999-05-20 Georges Natsoulis Surrogate genetics target characterization method
AU2596800A (en) * 1998-12-31 2000-07-31 Iconix Pharmaceuticals, Inc. Method for generating a pathway reporter system
WO2001046403A1 (en) * 1999-12-22 2001-06-28 Iconix Pharmaceuticals, Inc. Synthetic lethal expression screen
CA2437383A1 (en) * 2001-02-02 2002-08-15 Raymond Kim Alteration of phenotype due to heterologous genes
US20030044866A1 (en) * 2001-08-15 2003-03-06 Charles Boone Yeast arrays, methods of making such arrays, and methods of analyzing such arrays

Also Published As

Publication number Publication date
AU2003283409A1 (en) 2004-07-09
KR101215854B1 (ko) 2012-12-31
HK1087734A1 (en) 2006-10-20
NO20053344L (no) 2005-07-08
WO2004055206A1 (de) 2004-07-01
JP2006509512A (ja) 2006-03-23
NO20053344D0 (no) 2005-07-08
ATE508201T1 (de) 2011-05-15
US20040143854A1 (en) 2004-07-22
KR20050085806A (ko) 2005-08-29
EP1576186B1 (de) 2011-05-04
DE10258885A1 (de) 2004-07-15
KR20120038025A (ko) 2012-04-20
CA2509333A1 (en) 2004-07-01
DE50313671D1 (de) 2011-06-16
RU2005122470A (ru) 2006-05-10
JP4630067B2 (ja) 2011-02-09
RU2376364C2 (ru) 2009-12-20
CN1726290A (zh) 2006-01-25
EP1576186A1 (de) 2005-09-21
CN1726290B (zh) 2012-09-05

Similar Documents

Publication Publication Date Title
ZA200504212B (en) Method for generating a genetically modified organism for screening active substances.
Suhm et al. Mitochondrial translation efficiency controls cytoplasmic protein homeostasis
Meimoun et al. Degradation of the transcription factor Gcn4 requires the kinase Pho85 and the SCFCDC4 ubiquitin–ligase complex
Bulik et al. Chitin synthesis in Saccharomyces cerevisiae in response to supplementation of growth medium with glucosamine and cell wall stress
Spence et al. Cell cycle–regulated modification of the ribosome by a variant multiubiquitin chain
García et al. Genomic profiling of fungal cell wall-interfering compounds: identification of a common gene signature
Kafadar et al. Negative regulation of calcineurin signaling by Hrr25p, a yeast homolog of casein kinase I
Clarke et al. Distinct roles for the essential MYST family HAT Esa1p in transcriptional silencing
Hoffmann et al. Long-chain polyprenols promote spore wall formation in Saccharomyces cerevisiae
Santos et al. Sbe2p and sbe22p, two homologous Golgi proteins involved in yeast cell wall formation
Hosiner et al. Arsenic toxicity to Saccharomyces cerevisiae is a consequence of inhibition of the TORC1 kinase combined with a chronic stress response
Chen et al. The Ccr4-Not complex regulates TORC1 signaling and mitochondrial metabolism by promoting vacuole V-ATPase activity
Latterich et al. Isolation and characterization of osmosensitive vacuolar mutants of Saccharomyces cerevisiae
Nomura et al. Methylglyoxal activates Gcn2 to phosphorylate eIF2α independently of the TOR pathway in Saccharomyces cerevisiae
Tatip et al. Changes in transcription start sites of Zap1‐regulated genes during zinc deficiency: Implications for HNT1 gene regulation
Milgrom et al. TFIID and Spt-Ada-Gcn5-acetyltransferase functions probed by genome-wide synthetic genetic array analysis using a Saccharomyces cerevisiae taf9-ts allele
JPWO2008156048A1 (ja) 酵母細胞セット、標的候補分子同定方法、作用機序解析方法及びスクリーニング方法
Busti et al. Overexpression of Far1, a cyclin-dependent kinase inhibitor, induces a large transcriptional reprogramming in which RNA synthesis senses Far1 in a Sfp1-mediated way
Phadnis et al. Role of the putative structural protein Sed1p in mitochondrial genome maintenance
EP1445327B1 (en) Method of screening drug acting on cell wall
Umeda et al. Overexpression of polyphosphate polymerases and deletion of polyphosphate phosphatases shorten the replicative lifespan in yeast
Vallabhaneni Saccharomyces cerevisiae synthesizes ribosomal RNA using RNA polymerase II during nitrogen deprivation
Hwang et al. Fus3 and Tpk2 protein kinases regulate the phosphorylation-dependent functions of RNA helicase Dhh1 in yeast mating and Ste12 protein expression
Ámon et al. Role of High Mobility Group B protein HmbA, orthologue of yeast Nhp6p in Aspergillus nidulans
Nagar DNA Damage Response Activates the Electron Transport Chain and Oxidative Metabolism by Two Parallel Mechanisms