ZA200404737B - Nano-composite martensitic steels - Google Patents
Nano-composite martensitic steels Download PDFInfo
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- ZA200404737B ZA200404737B ZA200404737A ZA200404737A ZA200404737B ZA 200404737 B ZA200404737 B ZA 200404737B ZA 200404737 A ZA200404737 A ZA 200404737A ZA 200404737 A ZA200404737 A ZA 200404737A ZA 200404737 B ZA200404737 B ZA 200404737B
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22C—ALLOYS
- C22C38/00—Ferrous alloys, e.g. steel alloys
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22C—ALLOYS
- C22C38/00—Ferrous alloys, e.g. steel alloys
- C22C38/18—Ferrous alloys, e.g. steel alloys containing chromium
- C22C38/40—Ferrous alloys, e.g. steel alloys containing chromium with nickel
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22C—ALLOYS
- C22C38/00—Ferrous alloys, e.g. steel alloys
- C22C38/18—Ferrous alloys, e.g. steel alloys containing chromium
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22C—ALLOYS
- C22C38/00—Ferrous alloys, e.g. steel alloys
- C22C38/18—Ferrous alloys, e.g. steel alloys containing chromium
- C22C38/40—Ferrous alloys, e.g. steel alloys containing chromium with nickel
- C22C38/58—Ferrous alloys, e.g. steel alloys containing chromium with nickel with more than 1.5% by weight of manganese
-
- C—CHEMISTRY; METALLURGY
- C21—METALLURGY OF IRON
- C21D—MODIFYING THE PHYSICAL STRUCTURE OF FERROUS METALS; GENERAL DEVICES FOR HEAT TREATMENT OF FERROUS OR NON-FERROUS METALS OR ALLOYS; MAKING METAL MALLEABLE, e.g. BY DECARBURISATION OR TEMPERING
- C21D2201/00—Treatment for obtaining particular effects
- C21D2201/03—Amorphous or microcrystalline structure
-
- C—CHEMISTRY; METALLURGY
- C21—METALLURGY OF IRON
- C21D—MODIFYING THE PHYSICAL STRUCTURE OF FERROUS METALS; GENERAL DEVICES FOR HEAT TREATMENT OF FERROUS OR NON-FERROUS METALS OR ALLOYS; MAKING METAL MALLEABLE, e.g. BY DECARBURISATION OR TEMPERING
- C21D2211/00—Microstructure comprising significant phases
- C21D2211/001—Austenite
-
- C—CHEMISTRY; METALLURGY
- C21—METALLURGY OF IRON
- C21D—MODIFYING THE PHYSICAL STRUCTURE OF FERROUS METALS; GENERAL DEVICES FOR HEAT TREATMENT OF FERROUS OR NON-FERROUS METALS OR ALLOYS; MAKING METAL MALLEABLE, e.g. BY DECARBURISATION OR TEMPERING
- C21D2211/00—Microstructure comprising significant phases
- C21D2211/008—Martensite
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Mechanical Engineering (AREA)
- Metallurgy (AREA)
- Organic Chemistry (AREA)
- Heat Treatment Of Steel (AREA)
- Heat Treatment Of Sheet Steel (AREA)
- Heat Treatment Of Strip Materials And Filament Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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| US10/017,879 US6709534B2 (en) | 2001-12-14 | 2001-12-14 | Nano-composite martensitic steels |
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| ZA200404737B true ZA200404737B (en) | 2006-12-27 |
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| US20040149362A1 (en) * | 2002-11-19 | 2004-08-05 | Mmfx Technologies Corporation, A Corporation Of The State Of California | Cold-worked steels with packet-lath martensite/austenite microstructure |
| US20060065327A1 (en) * | 2003-02-07 | 2006-03-30 | Advance Steel Technology | Fine-grained martensitic stainless steel and method thereof |
| US6890393B2 (en) * | 2003-02-07 | 2005-05-10 | Advanced Steel Technology, Llc | Fine-grained martensitic stainless steel and method thereof |
| US6899773B2 (en) * | 2003-02-07 | 2005-05-31 | Advanced Steel Technology, Llc | Fine-grained martensitic stainless steel and method thereof |
| US20070228729A1 (en) * | 2003-03-06 | 2007-10-04 | Grimmett Harold M | Tubular goods with threaded integral joint connections |
| US20060006648A1 (en) * | 2003-03-06 | 2006-01-12 | Grimmett Harold M | Tubular goods with threaded integral joint connections |
| US7169239B2 (en) | 2003-05-16 | 2007-01-30 | Lone Star Steel Company, L.P. | Solid expandable tubular members formed from very low carbon steel and method |
| US7214278B2 (en) * | 2004-12-29 | 2007-05-08 | Mmfx Technologies Corporation | High-strength four-phase steel alloys |
| CN1328406C (zh) * | 2005-06-22 | 2007-07-25 | 宁波浙东精密铸造有限公司 | 一种薄膜奥氏体增韧的马氏体耐磨铸钢及其制造方法 |
| CN100357460C (zh) * | 2006-03-14 | 2007-12-26 | 钢铁研究总院 | 一种获得多元组织马氏体钢的冷却工艺 |
| EP2044228B1 (en) * | 2006-06-29 | 2010-05-19 | Tenaris Connections AG | Seamless precision steel tubes with improved isotropic toughness at low temperature for hydraulic cylinders and process for obtaining the same |
| RU2360029C1 (ru) * | 2008-01-09 | 2009-06-27 | Открытое акционерное общество "Научно-исследовательский институт металлургической технологии" | Высокопрочная немагнитная композиционная сталь |
| EP2325435B2 (en) | 2009-11-24 | 2020-09-30 | Tenaris Connections B.V. | Threaded joint sealed to [ultra high] internal and external pressures |
| US20110236696A1 (en) * | 2010-03-25 | 2011-09-29 | Winky Lai | High strength rebar |
| US9163296B2 (en) | 2011-01-25 | 2015-10-20 | Tenaris Coiled Tubes, Llc | Coiled tube with varying mechanical properties for superior performance and methods to produce the same by a continuous heat treatment |
| IT1403688B1 (it) | 2011-02-07 | 2013-10-31 | Dalmine Spa | Tubi in acciaio con pareti spesse con eccellente durezza a bassa temperatura e resistenza alla corrosione sotto tensione da solfuri. |
| IT1403689B1 (it) | 2011-02-07 | 2013-10-31 | Dalmine Spa | Tubi in acciaio ad alta resistenza con eccellente durezza a bassa temperatura e resistenza alla corrosione sotto tensioni da solfuri. |
| US8414715B2 (en) | 2011-02-18 | 2013-04-09 | Siderca S.A.I.C. | Method of making ultra high strength steel having good toughness |
| US8636856B2 (en) | 2011-02-18 | 2014-01-28 | Siderca S.A.I.C. | High strength steel having good toughness |
| FI20115702L (fi) * | 2011-07-01 | 2013-01-02 | Rautaruukki Oyj | Menetelmä suurlujuuksisen rakenneteräksen valmistamiseksi ja suurlujuuksinen rakenneteräs |
| JP5910168B2 (ja) * | 2011-09-15 | 2016-04-27 | 臼井国際産業株式会社 | Trip型2相マルテンサイト鋼及びその製造方法とそのtrip型2相マルテンサイト鋼を用いた超高強度鋼製加工品 |
| US9340847B2 (en) | 2012-04-10 | 2016-05-17 | Tenaris Connections Limited | Methods of manufacturing steel tubes for drilling rods with improved mechanical properties, and rods made by the same |
| RU2495141C1 (ru) * | 2012-05-11 | 2013-10-10 | Федеральное Государственное Бюджетное Образовательное Учреждение Высшего Профессионального Образования "Донской Государственный Технический Университет" (Дгту) | Способ получения естественного феррито-мартенситного композита |
| CN102703837B (zh) * | 2012-05-25 | 2014-05-14 | 燕山大学 | 一种纳米结构板条马氏体钢及其制备方法 |
| GB2525337B (en) | 2013-01-11 | 2016-06-22 | Tenaris Connections Ltd | Galling resistant drill pipe tool joint and corresponding drill pipe |
| US9187811B2 (en) | 2013-03-11 | 2015-11-17 | Tenaris Connections Limited | Low-carbon chromium steel having reduced vanadium and high corrosion resistance, and methods of manufacturing |
| US8978430B2 (en) | 2013-03-13 | 2015-03-17 | Commercial Metals Company | System and method for stainless steel cladding of carbon steel pieces |
| US9803256B2 (en) | 2013-03-14 | 2017-10-31 | Tenaris Coiled Tubes, Llc | High performance material for coiled tubing applications and the method of producing the same |
| EP2789701A1 (en) | 2013-04-08 | 2014-10-15 | DALMINE S.p.A. | High strength medium wall quenched and tempered seamless steel pipes and related method for manufacturing said steel pipes |
| EP2789700A1 (en) | 2013-04-08 | 2014-10-15 | DALMINE S.p.A. | Heavy wall quenched and tempered seamless steel pipes and related method for manufacturing said steel pipes |
| JP6144417B2 (ja) | 2013-06-25 | 2017-06-07 | テナリス・コネクシヨンズ・ベー・ブイ | 高クロム耐熱鋼 |
| US20160305192A1 (en) | 2015-04-14 | 2016-10-20 | Tenaris Connections Limited | Ultra-fine grained steels having corrosion-fatigue resistance |
| US11124852B2 (en) | 2016-08-12 | 2021-09-21 | Tenaris Coiled Tubes, Llc | Method and system for manufacturing coiled tubing |
| CN106282495B (zh) * | 2016-10-27 | 2018-03-27 | 贵州大学 | 中高碳铬钒钢中形成微纳尺度孪晶马氏体的工艺方法 |
| KR101899670B1 (ko) | 2016-12-13 | 2018-09-17 | 주식회사 포스코 | 저온역 버링성이 우수한 고강도 복합조직강 및 그 제조방법 |
| US10434554B2 (en) | 2017-01-17 | 2019-10-08 | Forum Us, Inc. | Method of manufacturing a coiled tubing string |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4170499A (en) | 1977-08-24 | 1979-10-09 | The Regents Of The University Of California | Method of making high strength, tough alloy steel |
| US4170497A (en) | 1977-08-24 | 1979-10-09 | The Regents Of The University Of California | High strength, tough alloy steel |
| US4619714A (en) | 1984-08-06 | 1986-10-28 | The Regents Of The University Of California | Controlled rolling process for dual phase steels and application to rod, wire, sheet and other shapes |
| US4671827A (en) | 1985-10-11 | 1987-06-09 | Advanced Materials And Design Corp. | Method of forming high-strength, tough, corrosion-resistant steel |
| US5180450A (en) * | 1990-06-05 | 1993-01-19 | Ferrous Wheel Group Inc. | High performance high strength low alloy wrought steel |
| SU1749307A1 (ru) * | 1990-10-30 | 1992-07-23 | Центральный научно-исследовательский институт черной металлургии им.И.П.Бардина | Сталь |
| US5545269A (en) * | 1994-12-06 | 1996-08-13 | Exxon Research And Engineering Company | Method for producing ultra high strength, secondary hardening steels with superior toughness and weldability |
| US5545270A (en) * | 1994-12-06 | 1996-08-13 | Exxon Research And Engineering Company | Method of producing high strength dual phase steel plate with superior toughness and weldability |
| US6264760B1 (en) * | 1997-07-28 | 2001-07-24 | Exxonmobil Upstream Research Company | Ultra-high strength, weldable steels with excellent ultra-low temperature toughness |
| US6159312A (en) * | 1997-12-19 | 2000-12-12 | Exxonmobil Upstream Research Company | Ultra-high strength triple phase steels with excellent cryogenic temperature toughness |
| UA73311C2 (uk) * | 1999-07-12 | 2005-07-15 | Ммфекс Стил Корпорейшн Оф Америка | Низьковуглецеві сталі з високими механічними і корозійними властивостями та спосіб їх виготовлення |
| CN1079447C (zh) * | 1999-11-30 | 2002-02-20 | 河北工业大学 | 无界面碳化物低碳马氏体高强度钢 |
| CN1120247C (zh) * | 2000-02-02 | 2003-09-03 | 燕山大学 | 纳米晶粒低合金钢板的制造方法 |
| JP2001234286A (ja) * | 2000-02-24 | 2001-08-28 | Nippon Steel Corp | 伸線加工性に優れた細径高炭素低合金鋼熱間圧延線材とその製造方法 |
| CN1107728C (zh) * | 2000-04-25 | 2003-05-07 | 钢铁研究总院 | 一种合金结构钢的晶粒超细化方法 |
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2001
- 2001-12-14 US US10/017,879 patent/US6709534B2/en not_active Expired - Lifetime
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2002
- 2002-12-12 KR KR10-2004-7009227A patent/KR20040081434A/ko not_active Ceased
- 2002-12-12 ES ES02792396T patent/ES2309219T3/es not_active Expired - Lifetime
- 2002-12-12 PT PT02792396T patent/PT1461466E/pt unknown
- 2002-12-12 KR KR1020087031418A patent/KR20090007500A/ko not_active Ceased
- 2002-12-12 AT AT02792396T patent/ATE402272T1/de not_active IP Right Cessation
- 2002-12-12 ZA ZA200404737A patent/ZA200404737B/en unknown
- 2002-12-12 JP JP2003553019A patent/JP4776167B2/ja not_active Expired - Fee Related
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2003
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