ZA200301284B - Improved insecticil bacteria, and methods for making and using them. - Google Patents
Improved insecticil bacteria, and methods for making and using them. Download PDFInfo
- Publication number
- ZA200301284B ZA200301284B ZA200301284A ZA200301284A ZA200301284B ZA 200301284 B ZA200301284 B ZA 200301284B ZA 200301284 A ZA200301284 A ZA 200301284A ZA 200301284 A ZA200301284 A ZA 200301284A ZA 200301284 B ZA200301284 B ZA 200301284B
- Authority
- ZA
- South Africa
- Prior art keywords
- sequence
- seq
- polypeptide
- promoter
- protein
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 107
- 241000894006 Bacteria Species 0.000 title claims description 30
- 108700012359 toxins Proteins 0.000 claims description 235
- 108090000623 proteins and genes Proteins 0.000 claims description 216
- 239000003053 toxin Substances 0.000 claims description 180
- 231100000765 toxin Toxicity 0.000 claims description 180
- 102000004169 proteins and genes Human genes 0.000 claims description 157
- 241000193386 Lysinibacillus sphaericus Species 0.000 claims description 127
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 125
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 125
- 229920001184 polypeptide Polymers 0.000 claims description 124
- 239000002773 nucleotide Substances 0.000 claims description 114
- 150000007523 nucleic acids Chemical group 0.000 claims description 111
- 230000001580 bacterial effect Effects 0.000 claims description 97
- 125000003729 nucleotide group Chemical group 0.000 claims description 90
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 61
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 59
- 230000027455 binding Effects 0.000 claims description 56
- 231100000419 toxicity Toxicity 0.000 claims description 54
- 230000001988 toxicity Effects 0.000 claims description 54
- 102000039446 nucleic acids Human genes 0.000 claims description 47
- 108020004707 nucleic acids Proteins 0.000 claims description 47
- 241000255925 Diptera Species 0.000 claims description 33
- 238000004519 manufacturing process Methods 0.000 claims description 33
- 231100000331 toxic Toxicity 0.000 claims description 33
- 230000002588 toxic effect Effects 0.000 claims description 33
- 239000013604 expression vector Substances 0.000 claims description 29
- 230000006870 function Effects 0.000 claims description 29
- 239000000843 powder Substances 0.000 claims description 21
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 15
- 230000001965 increasing effect Effects 0.000 claims description 15
- 241000193365 Bacillus thuringiensis serovar israelensis Species 0.000 claims description 13
- 101100168995 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cyt-1 gene Proteins 0.000 claims description 10
- 101150023613 mev-1 gene Proteins 0.000 claims description 10
- 230000001131 transforming effect Effects 0.000 claims description 9
- 241000894007 species Species 0.000 claims description 7
- 101710198277 RNA polymerase sigma factor sigA Proteins 0.000 claims description 6
- 230000002708 enhancing effect Effects 0.000 claims description 4
- 101001023095 Anemonia sulcata Delta-actitoxin-Avd1a Proteins 0.000 claims 1
- 101000641989 Araneus ventricosus Kunitz-type U1-aranetoxin-Av1a Proteins 0.000 claims 1
- 101001028691 Carybdea rastonii Toxin CrTX-A Proteins 0.000 claims 1
- 101000685083 Centruroides infamatus Beta-toxin Cii1 Proteins 0.000 claims 1
- 101000685085 Centruroides noxius Toxin Cn1 Proteins 0.000 claims 1
- 101001028688 Chironex fleckeri Toxin CfTX-1 Proteins 0.000 claims 1
- 101000644407 Cyriopagopus schmidti U6-theraphotoxin-Hs1a Proteins 0.000 claims 1
- 101000679608 Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) Cysteine rich necrotrophic effector Tox1 Proteins 0.000 claims 1
- 101150103955 isaB gene Proteins 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 description 129
- 210000004027 cell Anatomy 0.000 description 125
- 108091033319 polynucleotide Proteins 0.000 description 41
- 239000002157 polynucleotide Substances 0.000 description 41
- 102000040430 polynucleotide Human genes 0.000 description 41
- 239000013078 crystal Substances 0.000 description 19
- 230000000853 biopesticidal effect Effects 0.000 description 17
- 239000013598 vector Substances 0.000 description 17
- 238000004166 bioassay Methods 0.000 description 16
- 230000000749 insecticidal effect Effects 0.000 description 16
- 150000001413 amino acids Chemical group 0.000 description 14
- 230000000694 effects Effects 0.000 description 14
- 239000013612 plasmid Substances 0.000 description 14
- 241000238631 Hexapoda Species 0.000 description 13
- 238000004422 calculation algorithm Methods 0.000 description 13
- 230000009977 dual effect Effects 0.000 description 13
- 239000012528 membrane Substances 0.000 description 13
- 241000256054 Culex <genus> Species 0.000 description 11
- 230000028070 sporulation Effects 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 238000013518 transcription Methods 0.000 description 11
- 230000035897 transcription Effects 0.000 description 11
- 239000002158 endotoxin Substances 0.000 description 10
- 239000000203 mixture Substances 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 235000014469 Bacillus subtilis Nutrition 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 230000009466 transformation Effects 0.000 description 9
- 230000014616 translation Effects 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 101710112984 20 kDa protein Proteins 0.000 description 8
- 101710159910 Movement protein Proteins 0.000 description 8
- 101710090398 Viral interleukin-10 homolog Proteins 0.000 description 8
- 108091008324 binding proteins Proteins 0.000 description 8
- 102000023732 binding proteins Human genes 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 235000001014 amino acid Nutrition 0.000 description 7
- 229940024606 amino acid Drugs 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 108020004999 messenger RNA Proteins 0.000 description 7
- 235000013336 milk Nutrition 0.000 description 7
- 239000008267 milk Substances 0.000 description 7
- 210000004080 milk Anatomy 0.000 description 7
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 7
- 238000011144 upstream manufacturing Methods 0.000 description 7
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 6
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 6
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 6
- 108091081024 Start codon Proteins 0.000 description 6
- 230000003993 interaction Effects 0.000 description 6
- 238000006467 substitution reaction Methods 0.000 description 6
- 230000001629 suppression Effects 0.000 description 6
- 108020004465 16S ribosomal RNA Proteins 0.000 description 5
- 108091026890 Coding region Proteins 0.000 description 5
- 101710151559 Crystal protein Proteins 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 230000001186 cumulative effect Effects 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 241000256186 Anopheles <genus> Species 0.000 description 4
- 101000634115 Arabidopsis thaliana RNA polymerase sigma factor sigE, chloroplastic/mitochondrial Proteins 0.000 description 4
- 241001071944 Cyta Species 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- 101710097340 RNA polymerase sigma factor RpoD Proteins 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 230000000996 additive effect Effects 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 230000001876 chaperonelike Effects 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 239000002917 insecticide Substances 0.000 description 4
- 231100000636 lethal dose Toxicity 0.000 description 4
- 239000008176 lyophilized powder Substances 0.000 description 4
- 210000000110 microvilli Anatomy 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000013615 primer Substances 0.000 description 4
- 238000003259 recombinant expression Methods 0.000 description 4
- 210000004708 ribosome subunit Anatomy 0.000 description 4
- 108010070880 sigma K Proteins 0.000 description 4
- 241000256057 Culex quinquefasciatus Species 0.000 description 3
- 241000256113 Culicidae Species 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 102100024368 Inositol polyphosphate 5-phosphatase K Human genes 0.000 description 3
- 108091023045 Untranslated Region Proteins 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 229940097012 bacillus thuringiensis Drugs 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- 229960003276 erythromycin Drugs 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 231100000171 higher toxicity Toxicity 0.000 description 3
- 230000002147 killing effect Effects 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000000386 microscopy Methods 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 230000008520 organization Effects 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 230000005180 public health Effects 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 230000000087 stabilizing effect Effects 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 238000011179 visual inspection Methods 0.000 description 3
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108700003918 Bacillus Thuringiensis insecticidal crystal Proteins 0.000 description 2
- 241001327104 Bacillus thuringiensis serovar medellin Species 0.000 description 2
- 241000149420 Bothrometopus brevis Species 0.000 description 2
- 241000902363 Chrysomela scripta Species 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 102100030011 Endoribonuclease Human genes 0.000 description 2
- 108010093099 Endoribonucleases Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 201000006353 Filariasis Diseases 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- 241000258916 Leptinotarsa decemlineata Species 0.000 description 2
- 241000186779 Listeria monocytogenes Species 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- 241000256103 Simuliidae Species 0.000 description 2
- 241000256251 Spodoptera frugiperda Species 0.000 description 2
- 108700009124 Transcription Initiation Site Proteins 0.000 description 2
- 241000255993 Trichoplusia ni Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 230000003042 antagnostic effect Effects 0.000 description 2
- 235000003704 aspartic acid Nutrition 0.000 description 2
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 239000008121 dextrose Substances 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000001666 entomocidal effect Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 238000002744 homologous recombination Methods 0.000 description 2
- 230000006801 homologous recombination Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 201000004792 malaria Diseases 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000002231 mosquitocidal effect Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 239000006916 nutrient agar Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000000159 protein binding assay Methods 0.000 description 2
- 231100000654 protein toxin Toxicity 0.000 description 2
- 150000003230 pyrimidines Chemical class 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 244000000000 soil microbiome Species 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 101150082072 14 gene Proteins 0.000 description 1
- 241000256111 Aedes <genus> Species 0.000 description 1
- 241001425390 Aphis fabae Species 0.000 description 1
- 229920003319 Araldite® Polymers 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000557722 Bacillus thuringiensis serovar jegathesan Species 0.000 description 1
- 241001147758 Bacillus thuringiensis serovar kurstaki Species 0.000 description 1
- 241000193369 Bacillus thuringiensis serovar tenebrionis Species 0.000 description 1
- 231100000699 Bacterial toxin Toxicity 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 241000256059 Culex pipiens Species 0.000 description 1
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 208000001490 Dengue Diseases 0.000 description 1
- 206010012310 Dengue fever Diseases 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 241001522878 Escherichia coli B Species 0.000 description 1
- 102000002464 Galactosidases Human genes 0.000 description 1
- 108010093031 Galactosidases Proteins 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241001147381 Helicoverpa armigera Species 0.000 description 1
- 241000256257 Heliothis Species 0.000 description 1
- 101000835862 Homo sapiens Mothers against decapentaplegic homolog 1 Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 102100025744 Mothers against decapentaplegic homolog 1 Human genes 0.000 description 1
- 101000774651 Naja atra Zinc metalloproteinase-disintegrin-like kaouthiagin-like Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- SZRPVOFXFLWAPX-UHFFFAOYSA-K P(=O)([O-])([O-])O.[Ca+2].[Cl-].[Rb+] Chemical compound P(=O)([O-])([O-])O.[Ca+2].[Cl-].[Rb+] SZRPVOFXFLWAPX-UHFFFAOYSA-K 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 239000000688 bacterial toxin Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000004534 cecum Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 101150086784 cry gene Proteins 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 101150041801 cyt1Ab1 gene Proteins 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 208000025729 dengue disease Diseases 0.000 description 1
- 238000000326 densiometry Methods 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 231100000676 disease causative agent Toxicity 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000001516 effect on protein Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- TYZRDJZSCBLHSS-UHFFFAOYSA-N ethanol;2-methyloxirane Chemical compound CCO.CC1CO1 TYZRDJZSCBLHSS-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- -1 formamide or DMSO Chemical class 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 238000013492 plasmid preparation Methods 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 239000002719 pyrimidine nucleotide Substances 0.000 description 1
- 125000000714 pyrimidinyl group Chemical group 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000012865 response to insecticide Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000013605 shuttle vector Substances 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009044 synergistic interaction Effects 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 230000024033 toxin binding Effects 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 238000004627 transmission electron microscopy Methods 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/639,576 US6720167B1 (en) | 2000-08-14 | 2000-08-14 | Insecticidal bacteria, and methods for making and using them |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200301284B true ZA200301284B (en) | 2004-02-17 |
Family
ID=24564677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200301284A ZA200301284B (en) | 2000-08-14 | 2003-02-17 | Improved insecticil bacteria, and methods for making and using them. |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US6720167B1 (es) |
| EP (1) | EP1311163A2 (es) |
| JP (1) | JP2004505643A (es) |
| KR (1) | KR20030027028A (es) |
| AU (1) | AU2001286492A1 (es) |
| BR (1) | BR0113284A (es) |
| CA (1) | CA2419157A1 (es) |
| CR (1) | CR6905A (es) |
| HU (1) | HUP0302676A3 (es) |
| IL (1) | IL154382A0 (es) |
| MX (1) | MXPA03001358A (es) |
| WO (1) | WO2002013606A2 (es) |
| ZA (1) | ZA200301284B (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050183161A1 (en) * | 2003-10-14 | 2005-08-18 | Athenix Corporation | AXMI-010, a delta-endotoxin gene and methods for its use |
| JP5145506B2 (ja) * | 2006-08-25 | 2013-02-20 | 福岡県 | バシラス属細菌、及びその用途 |
| AU2009231626A1 (en) * | 2008-04-04 | 2009-10-08 | Insectigen, Inc. | Novel protein for binding Bacillus thuringiensis Cry toxins and fragments of cadherins for enhancing Cry toxicity against dipterans |
| KR101453437B1 (ko) * | 2009-01-30 | 2014-10-21 | 한국생명공학연구원 | 유전자 발현을 증대시키는 신규한 핵산분자 및 이를 이용한 단백질 생산방법 |
| US20110243906A1 (en) * | 2010-04-05 | 2011-10-06 | Park Hyun-Woo | Strain of highly mosquitocidal bacillus |
| MY178859A (en) * | 2011-01-31 | 2020-10-21 | Entogenex Ind Sdn Bhd | Bioinsecticide and method for producing thereof |
| CN102816816B (zh) * | 2012-08-29 | 2014-01-29 | 西安交通大学 | 一种球形芽孢杆菌伴胞晶体蛋白制备方法及其应用 |
| US10743535B2 (en) | 2017-08-18 | 2020-08-18 | H&K Solutions Llc | Insecticide for flight-capable pests |
| CN110786293B (zh) * | 2019-11-01 | 2021-11-30 | 中国人民解放军陆军军医大学 | 能够导致斯氏按蚊传疟能力增强的生物杀虫剂的使用方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
-
2000
- 2000-08-14 US US09/639,576 patent/US6720167B1/en not_active Expired - Fee Related
-
2001
- 2001-08-14 CA CA002419157A patent/CA2419157A1/en not_active Abandoned
- 2001-08-14 JP JP2002518760A patent/JP2004505643A/ja not_active Withdrawn
- 2001-08-14 HU HU0302676A patent/HUP0302676A3/hu unknown
- 2001-08-14 WO PCT/US2001/025543 patent/WO2002013606A2/en not_active Application Discontinuation
- 2001-08-14 AU AU2001286492A patent/AU2001286492A1/en not_active Abandoned
- 2001-08-14 IL IL15438201A patent/IL154382A0/xx unknown
- 2001-08-14 MX MXPA03001358A patent/MXPA03001358A/es not_active Application Discontinuation
- 2001-08-14 EP EP01965942A patent/EP1311163A2/en not_active Withdrawn
- 2001-08-14 KR KR10-2003-7002212A patent/KR20030027028A/ko not_active Application Discontinuation
- 2001-08-14 BR BR0113284-9A patent/BR0113284A/pt not_active Application Discontinuation
-
2003
- 2003-02-14 CR CR6905A patent/CR6905A/es not_active Application Discontinuation
- 2003-02-17 ZA ZA200301284A patent/ZA200301284B/en unknown
- 2003-12-26 US US10/746,062 patent/US20050123519A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| KR20030027028A (ko) | 2003-04-03 |
| WO2002013606A3 (en) | 2002-06-06 |
| MXPA03001358A (es) | 2005-09-08 |
| BR0113284A (pt) | 2003-07-08 |
| US20050123519A1 (en) | 2005-06-09 |
| US6720167B1 (en) | 2004-04-13 |
| CA2419157A1 (en) | 2002-02-21 |
| CR6905A (es) | 2003-12-04 |
| HUP0302676A2 (hu) | 2004-04-28 |
| HUP0302676A3 (en) | 2004-11-29 |
| EP1311163A2 (en) | 2003-05-21 |
| JP2004505643A (ja) | 2004-02-26 |
| AU2001286492A1 (en) | 2002-02-25 |
| IL154382A0 (en) | 2003-09-17 |
| WO2002013606A2 (en) | 2002-02-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Feitelson | The Bacillus thuringiensis family tree | |
| EP0792363B2 (en) | Novel pesticidal proteins and strains | |
| Federici et al. | Cyt1Aa protein of Bacillus thuringiensis is toxic to the cottonwood leaf beetle, Chrysomela scripta, and suppresses high levels of resistance to Cry3Aa | |
| US5827514A (en) | Pesticidal compositions | |
| Park et al. | Recombinant larvicidal bacteria with markedly improved efficacy against Culex vectors of West Nile virus | |
| JP3794548B2 (ja) | 活性が改良されたバチルス・チューリンギエンシスＣｒｙ６毒素 | |
| US6720167B1 (en) | Insecticidal bacteria, and methods for making and using them | |
| WO2010077672A2 (en) | Control of mosquito larvae with bti toxins and tmof | |
| Park et al. | Construction and characterization of a recombinant Bacillus thuringiensis subsp. israelensis strain that produces Cry11B | |
| US5489432A (en) | Bacillus thuringiensis isolates active against cockroaches and genes encoding cockroach-active toxins | |
| González-Ponce et al. | Recombinant Bacillus thuringiensis subsp. kurstaki HD73 strain that synthesizes Cry1Ac and chimeric ChiA74∆ sp chitinase inclusions | |
| EP1143800B1 (en) | Biological control of nematodes | |
| US20220243169A1 (en) | Bacillus Thuringiensis Strain | |
| KR20180050245A (ko) | 살충 활성이 있는 바실러스 투린지엔시스 아종 이스라엘렌시스 cab575 균주 및 이의 용도 | |
| EP0767610A1 (en) | Bacillus thuringiensis isolates active against cockroaches | |
| AU711493B2 (en) | Improved production of insecticidal proteins | |
| WO1998002039A1 (en) | Bacillus thuringiensis strains showing improved production of certain lepidopteran-toxic crystal proteins | |
| Bulla et al. | Mosquitocidal Toxin Gene of Bacillus Thuringiensis Subspecies Israelensis | |
| US10472397B2 (en) | Use of Cyt1Aa as broad spectrum targeting domain for insecticidal proteins with basic research and biotechnology applications | |
| KR100432140B1 (ko) | 토양으로부터 분리된 바실러스 투린지엔시스 케이-1 균주 및 이 균주를 이용한 미생물 살충제 | |
| Shakoori et al. | Cloning and expression of cry11B gene from a local isolate of Bacillus thuringiensis and its mosquitocidal activity | |
| WO2022144904A1 (en) | Anti-pest toxins and cells | |
| Raymond et al. | Transfer of the Bacillus thuringiensis israelensis Mosquiticidal Toxin Gene into Mosquito Larval Food Sources | |
| Moar | Characterization and toxicity of Bacillus thuringiensis subsp. kurstaki proteins and spores active against Spodoptera exigua | |
| Roh et al. | Expression and Characterization of a Recombinant Cry 1 Ac Crystal Protein Fused with Cry11A in Bacillus thuringiensis subsp. kurstaki Cry− B |