ZA200208625B - Assay for detection of transferase enzyme activity in drug screening. - Google Patents
Assay for detection of transferase enzyme activity in drug screening. Download PDFInfo
- Publication number
- ZA200208625B ZA200208625B ZA200208625A ZA200208625A ZA200208625B ZA 200208625 B ZA200208625 B ZA 200208625B ZA 200208625 A ZA200208625 A ZA 200208625A ZA 200208625 A ZA200208625 A ZA 200208625A ZA 200208625 B ZA200208625 B ZA 200208625B
- Authority
- ZA
- South Africa
- Prior art keywords
- udp
- enzyme
- transferase
- translocase
- lipid
- Prior art date
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- 230000008901 benefit Effects 0.000 description 1
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000001177 diphosphate Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000009643 growth defect Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 101150058012 mrcA gene Proteins 0.000 description 1
- 238000004816 paper chromatography Methods 0.000 description 1
- 108010023665 peptidoglycan transpeptidase Proteins 0.000 description 1
- 101150105325 ponA gene Proteins 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- GAPYKZAARZMMGP-UHFFFAOYSA-N pyridin-1-ium;acetate Chemical compound CC(O)=O.C1=CC=NC=C1 GAPYKZAARZMMGP-UHFFFAOYSA-N 0.000 description 1
- 238000007420 radioactive assay Methods 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000005820 transferase reaction Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- G01N2333/245—Escherichia (G)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Endocrinology (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN439MA2000 | 2000-06-08 |
Publications (1)
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| ZA200208625B true ZA200208625B (en) | 2003-08-04 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
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| ZA200208624A ZA200208624B (en) | 2000-06-08 | 2002-10-24 | Assay for detection of translocase enzyme activity in drug screening. |
| ZA200208625A ZA200208625B (en) | 2000-06-08 | 2002-10-24 | Assay for detection of transferase enzyme activity in drug screening. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200208624A ZA200208624B (en) | 2000-06-08 | 2002-10-24 | Assay for detection of translocase enzyme activity in drug screening. |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US20030108969A1 (fr) |
| EP (2) | EP1292701B1 (fr) |
| JP (2) | JP2003535598A (fr) |
| AT (1) | ATE335841T1 (fr) |
| AU (2) | AU2001264483A1 (fr) |
| CA (2) | CA2407718A1 (fr) |
| DE (1) | DE60122141T2 (fr) |
| ES (1) | ES2269413T3 (fr) |
| WO (2) | WO2001094622A1 (fr) |
| ZA (2) | ZA200208624B (fr) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| AU769842B2 (en) * | 1999-04-22 | 2004-02-05 | Astrazeneca Ab | Assay for detecting phospho-N-acetylmuramyl-pentapeptide translocase activity |
| AU2001264483A1 (en) * | 2000-06-08 | 2001-12-17 | Astrazeneca Ab | Assay for detection of translocase enzyme activity in drug screening |
| SE0104101D0 (sv) * | 2001-12-05 | 2001-12-05 | Astrazeneca Ab | New assay |
| SE0104102D0 (sv) * | 2001-12-05 | 2001-12-05 | Astrazeneca Ab | New assay |
| WO2004072607A2 (fr) * | 2003-02-05 | 2004-08-26 | Merck & Co. Inc. | Bioanalyse a grande vitesse de glycosyl transferases |
| US9115358B2 (en) | 2006-08-11 | 2015-08-25 | President And Fellows Of Harvard College | Moenomycin biosynthesis-related compositions and methods of use thereof |
| EP2205074A4 (fr) * | 2007-10-04 | 2013-07-31 | Harvard College | Analogues de la moénomycine, procédés de synthèse et utilisations de ceux-ci |
| US9273084B2 (en) | 2012-04-06 | 2016-03-01 | President And Fellows Of Harvard College | Moenomycin analogs, methods of synthesis, and uses thereof |
| WO2013152279A1 (fr) | 2012-04-06 | 2013-10-10 | President And Fellows Of Harvard College | Procédés chimio-enzymatiques pour synthétiser des analogues de la moénomycine |
| WO2013151697A1 (fr) | 2012-04-06 | 2013-10-10 | President And Fellows Of Harvard College | Procédés et composés pour l'identification d'inhibiteurs de glycosyltransférase |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4568649A (en) * | 1983-02-22 | 1986-02-04 | Immunex Corporation | Immediate ligand detection assay |
| SE9404072D0 (sv) * | 1994-11-24 | 1994-11-24 | Astra Ab | Novel polypeptides |
| US5861318A (en) * | 1994-11-16 | 1999-01-19 | Pharmacia & Upjohn Company | Scintillation proximity assay for N-acetylgalactosaminyltransferase activity |
| US6153381A (en) * | 1997-08-28 | 2000-11-28 | Millennium Pharmaceuticals, Inc. | Screening for antibiotics |
| IL137584A0 (en) * | 1998-02-02 | 2001-07-24 | Univ Princeton | SUBSTRATE ANALOGS FOR MurG, METHODS OF MAKING SAME AND ASSAYS USING SAME |
| NZ507722A (en) * | 1998-05-15 | 2003-05-30 | Astrazeneca Ab | A scintillation proximity assay for the detection of peptidoglycan synthesis |
| EP1105148A4 (fr) * | 1998-08-20 | 2003-02-05 | Incara Pharmaceuticals Corp | Analogues de peptides d'udp-murnac, dosages et kits |
| AU2001264483A1 (en) * | 2000-06-08 | 2001-12-17 | Astrazeneca Ab | Assay for detection of translocase enzyme activity in drug screening |
-
2001
- 2001-06-05 AU AU2001264483A patent/AU2001264483A1/en not_active Abandoned
- 2001-06-05 EP EP01938911A patent/EP1292701B1/fr not_active Expired - Lifetime
- 2001-06-05 ES ES01938911T patent/ES2269413T3/es not_active Expired - Lifetime
- 2001-06-05 CA CA002407718A patent/CA2407718A1/fr not_active Abandoned
- 2001-06-05 WO PCT/SE2001/001271 patent/WO2001094622A1/fr active IP Right Grant
- 2001-06-05 JP JP2002502163A patent/JP2003535598A/ja active Pending
- 2001-06-05 DE DE60122141T patent/DE60122141T2/de not_active Expired - Fee Related
- 2001-06-05 EP EP01938912A patent/EP1292702A1/fr not_active Withdrawn
- 2001-06-05 US US10/275,237 patent/US20030108969A1/en not_active Abandoned
- 2001-06-05 AT AT01938911T patent/ATE335841T1/de not_active IP Right Cessation
- 2001-06-05 CA CA002407934A patent/CA2407934A1/fr not_active Abandoned
- 2001-06-05 WO PCT/SE2001/001272 patent/WO2001094623A1/fr not_active Application Discontinuation
- 2001-06-05 AU AU2001264482A patent/AU2001264482A1/en not_active Abandoned
- 2001-06-05 US US10/275,366 patent/US20030143635A1/en not_active Abandoned
- 2001-06-05 JP JP2002502162A patent/JP2003535597A/ja active Pending
-
2002
- 2002-10-24 ZA ZA200208624A patent/ZA200208624B/en unknown
- 2002-10-24 ZA ZA200208625A patent/ZA200208625B/en unknown
-
2006
- 2006-01-04 US US11/326,486 patent/US20060172361A1/en not_active Abandoned
- 2006-06-12 US US11/451,684 patent/US20060228766A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ATE335841T1 (de) | 2006-09-15 |
| US20060228766A1 (en) | 2006-10-12 |
| AU2001264483A1 (en) | 2001-12-17 |
| WO2001094623A1 (fr) | 2001-12-13 |
| WO2001094622A1 (fr) | 2001-12-13 |
| US20030143635A1 (en) | 2003-07-31 |
| DE60122141T2 (de) | 2007-06-28 |
| US20030108969A1 (en) | 2003-06-12 |
| JP2003535598A (ja) | 2003-12-02 |
| DE60122141D1 (de) | 2006-09-21 |
| EP1292701B1 (fr) | 2006-08-09 |
| EP1292702A1 (fr) | 2003-03-19 |
| JP2003535597A (ja) | 2003-12-02 |
| ES2269413T3 (es) | 2007-04-01 |
| CA2407718A1 (fr) | 2001-12-13 |
| US20060172361A1 (en) | 2006-08-03 |
| AU2001264482A1 (en) | 2001-12-17 |
| CA2407934A1 (fr) | 2001-12-13 |
| EP1292701A1 (fr) | 2003-03-19 |
| ZA200208624B (en) | 2003-08-04 |
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