AU2001264483A1 - Assay for detection of translocase enzyme activity in drug screening - Google Patents

Assay for detection of translocase enzyme activity in drug screening

Info

Publication number
AU2001264483A1
AU2001264483A1 AU2001264483A AU6448301A AU2001264483A1 AU 2001264483 A1 AU2001264483 A1 AU 2001264483A1 AU 2001264483 A AU2001264483 A AU 2001264483A AU 6448301 A AU6448301 A AU 6448301A AU 2001264483 A1 AU2001264483 A1 AU 2001264483A1
Authority
AU
Australia
Prior art keywords
assay
detection
enzyme activity
drug screening
translocase enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001264483A
Inventor
Sunita Maria Desousa
Suresh Solapure
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AstraZeneca AB
Original Assignee
AstraZeneca AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AstraZeneca AB filed Critical AstraZeneca AB
Publication of AU2001264483A1 publication Critical patent/AU2001264483A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention provides methods for assaying the activity of the translocase enzyme and/or transferase enzyme involved in peptidoglycan biosynthesis in bacteria using scintillation proximity assay methodology. The methods are suitable for high throughput screening of potential anti-bacterial drugs.
AU2001264483A 2000-06-08 2001-06-05 Assay for detection of translocase enzyme activity in drug screening Abandoned AU2001264483A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN439/MAS/2000 2000-06-08
IN439MA2000 2000-06-08
PCT/SE2001/001272 WO2001094623A1 (en) 2000-06-08 2001-06-05 Assay for detection of translocase enzyme activity in drug screening

Publications (1)

Publication Number Publication Date
AU2001264483A1 true AU2001264483A1 (en) 2001-12-17

Family

ID=11097174

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2001264483A Abandoned AU2001264483A1 (en) 2000-06-08 2001-06-05 Assay for detection of translocase enzyme activity in drug screening
AU2001264482A Abandoned AU2001264482A1 (en) 2000-06-08 2001-06-05 Assay for detection of transferase enzyme activity in drug screening

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2001264482A Abandoned AU2001264482A1 (en) 2000-06-08 2001-06-05 Assay for detection of transferase enzyme activity in drug screening

Country Status (10)

Country Link
US (4) US20030108969A1 (en)
EP (2) EP1292702A1 (en)
JP (2) JP2003535597A (en)
AT (1) ATE335841T1 (en)
AU (2) AU2001264483A1 (en)
CA (2) CA2407718A1 (en)
DE (1) DE60122141T2 (en)
ES (1) ES2269413T3 (en)
WO (2) WO2001094622A1 (en)
ZA (2) ZA200208625B (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2370469A1 (en) * 1999-04-22 2000-11-02 Astrazeneca Ab Assay for detecting phospho-n-acetylmuramyl-pentapeptide translocase activity
EP1292702A1 (en) * 2000-06-08 2003-03-19 AstraZeneca AB Assay for detection of translocase enzyme activity in drug screening
SE0104101D0 (en) * 2001-12-05 2001-12-05 Astrazeneca Ab New assay
SE0104102D0 (en) * 2001-12-05 2001-12-05 Astrazeneca Ab New assay
EP1592804A4 (en) * 2003-02-05 2007-08-22 Merck & Co Inc High speed assay for glycosyl transferases
WO2008021367A2 (en) 2006-08-11 2008-02-21 President And Fellows Of Harvard College Moenomycin biosynthesis-related compositions and methods of use thereof
EP2205074A4 (en) * 2007-10-04 2013-07-31 Harvard College Moenomycin analogs, methods of synthesis, and uses thereof
WO2013152279A1 (en) 2012-04-06 2013-10-10 President And Fellows Of Harvard College Chemoenzymatic methods for synthesizing moenomycin analogs
US9273084B2 (en) 2012-04-06 2016-03-01 President And Fellows Of Harvard College Moenomycin analogs, methods of synthesis, and uses thereof
EP2850091A1 (en) 2012-04-06 2015-03-25 President and Fellows of Harvard College Methods and compounds for identifying glycosyltransferase inhibitors

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4568649A (en) * 1983-02-22 1986-02-04 Immunex Corporation Immediate ligand detection assay
SE9404072D0 (en) * 1994-11-24 1994-11-24 Astra Ab Novel polypeptides
US5861318A (en) * 1994-11-16 1999-01-19 Pharmacia & Upjohn Company Scintillation proximity assay for N-acetylgalactosaminyltransferase activity
US6153381A (en) * 1997-08-28 2000-11-28 Millennium Pharmaceuticals, Inc. Screening for antibiotics
CA2320228A1 (en) * 1998-02-02 1999-08-05 Min Ge Substrate analogs for murg, methods of making same and assays using same
EP1076719A1 (en) * 1998-05-15 2001-02-21 AstraZeneca AB A scintillation proximity assay for the detection of peptidoglycan synthesis
EP1105148A4 (en) * 1998-08-20 2003-02-05 Incara Pharmaceuticals Corp Analogs of udp-murnac peptides, assays and kits
EP1292702A1 (en) * 2000-06-08 2003-03-19 AstraZeneca AB Assay for detection of translocase enzyme activity in drug screening

Also Published As

Publication number Publication date
EP1292702A1 (en) 2003-03-19
US20060172361A1 (en) 2006-08-03
CA2407934A1 (en) 2001-12-13
JP2003535598A (en) 2003-12-02
JP2003535597A (en) 2003-12-02
ZA200208624B (en) 2003-08-04
DE60122141T2 (en) 2007-06-28
US20030143635A1 (en) 2003-07-31
WO2001094622A1 (en) 2001-12-13
EP1292701A1 (en) 2003-03-19
US20030108969A1 (en) 2003-06-12
AU2001264482A1 (en) 2001-12-17
ZA200208625B (en) 2003-08-04
WO2001094623A1 (en) 2001-12-13
DE60122141D1 (en) 2006-09-21
EP1292701B1 (en) 2006-08-09
ATE335841T1 (en) 2006-09-15
ES2269413T3 (en) 2007-04-01
US20060228766A1 (en) 2006-10-12
CA2407718A1 (en) 2001-12-13

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